Restriction map for
pF1KE2493
(Linear) (Six-base) MAPSORT of: KIEE2493.seq Check: 4480 from: 1 to: 789
KIEE2493.orf
With 182 enzymes: SgfI *
December 2, 2013 11:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 300 789
Size: 164 136 489
Fragments arranged by size:
489 164 136
AlwNI CAG_nnn'CTG
Cuts at: 0 784 789
Size: 784 5
ApaI G_GGCC'C
Cuts at: 0 473 789
Size: 473 316
ApaLI G'TGCA_C
Cuts at: 0 594 789
Size: 594 195
AvaI C'yCGr_G
Cuts at: 0 388 789
Size: 388 401
BanI G'GyrC_C
Cuts at: 0 412 789
Size: 412 377
BanII G_rGCy'C
Cuts at: 0 137 397 473 514 789
Size: 137 260 76 41 275
Fragments arranged by size:
275 260 137 76 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 726 789
Size: 726 63
BbvCI CC'TCA_GC
Cuts at: 0 399 789
Size: 399 390
BciVI GTATCCnnnnn_n'
Cuts at: 0 238 789
Size: 238 551
BglI GCCn_nnn'nGGC
Cuts at: 0 215 789
Size: 215 574
Bme1580I G_kGCm'C
Cuts at: 0 473 598 789
Size: 473 125 191
Fragments arranged by size:
473 191 125
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 156 414 789
Size: 156 258 375
Fragments arranged by size:
375 258 156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 789
Size: 211 578
Bpu10I CC'TnA_GC
Cuts at: 0 399 789
Size: 399 390
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 789
Size: 145 644
BsaI GGTCTCn'nnnn_
Cuts at: 0 464 577 789
Size: 464 113 212
Fragments arranged by size:
464 212 113
BsaHI Gr'CG_yC
Cuts at: 0 218 254 789
Size: 218 36 535
Fragments arranged by size:
535 218 36
BsaWI w'CCGG_w
Cuts at: 0 503 789
Size: 503 286
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 337 367 789
Size: 337 30 422
Fragments arranged by size:
422 337 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 387 532 618 648 789
Size: 387 145 86 30 141
Fragments arranged by size:
387 145 141 86 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 343 444 789
Size: 315 28 101 345
Fragments arranged by size:
345 315 101 28
BsiHKAI G_wGCw'C
Cuts at: 0 137 169 302 598 789
Size: 137 32 133 296 191
Fragments arranged by size:
296 191 137 133 32
BsmI GAATG_Cn'
Cuts at: 0 669 789
Size: 669 120
Bsp1286I G_dGCh'C
Cuts at: 0 137 169 302 397 473 514 598 789
Size: 137 32 133 95 76 41 84 191
Fragments arranged by size:
191 137 133 95 84 76 41 32
BspEI T'CCGG_A
Cuts at: 0 503 789
Size: 503 286
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 365 789
Size: 365 424
BsrBI CCG'CTC
Cuts at: 0 522 789
Size: 522 267
BsrDI GCAATG_nn'
Cuts at: 0 445 789
Size: 445 344
BsrFI r'CCGG_y
Cuts at: 0 653 789
Size: 653 136
BstAPI GCAn_nnn'nTGC
Cuts at: 0 287 784 789
Size: 287 497 5
Fragments arranged by size:
497 287 5
Bsu36I CC'TnA_GG
Cuts at: 0 620 789
Size: 620 169
BtgI C'CryG_G
Cuts at: 0 416 463 789
Size: 416 47 326
Fragments arranged by size:
416 326 47
DraIII CAC_nnn'GTG
Cuts at: 0 338 789
Size: 338 451
EaeI y'GGCC_r
Cuts at: 0 680 789
Size: 680 109
EarI CTCTTCn'nnn_
Cuts at: 0 625 789
Size: 625 164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 105 196 789
Size: 105 91 593
Fragments arranged by size:
593 105 91
EcoICRI GAG'CTC
Cuts at: 0 135 789
Size: 135 654
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 211 300 789
Size: 164 47 89 489
Fragments arranged by size:
489 164 89 47
EcoNI CCTnn'n_nnAGG
Cuts at: 0 361 789
Size: 361 428
EcoO109I rG'GnC_Cy
Cuts at: 0 469 470 781 789
Size: 469 1 311 8
Fragments arranged by size:
469 311 8 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 366 789
Size: 366 423
FspI TGC'GCA
Cuts at: 0 437 789
Size: 437 352
HincII GTy'rAC
Cuts at: 0 150 789
Size: 150 639
MscI TGG'CCA
Cuts at: 0 682 789
Size: 682 107
MspA1I CmG'CkG
Cuts at: 0 371 403 789
Size: 371 32 386
Fragments arranged by size:
386 371 32
NaeI GCC'GGC
Cuts at: 0 655 789
Size: 655 134
NcoI C'CATG_G
Cuts at: 0 416 789
Size: 416 373
NgoMIV G'CCGG_C
Cuts at: 0 653 789
Size: 653 136
NspI r_CATG'y
Cuts at: 0 544 789
Size: 544 245
PspOMI G'GGCC_C
Cuts at: 0 469 789
Size: 469 320
PstI C_TGCA'G
Cuts at: 0 780 789
Size: 780 9
PvuII CAG'CTG
Cuts at: 0 403 789
Size: 403 386
SacI G_AGCT'C
Cuts at: 0 137 789
Size: 137 652
SfcI C'TryA_G
Cuts at: 0 776 789
Size: 776 13
SmlI C'TyrA_G
Cuts at: 0 160 789
Size: 160 629
StuI AGG'CCT
Cuts at: 0 115 310 789
Size: 115 195 479
Fragments arranged by size:
479 195 115
StyI C'CwwG_G
Cuts at: 0 416 578 789
Size: 416 162 211
Fragments arranged by size:
416 211 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 186 789
Size: 186 603
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 414 675 789
Size: 414 261 114
Fragments arranged by size:
414 261 114
Enzymes that do cut:
AcuI AlwNI ApaI ApaLI AvaI BanI BanII BbsI
BbvCI BciVI BglI Bme1580I BplI BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI Bsu36I
BtgI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI HincII MscI MspA1I NaeI NcoI
NgoMIV NspI PspOMI PstI PvuII SacI SfcI SmlI
StuI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BcgI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BsaAI BsaBI BseYI
BsiEI BsiWI BsmBI BspHI BsrGI BssHII BssSI BstBI
BstEII BstXI BstYI BstZ17I BtgZI BtsI ClaI CspCI
DraI DrdI EagI EcoRI EcoRV FseI FspAI HaeII
Hin4I HindIII HpaI KasI KpnI MfeI MluI MmeI
MslI NarI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI