Restriction map for
pF1KE2488
(Linear) (Six-base) MAPSORT of: KIEE2488.seq Check: 7226 from: 1 to: 1080
KIEE2488.orf
With 182 enzymes: SgfI *
October 10, 2013 10:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 1080
Size: 757 323
AleI CACnn'nnGTG
Cuts at: 0 375 1080
Size: 375 705
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 239 1080
Size: 239 841
ApaI G_GGCC'C
Cuts at: 0 93 447 1080
Size: 93 354 633
Fragments arranged by size:
633 354 93
AvaI C'yCGr_G
Cuts at: 0 55 133 438 566 1080
Size: 55 78 305 128 514
Fragments arranged by size:
514 305 128 78 55
BanI G'GyrC_C
Cuts at: 0 369 552 878 1080
Size: 369 183 326 202
Fragments arranged by size:
369 326 202 183
BanII G_rGCy'C
Cuts at: 0 93 134 447 521 1080
Size: 93 41 313 74 559
Fragments arranged by size:
559 313 93 74 41
BbeI G_GCGC'C
Cuts at: 0 882 1080
Size: 882 198
BbsI GAAGACnn'nnnn_
Cuts at: 0 196 1080
Size: 196 884
Bme1580I G_kGCm'C
Cuts at: 0 93 447 555 1080
Size: 93 354 108 525
Fragments arranged by size:
525 354 108 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 556 581 880 949 1080
Size: 319 237 25 299 69 131
Fragments arranged by size:
319 299 237 131 69 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 967 1080
Size: 967 113
BsaI GGTCTCn'nnnn_
Cuts at: 0 143 1080
Size: 143 937
BsaHI Gr'CG_yC
Cuts at: 0 879 1080
Size: 879 201
BsaWI w'CCGG_w
Cuts at: 0 513 1080
Size: 513 567
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 91 121 151 1080
Size: 91 30 30 929
Fragments arranged by size:
929 91 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 131 379 442 703 877 880 1080
Size: 131 248 63 261 174 3 200
Fragments arranged by size:
261 248 200 174 131 63 3
BseYI C'CCAG_C
Cuts at: 0 30 275 449 601 663 1058 1080
Size: 30 245 174 152 62 395 22
Fragments arranged by size:
395 245 174 152 62 30 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 245 1080
Size: 172 73 835
Fragments arranged by size:
835 172 73
BsmBI CGTCTCn'nnnn_
Cuts at: 0 78 601 1080
Size: 78 523 479
Fragments arranged by size:
523 479 78
Bsp1286I G_dGCh'C
Cuts at: 0 93 134 447 521 555 1080
Size: 93 41 313 74 34 525
Fragments arranged by size:
525 313 93 74 41 34
BspEI T'CCGG_A
Cuts at: 0 513 1080
Size: 513 567
BsrBI CCG'CTC
Cuts at: 0 746 939 1080
Size: 746 193 141
Fragments arranged by size:
746 193 141
BsrFI r'CCGG_y
Cuts at: 0 465 1065 1080
Size: 465 600 15
Fragments arranged by size:
600 465 15
Bsu36I CC'TnA_GG
Cuts at: 0 40 1080
Size: 40 1040
DraIII CAC_nnn'GTG
Cuts at: 0 797 1080
Size: 797 283
EaeI y'GGCC_r
Cuts at: 0 836 1080
Size: 836 244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 556 581 757 880 949 1080
Size: 319 237 25 176 123 69 131
Fragments arranged by size:
319 237 176 131 123 69 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 862 1080
Size: 862 218
EcoO109I rG'GnC_Cy
Cuts at: 0 90 170 443 444 660 1080
Size: 90 80 273 1 216 420
Fragments arranged by size:
420 273 216 90 80 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 709 1080
Size: 709 371
FspI TGC'GCA
Cuts at: 0 790 1080
Size: 790 290
HaeII r_GCGC'y
Cuts at: 0 882 903 1080
Size: 882 21 177
Fragments arranged by size:
882 177 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 91 121 123 153 1080
Size: 91 30 2 30 927
Fragments arranged by size:
927 91 30 30 2
KasI G'GCGC_C
Cuts at: 0 878 1080
Size: 878 202
MscI TGG'CCA
Cuts at: 0 838 1080
Size: 838 242
MslI CAynn'nnrTG
Cuts at: 0 375 1080
Size: 375 705
MspA1I CmG'CkG
Cuts at: 0 156 601 804 1005 1080
Size: 156 445 203 201 75
Fragments arranged by size:
445 203 201 156 75
NaeI GCC'GGC
Cuts at: 0 1067 1080
Size: 1067 13
NarI GG'CG_CC
Cuts at: 0 879 1080
Size: 879 201
NgoMIV G'CCGG_C
Cuts at: 0 1065 1080
Size: 1065 15
PasI CC'CwG_GG
Cuts at: 0 548 1080
Size: 548 532
PpuMI rG'GwC_Cy
Cuts at: 0 170 1080
Size: 170 910
PspOMI G'GGCC_C
Cuts at: 0 89 443 1080
Size: 89 354 637
Fragments arranged by size:
637 354 89
PstI C_TGCA'G
Cuts at: 0 218 1046 1080
Size: 218 828 34
Fragments arranged by size:
828 218 34
PvuII CAG'CTG
Cuts at: 0 601 1005 1080
Size: 601 404 75
Fragments arranged by size:
601 404 75
RsrII CG'GwC_CG
Cuts at: 0 511 1080
Size: 511 569
SanDI GG'GwC_CC
Cuts at: 0 170 1080
Size: 170 910
ScaI AGT'ACT
Cuts at: 0 398 1080
Size: 398 682
SfcI C'TryA_G
Cuts at: 0 214 1042 1080
Size: 214 828 38
Fragments arranged by size:
828 214 38
SfoI GGC'GCC
Cuts at: 0 880 1080
Size: 880 200
SmaI CCC'GGG
Cuts at: 0 135 440 568 1080
Size: 135 305 128 512
Fragments arranged by size:
512 305 135 128
SmlI C'TyrA_G
Cuts at: 0 982 1080
Size: 982 98
StuI AGG'CCT
Cuts at: 0 38 1080
Size: 38 1042
StyI C'CwwG_G
Cuts at: 0 243 839 1080
Size: 243 596 241
Fragments arranged by size:
596 243 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 561 1080
Size: 561 519
TatI w'GTAC_w
Cuts at: 0 323 396 1080
Size: 323 73 684
Fragments arranged by size:
684 323 73
XmaI C'CCGG_G
Cuts at: 0 133 438 566 1080
Size: 133 305 128 514
Fragments arranged by size:
514 305 133 128
XmnI GAAnn'nnTTC
Cuts at: 0 645 757 1080
Size: 645 112 323
Fragments arranged by size:
645 323 112
Enzymes that do cut:
AcuI AleI AlfI ApaI AvaI BanI BanII BbeI
BbsI Bme1580I BpmI BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsmBI Bsp1286I BspEI BsrBI BsrFI
Bsu36I DraIII EaeI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI
NgoMIV PasI PpuMI PspOMI PstI PvuII RsrII SanDI
ScaI SfcI SfoI SmaI SmlI StuI StyI TaqII
TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BsaAI BsaBI BsiEI BsiHKAI BsiWI BsmI BspHI
BspMI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EagI EarI EciI EcoICRI EcoRI EcoRV
FseI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI SacI SacII SalI SapI
SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XcmI XhoI
ZraI