Restriction map for
pF1KE2487
(Linear) (Six-base) MAPSORT of: KIEE2487.seq Check: 6846 from: 1 to: 2052
KIEE2487.orf
With 182 enzymes: SgfI *
October 10, 2013 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1800 2052
Size: 1800 252
AccI GT'mk_AC
Cuts at: 0 1514 2052
Size: 1514 538
Acc65I G'GTAC_C
Cuts at: 0 23 2052
Size: 23 2029
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 676 2052
Size: 282 394 1376
Fragments arranged by size:
1376 394 282
AhdI GACnn_n'nnGTC
Cuts at: 0 936 2052
Size: 936 1116
ApoI r'AATT_y
Cuts at: 0 439 2052
Size: 439 1613
AvaI C'yCGr_G
Cuts at: 0 851 1355 2052
Size: 851 504 697
Fragments arranged by size:
851 697 504
BamHI G'GATC_C
Cuts at: 0 203 2052
Size: 203 1849
BanI G'GyrC_C
Cuts at: 0 23 432 2052
Size: 23 409 1620
Fragments arranged by size:
1620 409 23
BanII G_rGCy'C
Cuts at: 0 247 640 2052
Size: 247 393 1412
Fragments arranged by size:
1412 393 247
BbsI GAAGACnn'nnnn_
Cuts at: 0 251 374 606 1227 1839 2052
Size: 251 123 232 621 612 213
Fragments arranged by size:
621 612 251 232 213 123
BbvCI CC'TCA_GC
Cuts at: 0 773 2052
Size: 773 1279
BclI T'GATC_A
Cuts at: 0 504 1413 1538 2052
Size: 504 909 125 514
Fragments arranged by size:
909 514 504 125
BglI GCCn_nnn'nGGC
Cuts at: 0 1086 2052
Size: 1086 966
BlpI GC'TnA_GC
Cuts at: 0 424 1725 2052
Size: 424 1301 327
Fragments arranged by size:
1301 424 327
Bme1580I G_kGCm'C
Cuts at: 0 240 2052
Size: 240 1812
BmrI ACTGGGnnnn_n'
Cuts at: 0 1428 2052
Size: 1428 624
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2016 2052
Size: 2016 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1048 1194 2052
Size: 1048 146 858
Fragments arranged by size:
1048 858 146
Bpu10I CC'TnA_GC
Cuts at: 0 639 773 2001 2052
Size: 639 134 1228 51
Fragments arranged by size:
1228 639 134 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 1586 1876 1897 2052
Size: 553 1033 290 21 155
Fragments arranged by size:
1033 553 290 155 21
BsaI GGTCTCn'nnnn_
Cuts at: 0 1001 2052
Size: 1001 1051
BsaWI w'CCGG_w
Cuts at: 0 461 2052
Size: 461 1591
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1855 1885 2052
Size: 1855 30 167
Fragments arranged by size:
1855 167 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1 95 469 526 1138 2052
Size: 1 94 374 57 612 914
Fragments arranged by size:
914 612 374 94 57 1
BseYI C'CCAG_C
Cuts at: 0 362 721 2052
Size: 362 359 1331
Fragments arranged by size:
1331 362 359
Bsp1286I G_dGCh'C
Cuts at: 0 240 247 640 2052
Size: 240 7 393 1412
Fragments arranged by size:
1412 393 240 7
BspEI T'CCGG_A
Cuts at: 0 461 2052
Size: 461 1591
BspHI T'CATG_A
Cuts at: 0 1454 1559 2052
Size: 1454 105 493
Fragments arranged by size:
1454 493 105
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 606 871 1448 1559 1800 2052
Size: 606 265 577 111 241 252
Fragments arranged by size:
606 577 265 252 241 111
BsrDI GCAATG_nn'
Cuts at: 0 1347 2052
Size: 1347 705
BssSI C'ACGA_G
Cuts at: 0 1570 2052
Size: 1570 482
BstEII G'GTnAC_C
Cuts at: 0 1782 2052
Size: 1782 270
BstXI CCAn_nnnn'nTGG
Cuts at: 0 685 2052
Size: 685 1367
BstYI r'GATC_y
Cuts at: 0 203 467 2052
Size: 203 264 1585
Fragments arranged by size:
1585 264 203
BtgI C'CryG_G
Cuts at: 0 824 1464 2052
Size: 824 640 588
Fragments arranged by size:
824 640 588
BtsI GCAGTG_nn'
Cuts at: 0 1260 2052
Size: 1260 792
DraIII CAC_nnn'GTG
Cuts at: 0 1319 2052
Size: 1319 733
EaeI y'GGCC_r
Cuts at: 0 1943 2052
Size: 1943 109
EarI CTCTTCn'nnn_
Cuts at: 0 310 1962 2052
Size: 310 1652 90
Fragments arranged by size:
1652 310 90
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 169 921 2052
Size: 169 752 1131
Fragments arranged by size:
1131 752 169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 676 1048 1194 2052
Size: 282 394 372 146 858
Fragments arranged by size:
858 394 372 282 146
EcoNI CCTnn'n_nnAGG
Cuts at: 0 547 2052
Size: 547 1505
EcoO109I rG'GnC_Cy
Cuts at: 0 6 75 100 1032 1437 2010 2052
Size: 6 69 25 932 405 573 42
Fragments arranged by size:
932 573 405 69 42 25 6
EcoRV GAT'ATC
Cuts at: 0 589 2052
Size: 589 1463
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1842 2052
Size: 1842 210
HaeII r_GCGC'y
Cuts at: 0 728 2052
Size: 728 1324
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 747 779 922 954 1564 1584 1596 1616
Size: 747 32 143 32 610 20 12 20
Cuts at: 1616 2052
Size: 436
Fragments arranged by size:
747 610 436 143 32 32 20 20
12
KpnI G_GTAC'C
Cuts at: 0 27 2052
Size: 27 2025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 2052
Size: 49 2003
MscI TGG'CCA
Cuts at: 0 1945 2052
Size: 1945 107
MspA1I CmG'CkG
Cuts at: 0 522 1162 1172 1578 2052
Size: 522 640 10 406 474
Fragments arranged by size:
640 522 474 406 10
NcoI C'CATG_G
Cuts at: 0 1464 2052
Size: 1464 588
NspI r_CATG'y
Cuts at: 0 1510 2052
Size: 1510 542
PasI CC'CwG_GG
Cuts at: 0 631 1302 2052
Size: 631 671 750
Fragments arranged by size:
750 671 631
PflMI CCAn_nnn'nTGG
Cuts at: 0 141 2052
Size: 141 1911
PfoI T'CCnGG_A
Cuts at: 0 212 2052
Size: 212 1840
PpuMI rG'GwC_Cy
Cuts at: 0 6 1437 2052
Size: 6 1431 615
Fragments arranged by size:
1431 615 6
PshAI GACnn'nnGTC
Cuts at: 0 1529 1663 2052
Size: 1529 134 389
Fragments arranged by size:
1529 389 134
PstI C_TGCA'G
Cuts at: 0 1556 1706 1769 1811 2052
Size: 1556 150 63 42 241
Fragments arranged by size:
1556 241 150 63 42
PvuII CAG'CTG
Cuts at: 0 522 1162 1578 2052
Size: 522 640 416 474
Fragments arranged by size:
640 522 474 416
SbfI CC_TGCA'GG
Cuts at: 0 1769 2052
Size: 1769 283
SfcI C'TryA_G
Cuts at: 0 1552 1702 1765 1807 2052
Size: 1552 150 63 42 245
Fragments arranged by size:
1552 245 150 63 42
SmaI CCC'GGG
Cuts at: 0 1357 2052
Size: 1357 695
SmlI C'TyrA_G
Cuts at: 0 568 1601 1855 1912 2052
Size: 568 1033 254 57 140
Fragments arranged by size:
1033 568 254 140 57
StuI AGG'CCT
Cuts at: 0 653 2052
Size: 653 1399
StyI C'CwwG_G
Cuts at: 0 140 548 619 1464 2052
Size: 140 408 71 845 588
Fragments arranged by size:
845 588 408 140 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 926 2052
Size: 926 1126
Tth111I GACn'n_nGTC
Cuts at: 0 1710 2052
Size: 1710 342
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 739 2052
Size: 739 1313
XmaI C'CCGG_G
Cuts at: 0 1355 2052
Size: 1355 697
Enzymes that do cut:
AarI AccI Acc65I AcuI AhdI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BclI BglI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI
BseRI BseYI Bsp1286I BspEI BspHI BspMI BsrDI BssSI
BstEII BstXI BstYI BtgI BtsI DraIII EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I
KpnI MmeI MscI MspA1I NcoI NspI PasI PflMI
PfoI PpuMI PshAI PstI PvuII SbfI SfcI SmaI
SmlI StuI StyI TaqII Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BciVI BfrBI BglII BmgBI BmtI
BsaAI BsaBI BsaHI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EcoICRI
EcoRI FseI FspI FspAI HincII HindIII HpaI KasI
MfeI MluI MslI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI
TstI XbaI XhoI XmnI ZraI