Restriction map for
pF1KE2477
(Linear) (Six-base) MAPSORT of: KIEE2477.seq Check: 4145 from: 1 to: 1812
KIEE2477.orf
With 182 enzymes: SgfI *
June 18, 2013 11:58 ..
AatII G_ACGT'C
Cuts at: 0 263 689 1812
Size: 263 426 1123
Fragments arranged by size:
1123 426 263
AccI GT'mk_AC
Cuts at: 0 1736 1812
Size: 1736 76
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 1202 1812
Size: 157 1045 610
Fragments arranged by size:
1045 610 157
AflIII A'CryG_T
Cuts at: 0 989 1472 1628 1664 1812
Size: 989 483 156 36 148
Fragments arranged by size:
989 483 156 148 36
AlwNI CAG_nnn'CTG
Cuts at: 0 1275 1584 1812
Size: 1275 309 228
Fragments arranged by size:
1275 309 228
ApaLI G'TGCA_C
Cuts at: 0 751 1347 1812
Size: 751 596 465
Fragments arranged by size:
751 596 465
ApoI r'AATT_y
Cuts at: 0 516 1812
Size: 516 1296
AscI GG'CGCG_CC
Cuts at: 0 121 1812
Size: 121 1691
AvaI C'yCGr_G
Cuts at: 0 1480 1812
Size: 1480 332
BamHI G'GATC_C
Cuts at: 0 1511 1812
Size: 1511 301
BanI G'GyrC_C
Cuts at: 0 4 58 691 1812
Size: 4 54 633 1121
Fragments arranged by size:
1121 633 54 4
BanII G_rGCy'C
Cuts at: 0 1121 1812
Size: 1121 691
BbeI G_GCGC'C
Cuts at: 0 62 1812
Size: 62 1750
BbsI GAAGACnn'nnnn_
Cuts at: 0 536 845 1812
Size: 536 309 967
Fragments arranged by size:
967 536 309
BciVI GTATCCnnnnn_n'
Cuts at: 0 142 1812
Size: 142 1670
BglII A'GATC_T
Cuts at: 0 1094 1532 1812
Size: 1094 438 280
Fragments arranged by size:
1094 438 280
Bme1580I G_kGCm'C
Cuts at: 0 134 755 1351 1812
Size: 134 621 596 461
Fragments arranged by size:
621 596 461 134
BmgBI CAC'GTC
Cuts at: 0 1629 1812
Size: 1629 183
BmrI ACTGGGnnnn_n'
Cuts at: 0 1501 1812
Size: 1501 311
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1439 1812
Size: 1439 373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1216 1727 1812
Size: 1216 511 85
Fragments arranged by size:
1216 511 85
Bpu10I CC'TnA_GC
Cuts at: 0 1321 1812
Size: 1321 491
BsaI GGTCTCn'nnnn_
Cuts at: 0 578 710 1812
Size: 578 132 1102
Fragments arranged by size:
1102 578 132
BsaAI yAC'GTr
Cuts at: 0 990 1473 1812
Size: 990 483 339
Fragments arranged by size:
990 483 339
BsaHI Gr'CG_yC
Cuts at: 0 59 260 686 1812
Size: 59 201 426 1126
Fragments arranged by size:
1126 426 201 59
BsaWI w'CCGG_w
Cuts at: 0 344 1812
Size: 344 1468
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1273 1303 1738 1768 1812
Size: 1273 30 435 30 44
Fragments arranged by size:
1273 435 44 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 259 718 1149 1213 1812
Size: 259 459 431 64 599
Fragments arranged by size:
599 459 431 259 64
BseYI C'CCAG_C
Cuts at: 0 12 1812
Size: 12 1800
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1028 1812
Size: 1028 784
BsiHKAI G_wGCw'C
Cuts at: 0 755 1351 1812
Size: 755 596 461
Fragments arranged by size:
755 596 461
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1619 1812
Size: 1619 193
Bsp1286I G_dGCh'C
Cuts at: 0 134 755 1121 1351 1812
Size: 134 621 366 230 461
Fragments arranged by size:
621 461 366 230 134
BspHI T'CATG_A
Cuts at: 0 329 486 1812
Size: 329 157 1326
Fragments arranged by size:
1326 329 157
BsrBI CCG'CTC
Cuts at: 0 294 1812
Size: 294 1518
BsrDI GCAATG_nn'
Cuts at: 0 1028 1812
Size: 1028 784
BsrFI r'CCGG_y
Cuts at: 0 1504 1812
Size: 1504 308
BsrGI T'GTAC_A
Cuts at: 0 407 632 725 1812
Size: 407 225 93 1087
Fragments arranged by size:
1087 407 225 93
BssHII G'CGCG_C
Cuts at: 0 121 1812
Size: 121 1691
BssSI C'ACGA_G
Cuts at: 0 1297 1812
Size: 1297 515
BstEII G'GTnAC_C
Cuts at: 0 1371 1812
Size: 1371 441
BstYI r'GATC_y
Cuts at: 0 944 1094 1511 1532 1726 1812
Size: 944 150 417 21 194 86
Fragments arranged by size:
944 417 194 150 86 21
Bsu36I CC'TnA_GG
Cuts at: 0 1703 1812
Size: 1703 109
BtgI C'CryG_G
Cuts at: 0 225 419 465 545 1812
Size: 225 194 46 80 1267
Fragments arranged by size:
1267 225 194 80 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1233 1485 1812
Size: 1233 252 327
Fragments arranged by size:
1233 327 252
BtsI GCAGTG_nn'
Cuts at: 0 670 1812
Size: 670 1142
EaeI y'GGCC_r
Cuts at: 0 40 523 1812
Size: 40 483 1289
Fragments arranged by size:
1289 483 40
EarI CTCTTCn'nnn_
Cuts at: 0 364 1812
Size: 364 1448
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 853 1812
Size: 853 959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 1202 1216 1727 1812
Size: 157 1045 14 511 85
Fragments arranged by size:
1045 511 157 85 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1198 1812
Size: 1198 614
EcoO109I rG'GnC_Cy
Cuts at: 0 461 1812
Size: 461 1351
EcoRI G'AATT_C
Cuts at: 0 516 1812
Size: 516 1296
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 517 1812
Size: 517 1295
HaeII r_GCGC'y
Cuts at: 0 62 1305 1812
Size: 62 1243 507
Fragments arranged by size:
1243 507 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 140 172 461 493 1273 1305 1587 1619
Size: 140 32 289 32 780 32 282 32
Cuts at: 1619 1718 1738 1750 1770 1812
Size: 99 20 12 20 42
Fragments arranged by size:
780 289 282 140 99 42 32 32
32 32 20 20 12
HincII GTy'rAC
Cuts at: 0 1188 1812
Size: 1188 624
HindIII A'AGCT_T
Cuts at: 0 976 1812
Size: 976 836
KasI G'GCGC_C
Cuts at: 0 58 1812
Size: 58 1754
MluI A'CGCG_T
Cuts at: 0 1664 1812
Size: 1664 148
MscI TGG'CCA
Cuts at: 0 525 1812
Size: 525 1287
MslI CAynn'nnrTG
Cuts at: 0 328 613 1812
Size: 328 285 1199
Fragments arranged by size:
1199 328 285
NaeI GCC'GGC
Cuts at: 0 1506 1812
Size: 1506 306
NarI GG'CG_CC
Cuts at: 0 59 1812
Size: 59 1753
NgoMIV G'CCGG_C
Cuts at: 0 1504 1812
Size: 1504 308
NspI r_CATG'y
Cuts at: 0 644 900 1812
Size: 644 256 912
Fragments arranged by size:
912 644 256
PasI CC'CwG_GG
Cuts at: 0 1590 1812
Size: 1590 222
PmlI CAC'GTG
Cuts at: 0 990 1812
Size: 990 822
PshAI GACnn'nnGTC
Cuts at: 0 494 1812
Size: 494 1318
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 883 915 1812
Size: 883 32 897
Fragments arranged by size:
897 883 32
PstI C_TGCA'G
Cuts at: 0 215 1812
Size: 215 1597
SapI GCTCTTCn'nnn_
Cuts at: 0 364 1812
Size: 364 1448
ScaI AGT'ACT
Cuts at: 0 892 1812
Size: 892 920
SfcI C'TryA_G
Cuts at: 0 211 1812
Size: 211 1601
SfoI GGC'GCC
Cuts at: 0 60 1812
Size: 60 1752
SgrAI Cr'CCGG_yG
Cuts at: 0 1504 1812
Size: 1504 308
SmaI CCC'GGG
Cuts at: 0 1482 1812
Size: 1482 330
SphI G_CATG'C
Cuts at: 0 644 1812
Size: 644 1168
SspI AAT'ATT
Cuts at: 0 537 1812
Size: 537 1275
StyI C'CwwG_G
Cuts at: 0 93 915 1002 1784 1812
Size: 93 822 87 782 28
Fragments arranged by size:
822 782 93 87 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 93 1812
Size: 93 1719
TatI w'GTAC_w
Cuts at: 0 317 407 632 725 890 1812
Size: 317 90 225 93 165 922
Fragments arranged by size:
922 317 225 165 93 90
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 109 1812
Size: 109 1703
XmaI C'CCGG_G
Cuts at: 0 1480 1812
Size: 1480 332
XmnI GAAnn'nnTTC
Cuts at: 0 358 1812
Size: 358 1454
ZraI GAC'GTC
Cuts at: 0 261 687 1812
Size: 261 426 1125
Fragments arranged by size:
1125 426 261
Enzymes that do cut:
AatII AccI AcuI AflIII AlwNI ApaLI ApoI AscI
AvaI BamHI BanI BanII BbeI BbsI BciVI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstEII BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII KasI MluI MscI MslI NaeI NarI
NgoMIV NspI PasI PmlI PshAI PsrI PstI SapI
ScaI SfcI SfoI SgrAI SmaI SphI SspI StyI
TaqII TatI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI AseI AsiSI AvrII BaeI BbvCI
BcgI BclI BfrBI BglI BlpI BmtI BpuEI BsaBI
BsiEI BsiWI BsmI BspEI BspMI BstAPI BstBI BstXI
BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoRV FseI FspI FspAI HpaI KpnI MfeI MmeI
MspA1I NcoI NdeI NheI NotI NruI NsiI PacI
PciI PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI
PspXI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI SexAI SfiI SgfI SmlI SnaBI SpeI SrfI
StuI SwaI TstI Tth111I XbaI XhoI