Restriction map for
pF1KE2472
(Linear) (Six-base) MAPSORT of: KIEE2472.seq Check: 6649 from: 1 to: 4389
KIEE2472.orf
With 182 enzymes: SgfI *
June 18, 2013 11:58 ..
Acc65I G'GTAC_C
Cuts at: 0 2426 4389
Size: 2426 1963
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 842 1159 1352 1723 2105 2996 3194 4100
Size: 842 317 193 371 382 891 198 906
Cuts at: 4100 4266 4389
Size: 166 123
Fragments arranged by size:
906 891 842 382 371 317 198 193
166 123
AfeI AGC'GCT
Cuts at: 0 2594 4389
Size: 2594 1795
AflIII A'CryG_T
Cuts at: 0 2869 4389
Size: 2869 1520
AhdI GACnn_n'nnGTC
Cuts at: 0 2004 4389
Size: 2004 2385
AleI CACnn'nnGTG
Cuts at: 0 412 3058 4389
Size: 412 2646 1331
Fragments arranged by size:
2646 1331 412
AlwNI CAG_nnn'CTG
Cuts at: 0 2014 4389
Size: 2014 2375
ApoI r'AATT_y
Cuts at: 0 276 828 960 1334 2101 2145 2173 2569
Size: 276 552 132 374 767 44 28 396
Cuts at: 2569 2653 3766 3832 4389
Size: 84 1113 66 557
Fragments arranged by size:
1113 767 557 552 396 374 276 132
84 66 44 28
AseI AT'TA_AT
Cuts at: 0 1237 3386 4389
Size: 1237 2149 1003
Fragments arranged by size:
2149 1237 1003
AvaI C'yCGr_G
Cuts at: 0 85 1792 4389
Size: 85 1707 2597
Fragments arranged by size:
2597 1707 85
BamHI G'GATC_C
Cuts at: 0 4073 4389
Size: 4073 316
BanI G'GyrC_C
Cuts at: 0 42 2426 3568 3685 4389
Size: 42 2384 1142 117 704
Fragments arranged by size:
2384 1142 704 117 42
BanII G_rGCy'C
Cuts at: 0 952 1403 2112 2162 4389
Size: 952 451 709 50 2227
Fragments arranged by size:
2227 952 709 451 50
BbeI G_GCGC'C
Cuts at: 0 46 4389
Size: 46 4343
BbsI GAAGACnn'nnnn_
Cuts at: 0 902 3260 4389
Size: 902 2358 1129
Fragments arranged by size:
2358 1129 902
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 149 183 4389
Size: 149 34 4206
Fragments arranged by size:
4206 149 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3347 4389
Size: 3347 1042
BclI T'GATC_A
Cuts at: 0 362 1083 1691 4389
Size: 362 721 608 2698
Fragments arranged by size:
2698 721 608 362
BfrBI ATG'CAT
Cuts at: 0 152 1300 3889 4389
Size: 152 1148 2589 500
Fragments arranged by size:
2589 1148 500 152
BglI GCCn_nnn'nGGC
Cuts at: 0 1782 4389
Size: 1782 2607
BmrI ACTGGGnnnn_n'
Cuts at: 0 2010 2703 4023 4389
Size: 2010 693 1320 366
Fragments arranged by size:
2010 1320 693 366
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3624 3756 4389
Size: 3624 132 633
Fragments arranged by size:
3624 633 132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 202 1041 2682 4389
Size: 118 84 839 1641 1707
Fragments arranged by size:
1707 1641 839 118 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 1417 3195 4089 4389
Size: 127 1290 1778 894 300
Fragments arranged by size:
1778 1290 894 300 127
BsaI GGTCTCn'nnnn_
Cuts at: 0 3137 4389
Size: 3137 1252
BsaBI GATnn'nnATC
Cuts at: 0 3335 4389
Size: 3335 1054
BsaHI Gr'CG_yC
Cuts at: 0 43 4389
Size: 43 4346
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1038 3616 4389
Size: 1038 2578 773
Fragments arranged by size:
2578 1038 773
BseYI C'CCAG_C
Cuts at: 0 36 1883 4389
Size: 36 1847 2506
Fragments arranged by size:
2506 1847 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1625 1822 2512 2540 4389
Size: 1625 197 690 28 1849
Fragments arranged by size:
1849 1625 690 197 28
BsiHKAI G_wGCw'C
Cuts at: 0 845 2112 2162 3742 4389
Size: 845 1267 50 1580 647
Fragments arranged by size:
1580 1267 845 647 50
BsmI GAATG_Cn'
Cuts at: 0 152 1209 2238 3951 4019 4389
Size: 152 1057 1029 1713 68 370
Fragments arranged by size:
1713 1057 1029 370 152 68
Bsp1286I G_dGCh'C
Cuts at: 0 845 952 1403 2112 2162 3742 4389
Size: 845 107 451 709 50 1580 647
Fragments arranged by size:
1580 845 709 647 451 107 50
BspHI T'CATG_A
Cuts at: 0 567 1086 3828 4389
Size: 567 519 2742 561
Fragments arranged by size:
2742 567 561 519
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 151 746 3430 4389
Size: 151 595 2684 959
Fragments arranged by size:
2684 959 595 151
BsrBI CCG'CTC
Cuts at: 0 90 930 4389
Size: 90 840 3459
Fragments arranged by size:
3459 840 90
BsrDI GCAATG_nn'
Cuts at: 0 1922 2344 4389
Size: 1922 422 2045
Fragments arranged by size:
2045 1922 422
BsrFI r'CCGG_y
Cuts at: 0 1797 2236 3497 4389
Size: 1797 439 1261 892
Fragments arranged by size:
1797 1261 892 439
BstAPI GCAn_nnn'nTGC
Cuts at: 0 263 2125 3665 4389
Size: 263 1862 1540 724
Fragments arranged by size:
1862 1540 724 263
BstBI TT'CG_AA
Cuts at: 0 1599 4389
Size: 1599 2790
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1019 2740 2939 3520 4389
Size: 1019 1721 199 581 869
Fragments arranged by size:
1721 1019 869 581 199
BstYI r'GATC_y
Cuts at: 0 4073 4389
Size: 4073 316
BtgI C'CryG_G
Cuts at: 0 46 234 519 4389
Size: 46 188 285 3870
Fragments arranged by size:
3870 285 188 46
BtsI GCAGTG_nn'
Cuts at: 0 916 4151 4389
Size: 916 3235 238
Fragments arranged by size:
3235 916 238
DraI TTT'AAA
Cuts at: 0 3771 4389
Size: 3771 618
DrdI GACnn_nn'nnGTC
Cuts at: 0 1369 4389
Size: 1369 3020
EaeI y'GGCC_r
Cuts at: 0 2021 3045 4232 4389
Size: 2021 1024 1187 157
Fragments arranged by size:
2021 1187 1024 157
EarI CTCTTCn'nnn_
Cuts at: 0 1371 1742 1961 2135 2280 2700 2774 3896
Size: 1371 371 219 174 145 420 74 1122
Cuts at: 3896 4372 4389
Size: 476 17
Fragments arranged by size:
1371 1122 476 420 371 219 174 145
74 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 273 4389
Size: 273 4116
EcoICRI GAG'CTC
Cuts at: 0 2110 2160 4389
Size: 2110 50 2229
Fragments arranged by size:
2229 2110 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 202 842 1041 1159 1352 1723 2105
Size: 118 84 640 199 118 193 371 382
Cuts at: 2105 2682 2996 3194 4100 4266 4389
Size: 577 314 198 906 166 123
Fragments arranged by size:
906 640 577 382 371 314 199 198
193 166 123 118 118 84
EcoO109I rG'GnC_Cy
Cuts at: 0 705 2987 4389
Size: 705 2282 1402
Fragments arranged by size:
2282 1402 705
EcoRI G'AATT_C
Cuts at: 0 2569 4389
Size: 2569 1820
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1504 4389
Size: 1504 2885
HaeII r_GCGC'y
Cuts at: 0 46 79 348 2596 4165 4389
Size: 46 33 269 2248 1569 224
Fragments arranged by size:
2248 1569 269 224 46 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 652 684 1024 1056 1234 1266 1519 1551
Size: 652 32 340 32 178 32 253 32
Cuts at: 1551 1990 2022 2943 2975 3589 3621 4389
Size: 439 32 921 32 614 32 768
Fragments arranged by size:
921 768 652 614 439 340 253 178
32 32 32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 303 1602 1613 2121 3020 4389
Size: 303 1299 11 508 899 1369
Fragments arranged by size:
1369 1299 899 508 303 11
KasI G'GCGC_C
Cuts at: 0 42 4389
Size: 42 4347
KpnI G_GTAC'C
Cuts at: 0 2430 4389
Size: 2430 1959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2920 4389
Size: 2920 1469
MscI TGG'CCA
Cuts at: 0 2023 3047 4234 4389
Size: 2023 1024 1187 155
Fragments arranged by size:
2023 1187 1024 155
MslI CAynn'nnrTG
Cuts at: 0 412 1056 2247 2969 3031 3058 4058 4271
Size: 412 644 1191 722 62 27 1000 213
Cuts at: 4271 4389
Size: 118
Fragments arranged by size:
1191 1000 722 644 412 213 118 62
27
MspA1I CmG'CkG
Cuts at: 0 48 260 375 888 1428 1622 4389
Size: 48 212 115 513 540 194 2767
Fragments arranged by size:
2767 540 513 212 194 115 48
NaeI GCC'GGC
Cuts at: 0 2238 4389
Size: 2238 2151
NarI GG'CG_CC
Cuts at: 0 43 4389
Size: 43 4346
NcoI C'CATG_G
Cuts at: 0 234 4389
Size: 234 4155
NgoMIV G'CCGG_C
Cuts at: 0 2236 4389
Size: 2236 2153
NsiI A_TGCA'T
Cuts at: 0 154 1302 3891 4389
Size: 154 1148 2589 498
Fragments arranged by size:
2589 1148 498 154
NspI r_CATG'y
Cuts at: 0 213 2959 4389
Size: 213 2746 1430
Fragments arranged by size:
2746 1430 213
PflMI CCAn_nnn'nTGG
Cuts at: 0 2882 3062 3980 4389
Size: 2882 180 918 409
Fragments arranged by size:
2882 918 409 180
PfoI T'CCnGG_A
Cuts at: 0 95 4389
Size: 95 4294
PpuMI rG'GwC_Cy
Cuts at: 0 705 2987 4389
Size: 705 2282 1402
Fragments arranged by size:
2282 1402 705
PshAI GACnn'nnGTC
Cuts at: 0 2992 4389
Size: 2992 1397
PsiI TTA'TAA
Cuts at: 0 2330 4389
Size: 2330 2059
PstI C_TGCA'G
Cuts at: 0 380 675 4158 4389
Size: 380 295 3483 231
Fragments arranged by size:
3483 380 295 231
PvuII CAG'CTG
Cuts at: 0 888 1428 4389
Size: 888 540 2961
Fragments arranged by size:
2961 888 540
SacI G_AGCT'C
Cuts at: 0 2112 2162 4389
Size: 2112 50 2227
Fragments arranged by size:
2227 2112 50
SacII CC_GC'GG
Cuts at: 0 49 4389
Size: 49 4340
SanDI GG'GwC_CC
Cuts at: 0 2987 4389
Size: 2987 1402
SapI GCTCTTCn'nnn_
Cuts at: 0 1742 3896 4389
Size: 1742 2154 493
Fragments arranged by size:
2154 1742 493
ScaI AGT'ACT
Cuts at: 0 382 4389
Size: 382 4007
SfcI C'TryA_G
Cuts at: 0 376 671 1836 2517 3783 3794 4154 4389
Size: 376 295 1165 681 1266 11 360 235
Fragments arranged by size:
1266 1165 681 376 360 295 235 11
SfoI GGC'GCC
Cuts at: 0 44 4389
Size: 44 4345
SmlI C'TyrA_G
Cuts at: 0 142 1396 3210 4068 4372 4389
Size: 142 1254 1814 858 304 17
Fragments arranged by size:
1814 1254 858 304 142 17
SphI G_CATG'C
Cuts at: 0 2959 4389
Size: 2959 1430
SspI AAT'ATT
Cuts at: 0 119 1117 3153 4356 4389
Size: 119 998 2036 1203 33
Fragments arranged by size:
2036 1203 998 119 33
StyI C'CwwG_G
Cuts at: 0 234 1284 3615 4389
Size: 234 1050 2331 774
Fragments arranged by size:
2331 1050 774 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 532 861 3743 4389
Size: 532 329 2882 646
Fragments arranged by size:
2882 646 532 329
TatI w'GTAC_w
Cuts at: 0 380 1805 4209 4333 4389
Size: 380 1425 2404 124 56
Fragments arranged by size:
2404 1425 380 124 56
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2350 2382 2663 2695 3098 3130 4389
Size: 2350 32 281 32 403 32 1259
Fragments arranged by size:
2350 1259 403 281 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1591 4181 4389
Size: 1591 2590 208
Fragments arranged by size:
2590 1591 208
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1135 1880 4389
Size: 1135 745 2509
Fragments arranged by size:
2509 1135 745
XmnI GAAnn'nnTTC
Cuts at: 0 2054 4355 4389
Size: 2054 2301 34
Fragments arranged by size:
2301 2054 34
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AhdI AleI AlwNI ApoI
AseI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BmrI BplI BpmI BpuEI
BsaI BsaBI BsaHI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstBI
BstXI BstYI BtgI BtsI DraI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PflMI PfoI PpuMI
PshAI PsiI PstI PvuII SacI SacII SanDI SapI
ScaI SfcI SfoI SmlI SphI SspI StyI TaqII
TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AlfI AloI
ApaI ApaLI AscI AsiSI AvrII BaeI BbvCI BglII
BlpI Bme1580I BmgBI BmtI Bpu10I BsaAI BsaWI BsaXI
BsiEI BsiWI BsmBI BspEI BsrGI BssHII BssSI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EagI EcoNI
EcoRV FseI FspI FspAI HincII HpaI MfeI MluI
NdeI NheI NotI NruI PacI PasI PciI PmeI
PmlI PpiI PspOMI PspXI PsrI PvuI RsrII SalI
SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI StuI SwaI XbaI XhoI XmaI ZraI