Restriction map for
pF1KE2468
(Linear) (Six-base) MAPSORT of: KIEE2468.seq Check: 8385 from: 1 to: 1704
KIEE2468.orf
With 182 enzymes: SgfI *
April 17, 2013 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 219 1704
Size: 219 1485
AfeI AGC'GCT
Cuts at: 0 304 1704
Size: 304 1400
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1249 1704
Size: 1249 455
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 736 768 1704
Size: 736 32 936
Fragments arranged by size:
936 736 32
AlwNI CAG_nnn'CTG
Cuts at: 0 216 1704
Size: 216 1488
ApaI G_GGCC'C
Cuts at: 0 932 1704
Size: 932 772
ApaLI G'TGCA_C
Cuts at: 0 147 1383 1704
Size: 147 1236 321
Fragments arranged by size:
1236 321 147
AvaI C'yCGr_G
Cuts at: 0 995 1278 1704
Size: 995 283 426
Fragments arranged by size:
995 426 283
BamHI G'GATC_C
Cuts at: 0 1267 1704
Size: 1267 437
BanI G'GyrC_C
Cuts at: 0 444 658 1553 1704
Size: 444 214 895 151
Fragments arranged by size:
895 444 214 151
BanII G_rGCy'C
Cuts at: 0 932 1316 1704
Size: 932 384 388
Fragments arranged by size:
932 388 384
BbsI GAAGACnn'nnnn_
Cuts at: 0 596 1602 1704
Size: 596 1006 102
Fragments arranged by size:
1006 596 102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 720 754 871 905 1704
Size: 720 34 117 34 799
Fragments arranged by size:
799 720 117 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 366 499 1020 1075 1704
Size: 366 133 521 55 629
Fragments arranged by size:
629 521 366 133 55
BlpI GC'TnA_GC
Cuts at: 0 13 1704
Size: 13 1691
Bme1580I G_kGCm'C
Cuts at: 0 151 449 661 932 1220 1331 1387 1556
Size: 151 298 212 271 288 111 56 169
Cuts at: 1556 1704
Size: 148
Fragments arranged by size:
298 288 271 212 169 151 148 111
56
BmrI ACTGGGnnnn_n'
Cuts at: 0 1686 1704
Size: 1686 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 529 799 1704
Size: 207 322 270 905
Fragments arranged by size:
905 322 270 207
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 1543 1704
Size: 880 663 161
Fragments arranged by size:
880 663 161
BsaI GGTCTCn'nnnn_
Cuts at: 0 992 1704
Size: 992 712
BsaHI Gr'CG_yC
Cuts at: 0 389 1704
Size: 389 1315
BsaWI w'CCGG_w
Cuts at: 0 917 1704
Size: 917 787
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 331 925 1704
Size: 331 594 779
Fragments arranged by size:
779 594 331
BseYI C'CCAG_C
Cuts at: 0 375 924 1704
Size: 375 549 780
Fragments arranged by size:
780 549 375
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 347 1704
Size: 298 49 1357
Fragments arranged by size:
1357 298 49
BsiHKAI G_wGCw'C
Cuts at: 0 151 698 1387 1704
Size: 151 547 689 317
Fragments arranged by size:
689 547 317 151
Bsp1286I G_dGCh'C
Cuts at: 0 151 449 661 698 932 1220 1316 1331
Size: 151 298 212 37 234 288 96 15
Cuts at: 1331 1387 1556 1704
Size: 56 169 148
Fragments arranged by size:
298 288 234 212 169 151 148 96
56 37 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 219 320 1704
Size: 219 101 1384
Fragments arranged by size:
1384 219 101
BsrBI CCG'CTC
Cuts at: 0 63 600 1704
Size: 63 537 1104
Fragments arranged by size:
1104 537 63
BsrFI r'CCGG_y
Cuts at: 0 502 1704
Size: 502 1202
BsrGI T'GTAC_A
Cuts at: 0 1463 1704
Size: 1463 241
BssSI C'ACGA_G
Cuts at: 0 739 1704
Size: 739 965
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1326 1704
Size: 1326 378
BstEII G'GTnAC_C
Cuts at: 0 1200 1704
Size: 1200 504
BstXI CCAn_nnnn'nTGG
Cuts at: 0 783 1086 1704
Size: 783 303 618
Fragments arranged by size:
783 618 303
BstYI r'GATC_y
Cuts at: 0 1267 1704
Size: 1267 437
BtgI C'CryG_G
Cuts at: 0 290 431 1704
Size: 290 141 1273
Fragments arranged by size:
1273 290 141
BtsI GCAGTG_nn'
Cuts at: 0 1331 1704
Size: 1331 373
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1376 1411 1704
Size: 1376 35 293
Fragments arranged by size:
1376 293 35
DraI TTT'AAA
Cuts at: 0 843 1704
Size: 843 861
DraIII CAC_nnn'GTG
Cuts at: 0 1326 1382 1704
Size: 1326 56 322
Fragments arranged by size:
1326 322 56
EaeI y'GGCC_r
Cuts at: 0 134 287 434 644 1076 1088 1397 1517
Size: 134 153 147 210 432 12 309 120
Cuts at: 1517 1704
Size: 187
Fragments arranged by size:
432 309 210 187 153 147 134 120
12
EarI CTCTTCn'nnn_
Cuts at: 0 211 598 1704
Size: 211 387 1106
Fragments arranged by size:
1106 387 211
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1262 1704
Size: 1262 442
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 529 799 1704
Size: 207 322 270 905
Fragments arranged by size:
905 322 270 207
EcoO109I rG'GnC_Cy
Cuts at: 0 568 682 735 928 929 1704
Size: 568 114 53 193 1 775
Fragments arranged by size:
775 568 193 114 53 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1149 1704
Size: 1149 555
FseI GG_CCGG'CC
Cuts at: 0 506 1704
Size: 506 1198
FspI TGC'GCA
Cuts at: 0 334 1704
Size: 334 1370
HaeII r_GCGC'y
Cuts at: 0 13 306 1704
Size: 13 293 1398
Fragments arranged by size:
1398 293 13
HincII GTy'rAC
Cuts at: 0 1632 1704
Size: 1632 72
MscI TGG'CCA
Cuts at: 0 136 1078 1090 1399 1519 1704
Size: 136 942 12 309 120 185
Fragments arranged by size:
942 309 185 136 120 12
MslI CAynn'nnrTG
Cuts at: 0 88 130 259 436 442 784 1084 1704
Size: 88 42 129 177 6 342 300 620
Fragments arranged by size:
620 342 300 177 129 88 42 6
MspA1I CmG'CkG
Cuts at: 0 1461 1704
Size: 1461 243
NaeI GCC'GGC
Cuts at: 0 504 1704
Size: 504 1200
NgoMIV G'CCGG_C
Cuts at: 0 502 1704
Size: 502 1202
NspI r_CATG'y
Cuts at: 0 264 783 1704
Size: 264 519 921
Fragments arranged by size:
921 519 264
PasI CC'CwG_GG
Cuts at: 0 1220 1704
Size: 1220 484
PflMI CCAn_nnn'nTGG
Cuts at: 0 143 216 1704
Size: 143 73 1488
Fragments arranged by size:
1488 143 73
PpuMI rG'GwC_Cy
Cuts at: 0 682 735 1704
Size: 682 53 969
Fragments arranged by size:
969 682 53
PspOMI G'GGCC_C
Cuts at: 0 928 1704
Size: 928 776
PstI C_TGCA'G
Cuts at: 0 908 1704
Size: 908 796
SanDI GG'GwC_CC
Cuts at: 0 682 1704
Size: 682 1022
SfcI C'TryA_G
Cuts at: 0 904 1704
Size: 904 800
SmlI C'TyrA_G
Cuts at: 0 895 1558 1704
Size: 895 663 146
Fragments arranged by size:
895 663 146
StuI AGG'CCT
Cuts at: 0 1276 1704
Size: 1276 428
StyI C'CwwG_G
Cuts at: 0 533 1704
Size: 533 1171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 336 1026 1704
Size: 336 690 678
Fragments arranged by size:
690 678 336
TatI w'GTAC_w
Cuts at: 0 242 1463 1704
Size: 242 1221 241
Fragments arranged by size:
1221 242 241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 674 706 1704
Size: 674 32 998
Fragments arranged by size:
998 674 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 438 1656 1704
Size: 438 1218 48
Fragments arranged by size:
1218 438 48
Enzymes that do cut:
AarI AfeI AlfI AloI AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbsI BcgI BglI BlpI Bme1580I
BmrI BpmI BpuEI BsaI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI
BstAPI BstEII BstXI BstYI BtgI BtsI CspCI DraI
DraIII EaeI EarI EciI Eco57MI EcoO109I FalI FseI
FspI HaeII HincII MscI MslI MspA1I NaeI NgoMIV
NspI PasI PflMI PpuMI PspOMI PstI SanDI SfcI
SmlI StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BciVI BclI BfrBI BglII BmgBI BmtI
BplI Bpu10I BsaAI BsaBI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BssHII BstBI BstZ17I Bsu36I
BtgZI ClaI DrdI EagI EcoICRI EcoNI EcoRI EcoRV
FspAI Hin4I HindIII HpaI KasI KpnI MfeI MluI
MmeI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmaI XmnI ZraI