(Linear) (Six-base) MAPSORT of: KIEE2467.seq  Check: 8944  from: 1  to: 693



KIEE2467.orf

 With 182 enzymes: SgfI * 

                        March 29, 2013 15:36  ..

AccI GT'mk_AC

Cuts at:      0     281     693 
   Size:        281     412
 
ApoI r'AATT_y

Cuts at:      0      21     520     693 
   Size:         21     499     173

  Fragments arranged by size: 

                 499     173      21
 
BanII G_rGCy'C

Cuts at:      0     601     693 
   Size:        601      92
 
Bme1580I G_kGCm'C

Cuts at:      0     672     693 
   Size:        672      21
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      82     693 
   Size:         82     611
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     693 
   Size:        110     583
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     321     693 
   Size:        321     372
 
BsaAI yAC'GTr

Cuts at:      0     279     693 
   Size:        279     414
 
BsiEI CG_ry'CG

Cuts at:      0     676     693 
   Size:        676      17
 
Bsp1286I G_dGCh'C

Cuts at:      0     601     672     693 
   Size:        601      71      21

  Fragments arranged by size: 

                 601      71      21
 
BsrDI GCAATG_nn'

Cuts at:      0      84     693 
   Size:         84     609
 
BsrFI r'CCGG_y

Cuts at:      0     675     693 
   Size:        675      18
 
BstYI r'GATC_y

Cuts at:      0     411     693 
   Size:        411     282
 
Bsu36I CC'TnA_GG

Cuts at:      0     664     693 
   Size:        664      29
 
BtsI GCAGTG_nn'

Cuts at:      0     146     693 
   Size:        146     547
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     552     587     693 
   Size:        552      35     106

  Fragments arranged by size: 

                 552     106      35
 
EaeI y'GGCC_r

Cuts at:      0     423     673     693 
   Size:        423     250      20

  Fragments arranged by size: 

                 423     250      20
 
EagI C'GGCC_G

Cuts at:      0     673     693 
   Size:        673      20
 
EarI CTCTTCn'nnn_

Cuts at:      0      91     319     693 
   Size:         91     228     374

  Fragments arranged by size: 

                 374     228      91
 
HincII GTy'rAC

Cuts at:      0     357     561     693 
   Size:        357     204     132

  Fragments arranged by size: 

                 357     204     132
 
MscI TGG'CCA

Cuts at:      0     425     693 
   Size:        425     268
 
MslI CAynn'nnrTG

Cuts at:      0     652     693 
   Size:        652      41
 
NaeI GCC'GGC

Cuts at:      0     677     693 
   Size:        677      16
 
NgoMIV G'CCGG_C

Cuts at:      0     675     693 
   Size:        675      18
 
NspI r_CATG'y

Cuts at:      0     584     693 
   Size:        584     109
 
SfcI C'TryA_G

Cuts at:      0     302     693 
   Size:        302     391
 
SmlI C'TyrA_G

Cuts at:      0     125     693 
   Size:        125     568
 
SnaBI TAC'GTA

Cuts at:      0     279     693 
   Size:        279     414
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     121     693 
   Size:        121     572
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     477     693 
   Size:        477     216
 

 Enzymes that do cut:

     AccI     ApoI    BanII Bme1580I     BmrI    BpuEI     BsaI    BsaAI
    BsiEI Bsp1286I    BsrDI    BsrFI    BstYI   Bsu36I     BtsI    CspCI
     EaeI     EagI     EarI   HincII     MscI     MslI     NaeI   NgoMIV
     NspI     SfcI     SmlI    SnaBI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI
     ClaI     DraI   DraIII     DrdI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI