Restriction map for
pF1KE2464
(Linear) (Six-base) MAPSORT of: KIEE2464.seq Check: 3484 from: 1 to: 2289
KIEE2464.orf
With 182 enzymes: SgfI *
March 1, 2013 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1965 2289
Size: 1965 324
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 232 370 751 985 1127 1354 1417
Size: 71 161 138 381 234 142 227 63
Cuts at: 1417 1630 1813 2289
Size: 213 183 476
Fragments arranged by size:
476 381 234 227 213 183 161 142
138 71 63
AflIII A'CryG_T
Cuts at: 0 1071 2219 2289
Size: 1071 1148 70
Fragments arranged by size:
1148 1071 70
AlwNI CAG_nnn'CTG
Cuts at: 0 579 808 1395 2289
Size: 579 229 587 894
Fragments arranged by size:
894 587 579 229
AvaI C'yCGr_G
Cuts at: 0 1381 2289
Size: 1381 908
BanII G_rGCy'C
Cuts at: 0 764 1026 2289
Size: 764 262 1263
Fragments arranged by size:
1263 764 262
BbsI GAAGACnn'nnnn_
Cuts at: 0 1262 1462 2289
Size: 1262 200 827
Fragments arranged by size:
1262 827 200
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1039 1073 1695 1729 2289
Size: 1039 34 622 34 560
Fragments arranged by size:
1039 622 560 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 483 2289
Size: 483 1806
BglII A'GATC_T
Cuts at: 0 1212 2289
Size: 1212 1077
BlpI GC'TnA_GC
Cuts at: 0 580 1061 2053 2289
Size: 580 481 992 236
Fragments arranged by size:
992 580 481 236
Bme1580I G_kGCm'C
Cuts at: 0 1836 2289
Size: 1836 453
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 720 2289
Size: 720 1569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 286 307 931 1152 1258 1339 1756
Size: 88 198 21 624 221 106 81 417
Cuts at: 1756 1828 1840 1924 2170 2289
Size: 72 12 84 246 119
Fragments arranged by size:
624 417 246 221 198 119 106 88
84 81 72 21 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 1195 2062 2289
Size: 776 419 867 227
Fragments arranged by size:
867 776 419 227
BsaI GGTCTCn'nnnn_
Cuts at: 0 105 589 608 980 1163 2038 2289
Size: 105 484 19 372 183 875 251
Fragments arranged by size:
875 484 372 251 183 105 19
BsaAI yAC'GTr
Cuts at: 0 1074 2289
Size: 1074 1215
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 400 430 1987 2017 2244 2274 2289
Size: 400 30 1557 30 227 30 15
Fragments arranged by size:
1557 400 227 30 30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 304 379 607 1066 1149 1255 1888 1921
Size: 304 75 228 459 83 106 633 33
Cuts at: 1921 1983 2270 2289
Size: 62 287 19
Fragments arranged by size:
633 459 304 287 228 106 83 75
62 33 19
BseYI C'CCAG_C
Cuts at: 0 1487 1892 2048 2289
Size: 1487 405 156 241
Fragments arranged by size:
1487 405 241 156
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 2289
Size: 513 1776
BsiHKAI G_wGCw'C
Cuts at: 0 323 586 678 764 1997 2167 2289
Size: 323 263 92 86 1233 170 122
Fragments arranged by size:
1233 323 263 170 122 92 86
BsmI GAATG_Cn'
Cuts at: 0 201 1309 2289
Size: 201 1108 980
Fragments arranged by size:
1108 980 201
Bsp1286I G_dGCh'C
Cuts at: 0 323 586 678 764 1026 1836 1997 2167
Size: 323 263 92 86 262 810 161 170
Cuts at: 2167 2289
Size: 122
Fragments arranged by size:
810 323 263 262 170 161 122 92
86
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 462 535 1595 1709 1965 2289
Size: 462 73 1060 114 256 324
Fragments arranged by size:
1060 462 324 256 114 73
BsrBI CCG'CTC
Cuts at: 0 1665 2270 2289
Size: 1665 605 19
Fragments arranged by size:
1665 605 19
BsrDI GCAATG_nn'
Cuts at: 0 60 2289
Size: 60 2229
BsrFI r'CCGG_y
Cuts at: 0 2123 2289
Size: 2123 166
BssHII G'CGCG_C
Cuts at: 0 715 2289
Size: 715 1574
BssSI C'ACGA_G
Cuts at: 0 322 2289
Size: 322 1967
BstXI CCAn_nnnn'nTGG
Cuts at: 0 435 2289
Size: 435 1854
BstYI r'GATC_y
Cuts at: 0 1092 1212 1517 2289
Size: 1092 120 305 772
Fragments arranged by size:
1092 772 305 120
Bsu36I CC'TnA_GG
Cuts at: 0 622 2289
Size: 622 1667
BtgI C'CryG_G
Cuts at: 0 824 2289
Size: 824 1465
BtsI GCAGTG_nn'
Cuts at: 0 633 676 2289
Size: 633 43 1613
Fragments arranged by size:
1613 633 43
DrdI GACnn_nn'nnGTC
Cuts at: 0 1286 2289
Size: 1286 1003
EaeI y'GGCC_r
Cuts at: 0 251 2289
Size: 251 2038
EarI CTCTTCn'nnn_
Cuts at: 0 275 752 1039 2289
Size: 275 477 287 1250
Fragments arranged by size:
1250 477 287 275
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 42 457 709 2103 2289
Size: 42 415 252 1394 186
Fragments arranged by size:
1394 415 252 186 42
EcoICRI GAG'CTC
Cuts at: 0 762 2289
Size: 762 1527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 88 232 286 307 370 751 931
Size: 71 17 144 54 21 63 381 180
Cuts at: 931 985 1127 1152 1258 1339 1354 1417 1630
Size: 54 142 25 106 81 15 63 213
Cuts at: 1630 1756 1813 1828 1840 1924 2170 2289
Size: 126 57 15 12 84 246 119
Fragments arranged by size:
381 246 213 180 144 142 126 119
106 84 81 71 63 63 57 54
54 25 21 17 15 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 403 2289
Size: 403 1886
EcoO109I rG'GnC_Cy
Cuts at: 0 1928 2289
Size: 1928 361
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 781 1359 2289
Size: 781 578 930
Fragments arranged by size:
930 781 578
FspI TGC'GCA
Cuts at: 0 1705 2289
Size: 1705 584
HaeII r_GCGC'y
Cuts at: 0 246 2289
Size: 246 2043
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 127 159 171 203 891 923 2244 2276
Size: 127 32 12 32 688 32 1321 32
Cuts at: 2276 2289
Size: 13
Fragments arranged by size:
1321 688 127 32 32 32 32 13
12
HindIII A'AGCT_T
Cuts at: 0 1109 2289
Size: 1109 1180
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1925 2289
Size: 1925 364
MscI TGG'CCA
Cuts at: 0 253 2289
Size: 253 2036
MslI CAynn'nnrTG
Cuts at: 0 1920 2289
Size: 1920 369
MspA1I CmG'CkG
Cuts at: 0 920 963 1395 1578 1785 1896 2052 2082
Size: 920 43 432 183 207 111 156 30
Cuts at: 2082 2289
Size: 207
Fragments arranged by size:
920 432 207 207 183 156 111 43
30
NaeI GCC'GGC
Cuts at: 0 2125 2289
Size: 2125 164
NcoI C'CATG_G
Cuts at: 0 824 2289
Size: 824 1465
NgoMIV G'CCGG_C
Cuts at: 0 2123 2289
Size: 2123 166
NspI r_CATG'y
Cuts at: 0 897 2181 2289
Size: 897 1284 108
Fragments arranged by size:
1284 897 108
PflMI CCAn_nnn'nTGG
Cuts at: 0 306 2289
Size: 306 1983
PfoI T'CCnGG_A
Cuts at: 0 263 1129 2033 2289
Size: 263 866 904 256
Fragments arranged by size:
904 866 263 256
PmlI CAC'GTG
Cuts at: 0 1074 2289
Size: 1074 1215
PpuMI rG'GwC_Cy
Cuts at: 0 1928 2289
Size: 1928 361
PshAI GACnn'nnGTC
Cuts at: 0 146 2289
Size: 146 2143
PspXI vC'TCGA_Gb
Cuts at: 0 1381 2289
Size: 1381 908
PstI C_TGCA'G
Cuts at: 0 575 1592 1655 2289
Size: 575 1017 63 634
Fragments arranged by size:
1017 634 575 63
PvuII CAG'CTG
Cuts at: 0 963 1395 1578 1785 1896 2052 2082 2289
Size: 963 432 183 207 111 156 30 207
Fragments arranged by size:
963 432 207 207 183 156 111 30
RsrII CG'GwC_CG
Cuts at: 0 2106 2289
Size: 2106 183
SacI G_AGCT'C
Cuts at: 0 764 2289
Size: 764 1525
SanDI GG'GwC_CC
Cuts at: 0 1928 2289
Size: 1928 361
SapI GCTCTTCn'nnn_
Cuts at: 0 275 752 1039 2289
Size: 275 477 287 1250
Fragments arranged by size:
1250 477 287 275
SfcI C'TryA_G
Cuts at: 0 128 571 1588 1651 1852 2289
Size: 128 443 1017 63 201 437
Fragments arranged by size:
1017 443 437 201 128 63
SmlI C'TyrA_G
Cuts at: 0 791 1174 1381 2041 2289
Size: 791 383 207 660 248
Fragments arranged by size:
791 660 383 248 207
SphI G_CATG'C
Cuts at: 0 897 2181 2289
Size: 897 1284 108
Fragments arranged by size:
1284 897 108
StuI AGG'CCT
Cuts at: 0 1402 2289
Size: 1402 887
StyI C'CwwG_G
Cuts at: 0 824 1988 2006 2289
Size: 824 1164 18 283
Fragments arranged by size:
1164 824 283 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 851 1004 2289
Size: 851 153 1285
Fragments arranged by size:
1285 851 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 400 432 1987 2019 2289
Size: 400 32 1555 32 270
Fragments arranged by size:
1555 400 270 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 261 474 2289
Size: 261 213 1815
Fragments arranged by size:
1815 261 213
XhoI C'TCGA_G
Cuts at: 0 1381 2289
Size: 1381 908
XmnI GAAnn'nnTTC
Cuts at: 0 373 2289
Size: 373 1916
Enzymes that do cut:
AarI AcuI AflIII AlwNI AvaI BanII BbsI BcgI
BciVI BglII BlpI Bme1580I BplI BpmI BpuEI BsaI
BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstXI BstYI
Bsu36I BtgI BtsI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HindIII
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PflMI PfoI PmlI PpuMI PshAI PspXI PstI PvuII
RsrII SacI SanDI SapI SfcI SmlI SphI StuI
StyI TaqII TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BclI
BfrBI BglI BmgBI BmrI BmtI Bpu10I BsaBI BsaHI
BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrGI BstAPI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII
EagI EcoRI EcoRV FseI FspAI HincII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NsiI PacI PasI PciI PmeI PpiI PsiI PspOMI
PsrI PvuI SacII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI TatI Tth111I XbaI XmaI ZraI