(Linear) (Six-base) MAPSORT of: KIEE2462.seq  Check: 8848  from: 1  to: 354



KIEE2462.orf

 With 182 enzymes: SgfI * 

                        March 1, 2013 10:20  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     233     354 
   Size:        118     115     121

  Fragments arranged by size: 

                 121     118     115
 
AleI CACnn'nnGTG

Cuts at:      0     214     354 
   Size:        214     140
 
AlwNI CAG_nnn'CTG

Cuts at:      0      30      33     354 
   Size:         30       3     321

  Fragments arranged by size: 

                 321      30       3
 
ApoI r'AATT_y

Cuts at:      0     160     354 
   Size:        160     194
 
BamHI G'GATC_C

Cuts at:      0     190     354 
   Size:        190     164
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     354 
   Size:        235     119
 
Bpu10I CC'TnA_GC

Cuts at:      0     119     354 
   Size:        119     235
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     220     354 
   Size:        220     134
 
BsaBI GATnn'nnATC

Cuts at:      0     244     354 
   Size:        244     110
 
BsaWI w'CCGG_w

Cuts at:      0      89     354 
   Size:         89     265
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      33     354 
   Size:         33     321
 
BstYI r'GATC_y

Cuts at:      0     190     354 
   Size:        190     164
 
BtgI C'CryG_G

Cuts at:      0     195     354 
   Size:        195     159
 
EaeI y'GGCC_r

Cuts at:      0     319     354 
   Size:        319      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     233     235     354 
   Size:        118     115       2     119

  Fragments arranged by size: 

                 119     118     115       2
 
EcoRI G'AATT_C

Cuts at:      0     160     354 
   Size:        160     194
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      49      81     354 
   Size:         49      32     273

  Fragments arranged by size: 

                 273      49      32
 
MslI CAynn'nnrTG

Cuts at:      0     214     354 
   Size:        214     140
 
MspA1I CmG'CkG

Cuts at:      0      30     354 
   Size:         30     324
 
PstI C_TGCA'G

Cuts at:      0      38     149     354 
   Size:         38     111     205

  Fragments arranged by size: 

                 205     111      38
 
PvuII CAG'CTG

Cuts at:      0      30     354 
   Size:         30     324
 
SfcI C'TryA_G

Cuts at:      0      34     145     354 
   Size:         34     111     209

  Fragments arranged by size: 

                 209     111      34
 
StyI C'CwwG_G

Cuts at:      0     170     354 
   Size:        170     184
 
XmnI GAAnn'nnTTC

Cuts at:      0     160     354 
   Size:        160     194
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI     ApoI    BamHI     BpmI   Bpu10I     BsaI
    BsaBI    BsaWI   BstAPI    BstYI     BtgI     EaeI  Eco57MI    EcoRI
    Hin4I     MslI   MspA1I     PstI    PvuII     SfcI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaHI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI