Restriction map for
pF1KE2456
(Linear) (Six-base) MAPSORT of: KIEE2456.seq Check: 6217 from: 1 to: 5127
KIEE2456.orf
With 182 enzymes: SgfI *
March 1, 2013 10:20 ..
AccI GT'mk_AC
Cuts at: 0 3576 3712 4393 5127
Size: 3576 136 681 734
Fragments arranged by size:
3576 734 681 136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 518 1417 2659 2939 2960 3536 4061
Size: 65 453 899 1242 280 21 576 525
Cuts at: 4061 4136 4229 4238 5127
Size: 75 93 9 889
Fragments arranged by size:
1242 899 889 576 525 453 280 93
75 65 21 9
AgeI A'CCGG_T
Cuts at: 0 4928 5127
Size: 4928 199
AhdI GACnn_n'nnGTC
Cuts at: 0 4929 5127
Size: 4929 198
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3929 5127
Size: 3929 1198
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 770 802 5127
Size: 770 32 4325
Fragments arranged by size:
4325 770 32
AlwNI CAG_nnn'CTG
Cuts at: 0 88 190 496 538 754 1210 2854 5127
Size: 88 102 306 42 216 456 1644 2273
Fragments arranged by size:
2273 1644 456 306 216 102 88 42
ApoI r'AATT_y
Cuts at: 0 808 1643 1753 1781 1941 2314 3114 3197
Size: 808 835 110 28 160 373 800 83
Cuts at: 3197 3418 4459 4851 5127
Size: 221 1041 392 276
Fragments arranged by size:
1041 835 808 800 392 373 276 221
160 110 83 28
AseI AT'TA_AT
Cuts at: 0 2389 2612 3661 5127
Size: 2389 223 1049 1466
Fragments arranged by size:
2389 1466 1049 223
AvaI C'yCGr_G
Cuts at: 0 206 3356 3455 5127
Size: 206 3150 99 1672
Fragments arranged by size:
3150 1672 206 99
AvrII C'CTAG_G
Cuts at: 0 5023 5127
Size: 5023 104
BamHI G'GATC_C
Cuts at: 0 3834 5127
Size: 3834 1293
BanII G_rGCy'C
Cuts at: 0 1281 1958 2694 2960 5127
Size: 1281 677 736 266 2167
Fragments arranged by size:
2167 1281 736 677 266
BbsI GAAGACnn'nnnn_
Cuts at: 0 2839 3162 3345 4240 5127
Size: 2839 323 183 895 887
Fragments arranged by size:
2839 895 887 323 183
BbvCI CC'TCA_GC
Cuts at: 0 2209 2555 5127
Size: 2209 346 2572
Fragments arranged by size:
2572 2209 346
BclI T'GATC_A
Cuts at: 0 4533 4662 4824 4887 5127
Size: 4533 129 162 63 240
Fragments arranged by size:
4533 240 162 129 63
BfrBI ATG'CAT
Cuts at: 0 1307 5127
Size: 1307 3820
BglII A'GATC_T
Cuts at: 0 292 586 3945 4876 5044 5059 5127
Size: 292 294 3359 931 168 15 68
Fragments arranged by size:
3359 931 294 292 168 68 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 2837 3045 3511 3765 5127
Size: 2837 208 466 254 1362
Fragments arranged by size:
2837 1362 466 254 208
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 393 5127
Size: 393 4734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1085 2618 4151 5040 5127
Size: 1085 1533 1533 889 87
Fragments arranged by size:
1533 1533 1085 889 87
Bpu10I CC'TnA_GC
Cuts at: 0 2209 2555 5127
Size: 2209 346 2572
Fragments arranged by size:
2572 2209 346
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 997 2089 5127
Size: 289 708 1092 3038
Fragments arranged by size:
3038 1092 708 289
BsaI GGTCTCn'nnnn_
Cuts at: 0 4358 5127
Size: 4358 769
BsaAI yAC'GTr
Cuts at: 0 4560 5127
Size: 4560 567
BsaBI GATnn'nnATC
Cuts at: 0 168 468 2657 4763 5127
Size: 168 300 2189 2106 364
Fragments arranged by size:
2189 2106 364 300 168
BsaWI w'CCGG_w
Cuts at: 0 160 4928 5097 5127
Size: 160 4768 169 30
Fragments arranged by size:
4768 169 160 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 142 172 770 800 1914 1944 3252 3282
Size: 142 30 598 30 1114 30 1308 30
Cuts at: 3282 3497 3527 3608 3638 5127
Size: 215 30 81 30 1489
Fragments arranged by size:
1489 1308 1114 598 215 142 81 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 664 1922 4005 4058 5127
Size: 664 1258 2083 53 1069
Fragments arranged by size:
2083 1258 1069 664 53
BseYI C'CCAG_C
Cuts at: 0 1265 3815 5127
Size: 1265 2550 1312
Fragments arranged by size:
2550 1312 1265
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1281 3935 5127
Size: 1281 2654 1192
Fragments arranged by size:
2654 1281 1192
BsiHKAI G_wGCw'C
Cuts at: 0 1281 1958 5127
Size: 1281 677 3169
Fragments arranged by size:
3169 1281 677
BsmI GAATG_Cn'
Cuts at: 0 1674 1801 3633 5127
Size: 1674 127 1832 1494
Fragments arranged by size:
1832 1674 1494 127
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1918 5127
Size: 1918 3209
Bsp1286I G_dGCh'C
Cuts at: 0 1281 1958 2694 2960 5127
Size: 1281 677 736 266 2167
Fragments arranged by size:
2167 1281 736 677 266
BspEI T'CCGG_A
Cuts at: 0 160 5127
Size: 160 4967
BspHI T'CATG_A
Cuts at: 0 1572 1719 3003 5127
Size: 1572 147 1284 2124
Fragments arranged by size:
2124 1572 1284 147
BsrBI CCG'CTC
Cuts at: 0 1614 5127
Size: 1614 3513
BsrDI GCAATG_nn'
Cuts at: 0 2280 4028 4423 5127
Size: 2280 1748 395 704
Fragments arranged by size:
2280 1748 704 395
BsrFI r'CCGG_y
Cuts at: 0 4928 5127
Size: 4928 199
BstEII G'GTnAC_C
Cuts at: 0 901 5027 5127
Size: 901 4126 100
Fragments arranged by size:
4126 901 100
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2701 5127
Size: 2701 2426
BstYI r'GATC_y
Cuts at: 0 91 163 292 463 586 3289 3834 3945
Size: 91 72 129 171 123 2703 545 111
Cuts at: 3945 4138 4876 5044 5059 5127
Size: 193 738 168 15 68
Fragments arranged by size:
2703 738 545 193 171 168 129 123
111 91 72 68 15
BstZ17I GTA'TAC
Cuts at: 0 3713 4394 5127
Size: 3713 681 733
Fragments arranged by size:
3713 733 681
Bsu36I CC'TnA_GG
Cuts at: 0 797 2235 5127
Size: 797 1438 2892
Fragments arranged by size:
2892 1438 797
BtgI C'CryG_G
Cuts at: 0 3343 5127
Size: 3343 1784
BtsI GCAGTG_nn'
Cuts at: 0 153 390 5022 5127
Size: 153 237 4632 105
Fragments arranged by size:
4632 237 153 105
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 973 1008 5127
Size: 973 35 4119
Fragments arranged by size:
4119 973 35
DraI TTT'AAA
Cuts at: 0 1353 1466 1899 2906 4868 5127
Size: 1353 113 433 1007 1962 259
Fragments arranged by size:
1962 1353 1007 433 259 113
EaeI y'GGCC_r
Cuts at: 0 281 5127
Size: 281 4846
EarI CTCTTCn'nnn_
Cuts at: 0 362 497 797 2120 2882 2983 4214 5127
Size: 362 135 300 1323 762 101 1231 913
Fragments arranged by size:
1323 1231 913 762 362 300 135 101
EcoICRI GAG'CTC
Cuts at: 0 1279 1956 5127
Size: 1279 677 3171
Fragments arranged by size:
3171 1279 677
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 518 1085 1417 2618 2659 2939 2960
Size: 65 453 567 332 1201 41 280 21
Cuts at: 2960 3536 4061 4136 4151 4229 4238 5040 5127
Size: 576 525 75 15 78 9 802 87
Fragments arranged by size:
1201 802 576 567 525 453 332 280
87 78 75 65 41 21 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1635 2059 4304 5127
Size: 1635 424 2245 823
Fragments arranged by size:
2245 1635 823 424
EcoO109I rG'GnC_Cy
Cuts at: 0 1242 3019 5127
Size: 1242 1777 2108
Fragments arranged by size:
2108 1777 1242
EcoRI G'AATT_C
Cuts at: 0 1941 2314 5127
Size: 1941 373 2813
Fragments arranged by size:
2813 1941 373
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2782 3982 5127
Size: 2782 1200 1145
Fragments arranged by size:
2782 1200 1145
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 58 90 142 148 174 178 180 210
Size: 58 32 52 6 26 4 2 30
Cuts at: 210 284 316 388 420 478 510 1701 1719
Size: 74 32 72 32 58 32 1191 18
Cuts at: 1719 1733 1751 1885 1917 1945 1977 3292 3324
Size: 14 18 134 32 28 32 1315 32
Cuts at: 3324 3339 3371 4355 4387 4554 4586 4824 4831
Size: 15 32 984 32 167 32 238 7
Cuts at: 4831 4856 4863 4915 4947 5029 5061 5127
Size: 25 7 52 32 82 32 66
Fragments arranged by size:
1315 1191 984 238 167 134 82 74
72 66 58 58 52 52 32 32
32 32 32 32 32 32 32 32
32 32 30 28 26 25 18 18
15 14 7 7 6 4 2
HincII GTy'rAC
Cuts at: 0 1769 5127
Size: 1769 3358
HpaI GTT'AAC
Cuts at: 0 1769 5127
Size: 1769 3358
MfeI C'AATT_G
Cuts at: 0 3449 5127
Size: 3449 1678
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 820 3080 3622 5098 5127
Size: 38 782 2260 542 1476 29
Fragments arranged by size:
2260 1476 782 542 38 29
MslI CAynn'nnrTG
Cuts at: 0 14 434 2254 2549 3008 5127
Size: 14 420 1820 295 459 2119
Fragments arranged by size:
2119 1820 459 420 295 14
MspA1I CmG'CkG
Cuts at: 0 1210 1265 5127
Size: 1210 55 3862
Fragments arranged by size:
3862 1210 55
NsiI A_TGCA'T
Cuts at: 0 1309 5127
Size: 1309 3818
NspI r_CATG'y
Cuts at: 0 1307 4498 5127
Size: 1307 3191 629
Fragments arranged by size:
3191 1307 629
PacI TTA_AT'TAA
Cuts at: 0 959 5127
Size: 959 4168
PflMI CCAn_nnn'nTGG
Cuts at: 0 2967 5127
Size: 2967 2160
PfoI T'CCnGG_A
Cuts at: 0 4711 5127
Size: 4711 416
PmlI CAC'GTG
Cuts at: 0 4560 5127
Size: 4560 567
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 770 802 5127
Size: 770 32 4325
Fragments arranged by size:
4325 770 32
PpuMI rG'GwC_Cy
Cuts at: 0 3019 5127
Size: 3019 2108
PshAI GACnn'nnGTC
Cuts at: 0 3845 5127
Size: 3845 1282
PsiI TTA'TAA
Cuts at: 0 1055 1121 5127
Size: 1055 66 4006
Fragments arranged by size:
4006 1055 66
PvuII CAG'CTG
Cuts at: 0 1210 1265 5127
Size: 1210 55 3862
Fragments arranged by size:
3862 1210 55
SacI G_AGCT'C
Cuts at: 0 1281 1958 5127
Size: 1281 677 3169
Fragments arranged by size:
3169 1281 677
SapI GCTCTTCn'nnn_
Cuts at: 0 4214 5127
Size: 4214 913
ScaI AGT'ACT
Cuts at: 0 2840 5127
Size: 2840 2287
SexAI A'CCwGG_T
Cuts at: 0 3015 5127
Size: 3015 2112
SfcI C'TryA_G
Cuts at: 0 1936 3029 5127
Size: 1936 1093 2098
Fragments arranged by size:
2098 1936 1093
SmlI C'TyrA_G
Cuts at: 0 304 1012 2068 3455 5127
Size: 304 708 1056 1387 1672
Fragments arranged by size:
1672 1387 1056 708 304
SpeI A'CTAG_T
Cuts at: 0 3636 5127
Size: 3636 1491
SphI G_CATG'C
Cuts at: 0 1307 4498 5127
Size: 1307 3191 629
Fragments arranged by size:
3191 1307 629
SspI AAT'ATT
Cuts at: 0 1117 2173 2467 2724 3366 5127
Size: 1117 1056 294 257 642 1761
Fragments arranged by size:
1761 1117 1056 642 294 257
StuI AGG'CCT
Cuts at: 0 2335 4266 4299 4310 5127
Size: 2335 1931 33 11 817
Fragments arranged by size:
2335 1931 817 33 11
StyI C'CwwG_G
Cuts at: 0 1381 1527 1806 2123 2222 5023 5127
Size: 1381 146 279 317 99 2801 104
Fragments arranged by size:
2801 1381 317 279 146 104 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 4691 5127
Size: 4691 436
TatI w'GTAC_w
Cuts at: 0 2838 3618 5092 5127
Size: 2838 780 1474 35
Fragments arranged by size:
2838 1474 780 35
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1914 1946 3608 3640 4993 5025 5127
Size: 1914 32 1662 32 1353 32 102
Fragments arranged by size:
1914 1662 1353 102 32 32 32
XbaI T'CTAG_A
Cuts at: 0 2538 5127
Size: 2538 2589
XhoI C'TCGA_G
Cuts at: 0 3455 5127
Size: 3455 1672
XmnI GAAnn'nnTTC
Cuts at: 0 2762 3137 4196 4237 5127
Size: 2762 375 1059 41 890
Fragments arranged by size:
2762 1059 890 375 41
Enzymes that do cut:
AccI AcuI AgeI AhdI AlfI AloI AlwNI ApoI
AseI AvaI AvrII BamHI BanII BbsI BbvCI BclI
BfrBI BglII BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI CspCI
DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI Hin4I HincII HpaI MfeI MmeI MslI MspA1I
NsiI NspI PacI PflMI PfoI PmlI PpiI PpuMI
PshAI PsiI PvuII SacI SapI ScaI SexAI SfcI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI XbaI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AleI
ApaI ApaLI AscI AsiSI BaeI BanI BbeI BcgI
BciVI BglI BlpI Bme1580I BmgBI BmtI BsaHI BsiEI
BsiWI BspMI BsrGI BssHII BssSI BstAPI BstBI BtgZI
ClaI DraIII DrdI EagI EciI EcoRV FseI FspI
FspAI HaeII HindIII KasI KpnI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PasI
PciI PmeI PspOMI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI
SmaI SnaBI SrfI SwaI Tth111I XcmI XmaI ZraI