Restriction map for
pF1KE2454
(Linear) (Six-base) MAPSORT of: KIEE2454.seq Check: 1345 from: 1 to: 2538
KIEE2454.orf
With 182 enzymes: SgfI *
February 8, 2013 18:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1633 1789 1859 2538
Size: 1633 156 70 679
Fragments arranged by size:
1633 679 156 70
AflIII A'CryG_T
Cuts at: 0 1454 2538
Size: 1454 1084
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 220 2538
Size: 220 2318
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 925 957 1565 1597 2538
Size: 925 32 608 32 941
Fragments arranged by size:
941 925 608 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1756 2538
Size: 1756 782
ApaI G_GGCC'C
Cuts at: 0 528 2538
Size: 528 2010
ApaLI G'TGCA_C
Cuts at: 0 22 1777 2538
Size: 22 1755 761
Fragments arranged by size:
1755 761 22
ApoI r'AATT_y
Cuts at: 0 918 947 1030 2389 2413 2538
Size: 918 29 83 1359 24 125
Fragments arranged by size:
1359 918 125 83 29 24
AvaI C'yCGr_G
Cuts at: 0 98 527 548 581 2538
Size: 98 429 21 33 1957
Fragments arranged by size:
1957 429 98 33 21
BanI G'GyrC_C
Cuts at: 0 153 369 2538
Size: 153 216 2169
Fragments arranged by size:
2169 216 153
BanII G_rGCy'C
Cuts at: 0 19 528 1676 1710 2538
Size: 19 509 1148 34 828
Fragments arranged by size:
1148 828 509 34 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 740 1700 2538
Size: 740 960 838
Fragments arranged by size:
960 838 740
BbvCI CC'TCA_GC
Cuts at: 0 342 2538
Size: 342 2196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3 37 206 240 2538
Size: 3 34 169 34 2298
Fragments arranged by size:
2298 169 34 34 3
BciVI GTATCCnnnnn_n'
Cuts at: 0 918 2538
Size: 918 1620
BclI T'GATC_A
Cuts at: 0 1797 2538
Size: 1797 741
BglI GCCn_nnn'nGGC
Cuts at: 0 532 541 2538
Size: 532 9 1997
Fragments arranged by size:
1997 532 9
BlpI GC'TnA_GC
Cuts at: 0 410 2538
Size: 410 2128
Bme1580I G_kGCm'C
Cuts at: 0 26 528 1781 2455 2538
Size: 26 502 1253 674 83
Fragments arranged by size:
1253 674 502 83 26
BmrI ACTGGGnnnn_n'
Cuts at: 0 1893 2538
Size: 1893 645
Bpu10I CC'TnA_GC
Cuts at: 0 342 840 1340 2538
Size: 342 498 500 1198
Fragments arranged by size:
1198 500 498 342
BsaI GGTCTCn'nnnn_
Cuts at: 0 520 2538
Size: 520 2018
BsaAI yAC'GTr
Cuts at: 0 1197 2538
Size: 1197 1341
BsaWI w'CCGG_w
Cuts at: 0 134 2538
Size: 134 2404
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 498 528 1090 1120 2538
Size: 498 30 562 30 1418
Fragments arranged by size:
1418 562 498 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 84 157 330 415 516 519 568 1956
Size: 84 73 173 85 101 3 49 1388
Cuts at: 1956 2101 2538
Size: 145 437
Fragments arranged by size:
1388 437 173 145 101 85 84 73
49 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 2538
Size: 388 2150
BsiEI CG_ry'CG
Cuts at: 0 332 2538
Size: 332 2206
BsiHKAI G_wGCw'C
Cuts at: 0 26 1058 1710 1781 2538
Size: 26 1032 652 71 757
Fragments arranged by size:
1032 757 652 71 26
BsmI GAATG_Cn'
Cuts at: 0 1871 1952 2538
Size: 1871 81 586
Fragments arranged by size:
1871 586 81
BsmBI CGTCTCn'nnnn_
Cuts at: 0 63 2538
Size: 63 2475
Bsp1286I G_dGCh'C
Cuts at: 0 19 26 528 1058 1676 1710 1781 2455
Size: 19 7 502 530 618 34 71 674
Cuts at: 2455 2538
Size: 83
Fragments arranged by size:
674 618 530 502 83 71 34 19
7
BspEI T'CCGG_A
Cuts at: 0 134 2538
Size: 134 2404
BspHI T'CATG_A
Cuts at: 0 2115 2538
Size: 2115 423
BsrFI r'CCGG_y
Cuts at: 0 213 366 535 2538
Size: 213 153 169 2003
Fragments arranged by size:
2003 213 169 153
BsrGI T'GTAC_A
Cuts at: 0 1483 2538
Size: 1483 1055
BssSI C'ACGA_G
Cuts at: 0 992 2538
Size: 992 1546
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1066 2538
Size: 1066 1472
Bsu36I CC'TnA_GG
Cuts at: 0 479 2538
Size: 479 2059
BtgI C'CryG_G
Cuts at: 0 38 157 1492 2538
Size: 38 119 1335 1046
Fragments arranged by size:
1335 1046 119 38
BtsI GCAGTG_nn'
Cuts at: 0 1486 2538
Size: 1486 1052
DraI TTT'AAA
Cuts at: 0 1035 1478 2438 2538
Size: 1035 443 960 100
Fragments arranged by size:
1035 960 443 100
EaeI y'GGCC_r
Cuts at: 0 329 2538
Size: 329 2209
EagI C'GGCC_G
Cuts at: 0 329 2538
Size: 329 2209
EarI CTCTTCn'nnn_
Cuts at: 0 300 1139 1457 2538
Size: 300 839 318 1081
Fragments arranged by size:
1081 839 318 300
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 111 2538
Size: 19 92 2427
Fragments arranged by size:
2427 92 19
EcoICRI GAG'CTC
Cuts at: 0 1708 2538
Size: 1708 830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1633 1789 1859 2538
Size: 1633 156 70 679
Fragments arranged by size:
1633 679 156 70
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1067 2538
Size: 1067 1471
EcoO109I rG'GnC_Cy
Cuts at: 0 102 524 542 2538
Size: 102 422 18 1996
Fragments arranged by size:
1996 422 102 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 498 530 575 607 1692 1724 2128 2160
Size: 498 32 45 32 1085 32 404 32
Cuts at: 2160 2538
Size: 378
Fragments arranged by size:
1085 498 404 378 45 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1398 2538
Size: 1398 1140
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 998 1439 1740 2538
Size: 998 441 301 798
Fragments arranged by size:
998 798 441 301
MslI CAynn'nnrTG
Cuts at: 0 1491 2538
Size: 1491 1047
MspA1I CmG'CkG
Cuts at: 0 40 159 185 204 322 448 576 1753
Size: 40 119 26 19 118 126 128 1177
Cuts at: 1753 2038 2538
Size: 285 500
Fragments arranged by size:
1177 500 285 128 126 119 118 40
26 19
NaeI GCC'GGC
Cuts at: 0 215 537 2538
Size: 215 322 2001
Fragments arranged by size:
2001 322 215
NcoI C'CATG_G
Cuts at: 0 1492 2538
Size: 1492 1046
NgoMIV G'CCGG_C
Cuts at: 0 213 535 2538
Size: 213 322 2003
Fragments arranged by size:
2003 322 213
NspI r_CATG'y
Cuts at: 0 1458 2538
Size: 1458 1080
PciI A'CATG_T
Cuts at: 0 1454 2538
Size: 1454 1084
PflMI CCAn_nnn'nTGG
Cuts at: 0 1556 2538
Size: 1556 982
PfoI T'CCnGG_A
Cuts at: 0 1566 2538
Size: 1566 972
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1344 1376 2538
Size: 1344 32 1162
Fragments arranged by size:
1344 1162 32
PpuMI rG'GwC_Cy
Cuts at: 0 102 2538
Size: 102 2436
PspOMI G'GGCC_C
Cuts at: 0 524 2538
Size: 524 2014
PstI C_TGCA'G
Cuts at: 0 209 1188 2538
Size: 209 979 1350
Fragments arranged by size:
1350 979 209
PvuII CAG'CTG
Cuts at: 0 204 1753 2038 2538
Size: 204 1549 285 500
Fragments arranged by size:
1549 500 285 204
SacI G_AGCT'C
Cuts at: 0 1710 2538
Size: 1710 828
SacII CC_GC'GG
Cuts at: 0 41 160 2538
Size: 41 119 2378
Fragments arranged by size:
2378 119 41
SanDI GG'GwC_CC
Cuts at: 0 102 2538
Size: 102 2436
SfcI C'TryA_G
Cuts at: 0 205 1092 1184 1748 2324 2538
Size: 205 887 92 564 576 214
Fragments arranged by size:
887 576 564 214 205 92
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 532 541 2538
Size: 532 9 1997
Fragments arranged by size:
1997 532 9
SmaI CCC'GGG
Cuts at: 0 100 550 2538
Size: 100 450 1988
Fragments arranged by size:
1988 450 100
SspI AAT'ATT
Cuts at: 0 2310 2538
Size: 2310 228
StyI C'CwwG_G
Cuts at: 0 1492 2002 2201 2538
Size: 1492 510 199 337
Fragments arranged by size:
1492 510 337 199
TatI w'GTAC_w
Cuts at: 0 1483 2538
Size: 1483 1055
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 318 350 2434 2466 2538
Size: 318 32 2084 32 72
Fragments arranged by size:
2084 318 72 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1323 2538
Size: 1323 1215
XmaI C'CCGG_G
Cuts at: 0 98 548 2538
Size: 98 450 1990
Fragments arranged by size:
1990 450 98
XmnI GAAnn'nnTTC
Cuts at: 0 1356 1856 1952 2538
Size: 1356 500 96 586
Fragments arranged by size:
1356 586 500 96
Enzymes that do cut:
AcuI AflIII AlfI AloI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbsI BbvCI BcgI BciVI BclI
BglI BlpI Bme1580I BmrI Bpu10I BsaI BsaAI BsaWI
BsaXI BseRI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BsrFI BsrGI BssSI BstXI Bsu36I BtgI
BtsI DraI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I Hin4I HindIII MmeI MslI MspA1I NaeI
NcoI NgoMIV NspI PciI PflMI PfoI PpiI PpuMI
PspOMI PstI PvuII SacI SacII SanDI SfcI SfiI
SmaI SspI StyI TatI TstI Tth111I XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BfrBI BglII BmgBI BmtI BplI BpmI BpuEI
BsaBI BsaHI BseYI BsiWI BspMI BsrBI BsrDI BssHII
BstAPI BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI
DraIII DrdI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI MscI
NarI NdeI NheI NotI NruI NsiI PacI PasI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SapI SbfI ScaI SexAI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI StuI SwaI TaqII
XbaI XcmI XhoI ZraI