Restriction map for
pF1KE2450
(Linear) (Six-base) MAPSORT of: KIEE2450.seq Check: 1311 from: 1 to: 963
KIEE2450.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
Acc65I G'GTAC_C
Cuts at: 0 324 963
Size: 324 639
ApaLI G'TGCA_C
Cuts at: 0 640 963
Size: 640 323
AvaI C'yCGr_G
Cuts at: 0 499 736 963
Size: 499 237 227
Fragments arranged by size:
499 237 227
BanI G'GyrC_C
Cuts at: 0 324 579 963
Size: 324 255 384
Fragments arranged by size:
384 324 255
BanII G_rGCy'C
Cuts at: 0 104 963
Size: 104 859
BbeI G_GCGC'C
Cuts at: 0 583 963
Size: 583 380
BbsI GAAGACnn'nnnn_
Cuts at: 0 270 879 963
Size: 270 609 84
Fragments arranged by size:
609 270 84
BclI T'GATC_A
Cuts at: 0 359 963
Size: 359 604
BglI GCCn_nnn'nGGC
Cuts at: 0 635 963
Size: 635 328
Bme1580I G_kGCm'C
Cuts at: 0 644 963
Size: 644 319
BmrI ACTGGGnnnn_n'
Cuts at: 0 333 963
Size: 333 630
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 787 963
Size: 787 176
BsaHI Gr'CG_yC
Cuts at: 0 580 963
Size: 580 383
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 931 961 963
Size: 931 30 2
Fragments arranged by size:
931 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 84 784 963
Size: 84 700 179
Fragments arranged by size:
700 179 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 885 963
Size: 703 182 78
Fragments arranged by size:
703 182 78
BsiHKAI G_wGCw'C
Cuts at: 0 185 644 713 963
Size: 185 459 69 250
Fragments arranged by size:
459 250 185 69
Bsp1286I G_dGCh'C
Cuts at: 0 104 185 644 713 963
Size: 104 81 459 69 250
Fragments arranged by size:
459 250 104 81 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 173 614 963
Size: 173 441 349
Fragments arranged by size:
441 349 173
BsrDI GCAATG_nn'
Cuts at: 0 782 963
Size: 782 181
BsrGI T'GTAC_A
Cuts at: 0 611 963
Size: 611 352
BssSI C'ACGA_G
Cuts at: 0 289 963
Size: 289 674
BstAPI GCAn_nnn'nTGC
Cuts at: 0 689 717 963
Size: 689 28 246
Fragments arranged by size:
689 246 28
BstXI CCAn_nnnn'nTGG
Cuts at: 0 765 936 963
Size: 765 171 27
Fragments arranged by size:
765 171 27
BstYI r'GATC_y
Cuts at: 0 761 963
Size: 761 202
BtgI C'CryG_G
Cuts at: 0 575 624 963
Size: 575 49 339
Fragments arranged by size:
575 339 49
EaeI y'GGCC_r
Cuts at: 0 554 678 755 963
Size: 554 124 77 208
Fragments arranged by size:
554 208 124 77
EarI CTCTTCn'nnn_
Cuts at: 0 487 963
Size: 487 476
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 180 963
Size: 180 783
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 787 963
Size: 787 176
EcoNI CCTnn'n_nnAGG
Cuts at: 0 769 963
Size: 769 194
EcoO109I rG'GnC_Cy
Cuts at: 0 45 744 963
Size: 45 699 219
Fragments arranged by size:
699 219 45
EcoRV GAT'ATC
Cuts at: 0 15 963
Size: 15 948
HaeII r_GCGC'y
Cuts at: 0 546 575 583 963
Size: 546 29 8 380
Fragments arranged by size:
546 380 29 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 909 931 941 963
Size: 909 22 10 22
Fragments arranged by size:
909 22 22 10
KasI G'GCGC_C
Cuts at: 0 579 963
Size: 579 384
KpnI G_GTAC'C
Cuts at: 0 328 963
Size: 328 635
MscI TGG'CCA
Cuts at: 0 556 757 963
Size: 556 201 206
Fragments arranged by size:
556 206 201
MslI CAynn'nnrTG
Cuts at: 0 916 963
Size: 916 47
MspA1I CmG'CkG
Cuts at: 0 540 626 800 963
Size: 540 86 174 163
Fragments arranged by size:
540 174 163 86
NarI GG'CG_CC
Cuts at: 0 580 963
Size: 580 383
PasI CC'CwG_GG
Cuts at: 0 246 963
Size: 246 717
PfoI T'CCnGG_A
Cuts at: 0 764 963
Size: 764 199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 823 855 963
Size: 823 32 108
Fragments arranged by size:
823 108 32
PpuMI rG'GwC_Cy
Cuts at: 0 45 744 963
Size: 45 699 219
Fragments arranged by size:
699 219 45
PshAI GACnn'nnGTC
Cuts at: 0 451 963
Size: 451 512
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 595 627 963
Size: 595 32 336
Fragments arranged by size:
595 336 32
PstI C_TGCA'G
Cuts at: 0 225 404 550 829 963
Size: 225 179 146 279 134
Fragments arranged by size:
279 225 179 146 134
SacII CC_GC'GG
Cuts at: 0 627 963
Size: 627 336
SanDI GG'GwC_CC
Cuts at: 0 744 963
Size: 744 219
ScaI AGT'ACT
Cuts at: 0 343 963
Size: 343 620
SfcI C'TryA_G
Cuts at: 0 221 400 546 825 963
Size: 221 179 146 279 138
Fragments arranged by size:
279 221 179 146 138
SfoI GGC'GCC
Cuts at: 0 581 963
Size: 581 382
SmaI CCC'GGG
Cuts at: 0 501 963
Size: 501 462
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 873 963
Size: 873 90
TatI w'GTAC_w
Cuts at: 0 341 611 963
Size: 341 270 352
Fragments arranged by size:
352 341 270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 850 882 963
Size: 850 32 81
Fragments arranged by size:
850 81 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 363 963
Size: 363 600
XmaI C'CCGG_G
Cuts at: 0 499 963
Size: 499 464
Enzymes that do cut:
Acc65I ApaLI AvaI BanI BanII BbeI BbsI BclI
BglI Bme1580I BmrI BpmI BsaHI BsaXI BseRI BsgI
BsiHKAI Bsp1286I BspMI BsrDI BsrGI BssSI BstAPI BstXI
BstYI BtgI EaeI EarI EciI Eco57MI EcoNI EcoO109I
EcoRV HaeII Hin4I KasI KpnI MscI MslI MspA1I
NarI PasI PfoI PpiI PpuMI PshAI PsrI PstI
SacII SanDI ScaI SfcI SfoI SmaI TaqII TatI
TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrFI BssHII BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoRI FalI FseI FspI
FspAI HincII HindIII HpaI MfeI MluI MmeI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PsiI PspOMI PspXI
PvuI PvuII RsrII SacI SalI SapI SbfI SexAI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI Tth111I XbaI XhoI XmnI
ZraI