Restriction map for
pF1KE2446
(Linear) (Six-base) MAPSORT of: KIEE2446.seq Check: 2894 from: 1 to: 2145
KIEE2446.orf
With 182 enzymes: SgfI *
February 8, 2013 18:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 770 913 1195 1726 1941 2145
Size: 692 78 143 282 531 215 204
Fragments arranged by size:
692 531 282 215 204 143 78
AflIII A'CryG_T
Cuts at: 0 1649 1651 2145
Size: 1649 2 494
Fragments arranged by size:
1649 494 2
AlwNI CAG_nnn'CTG
Cuts at: 0 777 2145
Size: 777 1368
ApaI G_GGCC'C
Cuts at: 0 1182 2145
Size: 1182 963
ApoI r'AATT_y
Cuts at: 0 163 508 613 1275 1300 2145
Size: 163 345 105 662 25 845
Fragments arranged by size:
845 662 345 163 105 25
AvaI C'yCGr_G
Cuts at: 0 52 2145
Size: 52 2093
BamHI G'GATC_C
Cuts at: 0 537 2145
Size: 537 1608
BanI G'GyrC_C
Cuts at: 0 34 2114 2145
Size: 34 2080 31
Fragments arranged by size:
2080 34 31
BanII G_rGCy'C
Cuts at: 0 1182 1676 2145
Size: 1182 494 469
Fragments arranged by size:
1182 494 469
BbsI GAAGACnn'nnnn_
Cuts at: 0 1906 2145
Size: 1906 239
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1387 1421 2145
Size: 1387 34 724
Fragments arranged by size:
1387 724 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1818 2145
Size: 1818 327
BglI GCCn_nnn'nGGC
Cuts at: 0 1687 2145
Size: 1687 458
BglII A'GATC_T
Cuts at: 0 824 1743 2145
Size: 824 919 402
Fragments arranged by size:
919 824 402
Bme1580I G_kGCm'C
Cuts at: 0 1182 2145
Size: 1182 963
BmgBI CAC'GTC
Cuts at: 0 1377 2145
Size: 1377 768
BmrI ACTGGGnnnn_n'
Cuts at: 0 1031 2111 2145
Size: 1031 1080 34
Fragments arranged by size:
1080 1031 34
BmtI G_CTAG'C
Cuts at: 0 683 2145
Size: 683 1462
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1156 1252 1271 1510 1561 1789 2145
Size: 1156 96 19 239 51 228 356
Fragments arranged by size:
1156 356 239 228 96 51 19
Bpu10I CC'TnA_GC
Cuts at: 0 1844 2145
Size: 1844 301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1432 2145
Size: 1432 713
BsaI GGTCTCn'nnnn_
Cuts at: 0 587 1101 1754 2145
Size: 587 514 653 391
Fragments arranged by size:
653 587 514 391
BsaAI yAC'GTr
Cuts at: 0 1652 2145
Size: 1652 493
BsaWI w'CCGG_w
Cuts at: 0 1088 2145
Size: 1088 1057
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1138 1168 1954 1984 2145
Size: 1138 30 786 30 161
Fragments arranged by size:
1138 786 161 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 478 960 1507 1558 1561 2145
Size: 478 482 547 51 3 584
Fragments arranged by size:
584 547 482 478 51 3
BseYI C'CCAG_C
Cuts at: 0 30 1100 1535 2145
Size: 30 1070 435 610
Fragments arranged by size:
1070 610 435 30
BsiHKAI G_wGCw'C
Cuts at: 0 177 1676 2145
Size: 177 1499 469
Fragments arranged by size:
1499 469 177
Bsp1286I G_dGCh'C
Cuts at: 0 177 1182 1676 2145
Size: 177 1005 494 469
Fragments arranged by size:
1005 494 469 177
BspHI T'CATG_A
Cuts at: 0 695 2145
Size: 695 1450
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 605 785 846 2145
Size: 605 180 61 1299
Fragments arranged by size:
1299 605 180 61
BsrDI GCAATG_nn'
Cuts at: 0 1425 1442 2145
Size: 1425 17 703
Fragments arranged by size:
1425 703 17
BstEII G'GTnAC_C
Cuts at: 0 1884 2145
Size: 1884 261
BstYI r'GATC_y
Cuts at: 0 537 824 1743 2145
Size: 537 287 919 402
Fragments arranged by size:
919 537 402 287
Bsu36I CC'TnA_GG
Cuts at: 0 1000 2145
Size: 1000 1145
DraI TTT'AAA
Cuts at: 0 584 2145
Size: 584 1561
DrdI GACnn_nn'nnGTC
Cuts at: 0 1093 2145
Size: 1093 1052
EaeI y'GGCC_r
Cuts at: 0 849 1688 2145
Size: 849 839 457
Fragments arranged by size:
849 839 457
EarI CTCTTCn'nnn_
Cuts at: 0 638 746 909 913 1214 2012 2145
Size: 638 108 163 4 301 798 133
Fragments arranged by size:
798 638 301 163 133 108 4
EcoICRI GAG'CTC
Cuts at: 0 1674 2145
Size: 1674 471
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 770 913 1156 1195 1252 1271 1510
Size: 692 78 143 243 39 57 19 239
Cuts at: 1510 1561 1726 1789 1941 2145
Size: 51 165 63 152 204
Fragments arranged by size:
692 243 239 204 165 152 143 78
63 57 51 39 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 975 2145
Size: 975 1170
EcoO109I rG'GnC_Cy
Cuts at: 0 774 1178 2145
Size: 774 404 967
Fragments arranged by size:
967 774 404
EcoRI G'AATT_C
Cuts at: 0 613 2145
Size: 613 1532
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 823 2145
Size: 823 1322
FspI TGC'GCA
Cuts at: 0 601 2145
Size: 601 1544
HaeII r_GCGC'y
Cuts at: 0 52 2145
Size: 52 2093
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 184 216 547 579 677 709 1954 1986
Size: 184 32 331 32 98 32 1245 32
Cuts at: 1986 2145
Size: 159
Fragments arranged by size:
1245 331 184 159 98 32 32 32
32
HincII GTy'rAC
Cuts at: 0 717 2145
Size: 717 1428
HindIII A'AGCT_T
Cuts at: 0 586 2145
Size: 586 1559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 893 1181 1313 2145
Size: 893 288 132 832
Fragments arranged by size:
893 832 288 132
MscI TGG'CCA
Cuts at: 0 851 2145
Size: 851 1294
MspA1I CmG'CkG
Cuts at: 0 1221 1401 1539 1947 2145
Size: 1221 180 138 408 198
Fragments arranged by size:
1221 408 198 180 138
NheI G'CTAG_C
Cuts at: 0 679 2145
Size: 679 1466
NspI r_CATG'y
Cuts at: 0 1368 2145
Size: 1368 777
PflMI CCAn_nnn'nTGG
Cuts at: 0 666 2145
Size: 666 1479
PmlI CAC'GTG
Cuts at: 0 1652 2145
Size: 1652 493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 296 328 2145
Size: 296 32 1817
Fragments arranged by size:
1817 296 32
PpuMI rG'GwC_Cy
Cuts at: 0 774 2145
Size: 774 1371
PspOMI G'GGCC_C
Cuts at: 0 1178 2145
Size: 1178 967
PstI C_TGCA'G
Cuts at: 0 1637 2145
Size: 1637 508
PvuII CAG'CTG
Cuts at: 0 1221 1401 1539 1947 2145
Size: 1221 180 138 408 198
Fragments arranged by size:
1221 408 198 180 138
SacI G_AGCT'C
Cuts at: 0 1676 2145
Size: 1676 469
SapI GCTCTTCn'nnn_
Cuts at: 0 909 2012 2145
Size: 909 1103 133
Fragments arranged by size:
1103 909 133
ScaI AGT'ACT
Cuts at: 0 967 2145
Size: 967 1178
SfcI C'TryA_G
Cuts at: 0 788 1633 2145
Size: 788 845 512
Fragments arranged by size:
845 788 512
SmlI C'TyrA_G
Cuts at: 0 1411 2145
Size: 1411 734
StyI C'CwwG_G
Cuts at: 0 1568 2145
Size: 1568 577
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 22 2132 2145
Size: 22 2110 13
Fragments arranged by size:
2110 22 13
TatI w'GTAC_w
Cuts at: 0 745 965 2145
Size: 745 220 1180
Fragments arranged by size:
1180 745 220
Tth111I GACn'n_nGTC
Cuts at: 0 1023 2145
Size: 1023 1122
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1764 2145
Size: 1764 381
Enzymes that do cut:
AcuI AflIII AlwNI ApaI ApoI AvaI BamHI BanI
BanII BbsI BcgI BciVI BglI BglII Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI
BsaXI BseRI BseYI BsiHKAI Bsp1286I BspHI BspMI BsrDI
BstEII BstYI Bsu36I DraI DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII MmeI MscI MspA1I NheI NspI PflMI
PmlI PpiI PpuMI PspOMI PstI PvuII SacI SapI
ScaI SfcI SmlI StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BclI BfrBI BlpI BplI
BsaBI BsaHI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraIII EagI
EciI EcoRV FseI FspAI HpaI KasI KpnI MfeI
MluI MslI NaeI NarI NcoI NdeI NgoMIV NotI
NruI NsiI PacI PasI PciI PfoI PmeI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI XbaI
XhoI XmaI XmnI ZraI