Restriction map for
pF1KE2443
(Linear) (Six-base) MAPSORT of: KIEE2443.seq Check: 8317 from: 1 to: 963
KIEE2443.orf
With 182 enzymes: SgfI *
January 7, 2013 14:38 ..
AccI GT'mk_AC
Cuts at: 0 540 963
Size: 540 423
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 187 963
Size: 187 776
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 619 651 963
Size: 619 32 312
Fragments arranged by size:
619 312 32
ApaLI G'TGCA_C
Cuts at: 0 292 963
Size: 292 671
BamHI G'GATC_C
Cuts at: 0 71 101 963
Size: 71 30 862
Fragments arranged by size:
862 71 30
BanII G_rGCy'C
Cuts at: 0 861 963
Size: 861 102
BbsI GAAGACnn'nnnn_
Cuts at: 0 231 327 754 963
Size: 231 96 427 209
Fragments arranged by size:
427 231 209 96
Bme1580I G_kGCm'C
Cuts at: 0 296 963
Size: 296 667
BmrI ACTGGGnnnn_n'
Cuts at: 0 243 260 398 963
Size: 243 17 138 565
Fragments arranged by size:
565 243 138 17
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 963
Size: 401 562
Bpu10I CC'TnA_GC
Cuts at: 0 860 963
Size: 860 103
BsaI GGTCTCn'nnnn_
Cuts at: 0 347 963
Size: 347 616
BsaWI w'CCGG_w
Cuts at: 0 98 963
Size: 98 865
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 118 148 963
Size: 118 30 815
Fragments arranged by size:
815 118 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 963
Size: 64 899
BsiHKAI G_wGCw'C
Cuts at: 0 296 963
Size: 296 667
BsmBI CGTCTCn'nnnn_
Cuts at: 0 121 963
Size: 121 842
Bsp1286I G_dGCh'C
Cuts at: 0 296 861 963
Size: 296 565 102
Fragments arranged by size:
565 296 102
BspEI T'CCGG_A
Cuts at: 0 98 963
Size: 98 865
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 911 963
Size: 911 52
BstYI r'GATC_y
Cuts at: 0 71 101 963
Size: 71 30 862
Fragments arranged by size:
862 71 30
BtgI C'CryG_G
Cuts at: 0 38 963
Size: 38 925
BtsI GCAGTG_nn'
Cuts at: 0 408 545 963
Size: 408 137 418
Fragments arranged by size:
418 408 137
EaeI y'GGCC_r
Cuts at: 0 113 645 736 963
Size: 113 532 91 227
Fragments arranged by size:
532 227 113 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 291 963
Size: 291 672
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 963
Size: 401 562
EcoO109I rG'GnC_Cy
Cuts at: 0 376 900 963
Size: 376 524 63
Fragments arranged by size:
524 376 63
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 771 963
Size: 771 192
HincII GTy'rAC
Cuts at: 0 80 963
Size: 80 883
MscI TGG'CCA
Cuts at: 0 115 647 963
Size: 115 532 316
Fragments arranged by size:
532 316 115
MslI CAynn'nnrTG
Cuts at: 0 157 253 963
Size: 157 96 710
Fragments arranged by size:
710 157 96
MspA1I CmG'CkG
Cuts at: 0 40 963
Size: 40 923
PasI CC'CwG_GG
Cuts at: 0 676 963
Size: 676 287
PfoI T'CCnGG_A
Cuts at: 0 378 963
Size: 378 585
PpuMI rG'GwC_Cy
Cuts at: 0 376 900 963
Size: 376 524 63
Fragments arranged by size:
524 376 63
PstI C_TGCA'G
Cuts at: 0 176 464 963
Size: 176 288 499
Fragments arranged by size:
499 288 176
SacII CC_GC'GG
Cuts at: 0 41 963
Size: 41 922
SfcI C'TryA_G
Cuts at: 0 172 460 963
Size: 172 288 503
Fragments arranged by size:
503 288 172
SspI AAT'ATT
Cuts at: 0 652 963
Size: 652 311
StuI AGG'CCT
Cuts at: 0 193 963
Size: 193 770
StyI C'CwwG_G
Cuts at: 0 907 963
Size: 907 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 545 963
Size: 545 418
TatI w'GTAC_w
Cuts at: 0 795 963
Size: 795 168
Tth111I GACn'n_nGTC
Cuts at: 0 939 963
Size: 939 24
Enzymes that do cut:
AccI AlfI AloI ApaLI BamHI BanII BbsI Bme1580I
BmrI BpmI Bpu10I BsaI BsaWI BsaXI BsgI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BstYI BtgI BtsI EaeI
EciI Eco57MI EcoO109I FalI HincII MscI MslI MspA1I
PasI PfoI PpuMI PstI SacII SfcI SspI StuI
StyI TaqII TatI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlwNI ApaI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI
BplI BpuEI BsaAI BsaBI BsaHI BseRI BseYI BsiEI
BsiWI BsmI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII Hin4I
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SwaI TstI XbaI XcmI XhoI XmaI XmnI ZraI