(Linear) (Six-base) MAPSORT of: KIEE2439.seq  Check: 7005  from: 1  to: 429



KIEE2439.orf

 With 182 enzymes: SgfI * 

                        April 14, 2015 09:47  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     187     429 
   Size:        187     242
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     429 
   Size:        340      89
 
ApaI G_GGCC'C

Cuts at:      0     131     189     429 
   Size:        131      58     240

  Fragments arranged by size: 

                 240     131      58
 
AvaI C'yCGr_G

Cuts at:      0      96     429 
   Size:         96     333
 
BanI G'GyrC_C

Cuts at:      0     175     213     295     313     429 
   Size:        175      38      82      18     116

  Fragments arranged by size: 

                 175     116      82      38      18
 
BanII G_rGCy'C

Cuts at:      0     131     189     429 
   Size:        131      58     240

  Fragments arranged by size: 

                 240     131      58
 
BglI GCCn_nnn'nGGC

Cuts at:      0      43     429 
   Size:         43     386
 
Bme1580I G_kGCm'C

Cuts at:      0     131     178     189     218     292     429 
   Size:        131      47      11      29      74     137

  Fragments arranged by size: 

                 137     131      74      47      29      11
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     279     429 
   Size:        279     150
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      34     429 
   Size:         34     395
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     346     429 
   Size:        346      83
 
BsaWI w'CCGG_w

Cuts at:      0     203     429 
   Size:        203     226
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      10      13      22     429 
   Size:         10       3       9     407

  Fragments arranged by size: 

                 407      10       9       3
 
BseYI C'CCAG_C

Cuts at:      0     139     194     429 
   Size:        139      55     235

  Fragments arranged by size: 

                 235     139      55
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117     228     360     429 
   Size:        117     111     132      69

  Fragments arranged by size: 

                 132     117     111      69
 
BsiEI CG_ry'CG

Cuts at:      0      62     429 
   Size:         62     367
 
Bsp1286I G_dGCh'C

Cuts at:      0     131     178     189     218     292     429 
   Size:        131      47      11      29      74     137

  Fragments arranged by size: 

                 137     131      74      47      29      11
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     187     429 
   Size:        187     242
 
BssHII G'CGCG_C

Cuts at:      0     328     429 
   Size:        328     101
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     152     206     429 
   Size:        152      54     223

  Fragments arranged by size: 

                 223     152      54
 
BtgI C'CryG_G

Cuts at:      0      56     429 
   Size:         56     373
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     295     429 
   Size:        295     134
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     429 
   Size:        340      89
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     322     429 
   Size:        322     107
 
EcoO109I rG'GnC_Cy

Cuts at:      0     127     128     185     429 
   Size:        127       1      57     244

  Fragments arranged by size: 

                 244     127      57       1
 
HaeII r_GCGC'y

Cuts at:      0      49     429 
   Size:         49     380
 
MspA1I CmG'CkG

Cuts at:      0      58     429 
   Size:         58     371
 
PspOMI G'GGCC_C

Cuts at:      0     127     185     429 
   Size:        127      58     244

  Fragments arranged by size: 

                 244     127      58
 
SacII CC_GC'GG

Cuts at:      0      59     429 
   Size:         59     370
 
SmaI CCC'GGG

Cuts at:      0      98     429 
   Size:         98     331
 
StyI C'CwwG_G

Cuts at:      0     309     429 
   Size:        309     120
 
TatI w'GTAC_w

Cuts at:      0     254     429 
   Size:        254     175
 
XmaI C'CCGG_G

Cuts at:      0      96     429 
   Size:         96     333
 

 Enzymes that do cut:

     AarI     AcuI     ApaI     AvaI     BanI    BanII     BglI Bme1580I
     BmrI     BplI     BsaI    BsaWI    BseRI    BseYI     BsgI    BsiEI
 Bsp1286I    BspMI   BssHII   BstAPI     BtgI     EciI  Eco57MI    EcoNI
 EcoO109I    HaeII   MspA1I   PspOMI    SacII     SmaI     StyI     TatI
     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI