Restriction map for
pF1KE2438
(Linear) (Six-base) MAPSORT of: KIEE2438.seq Check: 7004 from: 1 to: 5823
KIEE2438.orf
With 182 enzymes: SgfI *
February 8, 2013 18:16 ..
AccI GT'mk_AC
Cuts at: 0 1301 5823
Size: 1301 4522
AclI AA'CG_TT
Cuts at: 0 324 5823
Size: 324 5499
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1910 2231 2717 3473 4531 4621 4795 5038
Size: 1910 321 486 756 1058 90 174 243
Cuts at: 5038 5344 5452 5614 5823
Size: 306 108 162 209
Fragments arranged by size:
1910 1058 756 486 321 306 243 209
174 162 108 90
AfeI AGC'GCT
Cuts at: 0 3424 5823
Size: 3424 2399
AflII C'TTAA_G
Cuts at: 0 838 5823
Size: 838 4985
AflIII A'CryG_T
Cuts at: 0 1484 4224 5823
Size: 1484 2740 1599
Fragments arranged by size:
2740 1599 1484
AleI CACnn'nnGTG
Cuts at: 0 4034 5823
Size: 4034 1789
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 314 3343 5823
Size: 314 3029 2480
Fragments arranged by size:
3029 2480 314
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1609 1641 3511 3543 5117 5149 5823
Size: 1609 32 1870 32 1574 32 674
Fragments arranged by size:
1870 1609 1574 674 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 604 1176 1347 1677 1960 2551 3036 3666
Size: 604 572 171 330 283 591 485 630
Cuts at: 3666 3822 4206 4278 5163 5460 5823
Size: 156 384 72 885 297 363
Fragments arranged by size:
885 630 604 591 572 485 384 363
330 297 283 171 156 72
ApoI r'AATT_y
Cuts at: 0 745 1096 1977 2287 2581 5701 5823
Size: 745 351 881 310 294 3120 122
Fragments arranged by size:
3120 881 745 351 310 294 122
AvaI C'yCGr_G
Cuts at: 0 409 3401 3437 5823
Size: 409 2992 36 2386
Fragments arranged by size:
2992 2386 409 36
BanI G'GyrC_C
Cuts at: 0 2062 3851 5823
Size: 2062 1789 1972
Fragments arranged by size:
2062 1972 1789
BanII G_rGCy'C
Cuts at: 0 128 1577 1881 1942 2333 5135 5823
Size: 128 1449 304 61 391 2802 688
Fragments arranged by size:
2802 1449 688 391 304 128 61
BbeI G_GCGC'C
Cuts at: 0 3855 5823
Size: 3855 1968
BbsI GAAGACnn'nnnn_
Cuts at: 0 193 222 559 2314 4013 5823
Size: 193 29 337 1755 1699 1810
Fragments arranged by size:
1810 1755 1699 337 193 29
BbvCI CC'TCA_GC
Cuts at: 0 2095 2795 4217 4928 5315 5519 5663 5672
Size: 2095 700 1422 711 387 204 144 9
Cuts at: 5672 5823
Size: 151
Fragments arranged by size:
2095 1422 711 700 387 204 151 144
9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5100 5134 5823
Size: 5100 34 689
Fragments arranged by size:
5100 689 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2467 4594 4965 5823
Size: 2467 2127 371 858
Fragments arranged by size:
2467 2127 858 371
BclI T'GATC_A
Cuts at: 0 222 527 981 3824 5186 5823
Size: 222 305 454 2843 1362 637
Fragments arranged by size:
2843 1362 637 454 305 222
BfrBI ATG'CAT
Cuts at: 0 1080 5823
Size: 1080 4743
BglI GCCn_nnn'nGGC
Cuts at: 0 3403 3481 3583 5287 5311 5823
Size: 3403 78 102 1704 24 512
Fragments arranged by size:
3403 1704 512 102 78 24
BglII A'GATC_T
Cuts at: 0 1188 5823
Size: 1188 4635
BlpI GC'TnA_GC
Cuts at: 0 1862 2819 2987 4412 5417 5823
Size: 1862 957 168 1425 1005 406
Fragments arranged by size:
1862 1425 1005 957 406 168
Bme1580I G_kGCm'C
Cuts at: 0 2297 5823
Size: 2297 3526
BmrI ACTGGGnnnn_n'
Cuts at: 0 2274 5823
Size: 2274 3549
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3414 5823
Size: 3414 2409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 709 1153 1447 1516 1810 2866 2998
Size: 554 155 444 294 69 294 1056 132
Cuts at: 2998 3391 3466 3571 3688 4195 4615 5227 5236
Size: 393 75 105 117 507 420 612 9
Cuts at: 5236 5374 5482 5698 5746 5823
Size: 138 108 216 48 77
Fragments arranged by size:
1056 612 554 507 444 420 393 294
294 216 155 138 132 117 108 105
77 75 69 48 9
Bpu10I CC'TnA_GC
Cuts at: 0 2095 2795 4217 4928 5315 5519 5663 5672
Size: 2095 700 1422 711 387 204 144 9
Cuts at: 5672 5823
Size: 151
Fragments arranged by size:
2095 1422 711 700 387 204 151 144
9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2851 2908 3202 4438 4726 4843 4951 5823
Size: 2851 57 294 1236 288 117 108 872
Fragments arranged by size:
2851 1236 872 294 288 117 108 57
BsaI GGTCTCn'nnnn_
Cuts at: 0 364 1053 1810 3735 4871 5823
Size: 364 689 757 1925 1136 952
Fragments arranged by size:
1925 1136 952 757 689 364
BsaHI Gr'CG_yC
Cuts at: 0 3852 4151 5823
Size: 3852 299 1672
Fragments arranged by size:
3852 1672 299
BsaWI w'CCGG_w
Cuts at: 0 5000 5823
Size: 5000 823
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 161 191 451 481 1420 1450 1609 1639
Size: 161 30 260 30 939 30 159 30
Cuts at: 1639 2040 2070 3511 3541 5117 5147 5823
Size: 401 30 1441 30 1576 30 676
Fragments arranged by size:
1576 1441 939 676 401 260 161 159
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 212 720 1132 1519 1630 2353 3070 3379
Size: 212 508 412 387 111 723 717 309
Cuts at: 3379 3388 3463 3568 3823 3994 4075 4078 4183
Size: 9 75 105 255 171 81 3 105
Cuts at: 4183 4192 5089 5121 5332 5680 5743 5823
Size: 9 897 32 211 348 63 80
Fragments arranged by size:
897 723 717 508 412 387 348 309
255 212 211 171 111 105 105 81
80 75 63 32 9 9 3
BseYI C'CCAG_C
Cuts at: 0 2363 4199 5823
Size: 2363 1836 1624
Fragments arranged by size:
2363 1836 1624
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 566 2412 2564 5149 5823
Size: 566 1846 152 2585 674
Fragments arranged by size:
2585 1846 674 566 152
BsiHKAI G_wGCw'C
Cuts at: 0 1287 1577 4098 4816 5135 5823
Size: 1287 290 2521 718 319 688
Fragments arranged by size:
2521 1287 718 688 319 290
BsmI GAATG_Cn'
Cuts at: 0 3320 4479 5823
Size: 3320 1159 1344
Fragments arranged by size:
3320 1344 1159
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4138 5823
Size: 4138 1685
Bsp1286I G_dGCh'C
Cuts at: 0 128 1287 1577 1881 1942 2297 2333 4098
Size: 128 1159 290 304 61 355 36 1765
Cuts at: 4098 4816 5135 5823
Size: 718 319 688
Fragments arranged by size:
1765 1159 718 688 355 319 304 290
128 61 36
BspEI T'CCGG_A
Cuts at: 0 5000 5823
Size: 5000 823
BspHI T'CATG_A
Cuts at: 0 2480 5823
Size: 2480 3343
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2956 3053 3653 4049 5063 5823
Size: 2956 97 600 396 1014 760
Fragments arranged by size:
2956 1014 760 600 396 97
BsrBI CCG'CTC
Cuts at: 0 3393 5358 5823
Size: 3393 1965 465
Fragments arranged by size:
3393 1965 465
BsrDI GCAATG_nn'
Cuts at: 0 1236 1391 1853 2981 5271 5750 5823
Size: 1236 155 462 1128 2290 479 73
Fragments arranged by size:
2290 1236 1128 479 462 155 73
BsrFI r'CCGG_y
Cuts at: 0 3475 5823
Size: 3475 2348
BsrGI T'GTAC_A
Cuts at: 0 308 5823
Size: 308 5515
BstAPI GCAn_nnn'nTGC
Cuts at: 0 595 1282 4014 4278 5823
Size: 595 687 2732 264 1545
Fragments arranged by size:
2732 1545 687 595 264
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4920 5823
Size: 4920 903
BstYI r'GATC_y
Cuts at: 0 1188 2473 4370 4688 4979 5391 5450 5823
Size: 1188 1285 1897 318 291 412 59 373
Fragments arranged by size:
1897 1285 1188 412 373 318 291 59
Bsu36I CC'TnA_GG
Cuts at: 0 1631 2002 3592 4936 5823
Size: 1631 371 1590 1344 887
Fragments arranged by size:
1631 1590 1344 887 371
BtgI C'CryG_G
Cuts at: 0 1820 2066 5823
Size: 1820 246 3757
Fragments arranged by size:
3757 1820 246
ClaI AT'CG_AT
Cuts at: 0 977 5823
Size: 977 4846
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1905 1940 5823
Size: 1905 35 3883
Fragments arranged by size:
3883 1905 35
DraIII CAC_nnn'GTG
Cuts at: 0 2025 5823
Size: 2025 3798
EaeI y'GGCC_r
Cuts at: 0 272 968 998 1289 2564 2900 3584 3617
Size: 272 696 30 291 1275 336 684 33
Cuts at: 3617 5823
Size: 2206
Fragments arranged by size:
2206 1275 696 684 336 291 272 33
30
EarI CTCTTCn'nnn_
Cuts at: 0 367 1607 1873 1947 2518 2533 2801 2830
Size: 367 1240 266 74 571 15 268 29
Cuts at: 2830 2951 3673 3800 3971 4673 4771 5779 5813
Size: 121 722 127 171 702 98 1008 34
Cuts at: 5813 5823
Size: 10
Fragments arranged by size:
1240 1008 722 702 571 367 268 266
171 127 121 98 74 34 29 15
10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 136 5823
Size: 136 5687
EcoICRI GAG'CTC
Cuts at: 0 1575 5133 5823
Size: 1575 3558 690
Fragments arranged by size:
3558 1575 690
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 709 1153 1447 1516 1810 1910 2231
Size: 554 155 444 294 69 294 100 321
Cuts at: 2231 2717 2866 2998 3391 3466 3473 3571 3688
Size: 486 149 132 393 75 7 98 117
Cuts at: 3688 4195 4531 4615 4621 4795 5038 5227 5236
Size: 507 336 84 6 174 243 189 9
Cuts at: 5236 5344 5374 5452 5482 5614 5698 5746 5823
Size: 108 30 78 30 132 84 48 77
Fragments arranged by size:
554 507 486 444 393 336 321 294
294 243 189 174 155 149 132 132
117 108 100 98 84 84 78 77
75 69 48 30 30 9 7 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 688 1612 3553 5059 5823
Size: 688 924 1941 1506 764
Fragments arranged by size:
1941 1506 924 764 688
EcoO109I rG'GnC_Cy
Cuts at: 0 89 450 1803 3125 3546 4296 4932 5013
Size: 89 361 1353 1322 421 750 636 81
Cuts at: 5013 5823
Size: 810
Fragments arranged by size:
1353 1322 810 750 636 421 361 89
81
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2338 2620 5823
Size: 2338 282 3203
Fragments arranged by size:
3203 2338 282
FspI TGC'GCA
Cuts at: 0 3541 3842 4696 5539 5823
Size: 3541 301 854 843 284
Fragments arranged by size:
3541 854 843 301 284
HaeII r_GCGC'y
Cuts at: 0 444 3426 3855 5348 5823
Size: 444 2982 429 1493 475
Fragments arranged by size:
2982 1493 475 444 429
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 270 302 502 534 715 747 840 872
Size: 270 32 200 32 181 32 93 32
Cuts at: 872 1998 2030 2040 2072 2514 2546 2952 2984
Size: 1126 32 10 32 442 32 406 32
Cuts at: 2984 4057 4089 5665 5697 5823
Size: 1073 32 1576 32 126
Fragments arranged by size:
1576 1126 1073 442 406 270 200 181
126 93 32 32 32 32 32 32
32 32 32 32 10
HincII GTy'rAC
Cuts at: 0 378 3069 5823
Size: 378 2691 2754
Fragments arranged by size:
2754 2691 378
KasI G'GCGC_C
Cuts at: 0 3851 5823
Size: 3851 1972
MfeI C'AATT_G
Cuts at: 0 593 3325 5823
Size: 593 2732 2498
Fragments arranged by size:
2732 2498 593
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1295 1806 5708 5823
Size: 1295 511 3902 115
Fragments arranged by size:
3902 1295 511 115
MscI TGG'CCA
Cuts at: 0 274 970 1000 1291 2566 2902 5823
Size: 274 696 30 291 1275 336 2921
Fragments arranged by size:
2921 1275 696 336 291 274 30
MslI CAynn'nnrTG
Cuts at: 0 290 1489 4034 4988 5823
Size: 290 1199 2545 954 835
Fragments arranged by size:
2545 1199 954 835 290
MspA1I CmG'CkG
Cuts at: 0 1347 1434 2094 2367 3583 3819 4203 4216
Size: 1347 87 660 273 1216 236 384 13
Cuts at: 4216 4275 4773 4902 5028 5424 5823
Size: 59 498 129 126 396 399
Fragments arranged by size:
1347 1216 660 498 399 396 384 273
236 129 126 87 59 13
NarI GG'CG_CC
Cuts at: 0 3852 5823
Size: 3852 1971
NcoI C'CATG_G
Cuts at: 0 2066 5823
Size: 2066 3757
NdeI CA'TA_TG
Cuts at: 0 930 5823
Size: 930 4893
NsiI A_TGCA'T
Cuts at: 0 1082 5823
Size: 1082 4741
NspI r_CATG'y
Cuts at: 0 1488 1626 2926 3607 4012 4228 4411 5823
Size: 1488 138 1300 681 405 216 183 1412
Fragments arranged by size:
1488 1412 1300 681 405 216 183 138
PasI CC'CwG_GG
Cuts at: 0 1214 5823
Size: 1214 4609
PciI A'CATG_T
Cuts at: 0 1484 4224 5823
Size: 1484 2740 1599
Fragments arranged by size:
2740 1599 1484
PflMI CCAn_nnn'nTGG
Cuts at: 0 1347 1677 2427 4593 5460 5823
Size: 1347 330 750 2166 867 363
Fragments arranged by size:
2166 1347 867 750 363 330
PfoI T'CCnGG_A
Cuts at: 0 1607 3014 5135 5252 5823
Size: 1607 1407 2121 117 571
Fragments arranged by size:
2121 1607 1407 571 117
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1297 1329 1519 1551 1609 1641 3511 3543
Size: 1297 32 190 32 58 32 1870 32
Cuts at: 3543 5117 5149 5823
Size: 1574 32 674
Fragments arranged by size:
1870 1574 1297 674 190 58 32 32
32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1803 3125 3546 4296 5013 5823
Size: 1803 1322 421 750 717 810
Fragments arranged by size:
1803 1322 810 750 717 421
PsiI TTA'TAA
Cuts at: 0 5651 5823
Size: 5651 172
PstI C_TGCA'G
Cuts at: 0 1188 3050 3650 3860 4019 4091 4211 4283
Size: 1188 1862 600 210 159 72 120 72
Cuts at: 4283 5063 5399 5823
Size: 780 336 424
Fragments arranged by size:
1862 1188 780 600 424 336 210 159
120 72 72
PvuII CAG'CTG
Cuts at: 0 1347 1434 2094 2367 4216 4773 4902 5028
Size: 1347 87 660 273 1849 557 129 126
Cuts at: 5028 5424 5823
Size: 396 399
Fragments arranged by size:
1849 1347 660 557 399 396 273 129
126 87
SacI G_AGCT'C
Cuts at: 0 1577 5135 5823
Size: 1577 3558 688
Fragments arranged by size:
3558 1577 688
SapI GCTCTTCn'nnn_
Cuts at: 0 1873 1947 5779 5823
Size: 1873 74 3832 44
Fragments arranged by size:
3832 1873 74 44
SbfI CC_TGCA'GG
Cuts at: 0 3050 5823
Size: 3050 2773
ScaI AGT'ACT
Cuts at: 0 583 1372 5823
Size: 583 789 4451
Fragments arranged by size:
4451 789 583
SfcI C'TryA_G
Cuts at: 0 1184 1256 2130 3046 3565 3646 3856 4015
Size: 1184 72 874 916 519 81 210 159
Cuts at: 4015 4087 4207 4279 4944 5059 5395 5823
Size: 72 120 72 665 115 336 428
Fragments arranged by size:
1184 916 874 665 519 428 336 210
159 120 115 81 72 72 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3403 5287 5823
Size: 3403 1884 536
Fragments arranged by size:
3403 1884 536
SfoI GGC'GCC
Cuts at: 0 3853 5823
Size: 3853 1970
SmaI CCC'GGG
Cuts at: 0 3403 3439 5823
Size: 3403 36 2384
Fragments arranged by size:
3403 2384 36
SmlI C'TyrA_G
Cuts at: 0 838 2866 2887 3181 4417 4705 4858 4966
Size: 838 2028 21 294 1236 288 153 108
Cuts at: 4966 5823
Size: 857
Fragments arranged by size:
2028 1236 857 838 294 288 153 108
21
SphI G_CATG'C
Cuts at: 0 3607 4012 4411 5823
Size: 3607 405 399 1412
Fragments arranged by size:
3607 1412 405 399
SspI AAT'ATT
Cuts at: 0 5598 5823
Size: 5598 225
StuI AGG'CCT
Cuts at: 0 694 2239 4423 5823
Size: 694 1545 2184 1400
Fragments arranged by size:
2184 1545 1400 694
StyI C'CwwG_G
Cuts at: 0 1706 2066 2300 2903 2996 3128 3740 4076
Size: 1706 360 234 603 93 132 612 336
Cuts at: 4076 4103 4446 4956 5823
Size: 27 343 510 867
Fragments arranged by size:
1706 867 612 603 510 360 343 336
234 132 93 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 521 2293 4969 5185 5823
Size: 521 1772 2676 216 638
Fragments arranged by size:
2676 1772 638 521 216
TatI w'GTAC_w
Cuts at: 0 308 581 1370 1508 2462 5823
Size: 308 273 789 138 954 3361
Fragments arranged by size:
3361 954 789 308 273 138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 161 193 451 483 1420 1452 1603 1635
Size: 161 32 258 32 937 32 151 32
Cuts at: 1635 5823
Size: 4188
Fragments arranged by size:
4188 937 258 161 151 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 1956 3064 5823
Size: 1956 1108 2759
Fragments arranged by size:
2759 1956 1108
XbaI T'CTAG_A
Cuts at: 0 820 3771 5823
Size: 820 2951 2052
Fragments arranged by size:
2951 2052 820
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 684 2287 3999 4191 5202 5349 5823
Size: 684 1603 1712 192 1011 147 474
Fragments arranged by size:
1712 1603 1011 684 474 192 147
XmaI C'CCGG_G
Cuts at: 0 3401 3437 5823
Size: 3401 36 2386
Fragments arranged by size:
3401 2386 36
XmnI GAAnn'nnTTC
Cuts at: 0 908 1312 2512 2582 5823
Size: 908 404 1200 70 3241
Fragments arranged by size:
3241 1200 908 404 70
Enzymes that do cut:
AccI AclI AcuI AfeI AflII AflIII AleI AlfI
AloI AlwNI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BstAPI BstXI BstYI Bsu36I BtgI ClaI CspCI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I HincII KasI MfeI MmeI MscI
MslI MspA1I NarI NcoI NdeI NsiI NspI PasI
PciI PflMI PfoI PpiI PpuMI PsiI PstI PvuII
SacI SapI SbfI ScaI SfcI SfiI SfoI SmaI
SmlI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AgeI AhdI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BmgBI BmtI BsaAI
BsaBI BsiEI BsiWI BssHII BssSI BstBI BstEII BstZ17I
BtgZI BtsI DraI DrdI EagI EcoRI EcoRV FseI
FspAI HindIII HpaI KpnI MluI NaeI NgoMIV NheI
NotI NruI PacI PmeI PmlI PshAI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SexAI SgfI
SgrAI SnaBI SpeI SrfI SwaI XhoI ZraI