(Linear) (Six-base) MAPSORT of: KIEE2435.seq  Check: 3359  from: 1  to: 999



KIEE2435.orf

 With 182 enzymes: SgfI * 

                        December 4, 2012 11:59  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     976     999 
   Size:        976      23
 
AleI CACnn'nnGTG

Cuts at:      0     514     999 
   Size:        514     485
 
AlwNI CAG_nnn'CTG

Cuts at:      0     147     999 
   Size:        147     852
 
ApoI r'AATT_y

Cuts at:      0     117     999 
   Size:        117     882
 
BanII G_rGCy'C

Cuts at:      0     945     999 
   Size:        945      54
 
BbvCI CC'TCA_GC

Cuts at:      0     337     999 
   Size:        337     662
 
BglII A'GATC_T

Cuts at:      0     416     561     999 
   Size:        416     145     438

  Fragments arranged by size: 

                 438     416     145
 
Bme1580I G_kGCm'C

Cuts at:      0     803     999 
   Size:        803     196
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     393     736     999 
   Size:         91     302     343     263

  Fragments arranged by size: 

                 343     302     263      91
 
Bpu10I CC'TnA_GC

Cuts at:      0     337     999 
   Size:        337     662
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     721     999 
   Size:        721     278
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     411     999 
   Size:        411     588
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     927     999 
   Size:        927      72
 
BseYI C'CCAG_C

Cuts at:      0     401     709     999 
   Size:        401     308     290

  Fragments arranged by size: 

                 401     308     290
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     444     999 
   Size:        444     555
 
Bsp1286I G_dGCh'C

Cuts at:      0     803     945     999 
   Size:        803     142      54

  Fragments arranged by size: 

                 803     142      54
 
BspHI T'CATG_A

Cuts at:      0     524     999 
   Size:        524     475
 
BsrDI GCAATG_nn'

Cuts at:      0     906     999 
   Size:        906      93
 
BsrFI r'CCGG_y

Cuts at:      0     188     999 
   Size:        188     811
 
BssSI C'ACGA_G

Cuts at:      0     701     999 
   Size:        701     298
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     207     999 
   Size:        207     792
 
BstYI r'GATC_y

Cuts at:      0     416     561     999 
   Size:        416     145     438

  Fragments arranged by size: 

                 438     416     145
 
BtsI GCAGTG_nn'

Cuts at:      0      86     999 
   Size:         86     913
 
EarI CTCTTCn'nnn_

Cuts at:      0     130     592     999 
   Size:        130     462     407

  Fragments arranged by size: 

                 462     407     130
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     393     736     976     999 
   Size:         91     302     343     240      23

  Fragments arranged by size: 

                 343     302     240      91      23
 
EcoRV GAT'ATC

Cuts at:      0     773     999 
   Size:        773     226
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      29     546     578     999 
   Size:         29     517      32     421

  Fragments arranged by size: 

                 517     421      32      29
 
HincII GTy'rAC

Cuts at:      0       5     999 
   Size:          5     994
 
MslI CAynn'nnrTG

Cuts at:      0     286     514     796     999 
   Size:        286     228     282     203

  Fragments arranged by size: 

                 286     282     228     203
 
NaeI GCC'GGC

Cuts at:      0     190     999 
   Size:        190     809
 
NgoMIV G'CCGG_C

Cuts at:      0     188     999 
   Size:        188     811
 
NspI r_CATG'y

Cuts at:      0     880     999 
   Size:        880     119
 
PasI CC'CwG_GG

Cuts at:      0     657     999 
   Size:        657     342
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     320     999 
   Size:        320     679
 
PfoI T'CCnGG_A

Cuts at:      0      68     409     985     999 
   Size:         68     341     576      14

  Fragments arranged by size: 

                 576     341      68      14
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     256     288     999 
   Size:        256      32     711

  Fragments arranged by size: 

                 711     256      32
 
SmlI C'TyrA_G

Cuts at:      0     736     999 
   Size:        736     263
 
SphI G_CATG'C

Cuts at:      0     880     999 
   Size:        880     119
 
TatI w'GTAC_w

Cuts at:      0      46     999 
   Size:         46     953
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI     ApoI    BanII    BbvCI    BglII Bme1580I
     BpmI   Bpu10I    BpuEI     BsaI    BseRI    BseYI     BsgI Bsp1286I
    BspHI    BsrDI    BsrFI    BssSI   BstAPI    BstYI     BtsI     EarI
  Eco57MI    EcoRV    Hin4I   HincII     MslI     NaeI   NgoMIV     NspI
     PasI    PflMI     PfoI     PsrI     SmlI     SphI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI
     BmtI     BplI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrGI
   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI