(Linear) (Six-base) MAPSORT of: KIEE2432.seq  Check: 7665  from: 1  to: 927



KIEE2432.orf

 With 182 enzymes: SgfI * 

                        October 12, 2018 16:45  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     287     927 
   Size:        287     640
 
ApoI r'AATT_y

Cuts at:      0      60      90     927 
   Size:         60      30     837

  Fragments arranged by size: 

                 837      60      30
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     771     927 
   Size:        771     156
 
BbvCI CC'TCA_GC

Cuts at:      0     304     927 
   Size:        304     623
 
BglII A'GATC_T

Cuts at:      0     119     927 
   Size:        119     808
 
Bme1580I G_kGCm'C

Cuts at:      0     560     927 
   Size:        560     367
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     906     927 
   Size:        906      21
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     927 
   Size:        144     783
 
Bpu10I CC'TnA_GC

Cuts at:      0     304     927 
   Size:        304     623
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     601     632     927 
   Size:        307     294      31     295

  Fragments arranged by size: 

                 307     295     294      31
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     162     927 
   Size:        162     765
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     381     519     927 
   Size:        381     138     408

  Fragments arranged by size: 

                 408     381     138
 
BseYI C'CCAG_C

Cuts at:      0     655     927 
   Size:        655     272
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     530     927 
   Size:        530     397
 
Bsp1286I G_dGCh'C

Cuts at:      0     560     927 
   Size:        560     367
 
BsrBI CCG'CTC

Cuts at:      0     102     927 
   Size:        102     825
 
BsrFI r'CCGG_y

Cuts at:      0     271     927 
   Size:        271     656
 
BstYI r'GATC_y

Cuts at:      0     119     643     651     927 
   Size:        119     524       8     276

  Fragments arranged by size: 

                 524     276     119       8
 
BtgI C'CryG_G

Cuts at:      0     633     927 
   Size:        633     294
 
BtsI GCAGTG_nn'

Cuts at:      0     143     518     927 
   Size:        143     375     409

  Fragments arranged by size: 

                 409     375     143
 
EaeI y'GGCC_r

Cuts at:      0     273     927 
   Size:        273     654
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     927 
   Size:        144     783
 
EcoO109I rG'GnC_Cy

Cuts at:      0     403     927 
   Size:        403     524
 
EcoRV GAT'ATC

Cuts at:      0     740     927 
   Size:        740     187
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     104     136     927 
   Size:        104      32     791

  Fragments arranged by size: 

                 791     104      32
 
HincII GTy'rAC

Cuts at:      0     427     927 
   Size:        427     500
 
HindIII A'AGCT_T

Cuts at:      0     677     927 
   Size:        677     250
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     365     927 
   Size:        365     562
 
MslI CAynn'nnrTG

Cuts at:      0     388     927 
   Size:        388     539
 
NcoI C'CATG_G

Cuts at:      0     633     927 
   Size:        633     294
 
PsiI TTA'TAA

Cuts at:      0     254     927 
   Size:        254     673
 
SmlI C'TyrA_G

Cuts at:      0     322     616     647     927 
   Size:        322     294      31     280

  Fragments arranged by size: 

                 322     294     280      31
 
StyI C'CwwG_G

Cuts at:      0     633     662     927 
   Size:        633      29     265

  Fragments arranged by size: 

                 633     265      29
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     839     888     927 
   Size:        839      49      39

  Fragments arranged by size: 

                 839      49      39
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     539     571     927 
   Size:        539      32     356

  Fragments arranged by size: 

                 539     356      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      36     927 
   Size:         36     891
 

 Enzymes that do cut:

    AlwNI     ApoI     BbsI    BbvCI    BglII Bme1580I     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BseRI    BseYI     BsgI Bsp1286I    BsrBI
    BsrFI    BstYI     BtgI     BtsI     EaeI  Eco57MI EcoO109I    EcoRV
    Hin4I   HincII  HindIII     MmeI     MslI     NcoI     PsiI     SmlI
     StyI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
    BmgBI     BmtI     BplI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI     FalI     FseI
     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI
     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI