Restriction map for
pF1KE2430
(Linear) (Six-base) MAPSORT of: KIEE2430.seq Check: 8487 from: 1 to: 1599
KIEE2430.orf
With 182 enzymes: SgfI *
January 7, 2013 14:38 ..
AatII G_ACGT'C
Cuts at: 0 179 1599
Size: 179 1420
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1030 1463 1599
Size: 718 312 433 136
Fragments arranged by size:
718 433 312 136
AflIII A'CryG_T
Cuts at: 0 1124 1599
Size: 1124 475
AleI CACnn'nnGTG
Cuts at: 0 1579 1599
Size: 1579 20
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 812 1599
Size: 812 787
AlwNI CAG_nnn'CTG
Cuts at: 0 267 1599
Size: 267 1332
ApaI G_GGCC'C
Cuts at: 0 1591 1599
Size: 1591 8
ApaLI G'TGCA_C
Cuts at: 0 270 1599
Size: 270 1329
AseI AT'TA_AT
Cuts at: 0 547 1599
Size: 547 1052
AvaI C'yCGr_G
Cuts at: 0 29 108 1599
Size: 29 79 1491
Fragments arranged by size:
1491 79 29
AvrII C'CTAG_G
Cuts at: 0 162 1599
Size: 162 1437
BanI G'GyrC_C
Cuts at: 0 96 112 437 1599
Size: 96 16 325 1162
Fragments arranged by size:
1162 325 96 16
BanII G_rGCy'C
Cuts at: 0 109 731 866 1040 1591 1599
Size: 109 622 135 174 551 8
Fragments arranged by size:
622 551 174 135 109 8
BbeI G_GCGC'C
Cuts at: 0 100 116 1599
Size: 100 16 1483
Fragments arranged by size:
1483 100 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 135 1599
Size: 135 1464
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 586 620 1599
Size: 586 34 979
Fragments arranged by size:
979 586 34
Bme1580I G_kGCm'C
Cuts at: 0 274 1591 1599
Size: 274 1317 8
Fragments arranged by size:
1317 274 8
BmgBI CAC'GTC
Cuts at: 0 1125 1599
Size: 1125 474
BmrI ACTGGGnnnn_n'
Cuts at: 0 1586 1599
Size: 1586 13
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1404 1599
Size: 1404 195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 1217 1599
Size: 697 520 382
Fragments arranged by size:
697 520 382
BsaBI GATnn'nnATC
Cuts at: 0 1430 1599
Size: 1430 169
BsaHI Gr'CG_yC
Cuts at: 0 97 113 176 974 1599
Size: 97 16 63 798 625
Fragments arranged by size:
798 625 97 63 16
BsaWI w'CCGG_w
Cuts at: 0 452 1599
Size: 452 1147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 1309 1599
Size: 46 1263 290
Fragments arranged by size:
1263 290 46
BseYI C'CCAG_C
Cuts at: 0 355 389 1599
Size: 355 34 1210
Fragments arranged by size:
1210 355 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1529 1599
Size: 1529 70
BsiEI CG_ry'CG
Cuts at: 0 130 230 1599
Size: 130 100 1369
Fragments arranged by size:
1369 130 100
BsiHKAI G_wGCw'C
Cuts at: 0 242 274 579 1040 1599
Size: 242 32 305 461 559
Fragments arranged by size:
559 461 305 242 32
BsmI GAATG_Cn'
Cuts at: 0 466 1599
Size: 466 1133
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1367 1599
Size: 1367 232
Bsp1286I G_dGCh'C
Cuts at: 0 109 242 274 579 731 866 1040 1591
Size: 109 133 32 305 152 135 174 551
Cuts at: 1591 1599
Size: 8
Fragments arranged by size:
551 305 174 152 135 133 109 32
8
BspHI T'CATG_A
Cuts at: 0 1070 1599
Size: 1070 529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 397 1599
Size: 397 1202
BsrFI r'CCGG_y
Cuts at: 0 125 229 1599
Size: 125 104 1370
Fragments arranged by size:
1370 125 104
BsrGI T'GTAC_A
Cuts at: 0 541 1599
Size: 541 1058
BstYI r'GATC_y
Cuts at: 0 311 1599
Size: 311 1288
Bsu36I CC'TnA_GG
Cuts at: 0 743 1599
Size: 743 856
BtgI C'CryG_G
Cuts at: 0 186 1599
Size: 186 1413
BtsI GCAGTG_nn'
Cuts at: 0 839 864 1599
Size: 839 25 735
Fragments arranged by size:
839 735 25
DraI TTT'AAA
Cuts at: 0 851 1599
Size: 851 748
DraIII CAC_nnn'GTG
Cuts at: 0 574 1599
Size: 574 1025
EaeI y'GGCC_r
Cuts at: 0 127 227 300 1079 1599
Size: 127 100 73 779 520
Fragments arranged by size:
779 520 127 100 73
EagI C'GGCC_G
Cuts at: 0 127 227 1599
Size: 127 100 1372
Fragments arranged by size:
1372 127 100
EarI CTCTTCn'nnn_
Cuts at: 0 1209 1254 1259 1599
Size: 1209 45 5 340
Fragments arranged by size:
1209 340 45 5
EcoICRI GAG'CTC
Cuts at: 0 1038 1599
Size: 1038 561
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 718 1030 1217 1463 1599
Size: 697 21 312 187 246 136
Fragments arranged by size:
697 312 246 187 136 21
EcoO109I rG'GnC_Cy
Cuts at: 0 34 738 1437 1551 1587 1588 1599
Size: 34 704 699 114 36 1 11
Fragments arranged by size:
704 699 114 36 34 11 1
EcoRV GAT'ATC
Cuts at: 0 502 1599
Size: 502 1097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 481 1599
Size: 481 1118
HaeII r_GCGC'y
Cuts at: 0 100 116 1599
Size: 100 16 1483
Fragments arranged by size:
1483 100 16
KasI G'GCGC_C
Cuts at: 0 96 112 1599
Size: 96 16 1487
Fragments arranged by size:
1487 96 16
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 408 493 1599
Size: 408 85 1106
Fragments arranged by size:
1106 408 85
MscI TGG'CCA
Cuts at: 0 1081 1599
Size: 1081 518
MslI CAynn'nnrTG
Cuts at: 0 794 1094 1579 1599
Size: 794 300 485 20
Fragments arranged by size:
794 485 300 20
MspA1I CmG'CkG
Cuts at: 0 75 267 291 359 1559 1599
Size: 75 192 24 68 1200 40
Fragments arranged by size:
1200 192 75 68 40 24
NaeI GCC'GGC
Cuts at: 0 127 231 1599
Size: 127 104 1368
Fragments arranged by size:
1368 127 104
NarI GG'CG_CC
Cuts at: 0 97 113 1599
Size: 97 16 1486
Fragments arranged by size:
1486 97 16
NdeI CA'TA_TG
Cuts at: 0 422 762 1599
Size: 422 340 837
Fragments arranged by size:
837 422 340
NgoMIV G'CCGG_C
Cuts at: 0 125 229 1599
Size: 125 104 1370
Fragments arranged by size:
1370 125 104
PflMI CCAn_nnn'nTGG
Cuts at: 0 1339 1599
Size: 1339 260
PfoI T'CCnGG_A
Cuts at: 0 242 1599
Size: 242 1357
PpuMI rG'GwC_Cy
Cuts at: 0 738 1437 1551 1599
Size: 738 699 114 48
Fragments arranged by size:
738 699 114 48
PspOMI G'GGCC_C
Cuts at: 0 1587 1599
Size: 1587 12
PstI C_TGCA'G
Cuts at: 0 846 1599
Size: 846 753
PvuII CAG'CTG
Cuts at: 0 291 359 1599
Size: 291 68 1240
Fragments arranged by size:
1240 291 68
SacI G_AGCT'C
Cuts at: 0 1040 1599
Size: 1040 559
SanDI GG'GwC_CC
Cuts at: 0 1437 1599
Size: 1437 162
SexAI A'CCwGG_T
Cuts at: 0 137 1599
Size: 137 1462
SfcI C'TryA_G
Cuts at: 0 656 842 893 1504 1599
Size: 656 186 51 611 95
Fragments arranged by size:
656 611 186 95 51
SfoI GGC'GCC
Cuts at: 0 98 114 1599
Size: 98 16 1485
Fragments arranged by size:
1485 98 16
SspI AAT'ATT
Cuts at: 0 1182 1264 1599
Size: 1182 82 335
Fragments arranged by size:
1182 335 82
StyI C'CwwG_G
Cuts at: 0 162 1568 1591 1599
Size: 162 1406 23 8
Fragments arranged by size:
1406 162 23 8
TatI w'GTAC_w
Cuts at: 0 541 1535 1599
Size: 541 994 64
Fragments arranged by size:
994 541 64
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 295 1224 1599
Size: 295 929 375
Fragments arranged by size:
929 375 295
XmnI GAAnn'nnTTC
Cuts at: 0 464 1599
Size: 464 1135
ZraI GAC'GTC
Cuts at: 0 177 1599
Size: 177 1422
Enzymes that do cut:
AatII AcuI AflIII AleI AlfI AlwNI ApaI ApaLI
AseI AvaI AvrII BanI BanII BbeI BbsI BcgI
Bme1580I BmgBI BmrI BplI BpmI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrFI BsrGI BstYI Bsu36I BtgI BtsI
DraI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoO109I
EcoRV FalI HaeII KasI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV PflMI PfoI PpuMI PspOMI
PstI PvuII SacI SanDI SexAI SfcI SfoI SspI
StyI TatI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApoI AscI AsiSI BaeI BamHI BbvCI BciVI
BclI BfrBI BglI BglII BlpI BmtI Bpu10I BpuEI
BsaI BsaAI BsaXI BsiWI BspEI BsrBI BsrDI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI
CspCI DrdI EciI EcoNI EcoRI FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI NcoI
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SapI SbfI ScaI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
SwaI TaqII TstI Tth111I XbaI XhoI XmaI