Restriction map for
pF1KE2429
(Linear) (Six-base) MAPSORT of: KIEE2429.seq Check: 7998 from: 1 to: 1974
KIEE2429.orf
With 182 enzymes: SgfI *
December 4, 2012 11:59 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 838 1387 1567 1974
Size: 508 330 549 180 407
Fragments arranged by size:
549 508 407 330 180
AflIII A'CryG_T
Cuts at: 0 1937 1974
Size: 1937 37
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 133 314 1974
Size: 133 181 1660
Fragments arranged by size:
1660 181 133
AlwNI CAG_nnn'CTG
Cuts at: 0 102 915 1974
Size: 102 813 1059
Fragments arranged by size:
1059 813 102
ApaI G_GGCC'C
Cuts at: 0 40 92 1974
Size: 40 52 1882
Fragments arranged by size:
1882 52 40
ApaLI G'TGCA_C
Cuts at: 0 197 1974
Size: 197 1777
ApoI r'AATT_y
Cuts at: 0 747 1360 1974
Size: 747 613 614
Fragments arranged by size:
747 614 613
AvaI C'yCGr_G
Cuts at: 0 32 368 449 850 1898 1974
Size: 32 336 81 401 1048 76
Fragments arranged by size:
1048 401 336 81 76 32
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 180 213 1974
Size: 180 33 1761
Fragments arranged by size:
1761 180 33
BanI G'GyrC_C
Cuts at: 0 3 1974
Size: 3 1971
BanII G_rGCy'C
Cuts at: 0 40 60 92 796 905 1387 1559 1718
Size: 40 20 32 704 109 482 172 159
Cuts at: 1718 1974
Size: 256
Fragments arranged by size:
704 482 256 172 159 109 40 32
20
BbsI GAAGACnn'nnnn_
Cuts at: 0 179 496 1940 1974
Size: 179 317 1444 34
Fragments arranged by size:
1444 317 179 34
BbvCI CC'TCA_GC
Cuts at: 0 73 1974
Size: 73 1901
BciVI GTATCCnnnnn_n'
Cuts at: 0 638 1974
Size: 638 1336
BglI GCCn_nnn'nGGC
Cuts at: 0 1405 1563 1974
Size: 1405 158 411
Fragments arranged by size:
1405 411 158
BlpI GC'TnA_GC
Cuts at: 0 1381 1974
Size: 1381 593
Bme1580I G_kGCm'C
Cuts at: 0 8 40 92 122 201 1974
Size: 8 32 52 30 79 1773
Fragments arranged by size:
1773 79 52 32 30 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 56 639 680 1974
Size: 56 583 41 1294
Fragments arranged by size:
1294 583 56 41
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 1545 1974
Size: 532 1013 429
Fragments arranged by size:
1013 532 429
Bpu10I CC'TnA_GC
Cuts at: 0 73 904 1289 1712 1974
Size: 73 831 385 423 262
Fragments arranged by size:
831 423 385 262 73
BsaI GGTCTCn'nnnn_
Cuts at: 0 1492 1821 1974
Size: 1492 329 153
Fragments arranged by size:
1492 329 153
BsaWI w'CCGG_w
Cuts at: 0 479 1974
Size: 479 1495
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 949 1364 1974
Size: 949 415 610
Fragments arranged by size:
949 610 415
BseYI C'CCAG_C
Cuts at: 0 1400 1782 1974
Size: 1400 382 192
Fragments arranged by size:
1400 382 192
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1255 1974
Size: 1255 719
BsiEI CG_ry'CG
Cuts at: 0 1491 1974
Size: 1491 483
BsiHKAI G_wGCw'C
Cuts at: 0 201 905 1115 1142 1537 1606 1718 1771
Size: 201 704 210 27 395 69 112 53
Cuts at: 1771 1974
Size: 203
Fragments arranged by size:
704 395 210 203 201 112 69 53
27
BsmI GAATG_Cn'
Cuts at: 0 1408 1477 1974
Size: 1408 69 497
Fragments arranged by size:
1408 497 69
Bsp1286I G_dGCh'C
Cuts at: 0 8 40 60 92 122 201 796 905
Size: 8 32 20 32 30 79 595 109
Cuts at: 905 1115 1142 1387 1537 1559 1606 1718 1771
Size: 210 27 245 150 22 47 112 53
Cuts at: 1771 1974
Size: 203
Fragments arranged by size:
595 245 210 203 150 112 109 79
53 47 32 32 30 27 22 20
8
BspHI T'CATG_A
Cuts at: 0 1848 1974
Size: 1848 126
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 110 1974
Size: 110 1864
BsrDI GCAATG_nn'
Cuts at: 0 936 1646 1875 1899 1974
Size: 936 710 229 24 75
Fragments arranged by size:
936 710 229 75 24
BsrFI r'CCGG_y
Cuts at: 0 79 1974
Size: 79 1895
BssHII G'CGCG_C
Cuts at: 0 21 1974
Size: 21 1953
BssSI C'ACGA_G
Cuts at: 0 1764 1974
Size: 1764 210
Bsu36I CC'TnA_GG
Cuts at: 0 736 943 1974
Size: 736 207 1031
Fragments arranged by size:
1031 736 207
BtgI C'CryG_G
Cuts at: 0 41 1614 1974
Size: 41 1573 360
Fragments arranged by size:
1573 360 41
BtsI GCAGTG_nn'
Cuts at: 0 968 1142 1469 1793 1974
Size: 968 174 327 324 181
Fragments arranged by size:
968 327 324 181 174
DraI TTT'AAA
Cuts at: 0 1164 1974
Size: 1164 810
DrdI GACnn_nn'nnGTC
Cuts at: 0 1225 1974
Size: 1225 749
EaeI y'GGCC_r
Cuts at: 0 93 222 1448 1488 1617 1974
Size: 93 129 1226 40 129 357
Fragments arranged by size:
1226 357 129 129 93 40
EagI C'GGCC_G
Cuts at: 0 1488 1974
Size: 1488 486
EarI CTCTTCn'nnn_
Cuts at: 0 706 1881 1974
Size: 706 1175 93
Fragments arranged by size:
1175 706 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1495 1974
Size: 1495 479
EcoICRI GAG'CTC
Cuts at: 0 903 1716 1974
Size: 903 813 258
Fragments arranged by size:
903 813 258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 532 838 1387 1545 1567 1974
Size: 508 24 306 549 158 22 407
Fragments arranged by size:
549 508 407 306 158 24 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1548 1974
Size: 1548 426
EcoO109I rG'GnC_Cy
Cuts at: 0 36 37 340 608 999 1974
Size: 36 1 303 268 391 975
Fragments arranged by size:
975 391 303 268 36 1
EcoRI G'AATT_C
Cuts at: 0 1360 1974
Size: 1360 614
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 228 1974
Size: 228 1746
HaeII r_GCGC'y
Cuts at: 0 80 1974
Size: 80 1894
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 409 441 1974
Size: 409 32 1533
Fragments arranged by size:
1533 409 32
MscI TGG'CCA
Cuts at: 0 224 1450 1619 1974
Size: 224 1226 169 355
Fragments arranged by size:
1226 355 224 169
MslI CAynn'nnrTG
Cuts at: 0 1034 1974
Size: 1034 940
MspA1I CmG'CkG
Cuts at: 0 43 294 1049 1285 1404 1623 1974
Size: 43 251 755 236 119 219 351
Fragments arranged by size:
755 351 251 236 219 119 43
NaeI GCC'GGC
Cuts at: 0 81 1974
Size: 81 1893
NcoI C'CATG_G
Cuts at: 0 1614 1974
Size: 1614 360
NgoMIV G'CCGG_C
Cuts at: 0 79 1974
Size: 79 1895
NspI r_CATG'y
Cuts at: 0 1941 1974
Size: 1941 33
PciI A'CATG_T
Cuts at: 0 1937 1974
Size: 1937 37
PflMI CCAn_nnn'nTGG
Cuts at: 0 1917 1974
Size: 1917 57
PfoI T'CCnGG_A
Cuts at: 0 51 767 1974
Size: 51 716 1207
Fragments arranged by size:
1207 716 51
PpuMI rG'GwC_Cy
Cuts at: 0 340 999 1974
Size: 340 659 975
Fragments arranged by size:
975 659 340
PspOMI G'GGCC_C
Cuts at: 0 36 88 1974
Size: 36 52 1886
Fragments arranged by size:
1886 52 36
PstI C_TGCA'G
Cuts at: 0 107 1974
Size: 107 1867
PvuII CAG'CTG
Cuts at: 0 294 1285 1404 1623 1974
Size: 294 991 119 219 351
Fragments arranged by size:
991 351 294 219 119
SacI G_AGCT'C
Cuts at: 0 905 1718 1974
Size: 905 813 256
Fragments arranged by size:
905 813 256
SacII CC_GC'GG
Cuts at: 0 44 1974
Size: 44 1930
SanDI GG'GwC_CC
Cuts at: 0 340 1974
Size: 340 1634
SexAI A'CCwGG_T
Cuts at: 0 614 1974
Size: 614 1360
SfcI C'TryA_G
Cuts at: 0 103 1464 1974
Size: 103 1361 510
Fragments arranged by size:
1361 510 103
SmaI CCC'GGG
Cuts at: 0 34 451 1900 1974
Size: 34 417 1449 74
Fragments arranged by size:
1449 417 74 34
SmlI C'TyrA_G
Cuts at: 0 368 1974
Size: 368 1606
StuI AGG'CCT
Cuts at: 0 1543 1927 1974
Size: 1543 384 47
Fragments arranged by size:
1543 384 47
StyI C'CwwG_G
Cuts at: 0 1082 1614 1794 1974
Size: 1082 532 180 180
Fragments arranged by size:
1082 532 180 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1673 1974
Size: 1673 301
TatI w'GTAC_w
Cuts at: 0 821 1974
Size: 821 1153
Tth111I GACn'n_nGTC
Cuts at: 0 1003 1333 1974
Size: 1003 330 641
Fragments arranged by size:
1003 641 330
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 726 1974
Size: 726 1248
XhoI C'TCGA_G
Cuts at: 0 368 1974
Size: 368 1606
XmaI C'CCGG_G
Cuts at: 0 32 449 1898 1974
Size: 32 417 1449 76
Fragments arranged by size:
1449 417 76 32
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaI ApaLI ApoI AvaI
BaeI BanI BanII BbsI BbvCI BciVI BglI BlpI
Bme1580I BmrI BpmI Bpu10I BsaI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BsrFI BssHII BssSI Bsu36I BtgI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PciI PflMI PfoI PpuMI PspOMI
PstI PvuII SacI SacII SanDI SexAI SfcI SmaI
SmlI StuI StyI TaqII TatI Tth111I XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI AscI AseI AsiSI AvrII BamHI
BbeI BcgI BclI BfrBI BglII BmgBI BmtI BplI
BpuEI BsaAI BsaBI BsaHI BsaXI BsiWI BsmBI BspEI
BsrBI BsrGI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgZI ClaI CspCI DraIII EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NarI NdeI NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI
XbaI XmnI ZraI