Restriction map for
pF1KE2428
(Linear) (Six-base) MAPSORT of: KIEE2428.seq Check: 8246 from: 1 to: 1047
KIEE2428.orf
With 182 enzymes: SgfI *
November 16, 2012 10:47 ..
AccI GT'mk_AC
Cuts at: 0 365 947 1047
Size: 365 582 100
Fragments arranged by size:
582 365 100
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 406 1047
Size: 346 60 641
Fragments arranged by size:
641 346 60
AleI CACnn'nnGTG
Cuts at: 0 568 1047
Size: 568 479
AlwNI CAG_nnn'CTG
Cuts at: 0 888 1047
Size: 888 159
ApaI G_GGCC'C
Cuts at: 0 376 899 1047
Size: 376 523 148
Fragments arranged by size:
523 376 148
ApoI r'AATT_y
Cuts at: 0 1009 1047
Size: 1009 38
AvaI C'yCGr_G
Cuts at: 0 898 908 1047
Size: 898 10 139
Fragments arranged by size:
898 139 10
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 543 576 1047
Size: 543 33 471
Fragments arranged by size:
543 471 33
BanI G'GyrC_C
Cuts at: 0 276 1047
Size: 276 771
BanII G_rGCy'C
Cuts at: 0 46 376 899 1047
Size: 46 330 523 148
Fragments arranged by size:
523 330 148 46
BbsI GAAGACnn'nnnn_
Cuts at: 0 134 1047
Size: 134 913
BbvCI CC'TCA_GC
Cuts at: 0 821 1047
Size: 821 226
BclI T'GATC_A
Cuts at: 0 782 1047
Size: 782 265
BglI GCCn_nnn'nGGC
Cuts at: 0 85 628 1047
Size: 85 543 419
Fragments arranged by size:
543 419 85
BglII A'GATC_T
Cuts at: 0 299 593 1047
Size: 299 294 454
Fragments arranged by size:
454 299 294
BlpI GC'TnA_GC
Cuts at: 0 45 1047
Size: 45 1002
Bme1580I G_kGCm'C
Cuts at: 0 200 281 376 899 1047
Size: 200 81 95 523 148
Fragments arranged by size:
523 200 148 95 81
BmrI ACTGGGnnnn_n'
Cuts at: 0 48 1047
Size: 48 999
BmtI G_CTAG'C
Cuts at: 0 8 1047
Size: 8 1039
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 913 1047
Size: 880 33 134
Fragments arranged by size:
880 134 33
Bpu10I CC'TnA_GC
Cuts at: 0 821 1047
Size: 821 226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 1047
Size: 298 749
BsaWI w'CCGG_w
Cuts at: 0 809 1047
Size: 809 238
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 853 883 1047
Size: 853 30 164
Fragments arranged by size:
853 164 30
BseYI C'CCAG_C
Cuts at: 0 80 687 1047
Size: 80 607 360
Fragments arranged by size:
607 360 80
Bsp1286I G_dGCh'C
Cuts at: 0 46 200 281 376 899 1047
Size: 46 154 81 95 523 148
Fragments arranged by size:
523 154 148 95 81 46
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 197 799 1047
Size: 197 602 248
Fragments arranged by size:
602 248 197
BsrDI GCAATG_nn'
Cuts at: 0 78 452 1047
Size: 78 374 595
Fragments arranged by size:
595 374 78
BsrGI T'GTAC_A
Cuts at: 0 974 1047
Size: 974 73
BstAPI GCAn_nnn'nTGC
Cuts at: 0 204 253 888 1047
Size: 204 49 635 159
Fragments arranged by size:
635 204 159 49
BstEII G'GTnAC_C
Cuts at: 0 105 1047
Size: 105 942
BstXI CCAn_nnnn'nTGG
Cuts at: 0 63 465 756 1047
Size: 63 402 291 291
Fragments arranged by size:
402 291 291 63
BstYI r'GATC_y
Cuts at: 0 299 593 1047
Size: 299 294 454
Fragments arranged by size:
454 299 294
BtgI C'CryG_G
Cuts at: 0 100 623 1047
Size: 100 523 424
Fragments arranged by size:
523 424 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 36 1047
Size: 36 1011
BtsI GCAGTG_nn'
Cuts at: 0 683 735 1017 1047
Size: 683 52 282 30
Fragments arranged by size:
683 282 52 30
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 200 235 1047
Size: 200 35 812
Fragments arranged by size:
812 200 35
EaeI y'GGCC_r
Cuts at: 0 620 1047
Size: 620 427
EarI CTCTTCn'nnn_
Cuts at: 0 727 1047
Size: 727 320
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 819 1047
Size: 819 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 406 880 913 1047
Size: 346 60 474 33 134
Fragments arranged by size:
474 346 134 60 33
EcoO109I rG'GnC_Cy
Cuts at: 0 372 373 507 895 939 1047
Size: 372 1 134 388 44 108
Fragments arranged by size:
388 372 134 108 44 1
EcoRI G'AATT_C
Cuts at: 0 1009 1047
Size: 1009 38
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 975 1047
Size: 975 72
HaeII r_GCGC'y
Cuts at: 0 417 1047
Size: 417 630
MslI CAynn'nnrTG
Cuts at: 0 176 427 433 568 700 970 1047
Size: 176 251 6 135 132 270 77
Fragments arranged by size:
270 251 176 135 132 77 6
MspA1I CmG'CkG
Cuts at: 0 84 858 1047
Size: 84 774 189
Fragments arranged by size:
774 189 84
NdeI CA'TA_TG
Cuts at: 0 1043 1047
Size: 1043 4
NheI G'CTAG_C
Cuts at: 0 4 1047
Size: 4 1043
PasI CC'CwG_GG
Cuts at: 0 516 1047
Size: 516 531
PflMI CCAn_nnn'nTGG
Cuts at: 0 1002 1047
Size: 1002 45
PfoI T'CCnGG_A
Cuts at: 0 31 1047
Size: 31 1016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 1047
Size: 28 32 987
Fragments arranged by size:
987 32 28
PpuMI rG'GwC_Cy
Cuts at: 0 507 1047
Size: 507 540
PspOMI G'GGCC_C
Cuts at: 0 372 895 1047
Size: 372 523 152
Fragments arranged by size:
523 372 152
PstI C_TGCA'G
Cuts at: 0 249 730 893 1028 1047
Size: 249 481 163 135 19
Fragments arranged by size:
481 249 163 135 19
PvuII CAG'CTG
Cuts at: 0 84 858 1047
Size: 84 774 189
Fragments arranged by size:
774 189 84
SapI GCTCTTCn'nnn_
Cuts at: 0 727 1047
Size: 727 320
SfcI C'TryA_G
Cuts at: 0 245 726 889 1024 1047
Size: 245 481 163 135 23
Fragments arranged by size:
481 245 163 135 23
SmaI CCC'GGG
Cuts at: 0 910 1047
Size: 910 137
SmlI C'TyrA_G
Cuts at: 0 313 1047
Size: 313 734
StuI AGG'CCT
Cuts at: 0 826 1047
Size: 826 221
StyI C'CwwG_G
Cuts at: 0 668 1047
Size: 668 379
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 55 994 1047
Size: 55 939 53
Fragments arranged by size:
939 55 53
TatI w'GTAC_w
Cuts at: 0 974 1047
Size: 974 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 539 571 1047
Size: 539 32 476
Fragments arranged by size:
539 476 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 855 1047
Size: 855 192
XmaI C'CCGG_G
Cuts at: 0 908 1047
Size: 908 139
Enzymes that do cut:
AccI AcuI AleI AlwNI ApaI ApoI AvaI BaeI
BanI BanII BbsI BbvCI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaWI BsaXI
BseYI Bsp1286I BspMI BsrDI BsrGI BstAPI BstEII BstXI
BstYI BtgI BtgZI BtsI CspCI EaeI EarI EciI
Eco57MI EcoO109I EcoRI FalI HaeII MslI MspA1I NdeI
NheI PasI PflMI PfoI PpiI PpuMI PspOMI PstI
PvuII SapI SfcI SmaI SmlI StuI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BamHI BbeI BcgI BciVI BfrBI BmgBI BplI BsaI
BsaAI BsaBI BsaHI BseRI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI
BstBI BstZ17I Bsu36I ClaI DraI DraIII DrdI EagI
EcoICRI EcoNI EcoRV FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NcoI NgoMIV NotI NruI NsiI NspI
PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI