Restriction map for
pF1KE2395
(Linear) (Six-base) MAPSORT of: KIEE2395.seq Check: 9404 from: 1 to: 3387
KIEE2395.orf
With 182 enzymes: SgfI *
January 26, 2012 13:40 ..
AccI GT'mk_AC
Cuts at: 0 868 3387
Size: 868 2519
Acc65I G'GTAC_C
Cuts at: 0 1906 3387
Size: 1906 1481
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 1082 1922 3197 3254 3387
Size: 266 816 840 1275 57 133
Fragments arranged by size:
1275 840 816 266 133 57
AflIII A'CryG_T
Cuts at: 0 505 3252 3387
Size: 505 2747 135
Fragments arranged by size:
2747 505 135
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1406 2936 3387
Size: 1406 1530 451
Fragments arranged by size:
1530 1406 451
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2644 2676 3387
Size: 2644 32 711
Fragments arranged by size:
2644 711 32
ApoI r'AATT_y
Cuts at: 0 491 1835 2332 2379 2673 2781 2946 3312
Size: 491 1344 497 47 294 108 165 366
Cuts at: 3312 3387
Size: 75
Fragments arranged by size:
1344 497 491 366 294 165 108 75
47
AseI AT'TA_AT
Cuts at: 0 152 749 2416 3387
Size: 152 597 1667 971
Fragments arranged by size:
1667 971 597 152
BanI G'GyrC_C
Cuts at: 0 1906 3387
Size: 1906 1481
BbsI GAAGACnn'nnnn_
Cuts at: 0 128 1088 1174 3387
Size: 128 960 86 2213
Fragments arranged by size:
2213 960 128 86
BbvCI CC'TCA_GC
Cuts at: 0 2594 3387
Size: 2594 793
BciVI GTATCCnnnnn_n'
Cuts at: 0 2047 3387
Size: 2047 1340
BclI T'GATC_A
Cuts at: 0 2016 3387
Size: 2016 1371
BglII A'GATC_T
Cuts at: 0 1470 3387
Size: 1470 1917
Bme1580I G_kGCm'C
Cuts at: 0 1653 3387
Size: 1653 1734
BmrI ACTGGGnnnn_n'
Cuts at: 0 911 2034 3387
Size: 911 1123 1353
Fragments arranged by size:
1353 1123 911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2344 3387
Size: 2344 1043
Bpu10I CC'TnA_GC
Cuts at: 0 2594 2909 3387
Size: 2594 315 478
Fragments arranged by size:
2594 478 315
BsaI GGTCTCn'nnnn_
Cuts at: 0 719 3387
Size: 719 2668
BsaAI yAC'GTr
Cuts at: 0 1920 1970 3387
Size: 1920 50 1417
Fragments arranged by size:
1920 1417 50
BsaBI GATnn'nnATC
Cuts at: 0 137 1784 3387
Size: 137 1647 1603
Fragments arranged by size:
1647 1603 137
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 699 729 1959 1989 3387
Size: 699 30 1230 30 1398
Fragments arranged by size:
1398 1230 699 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1207 1210 2191 3017 3387
Size: 1207 3 981 826 370
Fragments arranged by size:
1207 981 826 370 3
BseYI C'CCAG_C
Cuts at: 0 1815 2051 3012 3387
Size: 1815 236 961 375
Fragments arranged by size:
1815 961 375 236
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1525 2723 2825 3387
Size: 1525 1198 102 562
Fragments arranged by size:
1525 1198 562 102
BsiHKAI G_wGCw'C
Cuts at: 0 2000 2257 3387
Size: 2000 257 1130
Fragments arranged by size:
2000 1130 257
BsmI GAATG_Cn'
Cuts at: 0 1220 1949 2066 2529 3387
Size: 1220 729 117 463 858
Fragments arranged by size:
1220 858 729 463 117
Bsp1286I G_dGCh'C
Cuts at: 0 1653 2000 2257 3387
Size: 1653 347 257 1130
Fragments arranged by size:
1653 1130 347 257
BspHI T'CATG_A
Cuts at: 0 1088 3387
Size: 1088 2299
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 730 3387
Size: 730 2657
BstBI TT'CG_AA
Cuts at: 0 1941 3387
Size: 1941 1446
BstEII G'GTnAC_C
Cuts at: 0 1846 3387
Size: 1846 1541
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1401 3387
Size: 1401 1986
BstYI r'GATC_y
Cuts at: 0 334 472 1034 1470 3387
Size: 334 138 562 436 1917
Fragments arranged by size:
1917 562 436 334 138
BstZ17I GTA'TAC
Cuts at: 0 869 3387
Size: 869 2518
Bsu36I CC'TnA_GG
Cuts at: 0 437 3387
Size: 437 2950
BtsI GCAGTG_nn'
Cuts at: 0 203 523 1533 1998 2837 3387
Size: 203 320 1010 465 839 550
Fragments arranged by size:
1010 839 550 465 320 203
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 278 313 596 631 2462 2497 3387
Size: 278 35 283 35 1831 35 890
Fragments arranged by size:
1831 890 283 278 35 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1118 3387
Size: 1118 2269
EaeI y'GGCC_r
Cuts at: 0 520 3387
Size: 520 2867
EarI CTCTTCn'nnn_
Cuts at: 0 181 636 1859 2243 2480 3387
Size: 181 455 1223 384 237 907
Fragments arranged by size:
1223 907 455 384 237 181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 1082 1922 2344 3197 3254 3387
Size: 266 816 840 422 853 57 133
Fragments arranged by size:
853 840 816 422 266 133 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2075 3387
Size: 2075 1312
EcoRI G'AATT_C
Cuts at: 0 2332 2781 3387
Size: 2332 449 606
Fragments arranged by size:
2332 606 449
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 462 665 3004 3387
Size: 462 203 2339 383
Fragments arranged by size:
2339 462 383 203
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1129 1161 3387
Size: 1129 32 2226
Fragments arranged by size:
2226 1129 32
HincII GTy'rAC
Cuts at: 0 3173 3387
Size: 3173 214
HindIII A'AGCT_T
Cuts at: 0 1528 2134 3387
Size: 1528 606 1253
Fragments arranged by size:
1528 1253 606
KpnI G_GTAC'C
Cuts at: 0 1910 3387
Size: 1910 1477
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 3387
Size: 138 3249
MscI TGG'CCA
Cuts at: 0 522 3387
Size: 522 2865
MslI CAynn'nnrTG
Cuts at: 0 174 1399 2776 3387
Size: 174 1225 1377 611
Fragments arranged by size:
1377 1225 611 174
MspA1I CmG'CkG
Cuts at: 0 1900 3387
Size: 1900 1487
NdeI CA'TA_TG
Cuts at: 0 1612 3387
Size: 1612 1775
NspI r_CATG'y
Cuts at: 0 509 1502 3387
Size: 509 993 1885
Fragments arranged by size:
1885 993 509
PciI A'CATG_T
Cuts at: 0 505 3387
Size: 505 2882
PflMI CCAn_nnn'nTGG
Cuts at: 0 592 3387
Size: 592 2795
PsiI TTA'TAA
Cuts at: 0 968 1060 2881 3387
Size: 968 92 1821 506
Fragments arranged by size:
1821 968 506 92
PstI C_TGCA'G
Cuts at: 0 210 571 741 2925 3387
Size: 210 361 170 2184 462
Fragments arranged by size:
2184 462 361 210 170
PvuII CAG'CTG
Cuts at: 0 1900 3387
Size: 1900 1487
SexAI A'CCwGG_T
Cuts at: 0 709 3387
Size: 709 2678
SfcI C'TryA_G
Cuts at: 0 206 567 737 2447 2546 2921 3387
Size: 206 361 170 1710 99 375 466
Fragments arranged by size:
1710 466 375 361 206 170 99
SpeI A'CTAG_T
Cuts at: 0 1645 3387
Size: 1645 1742
SspI AAT'ATT
Cuts at: 0 730 2802 3096 3387
Size: 730 2072 294 291
Fragments arranged by size:
2072 730 294 291
StyI C'CwwG_G
Cuts at: 0 1794 2563 2721 3387
Size: 1794 769 158 666
Fragments arranged by size:
1794 769 666 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1609 2016 3387
Size: 1609 407 1371
Fragments arranged by size:
1609 1371 407
TatI w'GTAC_w
Cuts at: 0 2668 3387
Size: 2668 719
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1703 1735 3387
Size: 1703 32 1652
Fragments arranged by size:
1703 1652 32
XbaI T'CTAG_A
Cuts at: 0 897 3387
Size: 897 2490
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 789 3387
Size: 789 2598
XmnI GAAnn'nnTTC
Cuts at: 0 251 3387
Size: 251 3136
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlfI AloI ApoI AseI
BanI BbsI BbvCI BciVI BclI BglII Bme1580I BmrI
BpmI Bpu10I BsaI BsaAI BsaBI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtsI CspCI DrdI EaeI
EarI Eco57MI EcoNI EcoRI FalI Hin4I HincII HindIII
KpnI MmeI MscI MslI MspA1I NdeI NspI PciI
PflMI PsiI PstI PvuII SexAI SfcI SpeI SspI
StyI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BanII BbeI BcgI BfrBI BglI BlpI BmgBI
BmtI BplI BpuEI BsaHI BsaWI BsiEI BsiWI BsmBI
BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BtgI BtgZI ClaI DraI DraIII EagI EciI EcoICRI
EcoO109I EcoRV FseI FspI FspAI HaeII HpaI KasI
MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PasI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SphI SrfI StuI
SwaI Tth111I XhoI XmaI ZraI