Restriction map for
pF1KE2381
(Linear) (Six-base) MAPSORT of: KIEE2381.seq Check: 1837 from: 1 to: 4470
KIEE2381.orf
With 182 enzymes: SgfI *
October 25, 2011 13:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3953 4470
Size: 3953 517
AatII G_ACGT'C
Cuts at: 0 3147 4470
Size: 3147 1323
AccI GT'mk_AC
Cuts at: 0 2943 4470
Size: 2943 1527
Acc65I G'GTAC_C
Cuts at: 0 2699 4470
Size: 2699 1771
AclI AA'CG_TT
Cuts at: 0 1876 2733 4470
Size: 1876 857 1737
Fragments arranged by size:
1876 1737 857
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 1409 2284 2678 3554 3944 4282 4470
Size: 589 820 875 394 876 390 338 188
Fragments arranged by size:
876 875 820 589 394 390 338 188
AflIII A'CryG_T
Cuts at: 0 1984 4470
Size: 1984 2486
AleI CACnn'nnGTG
Cuts at: 0 2351 4470
Size: 2351 2119
AlwNI CAG_nnn'CTG
Cuts at: 0 1225 1378 3447 4107 4470
Size: 1225 153 2069 660 363
Fragments arranged by size:
2069 1225 660 363 153
ApaI G_GGCC'C
Cuts at: 0 3722 4384 4470
Size: 3722 662 86
Fragments arranged by size:
3722 662 86
ApaLI G'TGCA_C
Cuts at: 0 2485 3024 4470
Size: 2485 539 1446
Fragments arranged by size:
2485 1446 539
ApoI r'AATT_y
Cuts at: 0 1419 1797 2108 2559 2953 4470
Size: 1419 378 311 451 394 1517
Fragments arranged by size:
1517 1419 451 394 378 311
AvaI C'yCGr_G
Cuts at: 0 356 711 765 807 4470
Size: 356 355 54 42 3663
Fragments arranged by size:
3663 356 355 54 42
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1820 1853 4470
Size: 1820 33 2617
Fragments arranged by size:
2617 1820 33
BanI G'GyrC_C
Cuts at: 0 2419 2699 4470
Size: 2419 280 1771
Fragments arranged by size:
2419 1771 280
BanII G_rGCy'C
Cuts at: 0 667 880 2024 3722 3806 4384 4470
Size: 667 213 1144 1698 84 578 86
Fragments arranged by size:
1698 1144 667 578 213 86 84
BbsI GAAGACnn'nnnn_
Cuts at: 0 553 1834 2142 4385 4470
Size: 553 1281 308 2243 85
Fragments arranged by size:
2243 1281 553 308 85
BbvCI CC'TCA_GC
Cuts at: 0 2081 3260 4268 4470
Size: 2081 1179 1008 202
Fragments arranged by size:
2081 1179 1008 202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 313 347 4470
Size: 313 34 4123
Fragments arranged by size:
4123 313 34
BclI T'GATC_A
Cuts at: 0 2840 4470
Size: 2840 1630
BglI GCCn_nnn'nGGC
Cuts at: 0 3717 4470
Size: 3717 753
BglII A'GATC_T
Cuts at: 0 898 2034 4470
Size: 898 1136 2436
Fragments arranged by size:
2436 1136 898
BlpI GC'TnA_GC
Cuts at: 0 2828 4470
Size: 2828 1642
Bme1580I G_kGCm'C
Cuts at: 0 193 581 2489 3028 3477 3722 4174 4384
Size: 193 388 1908 539 449 245 452 210
Cuts at: 4384 4470
Size: 86
Fragments arranged by size:
1908 539 452 449 388 245 210 193
86
BmgBI CAC'GTC
Cuts at: 0 1987 4470
Size: 1987 2483
BmrI ACTGGGnnnn_n'
Cuts at: 0 965 1037 2163 3550 4352 4383 4470
Size: 965 72 1126 1387 802 31 87
Fragments arranged by size:
1387 1126 965 802 87 72 31
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1210 3410 4470
Size: 1210 2200 1060
Fragments arranged by size:
2200 1210 1060
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 1931 2000 2039 2693 3074 3700 4470
Size: 393 1538 69 39 654 381 626 770
Fragments arranged by size:
1538 770 654 626 393 381 69 39
Bpu10I CC'TnA_GC
Cuts at: 0 1009 1310 2081 3260 3385 3800 4227 4268
Size: 1009 301 771 1179 125 415 427 41
Cuts at: 4268 4356 4470
Size: 88 114
Fragments arranged by size:
1179 1009 771 427 415 301 125 114
88 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1817 3660 3987 4470
Size: 1817 1843 327 483
Fragments arranged by size:
1843 1817 483 327
BsaI GGTCTCn'nnnn_
Cuts at: 0 1025 1564 2094 3505 3963 4294 4470
Size: 1025 539 530 1411 458 331 176
Fragments arranged by size:
1411 1025 539 530 458 331 176
BsaHI Gr'CG_yC
Cuts at: 0 2940 3144 4470
Size: 2940 204 1326
Fragments arranged by size:
2940 1326 204
BsaWI w'CCGG_w
Cuts at: 0 434 587 4470
Size: 434 153 3883
Fragments arranged by size:
3883 434 153
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 232 262 556 582 586 612 1614 1644
Size: 232 30 294 26 4 26 1002 30
Cuts at: 1644 1866 1896 2240 2270 3406 3436 4470
Size: 222 30 344 30 1136 30 1034
Fragments arranged by size:
1136 1034 1002 344 294 232 222 30
30 30 30 30 26 26 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 59 481 1543 3077 3541 3758 3763 4470
Size: 59 422 1062 1534 464 217 5 707
Fragments arranged by size:
1534 1062 707 464 422 217 59 5
BseYI C'CCAG_C
Cuts at: 0 3249 3292 3494 3882 3917 4326 4470
Size: 3249 43 202 388 35 409 144
Fragments arranged by size:
3249 409 388 202 144 43 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 4204 4470
Size: 307 3897 266
Fragments arranged by size:
3897 307 266
BsiEI CG_ry'CG
Cuts at: 0 318 928 4470
Size: 318 610 3542
Fragments arranged by size:
3542 610 318
BsiHKAI G_wGCw'C
Cuts at: 0 2024 2489 3028 3959 4470
Size: 2024 465 539 931 511
Fragments arranged by size:
2024 931 539 511 465
BsmBI CGTCTCn'nnnn_
Cuts at: 0 915 4470
Size: 915 3555
Bsp1286I G_dGCh'C
Cuts at: 0 193 581 667 880 2024 2489 3028 3477
Size: 193 388 86 213 1144 465 539 449
Cuts at: 3477 3722 3806 3959 4174 4384 4470
Size: 245 84 153 215 210 86
Fragments arranged by size:
1144 539 465 449 388 245 215 213
210 193 153 86 86 84
BspHI T'CATG_A
Cuts at: 0 2516 4470
Size: 2516 1954
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 483 3371 3953 4470
Size: 483 2888 582 517
Fragments arranged by size:
2888 582 517 483
BsrGI T'GTAC_A
Cuts at: 0 1812 4470
Size: 1812 2658
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1225 3550 4470
Size: 1225 2325 920
Fragments arranged by size:
2325 1225 920
BstBI TT'CG_AA
Cuts at: 0 460 4470
Size: 460 4010
BstEII G'GTnAC_C
Cuts at: 0 165 869 4470
Size: 165 704 3601
Fragments arranged by size:
3601 704 165
BstXI CCAn_nnnn'nTGG
Cuts at: 0 156 2876 4470
Size: 156 2720 1594
Fragments arranged by size:
2720 1594 156
BstYI r'GATC_y
Cuts at: 0 655 898 1120 2034 3009 4470
Size: 655 243 222 914 975 1461
Fragments arranged by size:
1461 975 914 655 243 222
Bsu36I CC'TnA_GG
Cuts at: 0 3213 4470
Size: 3213 1257
BtgI C'CryG_G
Cuts at: 0 3515 4470
Size: 3515 955
BtsI GCAGTG_nn'
Cuts at: 0 3356 4470
Size: 3356 1114
ClaI AT'CG_AT
Cuts at: 0 424 4470
Size: 424 4046
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3687 3722 4470
Size: 3687 35 748
Fragments arranged by size:
3687 748 35
DraIII CAC_nnn'GTG
Cuts at: 0 4242 4470
Size: 4242 228
EaeI y'GGCC_r
Cuts at: 0 726 2214 3939 4470
Size: 726 1488 1725 531
Fragments arranged by size:
1725 1488 726 531
EarI CTCTTCn'nnn_
Cuts at: 0 1111 1589 1682 1949 2654 3167 3784 4443
Size: 1111 478 93 267 705 513 617 659
Cuts at: 4443 4470
Size: 27
Fragments arranged by size:
1111 705 659 617 513 478 267 93
27
EcoICRI GAG'CTC
Cuts at: 0 2022 4470
Size: 2022 2448
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 589 1409 1931 2000 2039 2284 2678
Size: 393 196 820 522 69 39 245 394
Cuts at: 2678 2693 3074 3554 3700 3944 4282 4470
Size: 15 381 480 146 244 338 188
Fragments arranged by size:
820 522 480 394 393 381 338 245
244 196 188 146 69 39 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 810 885 915 4034 4470
Size: 810 75 30 3119 436
Fragments arranged by size:
3119 810 436 75 30
EcoO109I rG'GnC_Cy
Cuts at: 0 3718 3719 3794 4121 4203 4221 4380 4420
Size: 3718 1 75 327 82 18 159 40
Cuts at: 4420 4470
Size: 50
Fragments arranged by size:
3718 327 159 82 75 50 40 18
1
EcoRI G'AATT_C
Cuts at: 0 2953 4470
Size: 2953 1517
EcoRV GAT'ATC
Cuts at: 0 225 440 4470
Size: 225 215 4030
Fragments arranged by size:
4030 225 215
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 968 1960 3871 4470
Size: 968 992 1911 599
Fragments arranged by size:
1911 992 968 599
HaeII r_GCGC'y
Cuts at: 0 3866 4470
Size: 3866 604
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 214 232 246 264 424 456 556 582
Size: 214 18 14 18 160 32 100 26
Cuts at: 582 588 614 694 726 1017 1049 1441 1473
Size: 6 26 80 32 291 32 392 32
Cuts at: 1473 1524 1556 1971 2003 2994 3026 4470
Size: 51 32 415 32 991 32 1444
Fragments arranged by size:
1444 991 415 392 291 214 160 100
80 51 32 32 32 32 32 32
32 26 26 18 18 14 6
HincII GTy'rAC
Cuts at: 0 2361 4470
Size: 2361 2109
HindIII A'AGCT_T
Cuts at: 0 3593 4470
Size: 3593 877
HpaI GTT'AAC
Cuts at: 0 2361 4470
Size: 2361 2109
KpnI G_GTAC'C
Cuts at: 0 2703 4470
Size: 2703 1767
MfeI C'AATT_G
Cuts at: 0 1592 4470
Size: 1592 2878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 1009 1427 3449 4470
Size: 263 746 418 2022 1021
Fragments arranged by size:
2022 1021 746 418 263
MscI TGG'CCA
Cuts at: 0 728 2216 3941 4470
Size: 728 1488 1725 529
Fragments arranged by size:
1725 1488 728 529
MslI CAynn'nnrTG
Cuts at: 0 2351 4470
Size: 2351 2119
MspA1I CmG'CkG
Cuts at: 0 959 1222 1849 2872 3031 3330 3408 3447
Size: 959 263 627 1023 159 299 78 39
Cuts at: 3447 3610 3648 4470
Size: 163 38 822
Fragments arranged by size:
1023 959 822 627 299 263 163 159
78 39 38
NcoI C'CATG_G
Cuts at: 0 3515 4470
Size: 3515 955
NspI r_CATG'y
Cuts at: 0 3000 3618 3764 4297 4470
Size: 3000 618 146 533 173
Fragments arranged by size:
3000 618 533 173 146
PasI CC'CwG_GG
Cuts at: 0 186 4470
Size: 186 4284
PflMI CCAn_nnn'nTGG
Cuts at: 0 4107 4470
Size: 4107 363
PfoI T'CCnGG_A
Cuts at: 0 658 795 4470
Size: 658 137 3675
Fragments arranged by size:
3675 658 137
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1110 1142 4470
Size: 1110 32 3328
Fragments arranged by size:
3328 1110 32
PpuMI rG'GwC_Cy
Cuts at: 0 4121 4203 4221 4470
Size: 4121 82 18 249
Fragments arranged by size:
4121 249 82 18
PshAI GACnn'nnGTC
Cuts at: 0 293 743 927 4470
Size: 293 450 184 3543
Fragments arranged by size:
3543 450 293 184
PspOMI G'GGCC_C
Cuts at: 0 3718 4380 4470
Size: 3718 662 90
Fragments arranged by size:
3718 662 90
PspXI vC'TCGA_Gb
Cuts at: 0 765 4470
Size: 765 3705
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3026 3058 4470
Size: 3026 32 1412
Fragments arranged by size:
3026 1412 32
PstI C_TGCA'G
Cuts at: 0 1236 2976 3371 4470
Size: 1236 1740 395 1099
Fragments arranged by size:
1740 1236 1099 395
PvuII CAG'CTG
Cuts at: 0 1222 1849 2872 3031 3330 3408 3447 3610
Size: 1222 627 1023 159 299 78 39 163
Cuts at: 3610 3648 4470
Size: 38 822
Fragments arranged by size:
1222 1023 822 627 299 163 159 78
39 38
SacI G_AGCT'C
Cuts at: 0 2024 4470
Size: 2024 2446
SanDI GG'GwC_CC
Cuts at: 0 4221 4470
Size: 4221 249
SapI GCTCTTCn'nnn_
Cuts at: 0 1682 3167 4470
Size: 1682 1485 1303
Fragments arranged by size:
1682 1485 1303
ScaI AGT'ACT
Cuts at: 0 1740 3225 3982 4470
Size: 1740 1485 757 488
Fragments arranged by size:
1740 1485 757 488
SfcI C'TryA_G
Cuts at: 0 882 912 1232 2972 3043 3367 4470
Size: 882 30 320 1740 71 324 1103
Fragments arranged by size:
1740 1103 882 324 320 71 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3717 4470
Size: 3717 753
SmaI CCC'GGG
Cuts at: 0 358 4470
Size: 358 4112
SmlI C'TyrA_G
Cuts at: 0 765 807 1832 3639 3966 4470
Size: 765 42 1025 1807 327 504
Fragments arranged by size:
1807 1025 765 504 327 42
SpeI A'CTAG_T
Cuts at: 0 2253 4470
Size: 2253 2217
StuI AGG'CCT
Cuts at: 0 3308 3964 4367 4470
Size: 3308 656 403 103
Fragments arranged by size:
3308 656 403 103
StyI C'CwwG_G
Cuts at: 0 1259 2231 3515 4094 4146 4470
Size: 1259 972 1284 579 52 324
Fragments arranged by size:
1284 1259 972 579 324 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 290 397 633 810 984 4470
Size: 290 107 236 177 174 3486
Fragments arranged by size:
3486 290 236 177 174 107
TatI w'GTAC_w
Cuts at: 0 1738 1812 3223 3980 4470
Size: 1738 74 1411 757 490
Fragments arranged by size:
1738 1411 757 490 74
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 337 369 1989 2021 2151 2183 2240 2272
Size: 337 32 1620 32 130 32 57 32
Cuts at: 2272 2566 2598 4470
Size: 294 32 1872
Fragments arranged by size:
1872 1620 337 294 130 57 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3995 4470
Size: 3995 475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2820 4470
Size: 2820 1650
XhoI C'TCGA_G
Cuts at: 0 765 807 4470
Size: 765 42 3663
Fragments arranged by size:
3663 765 42
XmaI C'CCGG_G
Cuts at: 0 356 4470
Size: 356 4114
XmnI GAAnn'nnTTC
Cuts at: 0 1943 4470
Size: 1943 2527
ZraI GAC'GTC
Cuts at: 0 3145 4470
Size: 3145 1325
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlwNI ApaI ApaLI ApoI AvaI BaeI BanI BanII
BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrGI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtsI ClaI CspCI DraIII EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MmeI
MscI MslI MspA1I NcoI NspI PasI PflMI PfoI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuII
SacI SanDI SapI ScaI SfcI SfiI SmaI SmlI
SpeI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AlfI AloI AscI AseI
AsiSI AvrII BamHI BbeI BciVI BfrBI BmtI BsaAI
BsaBI BsiWI BsmI BspEI BsrBI BsrDI BsrFI BssHII
BssSI BstZ17I BtgZI DraI DrdI EagI EciI FseI
FspI FspAI KasI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PsiI PvuI RsrII SacII SalI SbfI SexAI SfoI
SgfI SgrAI SnaBI SphI SrfI SspI SwaI XbaI