Restriction map for
pF1KE2365
(Linear) (Six-base) MAPSORT of: KIEE2365.seq Check: 508 from: 1 to: 729
KIEE2365.orf
With 182 enzymes: SgfI *
August 4, 2011 11:52 ..
AleI CACnn'nnGTG
Cuts at: 0 313 729
Size: 313 416
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 92 729
Size: 92 637
AlwNI CAG_nnn'CTG
Cuts at: 0 702 729
Size: 702 27
ApaI G_GGCC'C
Cuts at: 0 496 729
Size: 496 233
AvaI C'yCGr_G
Cuts at: 0 364 729
Size: 364 365
BamHI G'GATC_C
Cuts at: 0 716 729
Size: 716 13
BanI G'GyrC_C
Cuts at: 0 177 729
Size: 177 552
BanII G_rGCy'C
Cuts at: 0 364 496 600 729
Size: 364 132 104 129
Fragments arranged by size:
364 132 129 104
BbsI GAAGACnn'nnnn_
Cuts at: 0 332 729
Size: 332 397
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 336 370 729
Size: 336 34 359
Fragments arranged by size:
359 336 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 664 729
Size: 664 65
Bme1580I G_kGCm'C
Cuts at: 0 496 729
Size: 496 233
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 729
Size: 49 680
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 608 729
Size: 433 175 121
Fragments arranged by size:
433 175 121
BsaI GGTCTCn'nnnn_
Cuts at: 0 475 729
Size: 475 254
BsaHI Gr'CG_yC
Cuts at: 0 536 729
Size: 536 193
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 10 606 729
Size: 10 596 123
Fragments arranged by size:
596 123 10
BseYI C'CCAG_C
Cuts at: 0 425 729
Size: 425 304
BsiEI CG_ry'CG
Cuts at: 0 7 729
Size: 7 722
Bsp1286I G_dGCh'C
Cuts at: 0 364 496 600 729
Size: 364 132 104 129
Fragments arranged by size:
364 132 129 104
BspHI T'CATG_A
Cuts at: 0 611 729
Size: 611 118
BsrFI r'CCGG_y
Cuts at: 0 290 464 584 729
Size: 290 174 120 145
Fragments arranged by size:
290 174 145 120
BssSI C'ACGA_G
Cuts at: 0 199 729
Size: 199 530
BstEII G'GTnAC_C
Cuts at: 0 467 729
Size: 467 262
BstXI CCAn_nnnn'nTGG
Cuts at: 0 105 729
Size: 105 624
BstYI r'GATC_y
Cuts at: 0 716 729
Size: 716 13
BtgI C'CryG_G
Cuts at: 0 7 48 240 712 729
Size: 7 41 192 472 17
Fragments arranged by size:
472 192 41 17 7
BtsI GCAGTG_nn'
Cuts at: 0 192 419 729
Size: 192 227 310
Fragments arranged by size:
310 227 192
EaeI y'GGCC_r
Cuts at: 0 4 26 566 729
Size: 4 22 540 163
Fragments arranged by size:
540 163 22 4
EagI C'GGCC_G
Cuts at: 0 4 729
Size: 4 725
EarI CTCTTCn'nnn_
Cuts at: 0 242 448 729
Size: 242 206 281
Fragments arranged by size:
281 242 206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 603 729
Size: 603 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 729
Size: 49 680
EcoO109I rG'GnC_Cy
Cuts at: 0 492 729
Size: 492 237
FspI TGC'GCA
Cuts at: 0 151 729
Size: 151 578
FspAI rTGC'GCAy
Cuts at: 0 151 729
Size: 151 578
HaeII r_GCGC'y
Cuts at: 0 174 729
Size: 174 555
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 554 586 729
Size: 554 32 143
Fragments arranged by size:
554 143 32
MscI TGG'CCA
Cuts at: 0 568 729
Size: 568 161
MslI CAynn'nnrTG
Cuts at: 0 88 313 729
Size: 88 225 416
Fragments arranged by size:
416 225 88
MspA1I CmG'CkG
Cuts at: 0 9 36 393 729
Size: 9 27 357 336
Fragments arranged by size:
357 336 27 9
NaeI GCC'GGC
Cuts at: 0 586 729
Size: 586 143
NcoI C'CATG_G
Cuts at: 0 48 712 729
Size: 48 664 17
Fragments arranged by size:
664 48 17
NgoMIV G'CCGG_C
Cuts at: 0 584 729
Size: 584 145
NotI GC'GGCC_GC
Cuts at: 0 4 729
Size: 4 725
NspI r_CATG'y
Cuts at: 0 150 729
Size: 150 579
PasI CC'CwG_GG
Cuts at: 0 558 729
Size: 558 171
PspOMI G'GGCC_C
Cuts at: 0 492 729
Size: 492 237
PvuII CAG'CTG
Cuts at: 0 36 393 729
Size: 36 357 336
Fragments arranged by size:
357 336 36
SacII CC_GC'GG
Cuts at: 0 10 729
Size: 10 719
SfcI C'TryA_G
Cuts at: 0 675 729
Size: 675 54
SmlI C'TyrA_G
Cuts at: 0 448 623 729
Size: 448 175 106
Fragments arranged by size:
448 175 106
StyI C'CwwG_G
Cuts at: 0 48 516 712 729
Size: 48 468 196 17
Fragments arranged by size:
468 196 48 17
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 664 729
Size: 664 65
Enzymes that do cut:
AleI AlfI AlwNI ApaI AvaI BamHI BanI BanII
BbsI BcgI BciVI Bme1580I BpmI BpuEI BsaI BsaHI
BseRI BseYI BsiEI Bsp1286I BspHI BsrFI BssSI BstEII
BstXI BstYI BtgI BtsI EaeI EagI EarI EciI
Eco57MI EcoO109I FspI FspAI HaeII Hin4I MscI MslI
MspA1I NaeI NcoI NgoMIV NotI NspI PasI PspOMI
PvuII SacII SfcI SmlI StyI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BbvCI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BsaAI
BsaBI BsaWI BsaXI BsgI BsiHKAI BsiWI BsmI BsmBI
BspEI BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstBI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EcoICRI EcoNI EcoRI EcoRV FalI FseI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NarI NdeI
NheI NruI NsiI PacI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI