Restriction map for
pF1KE2364
(Linear) (Six-base) MAPSORT of: KIEE2364.seq Check: 3674 from: 1 to: 705
KIEE2364.orf
With 182 enzymes: SgfI *
August 4, 2011 11:52 ..
AatII G_ACGT'C
Cuts at: 0 154 705
Size: 154 551
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 357 705
Size: 142 215 348
Fragments arranged by size:
348 215 142
AflIII A'CryG_T
Cuts at: 0 636 705
Size: 636 69
AlwNI CAG_nnn'CTG
Cuts at: 0 135 468 705
Size: 135 333 237
Fragments arranged by size:
333 237 135
ApaLI G'TGCA_C
Cuts at: 0 514 705
Size: 514 191
AvaI C'yCGr_G
Cuts at: 0 267 705
Size: 267 438
BanI G'GyrC_C
Cuts at: 0 184 444 558 705
Size: 184 260 114 147
Fragments arranged by size:
260 184 147 114
BbeI G_GCGC'C
Cuts at: 0 188 448 562 705
Size: 188 260 114 143
Fragments arranged by size:
260 188 143 114
BbsI GAAGACnn'nnnn_
Cuts at: 0 384 639 705
Size: 384 255 66
Fragments arranged by size:
384 255 66
Bme1580I G_kGCm'C
Cuts at: 0 518 705
Size: 518 187
BmgBI CAC'GTC
Cuts at: 0 637 705
Size: 637 68
Bpu10I CC'TnA_GC
Cuts at: 0 304 352 705
Size: 304 48 353
Fragments arranged by size:
353 304 48
BsaHI Gr'CG_yC
Cuts at: 0 151 185 418 445 559 705
Size: 151 34 233 27 114 146
Fragments arranged by size:
233 151 146 114 34 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 109 139 705
Size: 109 30 566
Fragments arranged by size:
566 109 30
BseYI C'CCAG_C
Cuts at: 0 257 682 705
Size: 257 425 23
Fragments arranged by size:
425 257 23
BsiEI CG_ry'CG
Cuts at: 0 242 705
Size: 242 463
BsiHKAI G_wGCw'C
Cuts at: 0 518 705
Size: 518 187
Bsp1286I G_dGCh'C
Cuts at: 0 518 705
Size: 518 187
BsrFI r'CCGG_y
Cuts at: 0 387 441 491 705
Size: 387 54 50 214
Fragments arranged by size:
387 214 54 50
BssSI C'ACGA_G
Cuts at: 0 162 519 705
Size: 162 357 186
Fragments arranged by size:
357 186 162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 438 705
Size: 438 267
BtsI GCAGTG_nn'
Cuts at: 0 518 705
Size: 518 187
DraIII CAC_nnn'GTG
Cuts at: 0 384 705
Size: 384 321
DrdI GACnn_nn'nnGTC
Cuts at: 0 292 705
Size: 292 413
EaeI y'GGCC_r
Cuts at: 0 239 493 566 705
Size: 239 254 73 139
Fragments arranged by size:
254 239 139 73
EagI C'GGCC_G
Cuts at: 0 239 705
Size: 239 466
EarI CTCTTCn'nnn_
Cuts at: 0 175 705
Size: 175 530
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 304 705
Size: 304 401
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 357 705
Size: 142 215 348
Fragments arranged by size:
348 215 142
EcoO109I rG'GnC_Cy
Cuts at: 0 582 705
Size: 582 123
EcoRV GAT'ATC
Cuts at: 0 546 705
Size: 546 159
HaeII r_GCGC'y
Cuts at: 0 188 427 448 562 705
Size: 188 239 21 114 143
Fragments arranged by size:
239 188 143 114 21
KasI G'GCGC_C
Cuts at: 0 184 444 558 705
Size: 184 260 114 147
Fragments arranged by size:
260 184 147 114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 705
Size: 167 538
MspA1I CmG'CkG
Cuts at: 0 33 84 261 499 705
Size: 33 51 177 238 206
Fragments arranged by size:
238 206 177 51 33
NaeI GCC'GGC
Cuts at: 0 493 705
Size: 493 212
NarI GG'CG_CC
Cuts at: 0 185 445 559 705
Size: 185 260 114 146
Fragments arranged by size:
260 185 146 114
NgoMIV G'CCGG_C
Cuts at: 0 491 705
Size: 491 214
PpuMI rG'GwC_Cy
Cuts at: 0 582 705
Size: 582 123
SapI GCTCTTCn'nnn_
Cuts at: 0 175 705
Size: 175 530
SfoI GGC'GCC
Cuts at: 0 186 446 560 705
Size: 186 260 114 145
Fragments arranged by size:
260 186 145 114
SgrAI Cr'CCGG_yG
Cuts at: 0 441 705
Size: 441 264
StyI C'CwwG_G
Cuts at: 0 233 563 705
Size: 233 330 142
Fragments arranged by size:
330 233 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 271 705
Size: 271 434
TatI w'GTAC_w
Cuts at: 0 368 705
Size: 368 337
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 109 141 705
Size: 109 32 564
Fragments arranged by size:
564 109 32
ZraI GAC'GTC
Cuts at: 0 152 705
Size: 152 553
Enzymes that do cut:
AatII AcuI AflIII AlwNI ApaLI AvaI BanI BbeI
BbsI Bme1580I BmgBI Bpu10I BsaHI BsaXI BseYI BsiEI
BsiHKAI Bsp1286I BsrFI BssSI BtgZI BtsI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRV HaeII
KasI MmeI MspA1I NaeI NarI NgoMIV PpuMI SapI
SfoI SgrAI StyI TaqII TatI TstI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaWI BseRI BsgI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI ClaI CspCI DraI EcoICRI EcoNI EcoRI FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MscI MslI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SgfI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaI XmnI