Restriction map for
pF1KE2351
(Linear) (Six-base) MAPSORT of: KIEE2351.seq Check: 2070 from: 1 to: 1404
KIEE2351.orf
With 182 enzymes: SgfI *
June 2, 2011 17:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 383 442 467 542 899 1192 1255
Size: 262 121 59 25 75 357 293 63
Cuts at: 1255 1362 1404
Size: 107 42
Fragments arranged by size:
357 293 262 121 107 75 63 59
42 25
AfeI AGC'GCT
Cuts at: 0 559 1404
Size: 559 845
AflII C'TTAA_G
Cuts at: 0 901 1404
Size: 901 503
AhdI GACnn_n'nnGTC
Cuts at: 0 513 1404
Size: 513 891
AleI CACnn'nnGTG
Cuts at: 0 99 1404
Size: 99 1305
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 117 793 803 1404
Size: 117 676 10 601
Fragments arranged by size:
676 601 117 10
ApoI r'AATT_y
Cuts at: 0 847 869 1001 1080 1294 1346 1404
Size: 847 22 132 79 214 52 58
Fragments arranged by size:
847 214 132 79 58 52 22
AseI AT'TA_AT
Cuts at: 0 773 1404
Size: 773 631
AvaI C'yCGr_G
Cuts at: 0 57 1404
Size: 57 1347
BanI G'GyrC_C
Cuts at: 0 37 1404
Size: 37 1367
BbeI G_GCGC'C
Cuts at: 0 41 1404
Size: 41 1363
BglI GCCn_nnn'nGGC
Cuts at: 0 35 1404
Size: 35 1369
BglII A'GATC_T
Cuts at: 0 1238 1404
Size: 1238 166
BlpI GC'TnA_GC
Cuts at: 0 52 1404
Size: 52 1352
Bme1580I G_kGCm'C
Cuts at: 0 1228 1404
Size: 1228 176
Bpu10I CC'TnA_GC
Cuts at: 0 554 1404
Size: 554 850
BsaI GGTCTCn'nnnn_
Cuts at: 0 721 802 1404
Size: 721 81 602
Fragments arranged by size:
721 602 81
BsaHI Gr'CG_yC
Cuts at: 0 38 1404
Size: 38 1366
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 1404
Size: 85 1319
BseYI C'CCAG_C
Cuts at: 0 11 1093 1404
Size: 11 1082 311
Fragments arranged by size:
1082 311 11
BsmI GAATG_Cn'
Cuts at: 0 736 1404
Size: 736 668
Bsp1286I G_dGCh'C
Cuts at: 0 1228 1404
Size: 1228 176
BspHI T'CATG_A
Cuts at: 0 1044 1404
Size: 1044 360
BstXI CCAn_nnnn'nTGG
Cuts at: 0 118 587 1404
Size: 118 469 817
Fragments arranged by size:
817 469 118
BstYI r'GATC_y
Cuts at: 0 1238 1404
Size: 1238 166
BtgI C'CryG_G
Cuts at: 0 6 63 1404
Size: 6 57 1341
Fragments arranged by size:
1341 57 6
BtsI GCAGTG_nn'
Cuts at: 0 422 1226 1404
Size: 422 804 178
Fragments arranged by size:
804 422 178
DraI TTT'AAA
Cuts at: 0 653 1185 1404
Size: 653 532 219
Fragments arranged by size:
653 532 219
DrdI GACnn_nn'nnGTC
Cuts at: 0 298 1404
Size: 298 1106
EarI CTCTTCn'nnn_
Cuts at: 0 238 680 1404
Size: 238 442 724
Fragments arranged by size:
724 442 238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 383 442 467 542 899 1192 1255
Size: 262 121 59 25 75 357 293 63
Cuts at: 1255 1362 1404
Size: 107 42
Fragments arranged by size:
357 293 262 121 107 75 63 59
42 25
EcoRI G'AATT_C
Cuts at: 0 1080 1294 1404
Size: 1080 214 110
Fragments arranged by size:
1080 214 110
EcoRV GAT'ATC
Cuts at: 0 796 1404
Size: 796 608
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1146 1404
Size: 1146 258
HaeII r_GCGC'y
Cuts at: 0 41 561 972 1404
Size: 41 520 411 432
Fragments arranged by size:
520 432 411 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 237 269 499 531 1404
Size: 237 32 230 32 873
Fragments arranged by size:
873 237 230 32 32
HindIII A'AGCT_T
Cuts at: 0 1204 1404
Size: 1204 200
KasI G'GCGC_C
Cuts at: 0 37 1404
Size: 37 1367
MslI CAynn'nnrTG
Cuts at: 0 99 775 785 1073 1404
Size: 99 676 10 288 331
Fragments arranged by size:
676 331 288 99 10
MspA1I CmG'CkG
Cuts at: 0 8 91 1404
Size: 8 83 1313
Fragments arranged by size:
1313 83 8
NarI GG'CG_CC
Cuts at: 0 38 1404
Size: 38 1366
NspI r_CATG'y
Cuts at: 0 766 1404
Size: 766 638
PflMI CCAn_nnn'nTGG
Cuts at: 0 1279 1404
Size: 1279 125
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 682 714 1404
Size: 682 32 690
Fragments arranged by size:
690 682 32
SacII CC_GC'GG
Cuts at: 0 9 1404
Size: 9 1395
SexAI A'CCwGG_T
Cuts at: 0 811 1404
Size: 811 593
SfcI C'TryA_G
Cuts at: 0 174 741 1404
Size: 174 567 663
Fragments arranged by size:
663 567 174
SfoI GGC'GCC
Cuts at: 0 39 1404
Size: 39 1365
SmlI C'TyrA_G
Cuts at: 0 901 1404
Size: 901 503
SspI AAT'ATT
Cuts at: 0 1152 1398 1404
Size: 1152 246 6
Fragments arranged by size:
1152 246 6
XmnI GAAnn'nnTTC
Cuts at: 0 1040 1152 1347 1404
Size: 1040 112 195 57
Fragments arranged by size:
1040 195 112 57
Enzymes that do cut:
AcuI AfeI AflII AhdI AleI AlfI ApoI AseI
AvaI BanI BbeI BglI BglII BlpI Bme1580I Bpu10I
BsaI BsaHI BseRI BseYI BsmI Bsp1286I BspHI BstXI
BstYI BtgI BtsI DraI DrdI EarI Eco57MI EcoRI
EcoRV FalI HaeII Hin4I HindIII KasI MslI MspA1I
NarI NspI PflMI PsrI SacII SexAI SfcI SfoI
SmlI SspI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AloI
AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BmgBI
BmrI BmtI BplI BpmI BpuEI BsaAI BsaBI BsaWI
BsaXI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EaeI
EagI EciI EcoICRI EcoNI EcoO109I FseI FspI FspAI
HincII HpaI KpnI MfeI MluI MmeI MscI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI