Restriction map for
pF1KE2348
(Linear) (Six-base) MAPSORT of: KIEE2348.seq Check: 9210 from: 1 to: 4311
KIEE2348.orf
With 182 enzymes: SgfI *
June 2, 2011 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1721 3531 4311
Size: 1721 1810 780
Fragments arranged by size:
1810 1721 780
AccI GT'mk_AC
Cuts at: 0 619 2543 4311
Size: 619 1924 1768
Fragments arranged by size:
1924 1768 619
Acc65I G'GTAC_C
Cuts at: 0 3602 4311
Size: 3602 709
AclI AA'CG_TT
Cuts at: 0 2987 4311
Size: 2987 1324
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 564 2607 3136 3905 3983 4311
Size: 283 281 2043 529 769 78 328
Fragments arranged by size:
2043 769 529 328 283 281 78
AflII C'TTAA_G
Cuts at: 0 787 4311
Size: 787 3524
AflIII A'CryG_T
Cuts at: 0 395 1059 3274 4311
Size: 395 664 2215 1037
Fragments arranged by size:
2215 1037 664 395
AgeI A'CCGG_T
Cuts at: 0 736 4311
Size: 736 3575
AhdI GACnn_n'nnGTC
Cuts at: 0 399 4311
Size: 399 3912
AleI CACnn'nnGTG
Cuts at: 0 1306 2674 3475 4311
Size: 1306 1368 801 836
Fragments arranged by size:
1368 1306 836 801
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2756 4311
Size: 2756 1555
AlwNI CAG_nnn'CTG
Cuts at: 0 490 1387 1982 2238 2630 3237 4311
Size: 490 897 595 256 392 607 1074
Fragments arranged by size:
1074 897 607 595 490 392 256
ApaI G_GGCC'C
Cuts at: 0 2566 3059 4311
Size: 2566 493 1252
Fragments arranged by size:
2566 1252 493
ApoI r'AATT_y
Cuts at: 0 1096 2313 3858 4311
Size: 1096 1217 1545 453
Fragments arranged by size:
1545 1217 1096 453
AvaI C'yCGr_G
Cuts at: 0 161 2087 2681 4311
Size: 161 1926 594 1630
Fragments arranged by size:
1926 1630 594 161
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1497 1530 4311
Size: 1497 33 2781
Fragments arranged by size:
2781 1497 33
BamHI G'GATC_C
Cuts at: 0 1898 2861 4311
Size: 1898 963 1450
Fragments arranged by size:
1898 1450 963
BanI G'GyrC_C
Cuts at: 0 2293 3602 4311
Size: 2293 1309 709
Fragments arranged by size:
2293 1309 709
BanII G_rGCy'C
Cuts at: 0 383 827 2566 3059 4029 4311
Size: 383 444 1739 493 970 282
Fragments arranged by size:
1739 970 493 444 383 282
BbsI GAAGACnn'nnnn_
Cuts at: 0 398 413 447 1308 3021 3893 4311
Size: 398 15 34 861 1713 872 418
Fragments arranged by size:
1713 872 861 418 398 34 15
BciVI GTATCCnnnnn_n'
Cuts at: 0 1525 3648 4311
Size: 1525 2123 663
Fragments arranged by size:
2123 1525 663
BclI T'GATC_A
Cuts at: 0 560 4311
Size: 560 3751
BfrBI ATG'CAT
Cuts at: 0 195 4311
Size: 195 4116
BglI GCCn_nnn'nGGC
Cuts at: 0 361 1384 1489 1891 4311
Size: 361 1023 105 402 2420
Fragments arranged by size:
2420 1023 402 361 105
BlpI GC'TnA_GC
Cuts at: 0 3938 4311
Size: 3938 373
Bme1580I G_kGCm'C
Cuts at: 0 2525 2566 2818 3059 4311
Size: 2525 41 252 241 1252
Fragments arranged by size:
2525 1252 252 241 41
BmgBI CAC'GTC
Cuts at: 0 396 429 2949 3164 4311
Size: 396 33 2520 215 1147
Fragments arranged by size:
2520 1147 396 215 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 32 292 1664 4311
Size: 32 260 1372 2647
Fragments arranged by size:
2647 1372 260 32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 192 3631 4048 4311
Size: 192 3439 417 263
Fragments arranged by size:
3439 417 263 192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 914 1494 1768 3078 3761 3928 4311
Size: 914 580 274 1310 683 167 383
Fragments arranged by size:
1310 914 683 580 383 274 167
Bpu10I CC'TnA_GC
Cuts at: 0 2053 4311
Size: 2053 2258
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 2179 3806 4311
Size: 273 1906 1627 505
Fragments arranged by size:
1906 1627 505 273
BsaI GGTCTCn'nnnn_
Cuts at: 0 86 407 2686 3394 4102 4311
Size: 86 321 2279 708 708 209
Fragments arranged by size:
2279 708 708 321 209 86
BsaAI yAC'GTr
Cuts at: 0 3275 4311
Size: 3275 1036
BsaBI GATnn'nnATC
Cuts at: 0 2977 4311
Size: 2977 1334
BsaWI w'CCGG_w
Cuts at: 0 272 736 2183 4311
Size: 272 464 1447 2128
Fragments arranged by size:
2128 1447 464 272
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 823 853 887 917 2743 2773 3076 3106
Size: 823 30 34 30 1826 30 303 30
Cuts at: 3106 4311
Size: 1205
Fragments arranged by size:
1826 1205 823 303 34 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 235 664 1171 1174 1491 1636 2057 2810
Size: 235 429 507 3 317 145 421 753
Cuts at: 2810 4311
Size: 1501
Fragments arranged by size:
1501 753 507 429 421 317 235 145
3
BseYI C'CCAG_C
Cuts at: 0 310 666 990 1886 2011 2355 2557 3375
Size: 310 356 324 896 125 344 202 818
Cuts at: 3375 3705 4311
Size: 330 606
Fragments arranged by size:
896 818 606 356 344 330 324 310
202 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1717 2512 3067 3205 4147 4311
Size: 1717 795 555 138 942 164
Fragments arranged by size:
1717 942 795 555 164 138
BsiEI CG_ry'CG
Cuts at: 0 127 4311
Size: 127 4184
BsiHKAI G_wGCw'C
Cuts at: 0 261 383 827 4311
Size: 261 122 444 3484
Fragments arranged by size:
3484 444 261 122
BsmI GAATG_Cn'
Cuts at: 0 139 195 2572 4311
Size: 139 56 2377 1739
Fragments arranged by size:
2377 1739 139 56
Bsp1286I G_dGCh'C
Cuts at: 0 261 383 827 2525 2566 2818 3059 4029
Size: 261 122 444 1698 41 252 241 970
Cuts at: 4029 4311
Size: 282
Fragments arranged by size:
1698 970 444 282 261 252 241 122
41
BspEI T'CCGG_A
Cuts at: 0 272 2183 4311
Size: 272 1911 2128
Fragments arranged by size:
2128 1911 272
BspHI T'CATG_A
Cuts at: 0 2264 4311
Size: 2264 2047
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 650 1721 3531 4311
Size: 650 1071 1810 780
Fragments arranged by size:
1810 1071 780 650
BsrBI CCG'CTC
Cuts at: 0 1665 2057 4311
Size: 1665 392 2254
Fragments arranged by size:
2254 1665 392
BsrDI GCAATG_nn'
Cuts at: 0 1001 1853 4148 4311
Size: 1001 852 2295 163
Fragments arranged by size:
2295 1001 852 163
BsrFI r'CCGG_y
Cuts at: 0 736 3828 4311
Size: 736 3092 483
Fragments arranged by size:
3092 736 483
BssSI C'ACGA_G
Cuts at: 0 415 4311
Size: 415 3896
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2490 4311
Size: 2490 1821
BstBI TT'CG_AA
Cuts at: 0 2868 4311
Size: 2868 1443
BstEII G'GTnAC_C
Cuts at: 0 1245 3576 4311
Size: 1245 2331 735
Fragments arranged by size:
2331 1245 735
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1227 2557 2934 4311
Size: 1227 1330 377 1377
Fragments arranged by size:
1377 1330 1227 377
BstYI r'GATC_y
Cuts at: 0 515 782 1751 1898 2861 4311
Size: 515 267 969 147 963 1450
Fragments arranged by size:
1450 969 963 515 267 147
BstZ17I GTA'TAC
Cuts at: 0 620 4311
Size: 620 3691
Bsu36I CC'TnA_GG
Cuts at: 0 1987 4311
Size: 1987 2324
BtgI C'CryG_G
Cuts at: 0 767 1049 2762 3034 3335 4076 4311
Size: 767 282 1713 272 301 741 235
Fragments arranged by size:
1713 767 741 301 282 272 235
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3295 3303 4141 4311
Size: 3295 8 838 170
Fragments arranged by size:
3295 838 170 8
BtsI GCAGTG_nn'
Cuts at: 0 1729 1791 3056 4311
Size: 1729 62 1265 1255
Fragments arranged by size:
1729 1265 1255 62
ClaI AT'CG_AT
Cuts at: 0 1742 4311
Size: 1742 2569
DraI TTT'AAA
Cuts at: 0 2318 4311
Size: 2318 1993
DraIII CAC_nnn'GTG
Cuts at: 0 300 2633 4311
Size: 300 2333 1678
Fragments arranged by size:
2333 1678 300
EaeI y'GGCC_r
Cuts at: 0 1043 2000 4311
Size: 1043 957 2311
Fragments arranged by size:
2311 1043 957
EarI CTCTTCn'nnn_
Cuts at: 0 458 1415 1673 2495 3724 4311
Size: 458 957 258 822 1229 587
Fragments arranged by size:
1229 957 822 587 458 258
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3696 4024 4311
Size: 3696 328 287
Fragments arranged by size:
3696 328 287
EcoICRI GAG'CTC
Cuts at: 0 381 825 4311
Size: 381 444 3486
Fragments arranged by size:
3486 444 381
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 564 914 1494 1768 2607 3078 3136
Size: 283 281 350 580 274 839 471 58
Cuts at: 3136 3761 3905 3928 3983 4311
Size: 625 144 23 55 328
Fragments arranged by size:
839 625 580 471 350 328 283 281
274 144 58 55 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 217 2438 4311
Size: 217 2221 1873
Fragments arranged by size:
2221 1873 217
EcoO109I rG'GnC_Cy
Cuts at: 0 171 898 2431 2562 2563 3055 3056 3099
Size: 171 727 1533 131 1 492 1 43
Cuts at: 3099 3177 4311
Size: 78 1134
Fragments arranged by size:
1533 1134 727 492 171 131 78 43
1 1
EcoRV GAT'ATC
Cuts at: 0 9 4311
Size: 9 4302
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2503 4311
Size: 2503 1808
HaeII r_GCGC'y
Cuts at: 0 2070 4311
Size: 2070 2241
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 385 417 1910 1942 2786 2818 3447 3474
Size: 385 32 1493 32 844 32 629 27
Cuts at: 3474 3479 3506 3734 3755 3766 3787 3973 4005
Size: 5 27 228 21 11 21 186 32
Cuts at: 4005 4216 4248 4311
Size: 211 32 63
Fragments arranged by size:
1493 844 629 385 228 211 186 63
32 32 32 32 32 27 27 21
21 11 5
HincII GTy'rAC
Cuts at: 0 1392 2244 2946 3416 4311
Size: 1392 852 702 470 895
Fragments arranged by size:
1392 895 852 702 470
HpaI GTT'AAC
Cuts at: 0 3416 4311
Size: 3416 895
KpnI G_GTAC'C
Cuts at: 0 3606 4311
Size: 3606 705
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 866 1147 1752 4271 4311
Size: 110 756 281 605 2519 40
Fragments arranged by size:
2519 756 605 281 110 40
MscI TGG'CCA
Cuts at: 0 2002 4311
Size: 2002 2309
MslI CAynn'nnrTG
Cuts at: 0 1204 1225 1306 2674 3475 4311
Size: 1204 21 81 1368 801 836
Fragments arranged by size:
1368 1204 836 801 81 21
MspA1I CmG'CkG
Cuts at: 0 570 970 1596 1979 2496 4072 4311
Size: 570 400 626 383 517 1576 239
Fragments arranged by size:
1576 626 570 517 400 383 239
NcoI C'CATG_G
Cuts at: 0 767 2762 4076 4311
Size: 767 1995 1314 235
Fragments arranged by size:
1995 1314 767 235
NdeI CA'TA_TG
Cuts at: 0 1262 2729 4311
Size: 1262 1467 1582
Fragments arranged by size:
1582 1467 1262
NsiI A_TGCA'T
Cuts at: 0 197 4311
Size: 197 4114
PasI CC'CwG_GG
Cuts at: 0 816 1269 4203 4311
Size: 816 453 2934 108
Fragments arranged by size:
2934 816 453 108
PfoI T'CCnGG_A
Cuts at: 0 212 1649 2120 4311
Size: 212 1437 471 2191
Fragments arranged by size:
2191 1437 471 212
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2680 2712 3992 4024 4311
Size: 2680 32 1280 32 287
Fragments arranged by size:
2680 1280 287 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 898 2431 3099 4311
Size: 898 1533 668 1212
Fragments arranged by size:
1533 1212 898 668
PshAI GACnn'nnGTC
Cuts at: 0 2429 4311
Size: 2429 1882
PsiI TTA'TAA
Cuts at: 0 929 4311
Size: 929 3382
PspOMI G'GGCC_C
Cuts at: 0 2562 3055 4311
Size: 2562 493 1256
Fragments arranged by size:
2562 1256 493
PspXI vC'TCGA_Gb
Cuts at: 0 2681 4311
Size: 2681 1630
PstI C_TGCA'G
Cuts at: 0 439 647 1601 4287 4311
Size: 439 208 954 2686 24
Fragments arranged by size:
2686 954 439 208 24
PvuII CAG'CTG
Cuts at: 0 570 970 1596 1979 2496 4072 4311
Size: 570 400 626 383 517 1576 239
Fragments arranged by size:
1576 626 570 517 400 383 239
SacI G_AGCT'C
Cuts at: 0 383 827 4311
Size: 383 444 3484
Fragments arranged by size:
3484 444 383
SapI GCTCTTCn'nnn_
Cuts at: 0 458 4311
Size: 458 3853
ScaI AGT'ACT
Cuts at: 0 2737 4311
Size: 2737 1574
SexAI A'CCwGG_T
Cuts at: 0 2237 2629 4311
Size: 2237 392 1682
Fragments arranged by size:
2237 1682 392
SfcI C'TryA_G
Cuts at: 0 435 643 1597 4283 4311
Size: 435 208 954 2686 28
Fragments arranged by size:
2686 954 435 208 28
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 361 1891 4311
Size: 361 1530 2420
Fragments arranged by size:
2420 1530 361
SmaI CCC'GGG
Cuts at: 0 2089 4311
Size: 2089 2222
SmlI C'TyrA_G
Cuts at: 0 252 787 2194 2681 3821 4311
Size: 252 535 1407 487 1140 490
Fragments arranged by size:
1407 1140 535 490 487 252
SrfI GCCC'GGGC
Cuts at: 0 2089 4311
Size: 2089 2222
StuI AGG'CCT
Cuts at: 0 1894 1992 4311
Size: 1894 98 2319
Fragments arranged by size:
2319 1894 98
StyI C'CwwG_G
Cuts at: 0 146 230 767 1487 2567 2762 3512 4076
Size: 146 84 537 720 1080 195 750 564
Cuts at: 4076 4311
Size: 235
Fragments arranged by size:
1080 750 720 564 537 235 195 146
84
SwaI ATTT'AAAT
Cuts at: 0 2318 4311
Size: 2318 1993
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 311 808 1198 2311 4224 4311
Size: 311 497 390 1113 1913 87
Fragments arranged by size:
1913 1113 497 390 311 87
TatI w'GTAC_w
Cuts at: 0 647 2735 3878 4311
Size: 647 2088 1143 433
Fragments arranged by size:
2088 1143 647 433
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 382 414 3076 3108 3148 3180 4199 4231
Size: 382 32 2662 32 40 32 1019 32
Cuts at: 4231 4311
Size: 80
Fragments arranged by size:
2662 1019 382 80 40 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 3507 4311
Size: 3507 804
XbaI T'CTAG_A
Cuts at: 0 1842 4311
Size: 1842 2469
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 765 4074 4311
Size: 765 3309 237
Fragments arranged by size:
3309 765 237
XhoI C'TCGA_G
Cuts at: 0 2681 4311
Size: 2681 1630
XmaI C'CCGG_G
Cuts at: 0 2087 4311
Size: 2087 2224
XmnI GAAnn'nnTTC
Cuts at: 0 1424 4311
Size: 1424 2887
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApoI AvaI BaeI
BamHI BanI BanII BbsI BciVI BclI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FalI HaeII Hin4I HincII HpaI KpnI MmeI MscI
MslI MspA1I NcoI NdeI NsiI PasI PfoI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuII SacI
SapI ScaI SexAI SfcI SfiI SmaI SmlI SrfI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AloI ApaLI AscI AseI AsiSI AvrII
BbeI BbvCI BcgI BglII BmtI BsaHI BsiWI BsmBI
BsrGI BssHII CspCI DrdI EagI EcoRI FseI FspI
FspAI HindIII KasI MfeI MluI NaeI NarI NgoMIV
NheI NotI NruI NspI PacI PciI PflMI PmeI
PmlI PsrI PvuI RsrII SacII SalI SanDI SbfI
SfoI SgfI SgrAI SnaBI SpeI SphI SspI ZraI