Restriction map for
pF1KE2342
(Linear) (Six-base) MAPSORT of: KIEE2342.seq Check: 2170 from: 1 to: 1269
KIEE2342.orf
With 182 enzymes: SgfI *
March 29, 2011 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1063 1269
Size: 1063 206
AatII G_ACGT'C
Cuts at: 0 611 1269
Size: 611 658
AccI GT'mk_AC
Cuts at: 0 818 1019 1269
Size: 818 201 250
Fragments arranged by size:
818 250 201
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1269
Size: 154 1115
AflIII A'CryG_T
Cuts at: 0 983 1269
Size: 983 286
AlwNI CAG_nnn'CTG
Cuts at: 0 283 511 1269
Size: 283 228 758
Fragments arranged by size:
758 283 228
ApaI G_GGCC'C
Cuts at: 0 87 1269
Size: 87 1182
ApoI r'AATT_y
Cuts at: 0 235 1269
Size: 235 1034
AvaI C'yCGr_G
Cuts at: 0 347 656 1269
Size: 347 309 613
Fragments arranged by size:
613 347 309
BanI G'GyrC_C
Cuts at: 0 379 1269
Size: 379 890
BanII G_rGCy'C
Cuts at: 0 87 371 483 500 742 881 1269
Size: 87 284 112 17 242 139 388
Fragments arranged by size:
388 284 242 139 112 87 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 773 1269
Size: 773 496
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 34 1269
Size: 34 1235
BclI T'GATC_A
Cuts at: 0 458 1269
Size: 458 811
BglI GCCn_nnn'nGGC
Cuts at: 0 190 876 1269
Size: 190 686 393
Fragments arranged by size:
686 393 190
BglII A'GATC_T
Cuts at: 0 633 1269
Size: 633 636
BlpI GC'TnA_GC
Cuts at: 0 124 1269
Size: 124 1145
Bme1580I G_kGCm'C
Cuts at: 0 87 384 1269
Size: 87 297 885
Fragments arranged by size:
885 297 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 337 865 1269
Size: 63 274 528 404
Fragments arranged by size:
528 404 274 63
Bpu10I CC'TnA_GC
Cuts at: 0 1061 1269
Size: 1061 208
BsaAI yAC'GTr
Cuts at: 0 822 921 1269
Size: 822 99 348
Fragments arranged by size:
822 348 99
BsaHI Gr'CG_yC
Cuts at: 0 608 1269
Size: 608 661
BsaWI w'CCGG_w
Cuts at: 0 355 1269
Size: 355 914
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 352 1269
Size: 145 207 917
Fragments arranged by size:
917 207 145
BseYI C'CCAG_C
Cuts at: 0 21 77 249 726 1210 1269
Size: 21 56 172 477 484 59
Fragments arranged by size:
484 477 172 59 56 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 1091 1269
Size: 901 190 178
Fragments arranged by size:
901 190 178
BsiEI CG_ry'CG
Cuts at: 0 55 207 1269
Size: 55 152 1062
Fragments arranged by size:
1062 152 55
BsiHKAI G_wGCw'C
Cuts at: 0 179 1269
Size: 179 1090
BsmI GAATG_Cn'
Cuts at: 0 1124 1269
Size: 1124 145
Bsp1286I G_dGCh'C
Cuts at: 0 87 179 371 384 483 500 742 881
Size: 87 92 192 13 99 17 242 139
Cuts at: 881 1269
Size: 388
Fragments arranged by size:
388 242 192 139 99 92 87 17
13
BspEI T'CCGG_A
Cuts at: 0 355 1269
Size: 355 914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 236 923 1063 1269
Size: 236 687 140 206
Fragments arranged by size:
687 236 206 140
BsrBI CCG'CTC
Cuts at: 0 849 1269
Size: 849 420
BsrDI GCAATG_nn'
Cuts at: 0 219 423 1050 1149 1269
Size: 219 204 627 99 120
Fragments arranged by size:
627 219 204 120 99
BsrFI r'CCGG_y
Cuts at: 0 64 1269
Size: 64 1205
BsrGI T'GTAC_A
Cuts at: 0 986 1269
Size: 986 283
BstXI CCAn_nnnn'nTGG
Cuts at: 0 795 1269
Size: 795 474
BstYI r'GATC_y
Cuts at: 0 633 1269
Size: 633 636
BtgI C'CryG_G
Cuts at: 0 101 155 1166 1269
Size: 101 54 1011 103
Fragments arranged by size:
1011 103 101 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1055 1269
Size: 1055 214
BtsI GCAGTG_nn'
Cuts at: 0 368 1055 1269
Size: 368 687 214
Fragments arranged by size:
687 368 214
ClaI AT'CG_AT
Cuts at: 0 203 1269
Size: 203 1066
EaeI y'GGCC_r
Cuts at: 0 52 485 860 936 948 1269
Size: 52 433 375 76 12 321
Fragments arranged by size:
433 375 321 76 52 12
EagI C'GGCC_G
Cuts at: 0 52 1269
Size: 52 1217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 154 337 865 1269
Size: 63 91 183 528 404
Fragments arranged by size:
528 404 183 91 63
EcoO109I rG'GnC_Cy
Cuts at: 0 868 1269
Size: 868 401
EcoRV GAT'ATC
Cuts at: 0 344 1269
Size: 344 925
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 328 360 505 537 1269
Size: 29 299 32 145 32 732
Fragments arranged by size:
732 299 145 32 32 29
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 1269
Size: 413 856
MscI TGG'CCA
Cuts at: 0 487 938 1269
Size: 487 451 331
Fragments arranged by size:
487 451 331
MspA1I CmG'CkG
Cuts at: 0 81 249 1093 1269
Size: 81 168 844 176
Fragments arranged by size:
844 176 168 81
NaeI GCC'GGC
Cuts at: 0 66 1269
Size: 66 1203
NcoI C'CATG_G
Cuts at: 0 155 1166 1269
Size: 155 1011 103
Fragments arranged by size:
1011 155 103
NgoMIV G'CCGG_C
Cuts at: 0 64 1269
Size: 64 1205
NotI GC'GGCC_GC
Cuts at: 0 52 1269
Size: 52 1217
NspI r_CATG'y
Cuts at: 0 325 987 1269
Size: 325 662 282
Fragments arranged by size:
662 325 282
PciI A'CATG_T
Cuts at: 0 983 1269
Size: 983 286
PfoI T'CCnGG_A
Cuts at: 0 893 1269
Size: 893 376
PmlI CAC'GTG
Cuts at: 0 921 1269
Size: 921 348
PspOMI G'GGCC_C
Cuts at: 0 83 1269
Size: 83 1186
PvuI CG_AT'CG
Cuts at: 0 207 1269
Size: 207 1062
PvuII CAG'CTG
Cuts at: 0 249 1093 1269
Size: 249 844 176
Fragments arranged by size:
844 249 176
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 190 1269
Size: 190 1079
StuI AGG'CCT
Cuts at: 0 1036 1269
Size: 1036 233
StyI C'CwwG_G
Cuts at: 0 155 188 1031 1166 1269
Size: 155 33 843 135 103
Fragments arranged by size:
843 155 135 103 33
TatI w'GTAC_w
Cuts at: 0 470 986 1269
Size: 470 516 283
Fragments arranged by size:
516 470 283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 633 1269
Size: 633 636
ZraI GAC'GTC
Cuts at: 0 609 1269
Size: 609 660
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AlwNI ApaI ApoI
AvaI BanI BanII BbsI BcgI BclI BglI BglII
BlpI Bme1580I BpmI Bpu10I BsaAI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BsrGI BstXI BstYI BtgI BtgZI
BtsI ClaI EaeI EagI Eco57MI EcoO109I EcoRV Hin4I
MmeI MscI MspA1I NaeI NcoI NgoMIV NotI NspI
PciI PfoI PmlI PspOMI PvuI PvuII SfiI StuI
StyI TatI XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BciVI BfrBI BmgBI BmrI BmtI BplI
BpuEI BsaI BsaBI BsaXI BsiWI BsmBI BspHI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I CspCI DraI
DraIII DrdI EarI EciI EcoICRI EcoNI EcoRI FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MslI NarI NdeI NheI NruI
NsiI PacI PasI PflMI PmeI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TstI Tth111I XbaI XhoI XmaI XmnI