Restriction map for
pF1KE2341
(Linear) (Six-base) MAPSORT of: KIEE2341.seq Check: 8561 from: 1 to: 2208
KIEE2341.orf
With 182 enzymes: SgfI *
March 29, 2011 17:34 ..
AccI GT'mk_AC
Cuts at: 0 952 2208
Size: 952 1256
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 638 658 1817 1849 2161 2208
Size: 440 198 20 1159 32 312 47
Fragments arranged by size:
1159 440 312 198 47 32 20
ApoI r'AATT_y
Cuts at: 0 302 525 1084 1178 1748 2208
Size: 302 223 559 94 570 460
Fragments arranged by size:
570 559 460 302 223 94
BamHI G'GATC_C
Cuts at: 0 1068 1306 2208
Size: 1068 238 902
Fragments arranged by size:
1068 902 238
BanI G'GyrC_C
Cuts at: 0 654 2208
Size: 654 1554
BanII G_rGCy'C
Cuts at: 0 7 2208
Size: 7 2201
BbsI GAAGACnn'nnnn_
Cuts at: 0 256 347 502 2208
Size: 256 91 155 1706
Fragments arranged by size:
1706 256 155 91
BbvCI CC'TCA_GC
Cuts at: 0 13 2208
Size: 13 2195
BciVI GTATCCnnnnn_n'
Cuts at: 0 871 2208
Size: 871 1337
BfrBI ATG'CAT
Cuts at: 0 1801 2208
Size: 1801 407
BglII A'GATC_T
Cuts at: 0 621 824 2208
Size: 621 203 1384
Fragments arranged by size:
1384 621 203
BlpI GC'TnA_GC
Cuts at: 0 2164 2208
Size: 2164 44
BmrI ACTGGGnnnn_n'
Cuts at: 0 354 2208
Size: 354 1854
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 995 1418 1799 2208
Size: 995 423 381 409
Fragments arranged by size:
995 423 409 381
Bpu10I CC'TnA_GC
Cuts at: 0 13 156 2208
Size: 13 143 2052
Fragments arranged by size:
2052 143 13
BsaHI Gr'CG_yC
Cuts at: 0 1896 2208
Size: 1896 312
BsaWI w'CCGG_w
Cuts at: 0 1309 2208
Size: 1309 899
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 89 119 227 257 561 591 752 782
Size: 89 30 108 30 304 30 161 30
Cuts at: 782 1772 1802 2208
Size: 990 30 406
Fragments arranged by size:
990 406 304 161 108 89 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 110 706 932 2208
Size: 110 596 226 1276
Fragments arranged by size:
1276 596 226 110
BseYI C'CCAG_C
Cuts at: 0 743 2208
Size: 743 1465
BsmI GAATG_Cn'
Cuts at: 0 846 2122 2208
Size: 846 1276 86
Fragments arranged by size:
1276 846 86
Bsp1286I G_dGCh'C
Cuts at: 0 7 2208
Size: 7 2201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1561 2208
Size: 1561 647
BsrBI CCG'CTC
Cuts at: 0 750 2208
Size: 750 1458
BsrDI GCAATG_nn'
Cuts at: 0 572 2208
Size: 572 1636
BsrFI r'CCGG_y
Cuts at: 0 43 2208
Size: 43 2165
BsrGI T'GTAC_A
Cuts at: 0 1603 2208
Size: 1603 605
BssSI C'ACGA_G
Cuts at: 0 679 2208
Size: 679 1529
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1285 2044 2208
Size: 1285 759 164
Fragments arranged by size:
1285 759 164
BstEII G'GTnAC_C
Cuts at: 0 39 2208
Size: 39 2169
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1777 2208
Size: 1777 431
BstYI r'GATC_y
Cuts at: 0 621 824 1068 1306 1809 2208
Size: 621 203 244 238 503 399
Fragments arranged by size:
621 503 399 244 238 203
Bsu36I CC'TnA_GG
Cuts at: 0 797 1919 2208
Size: 797 1122 289
Fragments arranged by size:
1122 797 289
BtsI GCAGTG_nn'
Cuts at: 0 212 2208
Size: 212 1996
EaeI y'GGCC_r
Cuts at: 0 209 477 984 2208
Size: 209 268 507 1224
Fragments arranged by size:
1224 507 268 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1985 2208
Size: 1985 223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 638 658 995 1418 1799 1817 1849
Size: 440 198 20 337 423 381 18 32
Cuts at: 1849 2161 2208
Size: 312 47
Fragments arranged by size:
440 423 381 337 312 198 47 32
20 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 324 2039 2208
Size: 324 1715 169
Fragments arranged by size:
1715 324 169
EcoO109I rG'GnC_Cy
Cuts at: 0 2032 2208
Size: 2032 176
EcoRI G'AATT_C
Cuts at: 0 302 1748 2208
Size: 302 1446 460
Fragments arranged by size:
1446 460 302
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2142 2208
Size: 2142 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1046 1078 1178 1210 1291 1323 1474 1506
Size: 1046 32 100 32 81 32 151 32
Cuts at: 1506 1772 1804 2141 2173 2208
Size: 266 32 337 32 35
Fragments arranged by size:
1046 337 266 151 100 81 35 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 291 2208
Size: 291 1917
HindIII A'AGCT_T
Cuts at: 0 1861 2104 2208
Size: 1861 243 104
Fragments arranged by size:
1861 243 104
MfeI C'AATT_G
Cuts at: 0 536 2208
Size: 536 1672
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 2208
Size: 696 1512
MscI TGG'CCA
Cuts at: 0 211 479 986 2208
Size: 211 268 507 1222
Fragments arranged by size:
1222 507 268 211
MslI CAynn'nnrTG
Cuts at: 0 2089 2208
Size: 2089 119
MspA1I CmG'CkG
Cuts at: 0 12 2208
Size: 12 2196
NdeI CA'TA_TG
Cuts at: 0 1803 2208
Size: 1803 405
NsiI A_TGCA'T
Cuts at: 0 1803 2208
Size: 1803 405
PflMI CCAn_nnn'nTGG
Cuts at: 0 1825 2208
Size: 1825 383
PfoI T'CCnGG_A
Cuts at: 0 1182 1704 2208
Size: 1182 522 504
Fragments arranged by size:
1182 522 504
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 300 332 2208
Size: 300 32 1876
Fragments arranged by size:
1876 300 32
ScaI AGT'ACT
Cuts at: 0 1228 2208
Size: 1228 980
SfcI C'TryA_G
Cuts at: 0 953 1442 2208
Size: 953 489 766
Fragments arranged by size:
953 766 489
SgrAI Cr'CCGG_yG
Cuts at: 0 43 2208
Size: 43 2165
SpeI A'CTAG_T
Cuts at: 0 937 2208
Size: 937 1271
SspI AAT'ATT
Cuts at: 0 1977 2208
Size: 1977 231
StyI C'CwwG_G
Cuts at: 0 1044 1763 2028 2208
Size: 1044 719 265 180
Fragments arranged by size:
1044 719 265 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 800 1150 2208
Size: 800 350 1058
Fragments arranged by size:
1058 800 350
TatI w'GTAC_w
Cuts at: 0 1226 1473 1603 2208
Size: 1226 247 130 605
Fragments arranged by size:
1226 605 247 130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 191 223 561 593 647 679 2208
Size: 191 32 338 32 54 32 1529
Fragments arranged by size:
1529 338 191 54 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1429 1786 2208
Size: 1429 357 422
Fragments arranged by size:
1429 422 357
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 871 2208
Size: 871 1337
XmnI GAAnn'nnTTC
Cuts at: 0 2122 2208
Size: 2122 86
Enzymes that do cut:
AccI AcuI ApoI BamHI BanI BanII BbsI BbvCI
BciVI BfrBI BglII BlpI BmrI BpmI Bpu10I BsaHI
BsaWI BsaXI BseRI BseYI BsmI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtsI EaeI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI Hin4I HincII HindIII MfeI MmeI MscI MslI
MspA1I NdeI NsiI PflMI PfoI PpiI ScaI SfcI
SgrAI SpeI SspI StyI TaqII TatI TstI Tth111I
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BbeI BcgI BclI
BglI Bme1580I BmgBI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI
BssHII BstBI BstZ17I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EagI EarI EcoICRI EcoRV FseI FspI
FspAI HaeII HpaI KasI KpnI MluI NaeI NarI
NcoI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SmaI
SmlI SnaBI SphI SrfI StuI SwaI XbaI XhoI
XmaI ZraI