Restriction map for
pF1KE2340
(Linear) (Six-base) MAPSORT of: KIEE2340.seq Check: 635 from: 1 to: 510
KIEE2340.orf
With 182 enzymes: SgfI *
March 29, 2011 17:34 ..
AatII G_ACGT'C
Cuts at: 0 296 510
Size: 296 214
AccI GT'mk_AC
Cuts at: 0 421 510
Size: 421 89
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 470 510
Size: 57 413 40
Fragments arranged by size:
413 57 40
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 342 510
Size: 342 168
AvaI C'yCGr_G
Cuts at: 0 91 510
Size: 91 419
BanII G_rGCy'C
Cuts at: 0 86 510
Size: 86 424
BbsI GAAGACnn'nnnn_
Cuts at: 0 458 510
Size: 458 52
BglI GCCn_nnn'nGGC
Cuts at: 0 245 397 510
Size: 245 152 113
Fragments arranged by size:
245 152 113
BmrI ACTGGGnnnn_n'
Cuts at: 0 147 510
Size: 147 363
BmtI G_CTAG'C
Cuts at: 0 349 510
Size: 349 161
BsaHI Gr'CG_yC
Cuts at: 0 293 510
Size: 293 217
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 39 69 510
Size: 39 30 441
Fragments arranged by size:
441 39 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 157 295 510
Size: 157 138 215
Fragments arranged by size:
215 157 138
BseYI C'CCAG_C
Cuts at: 0 387 510
Size: 387 123
BsiEI CG_ry'CG
Cuts at: 0 63 510
Size: 63 447
BsiHKAI G_wGCw'C
Cuts at: 0 356 510
Size: 356 154
Bsp1286I G_dGCh'C
Cuts at: 0 86 356 510
Size: 86 270 154
Fragments arranged by size:
270 154 86
BsrFI r'CCGG_y
Cuts at: 0 10 62 374 510
Size: 10 52 312 136
Fragments arranged by size:
312 136 52 10
BstYI r'GATC_y
Cuts at: 0 336 510
Size: 336 174
BstZ17I GTA'TAC
Cuts at: 0 422 510
Size: 422 88
BtgI C'CryG_G
Cuts at: 0 435 510
Size: 435 75
BtsI GCAGTG_nn'
Cuts at: 0 47 356 510
Size: 47 309 154
Fragments arranged by size:
309 154 47
EaeI y'GGCC_r
Cuts at: 0 8 60 510
Size: 8 52 450
Fragments arranged by size:
450 52 8
EagI C'GGCC_G
Cuts at: 0 60 510
Size: 60 450
EarI CTCTTCn'nnn_
Cuts at: 0 414 510
Size: 414 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 91 510
Size: 91 419
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 470 510
Size: 57 413 40
Fragments arranged by size:
413 57 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 277 510
Size: 277 233
EcoO109I rG'GnC_Cy
Cuts at: 0 466 510
Size: 466 44
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 301 510
Size: 301 209
MspA1I CmG'CkG
Cuts at: 0 326 510
Size: 326 184
NcoI C'CATG_G
Cuts at: 0 435 510
Size: 435 75
NheI G'CTAG_C
Cuts at: 0 345 510
Size: 345 165
PasI CC'CwG_GG
Cuts at: 0 257 510
Size: 257 253
PflMI CCAn_nnn'nTGG
Cuts at: 0 462 510
Size: 462 48
PspXI vC'TCGA_Gb
Cuts at: 0 91 510
Size: 91 419
SapI GCTCTTCn'nnn_
Cuts at: 0 414 510
Size: 414 96
SexAI A'CCwGG_T
Cuts at: 0 305 510
Size: 305 205
SmlI C'TyrA_G
Cuts at: 0 91 510
Size: 91 419
StyI C'CwwG_G
Cuts at: 0 176 243 435 510
Size: 176 67 192 75
Fragments arranged by size:
192 176 75 67
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 39 71 510
Size: 39 32 439
Fragments arranged by size:
439 39 32
XhoI C'TCGA_G
Cuts at: 0 91 510
Size: 91 419
XmnI GAAnn'nnTTC
Cuts at: 0 209 510
Size: 209 301
ZraI GAC'GTC
Cuts at: 0 294 510
Size: 294 216
Enzymes that do cut:
AatII AccI AcuI AlfI AvaI BanII BbsI BglI
BmrI BmtI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BsrFI BstYI BstZ17I BtgI BtsI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I FalI MspA1I NcoI
NheI PasI PflMI PspXI SapI SexAI SmlI StyI
TstI XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsgI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI
EcoRV FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
NaeI NarI NdeI NgoMIV NotI NruI NsiI NspI
PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI Tth111I XbaI XcmI XmaI