Restriction map for
pF1KE2336
(Linear) (Six-base) MAPSORT of: KIEE2336.seq Check: 8729 from: 1 to: 3975
KIEE2336.orf
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AccI GT'mk_AC
Cuts at: 0 3872 3975
Size: 3872 103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2096 2097 3023 3193 3975
Size: 2096 1 926 170 782
Fragments arranged by size:
2096 926 782 170 1
AflIII A'CryG_T
Cuts at: 0 60 3975
Size: 60 3915
AleI CACnn'nnGTG
Cuts at: 0 1015 3975
Size: 1015 2960
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 683 3975
Size: 683 3292
AlwNI CAG_nnn'CTG
Cuts at: 0 676 1809 2775 3975
Size: 676 1133 966 1200
Fragments arranged by size:
1200 1133 966 676
ApoI r'AATT_y
Cuts at: 0 287 957 1739 2297 2459 3290 3664 3975
Size: 287 670 782 558 162 831 374 311
Fragments arranged by size:
831 782 670 558 374 311 287 162
AseI AT'TA_AT
Cuts at: 0 350 3975
Size: 350 3625
AvaI C'yCGr_G
Cuts at: 0 1375 1455 3975
Size: 1375 80 2520
Fragments arranged by size:
2520 1375 80
BamHI G'GATC_C
Cuts at: 0 3405 3520 3621 3975
Size: 3405 115 101 354
Fragments arranged by size:
3405 354 115 101
BanII G_rGCy'C
Cuts at: 0 2732 3975
Size: 2732 1243
BbsI GAAGACnn'nnnn_
Cuts at: 0 155 568 1961 2130 3756 3975
Size: 155 413 1393 169 1626 219
Fragments arranged by size:
1626 1393 413 219 169 155
BbvCI CC'TCA_GC
Cuts at: 0 1110 3975
Size: 1110 2865
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 972 1006 3975
Size: 972 34 2969
Fragments arranged by size:
2969 972 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3492 3975
Size: 3492 483
BclI T'GATC_A
Cuts at: 0 615 1034 3975
Size: 615 419 2941
Fragments arranged by size:
2941 615 419
BglII A'GATC_T
Cuts at: 0 2277 2344 3305 3480 3975
Size: 2277 67 961 175 495
Fragments arranged by size:
2277 961 495 175 67
BmrI ACTGGGnnnn_n'
Cuts at: 0 2041 2220 3835 3975
Size: 2041 179 1615 140
Fragments arranged by size:
2041 1615 179 140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1907 2041 2707 3975
Size: 1907 134 666 1268
Fragments arranged by size:
1907 1268 666 134
Bpu10I CC'TnA_GC
Cuts at: 0 1110 2171 3975
Size: 1110 1061 1804
Fragments arranged by size:
1804 1110 1061
BsaI GGTCTCn'nnnn_
Cuts at: 0 1264 1985 2565 3975
Size: 1264 721 580 1410
Fragments arranged by size:
1410 1264 721 580
BsaAI yAC'GTr
Cuts at: 0 1017 3975
Size: 1017 2958
BsaBI GATnn'nnATC
Cuts at: 0 1568 2444 3975
Size: 1568 876 1531
Fragments arranged by size:
1568 1531 876
BsaWI w'CCGG_w
Cuts at: 0 2424 3229 3975
Size: 2424 805 746
Fragments arranged by size:
2424 805 746
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2246 2276 3975
Size: 2246 30 1699
Fragments arranged by size:
2246 1699 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 798 1012 1482 2077 2267 3975
Size: 187 611 214 470 595 190 1708
Fragments arranged by size:
1708 611 595 470 214 190 187
BseYI C'CCAG_C
Cuts at: 0 484 715 3975
Size: 484 231 3260
Fragments arranged by size:
3260 484 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1255 1709 1855 3660 3975
Size: 1255 454 146 1805 315
Fragments arranged by size:
1805 1255 454 315 146
BsiHKAI G_wGCw'C
Cuts at: 0 1022 1376 1536 2019 2127 3422 3975
Size: 1022 354 160 483 108 1295 553
Fragments arranged by size:
1295 1022 553 483 354 160 108
BsiWI C'GTAC_G
Cuts at: 0 401 3975
Size: 401 3574
BsmI GAATG_Cn'
Cuts at: 0 3447 3975
Size: 3447 528
Bsp1286I G_dGCh'C
Cuts at: 0 1022 1376 1536 2019 2127 2732 3422 3975
Size: 1022 354 160 483 108 605 690 553
Fragments arranged by size:
1022 690 605 553 483 354 160 108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2922 3975
Size: 2922 1053
BsrBI CCG'CTC
Cuts at: 0 155 3975
Size: 155 3820
BsrDI GCAATG_nn'
Cuts at: 0 2536 3689 3975
Size: 2536 1153 286
Fragments arranged by size:
2536 1153 286
BsrGI T'GTAC_A
Cuts at: 0 3146 3975
Size: 3146 829
BstAPI GCAn_nnn'nTGC
Cuts at: 0 676 1696 3975
Size: 676 1020 2279
Fragments arranged by size:
2279 1020 676
BstBI TT'CG_AA
Cuts at: 0 1721 3975
Size: 1721 2254
BstEII G'GTnAC_C
Cuts at: 0 1092 3975
Size: 1092 2883
BstXI CCAn_nnnn'nTGG
Cuts at: 0 336 3101 3975
Size: 336 2765 874
Fragments arranged by size:
2765 874 336
BstYI r'GATC_y
Cuts at: 0 2277 2344 3305 3405 3480 3520 3621 3975
Size: 2277 67 961 100 75 40 101 354
Fragments arranged by size:
2277 961 354 101 100 75 67 40
BstZ17I GTA'TAC
Cuts at: 0 3873 3975
Size: 3873 102
Bsu36I CC'TnA_GG
Cuts at: 0 946 3362 3975
Size: 946 2416 613
Fragments arranged by size:
2416 946 613
BtgI C'CryG_G
Cuts at: 0 374 413 554 1337 3975
Size: 374 39 141 783 2638
Fragments arranged by size:
2638 783 374 141 39
BtsI GCAGTG_nn'
Cuts at: 0 655 677 1035 1067 1649 2162 3672 3975
Size: 655 22 358 32 582 513 1510 303
Fragments arranged by size:
1510 655 582 513 358 303 32 22
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2179 2214 3975
Size: 2179 35 1761
Fragments arranged by size:
2179 1761 35
DraI TTT'AAA
Cuts at: 0 93 1892 2465 3975
Size: 93 1799 573 1510
Fragments arranged by size:
1799 1510 573 93
EaeI y'GGCC_r
Cuts at: 0 99 551 2573 3975
Size: 99 452 2022 1402
Fragments arranged by size:
2022 1402 452 99
EarI CTCTTCn'nnn_
Cuts at: 0 1105 2382 2753 3975
Size: 1105 1277 371 1222
Fragments arranged by size:
1277 1222 1105 371
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1907 2041 2096 2097 2707 3023 3193 3975
Size: 1907 134 55 1 610 316 170 782
Fragments arranged by size:
1907 782 610 316 170 134 55 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 169 945 3975
Size: 169 776 3030
Fragments arranged by size:
3030 776 169
EcoO109I rG'GnC_Cy
Cuts at: 0 2897 3975
Size: 2897 1078
EcoRV GAT'ATC
Cuts at: 0 1184 3975
Size: 1184 2791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2127 3975
Size: 2127 1848
HaeII r_GCGC'y
Cuts at: 0 2673 3975
Size: 2673 1302
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 570 602 1111 1143 1168 1200 1249 1263
Size: 570 32 509 32 25 32 49 14
Cuts at: 1263 1281 1295 1572 1604 1867 1899 3107 3139
Size: 18 14 277 32 263 32 1208 32
Cuts at: 3139 3975
Size: 836
Fragments arranged by size:
1208 836 570 509 277 263 49 32
32 32 32 32 32 25 18 14
14
HincII GTy'rAC
Cuts at: 0 2373 3975
Size: 2373 1602
HpaI GTT'AAC
Cuts at: 0 2373 3975
Size: 2373 1602
MluI A'CGCG_T
Cuts at: 0 60 3975
Size: 60 3915
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1538 3516 3975
Size: 1538 1978 459
Fragments arranged by size:
1978 1538 459
MscI TGG'CCA
Cuts at: 0 101 553 3975
Size: 101 452 3422
Fragments arranged by size:
3422 452 101
MslI CAynn'nnrTG
Cuts at: 0 553 1015 2495 2965 2971 3425 3975
Size: 553 462 1480 470 6 454 550
Fragments arranged by size:
1480 553 550 470 462 454 6
MspA1I CmG'CkG
Cuts at: 0 36 1203 1554 2511 2837 3844 3975
Size: 36 1167 351 957 326 1007 131
Fragments arranged by size:
1167 1007 957 351 326 131 36
NcoI C'CATG_G
Cuts at: 0 374 554 3975
Size: 374 180 3421
Fragments arranged by size:
3421 374 180
NdeI CA'TA_TG
Cuts at: 0 515 3975
Size: 515 3460
NspI r_CATG'y
Cuts at: 0 1234 3975
Size: 1234 2741
PasI CC'CwG_GG
Cuts at: 0 1445 3975
Size: 1445 2530
PflMI CCAn_nnn'nTGG
Cuts at: 0 3898 3975
Size: 3898 77
PfoI T'CCnGG_A
Cuts at: 0 1670 1929 3656 3975
Size: 1670 259 1727 319
Fragments arranged by size:
1727 1670 319 259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 40 72 2797 2829 3975
Size: 40 32 2725 32 1146
Fragments arranged by size:
2725 1146 40 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 2897 3975
Size: 2897 1078
PsiI TTA'TAA
Cuts at: 0 838 3975
Size: 838 3137
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 385 417 2309 2341 3130 3162 3975
Size: 385 32 1892 32 789 32 813
Fragments arranged by size:
1892 813 789 385 32 32 32
PstI C_TGCA'G
Cuts at: 0 41 662 2169 3975
Size: 41 621 1507 1806
Fragments arranged by size:
1806 1507 621 41
PvuII CAG'CTG
Cuts at: 0 1203 1554 3975
Size: 1203 351 2421
Fragments arranged by size:
2421 1203 351
SanDI GG'GwC_CC
Cuts at: 0 2897 3975
Size: 2897 1078
SfcI C'TryA_G
Cuts at: 0 37 658 2165 2315 3975
Size: 37 621 1507 150 1660
Fragments arranged by size:
1660 1507 621 150 37
SspI AAT'ATT
Cuts at: 0 1476 3975
Size: 1476 2499
StuI AGG'CCT
Cuts at: 0 1289 2098 3975
Size: 1289 809 1877
Fragments arranged by size:
1877 1289 809
StyI C'CwwG_G
Cuts at: 0 165 374 554 2584 2600 2610 3070 3975
Size: 165 209 180 2030 16 10 460 905
Fragments arranged by size:
2030 905 460 209 180 165 16 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1437 3975
Size: 1437 2538
TatI w'GTAC_w
Cuts at: 0 3146 3975
Size: 3146 829
Tth111I GACn'n_nGTC
Cuts at: 0 2829 3975
Size: 2829 1146
XbaI T'CTAG_A
Cuts at: 0 1993 3975
Size: 1993 1982
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 381 3098 3975
Size: 381 2717 877
Fragments arranged by size:
2717 877 381
XmnI GAAnn'nnTTC
Cuts at: 0 769 2110 2301 3243 3482 3975
Size: 769 1341 191 942 239 493
Fragments arranged by size:
1341 942 769 493 239 191
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApoI AseI
AvaI BamHI BanII BbsI BbvCI BcgI BciVI BclI
BglII BmrI BpmI Bpu10I BsaI BsaAI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmI Bsp1286I
BspMI BsrBI BsrDI BsrGI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtsI CspCI DraI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I
HincII HpaI MluI MmeI MscI MslI MspA1I NcoI
NdeI NspI PasI PflMI PfoI PpiI PpuMI PsiI
PsrI PstI PvuII SanDI SfcI SspI StuI StyI
TaqII TatI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BanI
BbeI BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI
BpuEI BsaHI BsiEI BsmBI BspEI BspHI BsrFI BssHII
BssSI BtgZI ClaI DraIII DrdI EagI EciI EcoICRI
EcoRI FseI FspI FspAI HindIII KasI KpnI MfeI
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PciI PmeI PmlI PshAI PspOMI PspXI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SwaI TstI XhoI XmaI ZraI