Restriction map for
pF1KE2333
(Linear) (Six-base) MAPSORT of: KIEE2333.seq Check: 9906 from: 1 to: 1425
KIEE2333.orf
With 182 enzymes: SgfI *
April 25, 2011 13:19 ..
AatII G_ACGT'C
Cuts at: 0 1048 1425
Size: 1048 377
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1155 1425
Size: 1155 270
AflIII A'CryG_T
Cuts at: 0 1244 1425
Size: 1244 181
AleI CACnn'nnGTG
Cuts at: 0 416 1425
Size: 416 1009
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1280 1425
Size: 1280 145
AlwNI CAG_nnn'CTG
Cuts at: 0 379 1425
Size: 379 1046
ApaI G_GGCC'C
Cuts at: 0 17 1425
Size: 17 1408
ApaLI G'TGCA_C
Cuts at: 0 684 1425
Size: 684 741
ApoI r'AATT_y
Cuts at: 0 1083 1425
Size: 1083 342
AvaI C'yCGr_G
Cuts at: 0 979 1154 1385 1425
Size: 979 175 231 40
Fragments arranged by size:
979 231 175 40
BamHI G'GATC_C
Cuts at: 0 916 1425
Size: 916 509
BanI G'GyrC_C
Cuts at: 0 402 1425
Size: 402 1023
BanII G_rGCy'C
Cuts at: 0 17 573 1161 1425
Size: 17 556 588 264
Fragments arranged by size:
588 556 264 17
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 276 310 1425
Size: 276 34 1115
Fragments arranged by size:
1115 276 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 517 1319 1425
Size: 517 802 106
Fragments arranged by size:
802 517 106
BglI GCCn_nnn'nGGC
Cuts at: 0 181 473 1425
Size: 181 292 952
Fragments arranged by size:
952 292 181
Bme1580I G_kGCm'C
Cuts at: 0 17 332 688 1425
Size: 17 315 356 737
Fragments arranged by size:
737 356 315 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 101 335 1122 1425
Size: 101 234 787 303
Fragments arranged by size:
787 303 234 101
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1180 1425
Size: 1180 245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 455 515 705 1425
Size: 263 192 60 190 720
Fragments arranged by size:
720 263 192 190 60
Bpu10I CC'TnA_GC
Cuts at: 0 139 708 1425
Size: 139 569 717
Fragments arranged by size:
717 569 139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 1425
Size: 443 982
BsaHI Gr'CG_yC
Cuts at: 0 134 1045 1425
Size: 134 911 380
Fragments arranged by size:
911 380 134
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 921 924 1153 1425
Size: 921 3 229 272
Fragments arranged by size:
921 272 229 3
BseYI C'CCAG_C
Cuts at: 0 638 1098 1147 1425
Size: 638 460 49 278
Fragments arranged by size:
638 460 278 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1286 1425
Size: 1286 139
BsiHKAI G_wGCw'C
Cuts at: 0 573 688 1425
Size: 573 115 737
Fragments arranged by size:
737 573 115
BsmBI CGTCTCn'nnnn_
Cuts at: 0 125 1036 1052 1425
Size: 125 911 16 373
Fragments arranged by size:
911 373 125 16
Bsp1286I G_dGCh'C
Cuts at: 0 17 332 573 688 1161 1425
Size: 17 315 241 115 473 264
Fragments arranged by size:
473 315 264 241 115 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 243 1425
Size: 243 1182
BsrBI CCG'CTC
Cuts at: 0 77 956 1016 1425
Size: 77 879 60 409
Fragments arranged by size:
879 409 77 60
BsrDI GCAATG_nn'
Cuts at: 0 597 783 1425
Size: 597 186 642
Fragments arranged by size:
642 597 186
BsrFI r'CCGG_y
Cuts at: 0 925 1425
Size: 925 500
BssSI C'ACGA_G
Cuts at: 0 331 1425
Size: 331 1094
BstAPI GCAn_nnn'nTGC
Cuts at: 0 310 402 1425
Size: 310 92 1023
Fragments arranged by size:
1023 310 92
BstYI r'GATC_y
Cuts at: 0 916 1425
Size: 916 509
Bsu36I CC'TnA_GG
Cuts at: 0 563 1425
Size: 563 862
BtgI C'CryG_G
Cuts at: 0 384 441 511 519 945 1008 1425
Size: 384 57 70 8 426 63 417
Fragments arranged by size:
426 417 384 70 63 57 8
BtsI GCAGTG_nn'
Cuts at: 0 57 768 774 810 822 834 846 858
Size: 57 711 6 36 12 12 12 12
Cuts at: 858 870 906 1252 1313 1414 1425
Size: 12 36 346 61 101 11
Fragments arranged by size:
711 346 101 61 57 36 36 12
12 12 12 12 11 6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 858 893 930 965 1425
Size: 858 35 37 35 460
Fragments arranged by size:
858 460 37 35 35
DraI TTT'AAA
Cuts at: 0 1089 1425
Size: 1089 336
EaeI y'GGCC_r
Cuts at: 0 213 408 1425
Size: 213 195 1017
Fragments arranged by size:
1017 213 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 974 1425
Size: 974 451
EcoICRI GAG'CTC
Cuts at: 0 571 1425
Size: 571 854
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 455 515 705 1155 1425
Size: 263 192 60 190 450 270
Fragments arranged by size:
450 270 263 192 190 60
EcoO109I rG'GnC_Cy
Cuts at: 0 13 14 111 207 1137 1425
Size: 13 1 97 96 930 288
Fragments arranged by size:
930 288 97 96 13 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 484 1425
Size: 484 941
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 606 638 1234 1266 1425
Size: 606 32 596 32 159
Fragments arranged by size:
606 596 159 32 32
MluI A'CGCG_T
Cuts at: 0 1244 1425
Size: 1244 181
MscI TGG'CCA
Cuts at: 0 215 410 1425
Size: 215 195 1015
Fragments arranged by size:
1015 215 195
MslI CAynn'nnrTG
Cuts at: 0 416 1425
Size: 416 1009
MspA1I CmG'CkG
Cuts at: 0 187 386 743 896 1010 1425
Size: 187 199 357 153 114 415
Fragments arranged by size:
415 357 199 187 153 114
NcoI C'CATG_G
Cuts at: 0 441 1425
Size: 441 984
NspI r_CATG'y
Cuts at: 0 1306 1425
Size: 1306 119
PasI CC'CwG_GG
Cuts at: 0 575 1425
Size: 575 850
PflMI CCAn_nnn'nTGG
Cuts at: 0 265 315 610 703 1425
Size: 265 50 295 93 722
Fragments arranged by size:
722 295 265 93 50
PfoI T'CCnGG_A
Cuts at: 0 492 1425
Size: 492 933
PpuMI rG'GwC_Cy
Cuts at: 0 1137 1425
Size: 1137 288
PspOMI G'GGCC_C
Cuts at: 0 13 1425
Size: 13 1412
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1276 1308 1425
Size: 1276 32 117
Fragments arranged by size:
1276 117 32
PstI C_TGCA'G
Cuts at: 0 74 1259 1409 1425
Size: 74 1185 150 16
Fragments arranged by size:
1185 150 74 16
PvuII CAG'CTG
Cuts at: 0 187 1425
Size: 187 1238
SacI G_AGCT'C
Cuts at: 0 573 1425
Size: 573 852
SacII CC_GC'GG
Cuts at: 0 387 1011 1425
Size: 387 624 414
Fragments arranged by size:
624 414 387
ScaI AGT'ACT
Cuts at: 0 1333 1425
Size: 1333 92
SexAI A'CCwGG_T
Cuts at: 0 1133 1425
Size: 1133 292
SfcI C'TryA_G
Cuts at: 0 70 1255 1405 1425
Size: 70 1185 150 20
Fragments arranged by size:
1185 150 70 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 473 1425
Size: 473 952
SmaI CCC'GGG
Cuts at: 0 981 1387 1425
Size: 981 406 38
Fragments arranged by size:
981 406 38
SmlI C'TyrA_G
Cuts at: 0 458 1425
Size: 458 967
SphI G_CATG'C
Cuts at: 0 1306 1425
Size: 1306 119
SspI AAT'ATT
Cuts at: 0 102 1425
Size: 102 1323
StyI C'CwwG_G
Cuts at: 0 114 210 216 441 468 1425
Size: 114 96 6 225 27 957
Fragments arranged by size:
957 225 114 96 27 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1401 1425
Size: 1401 24
TatI w'GTAC_w
Cuts at: 0 1331 1425
Size: 1331 94
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 448 490 646 1425
Size: 448 42 156 779
Fragments arranged by size:
779 448 156 42
XmaI C'CCGG_G
Cuts at: 0 979 1385 1425
Size: 979 406 40
Fragments arranged by size:
979 406 40
ZraI GAC'GTC
Cuts at: 0 1046 1425
Size: 1046 379
Enzymes that do cut:
AatII AcuI AflIII AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI BamHI BanI BanII BcgI BciVI BglI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaHI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstYI Bsu36I BtgI BtsI CspCI
DraI EaeI EciI EcoICRI Eco57MI EcoO109I FalI Hin4I
MluI MscI MslI MspA1I NcoI NspI PasI PflMI
PfoI PpuMI PspOMI PsrI PstI PvuII SacI SacII
ScaI SexAI SfcI SfiI SmaI SmlI SphI SspI
StyI TaqII TatI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI AscI AseI AsiSI AvrII BaeI BbeI BbsI
BbvCI BclI BfrBI BglII BlpI BmgBI BmtI BsaI
BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BspEI
BspHI BsrGI BssHII BstBI BstEII BstXI BstZ17I BtgZI
ClaI DraIII DrdI EagI EarI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PvuI RsrII SalI SanDI SapI
SbfI SfoI SgfI SgrAI SnaBI SpeI SrfI StuI
SwaI TstI Tth111I XbaI XhoI XmnI