Restriction map for
pF1KE2321
(Linear) (Six-base) MAPSORT of: KIEE2321.seq Check: 7435 from: 1 to: 4302
KIEE2321.orf
With 182 enzymes: SgfI *
January 27, 2011 13:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 690 3728 4302
Size: 690 3038 574
Fragments arranged by size:
3038 690 574
AatII G_ACGT'C
Cuts at: 0 1058 1879 4070 4302
Size: 1058 821 2191 232
Fragments arranged by size:
2191 1058 821 232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 1070 1570 1711 1749 2770 3912 4051
Size: 320 750 500 141 38 1021 1142 139
Cuts at: 4051 4302
Size: 251
Fragments arranged by size:
1142 1021 750 500 320 251 141 139
38
AflII C'TTAA_G
Cuts at: 0 967 4302
Size: 967 3335
AflIII A'CryG_T
Cuts at: 0 11 3560 4302
Size: 11 3549 742
Fragments arranged by size:
3549 742 11
AhdI GACnn_n'nnGTC
Cuts at: 0 738 4302
Size: 738 3564
AleI CACnn'nnGTG
Cuts at: 0 2080 2932 3113 3451 4302
Size: 2080 852 181 338 851
Fragments arranged by size:
2080 852 851 338 181
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2336 3365 3424 4302
Size: 2336 1029 59 878
Fragments arranged by size:
2336 1029 878 59
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 30 4302
Size: 30 4272
AlwNI CAG_nnn'CTG
Cuts at: 0 375 438 1757 1772 1902 2224 2801 3087
Size: 375 63 1319 15 130 322 577 286
Cuts at: 3087 3248 3387 4302
Size: 161 139 915
Fragments arranged by size:
1319 915 577 375 322 286 161 139
130 63 15
ApaI G_GGCC'C
Cuts at: 0 298 2600 4302
Size: 298 2302 1702
Fragments arranged by size:
2302 1702 298
ApaLI G'TGCA_C
Cuts at: 0 2395 3577 4302
Size: 2395 1182 725
Fragments arranged by size:
2395 1182 725
ApoI r'AATT_y
Cuts at: 0 2473 4302
Size: 2473 1829
AvaI C'yCGr_G
Cuts at: 0 456 463 3611 3953 4302
Size: 456 7 3148 342 349
Fragments arranged by size:
3148 456 349 342 7
BamHI G'GATC_C
Cuts at: 0 1268 2744 4302
Size: 1268 1476 1558
Fragments arranged by size:
1558 1476 1268
BanI G'GyrC_C
Cuts at: 0 148 678 1655 2167 2501 3763 3860 4302
Size: 148 530 977 512 334 1262 97 442
Fragments arranged by size:
1262 977 530 512 442 334 148 97
BanII G_rGCy'C
Cuts at: 0 298 1015 1217 1613 2600 2774 3031 3263
Size: 298 717 202 396 987 174 257 232
Cuts at: 3263 4029 4302
Size: 766 273
Fragments arranged by size:
987 766 717 396 298 273 257 232
202 174
BbeI G_GCGC'C
Cuts at: 0 152 4302
Size: 152 4150
BbsI GAAGACnn'nnnn_
Cuts at: 0 774 1058 1879 2808 2829 3111 3846 3918
Size: 774 284 821 929 21 282 735 72
Cuts at: 3918 4302
Size: 384
Fragments arranged by size:
929 821 774 735 384 284 282 72
21
BbvCI CC'TCA_GC
Cuts at: 0 476 3397 4302
Size: 476 2921 905
Fragments arranged by size:
2921 905 476
BciVI GTATCCnnnnn_n'
Cuts at: 0 3659 4302
Size: 3659 643
BclI T'GATC_A
Cuts at: 0 122 1101 4302
Size: 122 979 3201
Fragments arranged by size:
3201 979 122
BfrBI ATG'CAT
Cuts at: 0 1023 1529 4302
Size: 1023 506 2773
Fragments arranged by size:
2773 1023 506
BglI GCCn_nnn'nGGC
Cuts at: 0 590 2024 4302
Size: 590 1434 2278
Fragments arranged by size:
2278 1434 590
BglII A'GATC_T
Cuts at: 0 2339 4240 4302
Size: 2339 1901 62
Fragments arranged by size:
2339 1901 62
BlpI GC'TnA_GC
Cuts at: 0 637 4302
Size: 637 3665
Bme1580I G_kGCm'C
Cuts at: 0 298 681 1786 2057 2399 2600 3581 4302
Size: 298 383 1105 271 342 201 981 721
Fragments arranged by size:
1105 981 721 383 342 298 271 201
BmrI ACTGGGnnnn_n'
Cuts at: 0 939 2049 2740 4302
Size: 939 1110 691 1562
Fragments arranged by size:
1562 1110 939 691
BmtI G_CTAG'C
Cuts at: 0 2218 4302
Size: 2218 2084
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 525 601 4302
Size: 525 76 3701
Fragments arranged by size:
3701 525 76
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 340 1610 1765 1855 2930 3007 3187
Size: 262 78 1270 155 90 1075 77 180
Cuts at: 3187 3232 3484 4302
Size: 45 252 818
Fragments arranged by size:
1270 1075 818 262 252 180 155 90
78 77 45
Bpu10I CC'TnA_GC
Cuts at: 0 223 476 1607 3007 3397 4302
Size: 223 253 1131 1400 390 905
Fragments arranged by size:
1400 1131 905 390 253 223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 901 1186 4302
Size: 601 300 285 3116
Fragments arranged by size:
3116 601 300 285
BsaI GGTCTCn'nnnn_
Cuts at: 0 262 316 3892 4302
Size: 262 54 3576 410
Fragments arranged by size:
3576 410 262 54
BsaAI yAC'GTr
Cuts at: 0 276 3981 4302
Size: 276 3705 321
Fragments arranged by size:
3705 321 276
BsaBI GATnn'nnATC
Cuts at: 0 1915 4302
Size: 1915 2387
BsaHI Gr'CG_yC
Cuts at: 0 149 1055 1876 4067 4142 4302
Size: 149 906 821 2191 75 160
Fragments arranged by size:
2191 906 821 160 149 75
BsaWI w'CCGG_w
Cuts at: 0 2065 3662 4302
Size: 2065 1597 640
Fragments arranged by size:
2065 1597 640
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1954 1984 1993 2023 2542 2557 2572 2587
Size: 1954 30 9 30 519 15 15 15
Cuts at: 2587 2840 2870 3076 3106 4003 4033 4302
Size: 253 30 206 30 897 30 269
Fragments arranged by size:
1954 897 519 269 253 206 30 30
30 30 30 15 15 15 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2550 3229 3481 3540 3639 3716 4302
Size: 2550 679 252 59 99 77 586
Fragments arranged by size:
2550 679 586 252 99 77 59
BseYI C'CCAG_C
Cuts at: 0 1445 1500 3509 3709 4115 4302
Size: 1445 55 2009 200 406 187
Fragments arranged by size:
2009 1445 406 200 187 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1293 1531 2536 3261 4003 4302
Size: 1293 238 1005 725 742 299
Fragments arranged by size:
1293 1005 742 725 299 238
BsiEI CG_ry'CG
Cuts at: 0 207 422 777 4302
Size: 207 215 355 3525
Fragments arranged by size:
3525 355 215 207
BsiHKAI G_wGCw'C
Cuts at: 0 976 1217 1613 2399 2774 3263 3581 3708
Size: 976 241 396 786 375 489 318 127
Cuts at: 3708 4302
Size: 594
Fragments arranged by size:
976 786 594 489 396 375 318 241
127
BsiWI C'GTAC_G
Cuts at: 0 4217 4302
Size: 4217 85
BsmI GAATG_Cn'
Cuts at: 0 1251 3153 4302
Size: 1251 1902 1149
Fragments arranged by size:
1902 1251 1149
BsmBI CGTCTCn'nnnn_
Cuts at: 0 61 2562 3067 4302
Size: 61 2501 505 1235
Fragments arranged by size:
2501 1235 505 61
Bsp1286I G_dGCh'C
Cuts at: 0 298 681 976 1015 1217 1613 1786 2057
Size: 298 383 295 39 202 396 173 271
Cuts at: 2057 2399 2600 2774 3031 3263 3581 3708 4029
Size: 342 201 174 257 232 318 127 321
Cuts at: 4029 4302
Size: 273
Fragments arranged by size:
396 383 342 321 318 298 295 273
271 257 232 202 201 174 173 127
39
BspEI T'CCGG_A
Cuts at: 0 2065 4302
Size: 2065 2237
BspHI T'CATG_A
Cuts at: 0 4109 4302
Size: 4109 193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 690 1278 3728 4302
Size: 690 588 2450 574
Fragments arranged by size:
2450 690 588 574
BsrBI CCG'CTC
Cuts at: 0 102 1578 3162 3219 4302
Size: 102 1476 1584 57 1083
Fragments arranged by size:
1584 1476 1083 102 57
BsrDI GCAATG_nn'
Cuts at: 0 257 813 1605 2451 2898 3770 4250 4302
Size: 257 556 792 846 447 872 480 52
Fragments arranged by size:
872 846 792 556 480 447 257 52
BsrFI r'CCGG_y
Cuts at: 0 206 417 421 2108 3489 3766 3863 4144
Size: 206 211 4 1687 1381 277 97 281
Cuts at: 4144 4302
Size: 158
Fragments arranged by size:
1687 1381 281 277 211 206 158 97
4
BsrGI T'GTAC_A
Cuts at: 0 3938 4302
Size: 3938 364
BssSI C'ACGA_G
Cuts at: 0 2672 3133 3142 4302
Size: 2672 461 9 1160
Fragments arranged by size:
2672 1160 461 9
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3387 4302
Size: 3387 915
BstBI TT'CG_AA
Cuts at: 0 2126 4302
Size: 2126 2176
BstEII G'GTnAC_C
Cuts at: 0 2427 4302
Size: 2427 1875
BstXI CCAn_nnnn'nTGG
Cuts at: 0 318 396 531 2247 3453 3849 4302
Size: 318 78 135 1716 1206 396 453
Fragments arranged by size:
1716 1206 453 396 318 135 78
BstYI r'GATC_y
Cuts at: 0 399 1268 2339 2451 2744 4240 4302
Size: 399 869 1071 112 293 1496 62
Fragments arranged by size:
1496 1071 869 399 293 112 62
Bsu36I CC'TnA_GG
Cuts at: 0 861 1378 4302
Size: 861 517 2924
Fragments arranged by size:
2924 861 517
BtgI C'CryG_G
Cuts at: 0 395 1313 1320 2375 3636 3842 4073 4302
Size: 395 918 7 1055 1261 206 231 229
Fragments arranged by size:
1261 1055 918 395 231 229 206 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2606 4302
Size: 2606 1696
BtsI GCAGTG_nn'
Cuts at: 0 449 4302
Size: 449 3853
DraIII CAC_nnn'GTG
Cuts at: 0 2709 4302
Size: 2709 1593
DrdI GACnn_nn'nnGTC
Cuts at: 0 1432 3513 3682 4302
Size: 1432 2081 169 620
Fragments arranged by size:
2081 1432 620 169
EaeI y'GGCC_r
Cuts at: 0 204 419 1844 2237 2261 2636 2936 3491
Size: 204 215 1425 393 24 375 300 555
Cuts at: 3491 3590 4302
Size: 99 712
Fragments arranged by size:
1425 712 555 393 375 300 215 204
99 24
EagI C'GGCC_G
Cuts at: 0 204 419 4302
Size: 204 215 3883
Fragments arranged by size:
3883 215 204
EarI CTCTTCn'nnn_
Cuts at: 0 64 1081 1154 1618 2524 2780 2902 3890
Size: 64 1017 73 464 906 256 122 988
Cuts at: 3890 4259 4302
Size: 369 43
Fragments arranged by size:
1017 988 906 464 369 256 122 73
64 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1257 1962 2911 3253 4024 4302
Size: 1257 705 949 342 771 278
Fragments arranged by size:
1257 949 771 705 342 278
EcoICRI GAG'CTC
Cuts at: 0 1215 1611 2772 3261 4302
Size: 1215 396 1161 489 1041
Fragments arranged by size:
1215 1161 1041 489 396
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 320 340 1070 1570 1610 1711 1749
Size: 262 58 20 730 500 40 101 38
Cuts at: 1749 1765 1855 2770 2930 3007 3187 3232 3484
Size: 16 90 915 160 77 180 45 252
Cuts at: 3484 3912 4051 4302
Size: 428 139 251
Fragments arranged by size:
915 730 500 428 262 252 251 180
160 139 101 90 77 58 45 40
38 20 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 172 1585 1888 3400 4302
Size: 172 1413 303 1512 902
Fragments arranged by size:
1512 1413 902 303 172
EcoO109I rG'GnC_Cy
Cuts at: 0 78 294 295 344 379 452 460 542
Size: 78 216 1 49 35 73 8 82
Cuts at: 542 555 1497 1680 2177 2798 3138 4302
Size: 13 942 183 497 621 340 1164
Fragments arranged by size:
1164 942 621 497 340 216 183 82
78 73 49 35 13 8 1
EcoRV GAT'ATC
Cuts at: 0 3813 4302
Size: 3813 489
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1443 1875 2832 3942 4302
Size: 1443 432 957 1110 360
Fragments arranged by size:
1443 1110 957 432 360
FseI GG_CCGG'CC
Cuts at: 0 210 4302
Size: 210 4092
HaeII r_GCGC'y
Cuts at: 0 152 239 1757 1903 4302
Size: 152 87 1518 146 2399
Fragments arranged by size:
2399 1518 152 146 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 439 471 724 756 1954 1986 2840 2872
Size: 439 32 253 32 1198 32 854 32
Cuts at: 2872 3460 3492 4302
Size: 588 32 810
Fragments arranged by size:
1198 854 810 588 439 253 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 201 1962 2859 3047 3186 4302
Size: 201 1761 897 188 139 1116
Fragments arranged by size:
1761 1116 897 201 188 139
HindIII A'AGCT_T
Cuts at: 0 2315 3786 4302
Size: 2315 1471 516
Fragments arranged by size:
2315 1471 516
KasI G'GCGC_C
Cuts at: 0 148 4302
Size: 148 4154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1619 1742 3735 3840 4302
Size: 1619 123 1993 105 462
Fragments arranged by size:
1993 1619 462 123 105
MscI TGG'CCA
Cuts at: 0 1846 2263 2638 3592 4302
Size: 1846 417 375 954 710
Fragments arranged by size:
1846 954 710 417 375
MslI CAynn'nnrTG
Cuts at: 0 361 1006 2080 2932 3113 3451 4302
Size: 361 645 1074 852 181 338 851
Fragments arranged by size:
1074 852 851 645 361 338 181
MspA1I CmG'CkG
Cuts at: 0 2015 2903 2995 3339 3513 3801 4005 4302
Size: 2015 888 92 344 174 288 204 297
Fragments arranged by size:
2015 888 344 297 288 204 174 92
NaeI GCC'GGC
Cuts at: 0 208 423 3491 3865 4146 4302
Size: 208 215 3068 374 281 156
Fragments arranged by size:
3068 374 281 215 208 156
NarI GG'CG_CC
Cuts at: 0 149 4302
Size: 149 4153
NcoI C'CATG_G
Cuts at: 0 2375 3842 4073 4302
Size: 2375 1467 231 229
Fragments arranged by size:
2375 1467 231 229
NgoMIV G'CCGG_C
Cuts at: 0 206 421 3489 3863 4144 4302
Size: 206 215 3068 374 281 158
Fragments arranged by size:
3068 374 281 215 206 158
NheI G'CTAG_C
Cuts at: 0 2214 4302
Size: 2214 2088
NsiI A_TGCA'T
Cuts at: 0 1025 1531 4302
Size: 1025 506 2771
Fragments arranged by size:
2771 1025 506
NspI r_CATG'y
Cuts at: 0 15 484 1039 1725 2158 3564 4302
Size: 15 469 555 686 433 1406 738
Fragments arranged by size:
1406 738 686 555 469 433 15
PasI CC'CwG_GG
Cuts at: 0 375 1491 4302
Size: 375 1116 2811
Fragments arranged by size:
2811 1116 375
PciI A'CATG_T
Cuts at: 0 11 3560 4302
Size: 11 3549 742
Fragments arranged by size:
3549 742 11
PflMI CCAn_nnn'nTGG
Cuts at: 0 1902 4302
Size: 1902 2400
PfoI T'CCnGG_A
Cuts at: 0 1832 2720 4302
Size: 1832 888 1582
Fragments arranged by size:
1832 1582 888
PmlI CAC'GTG
Cuts at: 0 276 4302
Size: 276 4026
PpuMI rG'GwC_Cy
Cuts at: 0 344 379 460 555 1497 1680 2177 2798
Size: 344 35 81 95 942 183 497 621
Cuts at: 2798 3138 4302
Size: 340 1164
Fragments arranged by size:
1164 942 621 497 344 340 183 95
81 35
PspOMI G'GGCC_C
Cuts at: 0 294 2596 4302
Size: 294 2302 1706
Fragments arranged by size:
2302 1706 294
PstI C_TGCA'G
Cuts at: 0 1289 3092 3338 3344 3527 3908 3998 4302
Size: 1289 1803 246 6 183 381 90 304
Fragments arranged by size:
1803 1289 381 304 246 183 90 6
PvuII CAG'CTG
Cuts at: 0 2903 2995 3339 3513 4005 4302
Size: 2903 92 344 174 492 297
Fragments arranged by size:
2903 492 344 297 174 92
SacI G_AGCT'C
Cuts at: 0 1217 1613 2774 3263 4302
Size: 1217 396 1161 489 1039
Fragments arranged by size:
1217 1161 1039 489 396
SanDI GG'GwC_CC
Cuts at: 0 344 379 460 1497 2177 4302
Size: 344 35 81 1037 680 2125
Fragments arranged by size:
2125 1037 680 344 81 35
SapI GCTCTTCn'nnn_
Cuts at: 0 1081 1618 2524 4302
Size: 1081 537 906 1778
Fragments arranged by size:
1778 1081 906 537
SbfI CC_TGCA'GG
Cuts at: 0 3908 3998 4302
Size: 3908 90 304
Fragments arranged by size:
3908 304 90
ScaI AGT'ACT
Cuts at: 0 3301 4302
Size: 3301 1001
SfcI C'TryA_G
Cuts at: 0 1285 1929 3088 3334 3340 3523 3888 3904
Size: 1285 644 1159 246 6 183 365 16
Cuts at: 3904 3994 4302
Size: 90 308
Fragments arranged by size:
1285 1159 644 365 308 246 183 90
16 6
SfoI GGC'GCC
Cuts at: 0 150 4302
Size: 150 4152
SgrAI Cr'CCGG_yG
Cuts at: 0 4144 4302
Size: 4144 158
SmaI CCC'GGG
Cuts at: 0 465 3955 4302
Size: 465 3490 347
Fragments arranged by size:
3490 465 347
SmlI C'TyrA_G
Cuts at: 0 616 916 967 1201 3611 4302
Size: 616 300 51 234 2410 691
Fragments arranged by size:
2410 691 616 300 234 51
SphI G_CATG'C
Cuts at: 0 484 1039 4302
Size: 484 555 3263
Fragments arranged by size:
3263 555 484
StuI AGG'CCT
Cuts at: 0 1047 3292 4302
Size: 1047 2245 1010
Fragments arranged by size:
2245 1047 1010
StyI C'CwwG_G
Cuts at: 0 212 588 686 2363 2375 3842 4073 4302
Size: 212 376 98 1677 12 1467 231 229
Fragments arranged by size:
1677 1467 376 231 229 212 98 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 791 2031 3032 3103 3723 4302
Size: 791 1240 1001 71 620 579
Fragments arranged by size:
1240 1001 791 620 579 71
TatI w'GTAC_w
Cuts at: 0 998 3299 3938 4302
Size: 998 2301 639 364
Fragments arranged by size:
2301 998 639 364
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 77 109 701 733 2693 2725 3698 3730
Size: 77 32 592 32 1960 32 973 32
Cuts at: 3730 4302
Size: 572
Fragments arranged by size:
1960 973 592 572 77 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 778 4302
Size: 778 3524
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 393 1143 4302
Size: 393 750 3159
Fragments arranged by size:
3159 750 393
XhoI C'TCGA_G
Cuts at: 0 3611 4302
Size: 3611 691
XmaI C'CCGG_G
Cuts at: 0 463 3953 4302
Size: 463 3490 349
Fragments arranged by size:
3490 463 349
XmnI GAAnn'nnTTC
Cuts at: 0 2815 4302
Size: 2815 1487
ZraI GAC'GTC
Cuts at: 0 1056 1877 4068 4302
Size: 1056 821 2191 234
Fragments arranged by size:
2191 1056 821 234
Enzymes that do cut:
AarI AatII AcuI AflII AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI FseI HaeII Hin4I
HincII HindIII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NsiI NspI PasI PciI
PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI
SanDI SapI SbfI ScaI SfcI SfoI SgrAI SmaI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AgeI AscI AseI AsiSI
AvrII BaeI BcgI BmgBI BssHII BstZ17I ClaI CspCI
DraI EcoRI FspI FspAI HpaI KpnI MfeI MluI
NdeI NotI NruI PacI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SexAI SfiI
SgfI SnaBI SpeI SrfI SspI SwaI XbaI