Restriction map for
pF1KE2317
(Linear) (Six-base) MAPSORT of: KIEE2317.seq Check: 2445 from: 1 to: 3153
KIEE2317.orf
With 182 enzymes: SgfI *
January 12, 2011 16:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 876 894 3153
Size: 876 18 2259
Fragments arranged by size:
2259 876 18
AatII G_ACGT'C
Cuts at: 0 1943 3153
Size: 1943 1210
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2179 3153
Size: 2179 974
AfeI AGC'GCT
Cuts at: 0 199 1993 3153
Size: 199 1794 1160
Fragments arranged by size:
1794 1160 199
AflIII A'CryG_T
Cuts at: 0 362 3153
Size: 362 2791
AleI CACnn'nnGTG
Cuts at: 0 436 802 2029 2776 2796 3153
Size: 436 366 1227 747 20 357
Fragments arranged by size:
1227 747 436 366 357 20
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2503 3153
Size: 2503 650
AlwNI CAG_nnn'CTG
Cuts at: 0 441 891 1656 1806 1914 3153
Size: 441 450 765 150 108 1239
Fragments arranged by size:
1239 765 450 441 150 108
ApaI G_GGCC'C
Cuts at: 0 7 397 1386 2407 2653 3153
Size: 7 390 989 1021 246 500
Fragments arranged by size:
1021 989 500 390 246 7
ApoI r'AATT_y
Cuts at: 0 2392 3153
Size: 2392 761
AvaI C'yCGr_G
Cuts at: 0 141 224 1636 2071 3153
Size: 141 83 1412 435 1082
Fragments arranged by size:
1412 1082 435 141 83
AvrII C'CTAG_G
Cuts at: 0 2445 3153
Size: 2445 708
BamHI G'GATC_C
Cuts at: 0 2631 3153
Size: 2631 522
BanI G'GyrC_C
Cuts at: 0 217 598 637 646 811 821 910 979
Size: 217 381 39 9 165 10 89 69
Cuts at: 979 1018 1419 2034 3153
Size: 39 401 615 1119
Fragments arranged by size:
1119 615 401 381 217 165 89 69
39 39 10 9
BanII G_rGCy'C
Cuts at: 0 7 233 397 901 1173 1226 1386 2407
Size: 7 226 164 504 272 53 160 1021
Cuts at: 2407 2653 3066 3153
Size: 246 413 87
Fragments arranged by size:
1021 504 413 272 246 226 164 160
87 53 7
BbeI G_GCGC'C
Cuts at: 0 641 914 983 3153
Size: 641 273 69 2170
Fragments arranged by size:
2170 641 273 69
BbsI GAAGACnn'nnnn_
Cuts at: 0 464 1023 1605 3153
Size: 464 559 582 1548
Fragments arranged by size:
1548 582 559 464
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 135 169 2796 2830 3153
Size: 135 34 2627 34 323
Fragments arranged by size:
2627 323 135 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 99 2504 3153
Size: 99 2405 649
Fragments arranged by size:
2405 649 99
BclI T'GATC_A
Cuts at: 0 3023 3153
Size: 3023 130
BglI GCCn_nnn'nGGC
Cuts at: 0 155 401 596 1094 1581 2014 3153
Size: 155 246 195 498 487 433 1139
Fragments arranged by size:
1139 498 487 433 246 195 155
BlpI GC'TnA_GC
Cuts at: 0 850 1915 2290 3153
Size: 850 1065 375 863
Fragments arranged by size:
1065 863 850 375
Bme1580I G_kGCm'C
Cuts at: 0 7 222 242 283 397 423 601 816
Size: 7 215 20 41 114 26 178 215
Cuts at: 816 1386 1424 2407 2653 2727 2749 3153
Size: 570 38 983 246 74 22 404
Fragments arranged by size:
983 570 404 246 215 215 178 114
74 41 38 26 22 20 7
BmgBI CAC'GTC
Cuts at: 0 2219 3153
Size: 2219 934
BmrI ACTGGGnnnn_n'
Cuts at: 0 1085 3153
Size: 1085 2068
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 531 3153
Size: 531 2622
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 501 595 913 1573 1678 1828 1909 2263
Size: 501 94 318 660 105 150 81 354
Cuts at: 2263 2473 2567 3153
Size: 210 94 586
Fragments arranged by size:
660 586 501 354 318 210 150 105
94 94 81
Bpu10I CC'TnA_GC
Cuts at: 0 1957 3153
Size: 1957 1196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2368 3153
Size: 2368 785
BsaI GGTCTCn'nnnn_
Cuts at: 0 863 2086 2543 3153
Size: 863 1223 457 610
Fragments arranged by size:
1223 863 610 457
BsaAI yAC'GTr
Cuts at: 0 489 2915 3153
Size: 489 2426 238
Fragments arranged by size:
2426 489 238
BsaBI GATnn'nnATC
Cuts at: 0 319 3153
Size: 319 2834
BsaHI Gr'CG_yC
Cuts at: 0 638 911 980 1775 1940 2006 3153
Size: 638 273 69 795 165 66 1147
Fragments arranged by size:
1147 795 638 273 165 69 66
BsaWI w'CCGG_w
Cuts at: 0 257 1943 3153
Size: 257 1686 1210
Fragments arranged by size:
1686 1210 257
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 365 395 3153
Size: 365 30 2758
Fragments arranged by size:
2758 365 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 894 1216 2965 3153
Size: 894 322 1749 188
Fragments arranged by size:
1749 894 322 188
BseYI C'CCAG_C
Cuts at: 0 13 594 1238 1263 1755 2109 2855 3015
Size: 13 581 644 25 492 354 746 160
Cuts at: 3015 3153
Size: 138
Fragments arranged by size:
746 644 581 492 354 160 138 25
13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 1430 2164 2221 3020 3153
Size: 766 664 734 57 799 133
Fragments arranged by size:
799 766 734 664 133 57
BsiEI CG_ry'CG
Cuts at: 0 74 167 1576 1746 2963 3153
Size: 74 93 1409 170 1217 190
Fragments arranged by size:
1409 1217 190 170 93 74
BsiHKAI G_wGCw'C
Cuts at: 0 233 1173 3153
Size: 233 940 1980
Fragments arranged by size:
1980 940 233
BsmBI CGTCTCn'nnnn_
Cuts at: 0 93 571 1931 3153
Size: 93 478 1360 1222
Fragments arranged by size:
1360 1222 478 93
Bsp1286I G_dGCh'C
Cuts at: 0 7 222 233 242 283 397 423 601
Size: 7 215 11 9 41 114 26 178
Cuts at: 601 816 901 1173 1226 1386 1424 2407 2653
Size: 215 85 272 53 160 38 983 246
Cuts at: 2653 2727 2749 3066 3153
Size: 74 22 317 87
Fragments arranged by size:
983 317 272 246 215 215 178 160
114 87 85 74 53 41 38 26
22 11 9 7
BspEI T'CCGG_A
Cuts at: 0 1943 3153
Size: 1943 1210
BspHI T'CATG_A
Cuts at: 0 1034 3153
Size: 1034 2119
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 212 753 876 881 894 1233 3153
Size: 212 541 123 5 13 339 1920
Fragments arranged by size:
1920 541 339 212 123 13 5
BsrBI CCG'CTC
Cuts at: 0 189 312 3153
Size: 189 123 2841
Fragments arranged by size:
2841 189 123
BsrDI GCAATG_nn'
Cuts at: 0 504 929 2481 2526 3153
Size: 504 425 1552 45 627
Fragments arranged by size:
1552 627 504 425 45
BsrFI r'CCGG_y
Cuts at: 0 73 1571 1594 2306 2958 3153
Size: 73 1498 23 712 652 195
Fragments arranged by size:
1498 712 652 195 73 23
BsrGI T'GTAC_A
Cuts at: 0 1196 3153
Size: 1196 1957
BssHII G'CGCG_C
Cuts at: 0 20 3153
Size: 20 3133
BssSI C'ACGA_G
Cuts at: 0 2011 2792 3153
Size: 2011 781 361
Fragments arranged by size:
2011 781 361
BstEII G'GTnAC_C
Cuts at: 0 2172 3153
Size: 2172 981
BstXI CCAn_nnnn'nTGG
Cuts at: 0 936 2521 2898 3153
Size: 936 1585 377 255
Fragments arranged by size:
1585 936 377 255
BstYI r'GATC_y
Cuts at: 0 2631 3153
Size: 2631 522
BtgI C'CryG_G
Cuts at: 0 817 1251 1617 2498 2514 2993 3153
Size: 817 434 366 881 16 479 160
Fragments arranged by size:
881 817 479 434 366 160 16
BtsI GCAGTG_nn'
Cuts at: 0 287 1605 3153
Size: 287 1318 1548
Fragments arranged by size:
1548 1318 287
DraIII CAC_nnn'GTG
Cuts at: 0 1371 2733 3153
Size: 1371 1362 420
Fragments arranged by size:
1371 1362 420
EaeI y'GGCC_r
Cuts at: 0 71 342 383 1118 1280 1457 1573 2960
Size: 71 271 41 735 162 177 116 1387
Cuts at: 2960 2990 3153
Size: 30 163
Fragments arranged by size:
1387 735 271 177 163 162 116 71
41 30
EagI C'GGCC_G
Cuts at: 0 71 1573 2960 3153
Size: 71 1502 1387 193
Fragments arranged by size:
1502 1387 193 71
EarI CTCTTCn'nnn_
Cuts at: 0 157 3106 3153
Size: 157 2949 47
Fragments arranged by size:
2949 157 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 81 881 1684 3118 3153
Size: 81 800 803 1434 35
Fragments arranged by size:
1434 803 800 81 35
EcoICRI GAG'CTC
Cuts at: 0 231 1171 3153
Size: 231 940 1982
Fragments arranged by size:
1982 940 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 501 595 913 1573 1678 1828 1909 2179
Size: 501 94 318 660 105 150 81 270
Cuts at: 2179 2263 2473 2567 3153
Size: 84 210 94 586
Fragments arranged by size:
660 586 501 318 270 210 150 105
94 94 84 81
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1792 3153
Size: 1792 1361
EcoO109I rG'GnC_Cy
Cuts at: 0 4 393 394 720 966 2075 2190 2435
Size: 4 389 1 326 246 1109 115 245
Cuts at: 2435 2525 2649 2650 3153
Size: 90 124 1 503
Fragments arranged by size:
1109 503 389 326 246 245 124 115
90 4 1 1
EcoRI G'AATT_C
Cuts at: 0 2392 3153
Size: 2392 761
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3066 3153
Size: 3066 87
HaeII r_GCGC'y
Cuts at: 0 201 641 914 929 983 1995 2020 3153
Size: 201 440 273 15 54 1012 25 1133
Fragments arranged by size:
1133 1012 440 273 201 54 25 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 398 430 1728 1760 2241 2273 2540 2572
Size: 398 32 1298 32 481 32 267 32
Cuts at: 2572 2584 2616 2920 2952 3153
Size: 12 32 304 32 201
Fragments arranged by size:
1298 481 398 304 267 201 32 32
32 32 32 32 12
HindIII A'AGCT_T
Cuts at: 0 2316 3153
Size: 2316 837
KasI G'GCGC_C
Cuts at: 0 637 910 979 3153
Size: 637 273 69 2174
Fragments arranged by size:
2174 637 273 69
MscI TGG'CCA
Cuts at: 0 344 385 1120 1282 3153
Size: 344 41 735 162 1871
Fragments arranged by size:
1871 735 344 162 41
MslI CAynn'nnrTG
Cuts at: 0 299 325 436 802 1711 1966 2029 2170
Size: 299 26 111 366 909 255 63 141
Cuts at: 2170 2519 2776 2796 2896 3153
Size: 349 257 20 100 257
Fragments arranged by size:
909 366 349 299 257 257 255 141
111 100 63 26 20
MspA1I CmG'CkG
Cuts at: 0 465 594 605 1432 1609 2205 2628 3153
Size: 465 129 11 827 177 596 423 525
Fragments arranged by size:
827 596 525 465 423 177 129 11
NaeI GCC'GGC
Cuts at: 0 75 1573 1596 2960 3153
Size: 75 1498 23 1364 193
Fragments arranged by size:
1498 1364 193 75 23
NarI GG'CG_CC
Cuts at: 0 638 911 980 3153
Size: 638 273 69 2173
Fragments arranged by size:
2173 638 273 69
NcoI C'CATG_G
Cuts at: 0 1251 2514 3153
Size: 1251 1263 639
Fragments arranged by size:
1263 1251 639
NdeI CA'TA_TG
Cuts at: 0 830 3153
Size: 830 2323
NgoMIV G'CCGG_C
Cuts at: 0 73 1571 1594 2958 3153
Size: 73 1498 23 1364 195
Fragments arranged by size:
1498 1364 195 73 23
NspI r_CATG'y
Cuts at: 0 366 588 3153
Size: 366 222 2565
Fragments arranged by size:
2565 366 222
PasI CC'CwG_GG
Cuts at: 0 2587 3153
Size: 2587 566
PciI A'CATG_T
Cuts at: 0 362 3153
Size: 362 2791
PflMI CCAn_nnn'nTGG
Cuts at: 0 1656 2139 3153
Size: 1656 483 1014
Fragments arranged by size:
1656 1014 483
PmlI CAC'GTG
Cuts at: 0 2915 3153
Size: 2915 238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1813 1845 3153
Size: 1813 32 1308
Fragments arranged by size:
1813 1308 32
PpuMI rG'GwC_Cy
Cuts at: 0 720 966 2190 2435 2525 3153
Size: 720 246 1224 245 90 628
Fragments arranged by size:
1224 720 628 246 245 90
PshAI GACnn'nnGTC
Cuts at: 0 2440 3153
Size: 2440 713
PspOMI G'GGCC_C
Cuts at: 0 3 393 1382 2403 2649 3153
Size: 3 390 989 1021 246 504
Fragments arranged by size:
1021 989 504 390 246 3
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 613 645 1504 1536 3153
Size: 613 32 859 32 1617
Fragments arranged by size:
1617 859 613 32 32
PstI C_TGCA'G
Cuts at: 0 402 2210 3153
Size: 402 1808 943
Fragments arranged by size:
1808 943 402
PvuI CG_AT'CG
Cuts at: 0 1746 3153
Size: 1746 1407
PvuII CAG'CTG
Cuts at: 0 594 1432 2205 2628 3153
Size: 594 838 773 423 525
Fragments arranged by size:
838 773 594 525 423
SacI G_AGCT'C
Cuts at: 0 233 1173 3153
Size: 233 940 1980
Fragments arranged by size:
1980 940 233
SanDI GG'GwC_CC
Cuts at: 0 2435 3153
Size: 2435 718
SapI GCTCTTCn'nnn_
Cuts at: 0 3106 3153
Size: 3106 47
SbfI CC_TGCA'GG
Cuts at: 0 402 3153
Size: 402 2751
SfcI C'TryA_G
Cuts at: 0 398 1521 2206 3153
Size: 398 1123 685 947
Fragments arranged by size:
1123 947 685 398
SfoI GGC'GCC
Cuts at: 0 639 912 981 3153
Size: 639 273 69 2172
Fragments arranged by size:
2172 639 273 69
SmaI CCC'GGG
Cuts at: 0 143 226 2073 3153
Size: 143 83 1847 1080
Fragments arranged by size:
1847 1080 143 83
SmlI C'TyrA_G
Cuts at: 0 2383 3153
Size: 2383 770
StyI C'CwwG_G
Cuts at: 0 732 1251 1415 2445 2514 3153
Size: 732 519 164 1030 69 639
Fragments arranged by size:
1030 732 639 519 164 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 153 448 631 947 2329 2852 2986 3153
Size: 153 295 183 316 1382 523 134 167
Fragments arranged by size:
1382 523 316 295 183 167 153 134
TatI w'GTAC_w
Cuts at: 0 629 1196 1928 3153
Size: 629 567 732 1225
Fragments arranged by size:
1225 732 629 567
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 365 397 2224 2256 3153
Size: 365 32 1827 32 897
Fragments arranged by size:
1827 897 365 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2839 3153
Size: 2839 314
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2895 3153
Size: 2895 258
XmaI C'CCGG_G
Cuts at: 0 141 224 2071 3153
Size: 141 83 1847 1082
Fragments arranged by size:
1847 1082 141 83
XmnI GAAnn'nnTTC
Cuts at: 0 2119 3153
Size: 2119 1034
ZraI GAC'GTC
Cuts at: 0 1941 3153
Size: 1941 1212
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AleI AlfI AlwNI
ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstEII BstXI BstYI BtgI BtsI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HindIII KasI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PflMI PmlI PpiI PpuMI PshAI PspOMI
PsrI PstI PvuI PvuII SacI SanDI SapI SbfI
SfcI SfoI SmaI SmlI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AloI ApaLI
AscI AseI AsiSI BaeI BbvCI BfrBI BglII BmtI
BsiWI BsmI BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EcoRV FseI FspI FspAI HincII
HpaI KpnI MfeI MluI MmeI NheI NotI NruI
NsiI PacI PfoI PmeI PsiI PspXI RsrII SacII
SalI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI XhoI