(Linear) (Six-base) MAPSORT of: KIEE2304.seq  Check: 6684  from: 1  to: 900



KIEE2304.orf

 With 182 enzymes: SgfI * 

                        November 10, 2010 10:12  ..

AccI GT'mk_AC

Cuts at:      0     416     818     900 
   Size:        416     402      82

  Fragments arranged by size: 

                 416     402      82
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      33     900 
   Size:         33     867
 
AlwNI CAG_nnn'CTG

Cuts at:      0     115     588     900 
   Size:        115     473     312

  Fragments arranged by size: 

                 473     312     115
 
ApoI r'AATT_y

Cuts at:      0     772     787     853     900 
   Size:        772      15      66      47

  Fragments arranged by size: 

                 772      66      47      15
 
AvrII C'CTAG_G

Cuts at:      0      30     900 
   Size:         30     870
 
BglI GCCn_nnn'nGGC

Cuts at:      0      32     900 
   Size:         32     868
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     383     900 
   Size:        383     517
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     559     900 
   Size:        559     341
 
BsmI GAATG_Cn'

Cuts at:      0     726     900 
   Size:        726     174
 
BspHI T'CATG_A

Cuts at:      0     867     900 
   Size:        867      33
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     383     900 
   Size:        383     517
 
BsrDI GCAATG_nn'

Cuts at:      0     726     900 
   Size:        726     174
 
BtgI C'CryG_G

Cuts at:      0     368     615     900 
   Size:        368     247     285

  Fragments arranged by size: 

                 368     285     247
 
BtsI GCAGTG_nn'

Cuts at:      0      17     900 
   Size:         17     883
 
EaeI y'GGCC_r

Cuts at:      0     119     429     900 
   Size:        119     310     471

  Fragments arranged by size: 

                 471     310     119
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     383     900 
   Size:        383     517
 
EcoO109I rG'GnC_Cy

Cuts at:      0     585     900 
   Size:        585     315
 
EcoRI G'AATT_C

Cuts at:      0     772     900 
   Size:        772     128
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     476     508     511     543     729     761     868     900 
   Size:        476      32       3      32     186      32     107      32

  Fragments arranged by size: 

                 476     186     107      32      32      32      32       3
            
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     215     900 
   Size:        215     685
 
MscI TGG'CCA

Cuts at:      0     121     900 
   Size:        121     779
 
MslI CAynn'nnrTG

Cuts at:      0     212     335     731     782     797     900 
   Size:        212     123     396      51      15     103

  Fragments arranged by size: 

                 396     212     123     103      51      15
 
MspA1I CmG'CkG

Cuts at:      0     645     900 
   Size:        645     255
 
NdeI CA'TA_TG

Cuts at:      0     423     900 
   Size:        423     477
 
PpuMI rG'GwC_Cy

Cuts at:      0     585     900 
   Size:        585     315
 
PshAI GACnn'nnGTC

Cuts at:      0     459     900 
   Size:        459     441
 
PvuII CAG'CTG

Cuts at:      0     645     900 
   Size:        645     255
 
SfcI C'TryA_G

Cuts at:      0     509     900 
   Size:        509     391
 
StyI C'CwwG_G

Cuts at:      0      30     900 
   Size:         30     870
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     348     380     667     699     900 
   Size:        348      32     287      32     201

  Fragments arranged by size: 

                 348     287     201      32      32
 

 Enzymes that do cut:

     AccI     AlfI    AlwNI     ApoI    AvrII     BglI     BpmI    BseRI
     BsmI    BspHI    BspMI    BsrDI     BtgI     BtsI     EaeI  Eco57MI
 EcoO109I    EcoRI    Hin4I     MmeI     MscI     MslI   MspA1I     NdeI
    PpuMI    PshAI    PvuII     SfcI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI