Restriction map for
pF1KE2300
(Linear) (Six-base) MAPSORT of: KIEE2300.seq Check: 7082 from: 1 to: 2571
KIEE2300.orf
With 182 enzymes: SgfI *
November 10, 2010 10:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1437 2571
Size: 1437 1134
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1087 1520 2438 2571
Size: 1087 433 918 133
Fragments arranged by size:
1087 918 433 133
AfeI AGC'GCT
Cuts at: 0 290 2571
Size: 290 2281
AleI CACnn'nnGTG
Cuts at: 0 2507 2571
Size: 2507 64
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1563 2571
Size: 1563 1008
ApoI r'AATT_y
Cuts at: 0 119 332 421 555 603 768 975 1512
Size: 119 213 89 134 48 165 207 537
Cuts at: 1512 1568 2263 2375 2457 2571
Size: 56 695 112 82 114
Fragments arranged by size:
695 537 213 207 165 134 119 114
112 89 82 56 48
AseI AT'TA_AT
Cuts at: 0 2213 2571
Size: 2213 358
AvaI C'yCGr_G
Cuts at: 0 144 2571
Size: 144 2427
BanII G_rGCy'C
Cuts at: 0 2354 2571
Size: 2354 217
BbsI GAAGACnn'nnnn_
Cuts at: 0 95 865 1081 2087 2571
Size: 95 770 216 1006 484
Fragments arranged by size:
1006 770 484 216 95
BfrBI ATG'CAT
Cuts at: 0 1550 2571
Size: 1550 1021
BglII A'GATC_T
Cuts at: 0 2286 2571
Size: 2286 285
BmrI ACTGGGnnnn_n'
Cuts at: 0 817 2571
Size: 817 1754
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1388 2085 2571
Size: 1388 697 486
Fragments arranged by size:
1388 697 486
Bpu10I CC'TnA_GC
Cuts at: 0 2348 2571
Size: 2348 223
BsaAI yAC'GTr
Cuts at: 0 385 2571
Size: 385 2186
BsaBI GATnn'nnATC
Cuts at: 0 2249 2571
Size: 2249 322
BsaWI w'CCGG_w
Cuts at: 0 646 764 1227 1703 2571
Size: 646 118 463 476 868
Fragments arranged by size:
868 646 476 463 118
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 604 2571
Size: 604 1967
BsiHKAI G_wGCw'C
Cuts at: 0 2354 2571
Size: 2354 217
BsmI GAATG_Cn'
Cuts at: 0 919 1552 1979 2571
Size: 919 633 427 592
Fragments arranged by size:
919 633 592 427
Bsp1286I G_dGCh'C
Cuts at: 0 2354 2571
Size: 2354 217
BspEI T'CCGG_A
Cuts at: 0 1227 2571
Size: 1227 1344
BspHI T'CATG_A
Cuts at: 0 2253 2571
Size: 2253 318
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1437 1470 2571
Size: 1437 33 1101
Fragments arranged by size:
1437 1101 33
BstEII G'GTnAC_C
Cuts at: 0 760 2571
Size: 760 1811
BstXI CCAn_nnnn'nTGG
Cuts at: 0 191 1921 2449 2571
Size: 191 1730 528 122
Fragments arranged by size:
1730 528 191 122
BstYI r'GATC_y
Cuts at: 0 937 2286 2571
Size: 937 1349 285
Fragments arranged by size:
1349 937 285
Bsu36I CC'TnA_GG
Cuts at: 0 2157 2571
Size: 2157 414
BtgI C'CryG_G
Cuts at: 0 1861 2571
Size: 1861 710
DraI TTT'AAA
Cuts at: 0 81 251 560 980 1583 2315 2571
Size: 81 170 309 420 603 732 256
Fragments arranged by size:
732 603 420 309 256 170 81
EaeI y'GGCC_r
Cuts at: 0 309 401 1455 2402 2571
Size: 309 92 1054 947 169
Fragments arranged by size:
1054 947 309 169 92
EarI CTCTTCn'nnn_
Cuts at: 0 810 2571
Size: 810 1761
EcoICRI GAG'CTC
Cuts at: 0 2352 2571
Size: 2352 219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1087 1388 1520 2085 2438 2571
Size: 1087 301 132 565 353 133
Fragments arranged by size:
1087 565 353 301 133 132
EcoO109I rG'GnC_Cy
Cuts at: 0 2322 2571
Size: 2322 249
EcoRI G'AATT_C
Cuts at: 0 332 768 2457 2571
Size: 332 436 1689 114
Fragments arranged by size:
1689 436 332 114
EcoRV GAT'ATC
Cuts at: 0 2339 2571
Size: 2339 232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1437 2571
Size: 1437 1134
HaeII r_GCGC'y
Cuts at: 0 292 2571
Size: 292 2279
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1327 1359 2137 2169 2199 2231 2571
Size: 1327 32 778 32 30 32 340
Fragments arranged by size:
1327 778 340 32 32 32 30
HincII GTy'rAC
Cuts at: 0 156 828 1445 2394 2571
Size: 156 672 617 949 177
Fragments arranged by size:
949 672 617 177 156
HindIII A'AGCT_T
Cuts at: 0 108 2571
Size: 108 2463
HpaI GTT'AAC
Cuts at: 0 156 828 2394 2571
Size: 156 672 1566 177
Fragments arranged by size:
1566 672 177 156
MfeI C'AATT_G
Cuts at: 0 35 281 2571
Size: 35 246 2290
Fragments arranged by size:
2290 246 35
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1782 2571
Size: 1782 789
MscI TGG'CCA
Cuts at: 0 311 403 1457 2404 2571
Size: 311 92 1054 947 167
Fragments arranged by size:
1054 947 311 167 92
MslI CAynn'nnrTG
Cuts at: 0 1326 1795 1801 1919 2366 2507 2571
Size: 1326 469 6 118 447 141 64
Fragments arranged by size:
1326 469 447 141 118 64 6
MspA1I CmG'CkG
Cuts at: 0 579 2571
Size: 579 1992
NcoI C'CATG_G
Cuts at: 0 1861 2571
Size: 1861 710
NsiI A_TGCA'T
Cuts at: 0 1552 2571
Size: 1552 1019
NspI r_CATG'y
Cuts at: 0 738 2236 2571
Size: 738 1498 335
Fragments arranged by size:
1498 738 335
PmlI CAC'GTG
Cuts at: 0 385 2571
Size: 385 2186
PpuMI rG'GwC_Cy
Cuts at: 0 2322 2571
Size: 2322 249
PvuII CAG'CTG
Cuts at: 0 579 2571
Size: 579 1992
SacI G_AGCT'C
Cuts at: 0 2354 2571
Size: 2354 217
SanDI GG'GwC_CC
Cuts at: 0 2322 2571
Size: 2322 249
SfcI C'TryA_G
Cuts at: 0 134 506 2505 2571
Size: 134 372 1999 66
Fragments arranged by size:
1999 372 134 66
SspI AAT'ATT
Cuts at: 0 1818 2571
Size: 1818 753
StyI C'CwwG_G
Cuts at: 0 1373 1861 2405 2571
Size: 1373 488 544 166
Fragments arranged by size:
1373 544 488 166
TatI w'GTAC_w
Cuts at: 0 1787 2171 2571
Size: 1787 384 400
Fragments arranged by size:
1787 400 384
XbaI T'CTAG_A
Cuts at: 0 904 2571
Size: 904 1667
XmnI GAAnn'nnTTC
Cuts at: 0 290 957 1572 2571
Size: 290 667 615 999
Fragments arranged by size:
999 667 615 290
Enzymes that do cut:
AarI AcuI AfeI AleI AlfI ApoI AseI AvaI
BanII BbsI BfrBI BglII BmrI BpmI Bpu10I BsaAI
BsaBI BsaWI BseRI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BstEII BstXI BstYI Bsu36I BtgI DraI EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI
MspA1I NcoI NsiI NspI PmlI PpuMI PvuII SacI
SanDI SfcI SspI StyI TatI XbaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BclI BglI
BlpI Bme1580I BmgBI BmtI BplI BpuEI BsaI BsaHI
BsaXI BseYI BsgI BsiEI BsiWI BsmBI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI
BtsI ClaI CspCI DraIII DrdI EagI EciI EcoNI
FseI FspI FspAI KasI KpnI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI
Tth111I XcmI XhoI XmaI ZraI