Restriction map for
pF1KE2202
(Linear) (Six-base) MAPSORT of: KIEE2202.seq Check: 8742 from: 1 to: 2829
KIEE2202.orf
With 182 enzymes: SgfI *
September 21, 2016 11:20 ..
AccI GT'mk_AC
Cuts at: 0 54 2445 2573 2829
Size: 54 2391 128 256
Fragments arranged by size:
2391 256 128 54
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1310 1550 1616 1811 2018 2741 2829
Size: 1310 240 66 195 207 723 88
Fragments arranged by size:
1310 723 240 207 195 88 66
AfeI AGC'GCT
Cuts at: 0 1495 2829
Size: 1495 1334
AflIII A'CryG_T
Cuts at: 0 1984 2227 2829
Size: 1984 243 602
Fragments arranged by size:
1984 602 243
ApoI r'AATT_y
Cuts at: 0 159 356 451 561 758 904 1270 1357
Size: 159 197 95 110 197 146 366 87
Cuts at: 1357 1368 1565 1823 2829
Size: 11 197 258 1006
Fragments arranged by size:
1006 366 258 197 197 197 159 146
110 95 87 11
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 488 521 2829
Size: 488 33 2308
Fragments arranged by size:
2308 488 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 1750 2829
Size: 1750 1079
BciVI GTATCCnnnnn_n'
Cuts at: 0 1137 2580 2829
Size: 1137 1443 249
Fragments arranged by size:
1443 1137 249
BclI T'GATC_A
Cuts at: 0 426 2829
Size: 426 2403
BfrBI ATG'CAT
Cuts at: 0 1656 2829
Size: 1656 1173
BglII A'GATC_T
Cuts at: 0 943 1186 1903 2829
Size: 943 243 717 926
Fragments arranged by size:
943 926 717 243
Bme1580I G_kGCm'C
Cuts at: 0 69 534 2829
Size: 69 465 2295
Fragments arranged by size:
2295 465 69
BmrI ACTGGGnnnn_n'
Cuts at: 0 1099 2615 2744 2829
Size: 1099 1516 129 85
Fragments arranged by size:
1516 1099 129 85
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1820 2039 2303 2829
Size: 1820 219 264 526
Fragments arranged by size:
1820 526 264 219
BsaI GGTCTCn'nnnn_
Cuts at: 0 980 2829
Size: 980 1849
BsaAI yAC'GTr
Cuts at: 0 70 2829
Size: 70 2759
BsaBI GATnn'nnATC
Cuts at: 0 2537 2829
Size: 2537 292
BsaHI Gr'CG_yC
Cuts at: 0 1575 2829
Size: 1575 1254
BsaWI w'CCGG_w
Cuts at: 0 10 2786 2829
Size: 10 2776 43
Fragments arranged by size:
2776 43 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1793 1823 2282 2312 2829
Size: 1793 30 459 30 517
Fragments arranged by size:
1793 517 459 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1621 2270 2829
Size: 1621 649 559
Fragments arranged by size:
1621 649 559
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 998 1928 2829
Size: 998 930 901
Fragments arranged by size:
998 930 901
BsiHKAI G_wGCw'C
Cuts at: 0 1012 2829
Size: 1012 1817
BsmI GAATG_Cn'
Cuts at: 0 1221 2829
Size: 1221 1608
Bsp1286I G_dGCh'C
Cuts at: 0 69 534 1012 2829
Size: 69 465 478 1817
Fragments arranged by size:
1817 478 465 69
BspEI T'CCGG_A
Cuts at: 0 2786 2829
Size: 2786 43
BspHI T'CATG_A
Cuts at: 0 1815 2829
Size: 1815 1014
BstAPI GCAn_nnn'nTGC
Cuts at: 0 230 2829
Size: 230 2599
BstEII G'GTnAC_C
Cuts at: 0 2689 2829
Size: 2689 140
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1586 2829
Size: 1586 1243
BstYI r'GATC_y
Cuts at: 0 943 1186 1903 2393 2457 2514 2829
Size: 943 243 717 490 64 57 315
Fragments arranged by size:
943 717 490 315 243 64 57
BstZ17I GTA'TAC
Cuts at: 0 2446 2574 2829
Size: 2446 128 255
Fragments arranged by size:
2446 255 128
BtsI GCAGTG_nn'
Cuts at: 0 1010 2829
Size: 1010 1819
DraI TTT'AAA
Cuts at: 0 1784 2829
Size: 1784 1045
EaeI y'GGCC_r
Cuts at: 0 2655 2829
Size: 2655 174
EarI CTCTTCn'nnn_
Cuts at: 0 674 1382 1391 2829
Size: 674 708 9 1438
Fragments arranged by size:
1438 708 674 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1310 1550 1616 1811 1820 2018 2039 2303
Size: 1310 240 66 195 9 198 21 264
Cuts at: 2303 2741 2829
Size: 438 88
Fragments arranged by size:
1310 438 264 240 198 195 88 66
21 9
EcoO109I rG'GnC_Cy
Cuts at: 0 2484 2829
Size: 2484 345
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1172 1315 1512 2072 2442 2829
Size: 1172 143 197 560 370 387
Fragments arranged by size:
1172 560 387 370 197 143
HaeII r_GCGC'y
Cuts at: 0 1497 2829
Size: 1497 1332
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 676 708 2314 2346 2829
Size: 676 32 1606 32 483
Fragments arranged by size:
1606 676 483 32 32
HincII GTy'rAC
Cuts at: 0 55 2370 2682 2829
Size: 55 2315 312 147
Fragments arranged by size:
2315 312 147 55
HpaI GTT'AAC
Cuts at: 0 2682 2829
Size: 2682 147
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 902 2219 2829
Size: 902 1317 610
Fragments arranged by size:
1317 902 610
MscI TGG'CCA
Cuts at: 0 2657 2829
Size: 2657 172
MslI CAynn'nnrTG
Cuts at: 0 434 2829
Size: 434 2395
MspA1I CmG'CkG
Cuts at: 0 1288 2829
Size: 1288 1541
NsiI A_TGCA'T
Cuts at: 0 1658 2829
Size: 1658 1171
NspI r_CATG'y
Cuts at: 0 847 1988 2653 2829
Size: 847 1141 665 176
Fragments arranged by size:
1141 847 665 176
PciI A'CATG_T
Cuts at: 0 1984 2829
Size: 1984 845
PmlI CAC'GTG
Cuts at: 0 70 2829
Size: 70 2759
PpuMI rG'GwC_Cy
Cuts at: 0 2484 2829
Size: 2484 345
PsiI TTA'TAA
Cuts at: 0 910 2771 2829
Size: 910 1861 58
Fragments arranged by size:
1861 910 58
PvuII CAG'CTG
Cuts at: 0 1288 2829
Size: 1288 1541
SalI G'TCGA_C
Cuts at: 0 53 2829
Size: 53 2776
SapI GCTCTTCn'nnn_
Cuts at: 0 1382 2829
Size: 1382 1447
ScaI AGT'ACT
Cuts at: 0 1139 2248 2312 2733 2829
Size: 1139 1109 64 421 96
Fragments arranged by size:
1139 1109 421 96 64
SfcI C'TryA_G
Cuts at: 0 36 289 2829
Size: 36 253 2540
Fragments arranged by size:
2540 253 36
SpeI A'CTAG_T
Cuts at: 0 2181 2829
Size: 2181 648
SphI G_CATG'C
Cuts at: 0 847 2829
Size: 847 1982
SspI AAT'ATT
Cuts at: 0 1710 2358 2829
Size: 1710 648 471
Fragments arranged by size:
1710 648 471
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1118 1581 2829
Size: 1118 463 1248
Fragments arranged by size:
1248 1118 463
TatI w'GTAC_w
Cuts at: 0 154 586 1137 1770 2246 2310 2701 2731
Size: 154 432 551 633 476 64 391 30
Cuts at: 2731 2829
Size: 98
Fragments arranged by size:
633 551 476 432 391 154 98 64
30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2282 2314 2829
Size: 2282 32 515
Fragments arranged by size:
2282 515 32
XbaI T'CTAG_A
Cuts at: 0 1231 1900 2829
Size: 1231 669 929
Fragments arranged by size:
1231 929 669
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1586 2829
Size: 1586 1243
XmnI GAAnn'nnTTC
Cuts at: 0 1566 2801 2829
Size: 1566 1235 28
Fragments arranged by size:
1566 1235 28
Enzymes that do cut:
AccI AcuI AfeI AflIII ApoI BaeI BbsI BciVI
BclI BfrBI BglII Bme1580I BmrI BpmI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI
Bsp1286I BspEI BspHI BstAPI BstEII BstXI BstYI BstZ17I
BtsI DraI EaeI EarI Eco57MI EcoO109I FalI HaeII
Hin4I HincII HpaI MmeI MscI MslI MspA1I NsiI
NspI PciI PmlI PpuMI PsiI PvuII SalI SapI
ScaI SfcI SpeI SphI SspI TaqII TatI TstI
XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbvCI BcgI
BglI BlpI BmgBI BmtI BplI Bpu10I BpuEI BseYI
BsiEI BsiWI BsmBI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI Bsu36I BtgI BtgZI ClaI CspCI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HindIII KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PflMI PfoI PmeI PpiI PshAI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SrfI StuI StyI SwaI Tth111I XhoI XmaI
ZraI