Restriction map for
pF1KE2200
(Linear) (Six-base) MAPSORT of: KIEE2200.seq Check: 7529 from: 1 to: 1551
KIEE2200.orf
With 182 enzymes: SgfI *
November 26, 2010 10:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1068 1417 1428 1551
Size: 1068 349 11 123
Fragments arranged by size:
1068 349 123 11
AfeI AGC'GCT
Cuts at: 0 147 1551
Size: 147 1404
AflIII A'CryG_T
Cuts at: 0 19 1107 1551
Size: 19 1088 444
Fragments arranged by size:
1088 444 19
AleI CACnn'nnGTG
Cuts at: 0 1247 1551
Size: 1247 304
AlwNI CAG_nnn'CTG
Cuts at: 0 468 1551
Size: 468 1083
ApaI G_GGCC'C
Cuts at: 0 536 1551
Size: 536 1015
AvaI C'yCGr_G
Cuts at: 0 105 330 616 1551
Size: 105 225 286 935
Fragments arranged by size:
935 286 225 105
BamHI G'GATC_C
Cuts at: 0 741 1551
Size: 741 810
BanI G'GyrC_C
Cuts at: 0 68 130 306 786 1551
Size: 68 62 176 480 765
Fragments arranged by size:
765 480 176 68 62
BanII G_rGCy'C
Cuts at: 0 211 536 763 967 1056 1551
Size: 211 325 227 204 89 495
Fragments arranged by size:
495 325 227 211 204 89
BbeI G_GCGC'C
Cuts at: 0 134 310 1551
Size: 134 176 1241
Fragments arranged by size:
1241 176 134
BbsI GAAGACnn'nnnn_
Cuts at: 0 1074 1405 1551
Size: 1074 331 146
Fragments arranged by size:
1074 331 146
BbvCI CC'TCA_GC
Cuts at: 0 853 1551
Size: 853 698
BfrBI ATG'CAT
Cuts at: 0 908 1515 1551
Size: 908 607 36
Fragments arranged by size:
908 607 36
BglI GCCn_nnn'nGGC
Cuts at: 0 139 758 1551
Size: 139 619 793
Fragments arranged by size:
793 619 139
Bme1580I G_kGCm'C
Cuts at: 0 279 536 1551
Size: 279 257 1015
Fragments arranged by size:
1015 279 257
BmgBI CAC'GTC
Cuts at: 0 216 1108 1551
Size: 216 892 443
Fragments arranged by size:
892 443 216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 951 1136 1522 1551
Size: 142 809 185 386 29
Fragments arranged by size:
809 386 185 142 29
Bpu10I CC'TnA_GC
Cuts at: 0 469 853 1551
Size: 469 384 698
Fragments arranged by size:
698 469 384
BsaI GGTCTCn'nnnn_
Cuts at: 0 1144 1551
Size: 1144 407
BsaAI yAC'GTr
Cuts at: 0 20 1551
Size: 20 1531
BsaHI Gr'CG_yC
Cuts at: 0 37 131 181 307 562 673 859 1551
Size: 37 94 50 126 255 111 186 692
Fragments arranged by size:
692 255 186 126 111 94 50 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 22 52 1551
Size: 22 30 1499
Fragments arranged by size:
1499 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 183 472 536 1551
Size: 183 289 64 1015
Fragments arranged by size:
1015 289 183 64
BseYI C'CCAG_C
Cuts at: 0 434 553 607 676 765 896 958 1551
Size: 434 119 54 69 89 131 62 593
Fragments arranged by size:
593 434 131 119 89 69 62 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 813 1551
Size: 813 738
BsiEI CG_ry'CG
Cuts at: 0 1141 1551
Size: 1141 410
BsiHKAI G_wGCw'C
Cuts at: 0 967 1551
Size: 967 584
BsmI GAATG_Cn'
Cuts at: 0 910 1091 1551
Size: 910 181 460
Fragments arranged by size:
910 460 181
BsmBI CGTCTCn'nnnn_
Cuts at: 0 44 160 1551
Size: 44 116 1391
Fragments arranged by size:
1391 116 44
Bsp1286I G_dGCh'C
Cuts at: 0 211 279 536 763 967 1056 1551
Size: 211 68 257 227 204 89 495
Fragments arranged by size:
495 257 227 211 204 89 68
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 611 1551
Size: 611 940
BsrBI CCG'CTC
Cuts at: 0 56 290 1551
Size: 56 234 1261
Fragments arranged by size:
1261 234 56
BsrFI r'CCGG_y
Cuts at: 0 33 309 375 832 1551
Size: 33 276 66 457 719
Fragments arranged by size:
719 457 276 66 33
BssSI C'ACGA_G
Cuts at: 0 454 1551
Size: 454 1097
BstEII G'GTnAC_C
Cuts at: 0 657 1551
Size: 657 894
BstXI CCAn_nnnn'nTGG
Cuts at: 0 505 1551
Size: 505 1046
BstYI r'GATC_y
Cuts at: 0 741 1551
Size: 741 810
BtgI C'CryG_G
Cuts at: 0 174 746 1551
Size: 174 572 805
Fragments arranged by size:
805 572 174
BtsI GCAGTG_nn'
Cuts at: 0 307 469 876 1551
Size: 307 162 407 675
Fragments arranged by size:
675 407 307 162
EaeI y'GGCC_r
Cuts at: 0 495 570 1456 1551
Size: 495 75 886 95
Fragments arranged by size:
886 495 95 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 371 539 1489 1551
Size: 371 168 950 62
Fragments arranged by size:
950 371 168 62
EcoICRI GAG'CTC
Cuts at: 0 965 1551
Size: 965 586
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 951 1068 1136 1417 1428 1522 1551
Size: 142 809 117 68 281 11 94 29
Fragments arranged by size:
809 281 142 117 94 68 29 11
EcoO109I rG'GnC_Cy
Cuts at: 0 6 102 222 533 1551
Size: 6 96 120 311 1018
Fragments arranged by size:
1018 311 120 96 6
HaeII r_GCGC'y
Cuts at: 0 134 149 310 338 476 1549 1551
Size: 134 15 161 28 138 1073 2
Fragments arranged by size:
1073 161 138 134 28 15 2
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 903 935 1551
Size: 22 32 849 32 616
Fragments arranged by size:
849 616 32 32 22
KasI G'GCGC_C
Cuts at: 0 130 306 1551
Size: 130 176 1245
Fragments arranged by size:
1245 176 130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 1412 1551
Size: 236 1176 139
Fragments arranged by size:
1176 236 139
MscI TGG'CCA
Cuts at: 0 1458 1551
Size: 1458 93
MslI CAynn'nnrTG
Cuts at: 0 1247 1263 1337 1353 1551
Size: 1247 16 74 16 198
Fragments arranged by size:
1247 198 74 16 16
MspA1I CmG'CkG
Cuts at: 0 176 611 827 1448 1542 1551
Size: 176 435 216 621 94 9
Fragments arranged by size:
621 435 216 176 94 9
NaeI GCC'GGC
Cuts at: 0 35 1551
Size: 35 1516
NarI GG'CG_CC
Cuts at: 0 131 307 1551
Size: 131 176 1244
Fragments arranged by size:
1244 176 131
NcoI C'CATG_G
Cuts at: 0 746 1551
Size: 746 805
NgoMIV G'CCGG_C
Cuts at: 0 33 1551
Size: 33 1518
NsiI A_TGCA'T
Cuts at: 0 910 1517 1551
Size: 910 607 34
Fragments arranged by size:
910 607 34
NspI r_CATG'y
Cuts at: 0 1515 1551
Size: 1515 36
PasI CC'CwG_GG
Cuts at: 0 255 1551
Size: 255 1296
PfoI T'CCnGG_A
Cuts at: 0 26 98 1551
Size: 26 72 1453
Fragments arranged by size:
1453 72 26
PmlI CAC'GTG
Cuts at: 0 20 1551
Size: 20 1531
PpuMI rG'GwC_Cy
Cuts at: 0 6 102 222 1551
Size: 6 96 120 1329
Fragments arranged by size:
1329 120 96 6
PspOMI G'GGCC_C
Cuts at: 0 532 1551
Size: 532 1019
PvuII CAG'CTG
Cuts at: 0 611 827 1448 1542 1551
Size: 611 216 621 94 9
Fragments arranged by size:
621 611 216 94 9
RsrII CG'GwC_CG
Cuts at: 0 1385 1551
Size: 1385 166
SacI G_AGCT'C
Cuts at: 0 967 1551
Size: 967 584
SacII CC_GC'GG
Cuts at: 0 177 1551
Size: 177 1374
SanDI GG'GwC_CC
Cuts at: 0 102 1551
Size: 102 1449
ScaI AGT'ACT
Cuts at: 0 781 1551
Size: 781 770
SfcI C'TryA_G
Cuts at: 0 73 864 1551
Size: 73 791 687
Fragments arranged by size:
791 687 73
SfoI GGC'GCC
Cuts at: 0 132 308 1551
Size: 132 176 1243
Fragments arranged by size:
1243 176 132
SgrAI Cr'CCGG_yG
Cuts at: 0 309 1551
Size: 309 1242
SmaI CCC'GGG
Cuts at: 0 107 332 1551
Size: 107 225 1219
Fragments arranged by size:
1219 225 107
SmlI C'TyrA_G
Cuts at: 0 616 1551
Size: 616 935
StyI C'CwwG_G
Cuts at: 0 746 1551
Size: 746 805
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 56 247 529 757 1551
Size: 56 191 282 228 794
Fragments arranged by size:
794 282 228 191 56
TatI w'GTAC_w
Cuts at: 0 779 1551
Size: 779 772
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1539 1551
Size: 1539 12
XhoI C'TCGA_G
Cuts at: 0 616 1551
Size: 616 935
XmaI C'CCGG_G
Cuts at: 0 105 330 1551
Size: 105 225 1221
Fragments arranged by size:
1221 225 105
XmnI GAAnn'nnTTC
Cuts at: 0 1293 1348 1551
Size: 1293 55 203
Fragments arranged by size:
1293 203 55
Enzymes that do cut:
AcuI AfeI AflIII AleI AlwNI ApaI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BfrBI BglI Bme1580I
BmgBI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrFI BssSI BstEII BstXI BstYI BtgI BtsI
EaeI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PfoI PmlI PpuMI PspOMI PvuII
RsrII SacI SacII SanDI ScaI SfcI SfoI SgrAI
SmaI SmlI StyI TaqII TatI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BcgI BciVI BclI BglII BlpI BmrI BmtI
BplI BpuEI BsaBI BsaWI BsiWI BspEI BspHI BsrDI
BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EarI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI NdeI NheI NotI NruI PacI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI
PstI PvuI SalI SapI SbfI SexAI SfiI SgfI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI ZraI