(Linear) (Six-base) MAPSORT of: KIEE2200.seq Check: 7529 from: 1 to: 1551 KIEE2200.orf With 182 enzymes: SgfI * November 26, 2010 10:36 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1068 1417 1428 1551 Size: 1068 349 11 123 Fragments arranged by size: 1068 349 123 11 AfeI AGC'GCT Cuts at: 0 147 1551 Size: 147 1404 AflIII A'CryG_T Cuts at: 0 19 1107 1551 Size: 19 1088 444 Fragments arranged by size: 1088 444 19 AleI CACnn'nnGTG Cuts at: 0 1247 1551 Size: 1247 304 AlwNI CAG_nnn'CTG Cuts at: 0 468 1551 Size: 468 1083 ApaI G_GGCC'C Cuts at: 0 536 1551 Size: 536 1015 AvaI C'yCGr_G Cuts at: 0 105 330 616 1551 Size: 105 225 286 935 Fragments arranged by size: 935 286 225 105 BamHI G'GATC_C Cuts at: 0 741 1551 Size: 741 810 BanI G'GyrC_C Cuts at: 0 68 130 306 786 1551 Size: 68 62 176 480 765 Fragments arranged by size: 765 480 176 68 62 BanII G_rGCy'C Cuts at: 0 211 536 763 967 1056 1551 Size: 211 325 227 204 89 495 Fragments arranged by size: 495 325 227 211 204 89 BbeI G_GCGC'C Cuts at: 0 134 310 1551 Size: 134 176 1241 Fragments arranged by size: 1241 176 134 BbsI GAAGACnn'nnnn_ Cuts at: 0 1074 1405 1551 Size: 1074 331 146 Fragments arranged by size: 1074 331 146 BbvCI CC'TCA_GC Cuts at: 0 853 1551 Size: 853 698 BfrBI ATG'CAT Cuts at: 0 908 1515 1551 Size: 908 607 36 Fragments arranged by size: 908 607 36 BglI GCCn_nnn'nGGC Cuts at: 0 139 758 1551 Size: 139 619 793 Fragments arranged by size: 793 619 139 Bme1580I G_kGCm'C Cuts at: 0 279 536 1551 Size: 279 257 1015 Fragments arranged by size: 1015 279 257 BmgBI CAC'GTC Cuts at: 0 216 1108 1551 Size: 216 892 443 Fragments arranged by size: 892 443 216 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 951 1136 1522 1551 Size: 142 809 185 386 29 Fragments arranged by size: 809 386 185 142 29 Bpu10I CC'TnA_GC Cuts at: 0 469 853 1551 Size: 469 384 698 Fragments arranged by size: 698 469 384 BsaI GGTCTCn'nnnn_ Cuts at: 0 1144 1551 Size: 1144 407 BsaAI yAC'GTr Cuts at: 0 20 1551 Size: 20 1531 BsaHI Gr'CG_yC Cuts at: 0 37 131 181 307 562 673 859 1551 Size: 37 94 50 126 255 111 186 692 Fragments arranged by size: 692 255 186 126 111 94 50 37 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 22 52 1551 Size: 22 30 1499 Fragments arranged by size: 1499 30 22 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 183 472 536 1551 Size: 183 289 64 1015 Fragments arranged by size: 1015 289 183 64 BseYI C'CCAG_C Cuts at: 0 434 553 607 676 765 896 958 1551 Size: 434 119 54 69 89 131 62 593 Fragments arranged by size: 593 434 131 119 89 69 62 54 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 813 1551 Size: 813 738 BsiEI CG_ry'CG Cuts at: 0 1141 1551 Size: 1141 410 BsiHKAI G_wGCw'C Cuts at: 0 967 1551 Size: 967 584 BsmI GAATG_Cn' Cuts at: 0 910 1091 1551 Size: 910 181 460 Fragments arranged by size: 910 460 181 BsmBI CGTCTCn'nnnn_ Cuts at: 0 44 160 1551 Size: 44 116 1391 Fragments arranged by size: 1391 116 44 Bsp1286I G_dGCh'C Cuts at: 0 211 279 536 763 967 1056 1551 Size: 211 68 257 227 204 89 495 Fragments arranged by size: 495 257 227 211 204 89 68 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 611 1551 Size: 611 940 BsrBI CCG'CTC Cuts at: 0 56 290 1551 Size: 56 234 1261 Fragments arranged by size: 1261 234 56 BsrFI r'CCGG_y Cuts at: 0 33 309 375 832 1551 Size: 33 276 66 457 719 Fragments arranged by size: 719 457 276 66 33 BssSI C'ACGA_G Cuts at: 0 454 1551 Size: 454 1097 BstEII G'GTnAC_C Cuts at: 0 657 1551 Size: 657 894 BstXI CCAn_nnnn'nTGG Cuts at: 0 505 1551 Size: 505 1046 BstYI r'GATC_y Cuts at: 0 741 1551 Size: 741 810 BtgI C'CryG_G Cuts at: 0 174 746 1551 Size: 174 572 805 Fragments arranged by size: 805 572 174 BtsI GCAGTG_nn' Cuts at: 0 307 469 876 1551 Size: 307 162 407 675 Fragments arranged by size: 675 407 307 162 EaeI y'GGCC_r Cuts at: 0 495 570 1456 1551 Size: 495 75 886 95 Fragments arranged by size: 886 495 95 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 371 539 1489 1551 Size: 371 168 950 62 Fragments arranged by size: 950 371 168 62 EcoICRI GAG'CTC Cuts at: 0 965 1551 Size: 965 586 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 951 1068 1136 1417 1428 1522 1551 Size: 142 809 117 68 281 11 94 29 Fragments arranged by size: 809 281 142 117 94 68 29 11 EcoO109I rG'GnC_Cy Cuts at: 0 6 102 222 533 1551 Size: 6 96 120 311 1018 Fragments arranged by size: 1018 311 120 96 6 HaeII r_GCGC'y Cuts at: 0 134 149 310 338 476 1549 1551 Size: 134 15 161 28 138 1073 2 Fragments arranged by size: 1073 161 138 134 28 15 2 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 22 54 903 935 1551 Size: 22 32 849 32 616 Fragments arranged by size: 849 616 32 32 22 KasI G'GCGC_C Cuts at: 0 130 306 1551 Size: 130 176 1245 Fragments arranged by size: 1245 176 130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 236 1412 1551 Size: 236 1176 139 Fragments arranged by size: 1176 236 139 MscI TGG'CCA Cuts at: 0 1458 1551 Size: 1458 93 MslI CAynn'nnrTG Cuts at: 0 1247 1263 1337 1353 1551 Size: 1247 16 74 16 198 Fragments arranged by size: 1247 198 74 16 16 MspA1I CmG'CkG Cuts at: 0 176 611 827 1448 1542 1551 Size: 176 435 216 621 94 9 Fragments arranged by size: 621 435 216 176 94 9 NaeI GCC'GGC Cuts at: 0 35 1551 Size: 35 1516 NarI GG'CG_CC Cuts at: 0 131 307 1551 Size: 131 176 1244 Fragments arranged by size: 1244 176 131 NcoI C'CATG_G Cuts at: 0 746 1551 Size: 746 805 NgoMIV G'CCGG_C Cuts at: 0 33 1551 Size: 33 1518 NsiI A_TGCA'T Cuts at: 0 910 1517 1551 Size: 910 607 34 Fragments arranged by size: 910 607 34 NspI r_CATG'y Cuts at: 0 1515 1551 Size: 1515 36 PasI CC'CwG_GG Cuts at: 0 255 1551 Size: 255 1296 PfoI T'CCnGG_A Cuts at: 0 26 98 1551 Size: 26 72 1453 Fragments arranged by size: 1453 72 26 PmlI CAC'GTG Cuts at: 0 20 1551 Size: 20 1531 PpuMI rG'GwC_Cy Cuts at: 0 6 102 222 1551 Size: 6 96 120 1329 Fragments arranged by size: 1329 120 96 6 PspOMI G'GGCC_C Cuts at: 0 532 1551 Size: 532 1019 PvuII CAG'CTG Cuts at: 0 611 827 1448 1542 1551 Size: 611 216 621 94 9 Fragments arranged by size: 621 611 216 94 9 RsrII CG'GwC_CG Cuts at: 0 1385 1551 Size: 1385 166 SacI G_AGCT'C Cuts at: 0 967 1551 Size: 967 584 SacII CC_GC'GG Cuts at: 0 177 1551 Size: 177 1374 SanDI GG'GwC_CC Cuts at: 0 102 1551 Size: 102 1449 ScaI AGT'ACT Cuts at: 0 781 1551 Size: 781 770 SfcI C'TryA_G Cuts at: 0 73 864 1551 Size: 73 791 687 Fragments arranged by size: 791 687 73 SfoI GGC'GCC Cuts at: 0 132 308 1551 Size: 132 176 1243 Fragments arranged by size: 1243 176 132 SgrAI Cr'CCGG_yG Cuts at: 0 309 1551 Size: 309 1242 SmaI CCC'GGG Cuts at: 0 107 332 1551 Size: 107 225 1219 Fragments arranged by size: 1219 225 107 SmlI C'TyrA_G Cuts at: 0 616 1551 Size: 616 935 StyI C'CwwG_G Cuts at: 0 746 1551 Size: 746 805 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 56 247 529 757 1551 Size: 56 191 282 228 794 Fragments arranged by size: 794 282 228 191 56 TatI w'GTAC_w Cuts at: 0 779 1551 Size: 779 772 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1539 1551 Size: 1539 12 XhoI C'TCGA_G Cuts at: 0 616 1551 Size: 616 935 XmaI C'CCGG_G Cuts at: 0 105 330 1551 Size: 105 225 1221 Fragments arranged by size: 1221 225 105 XmnI GAAnn'nnTTC Cuts at: 0 1293 1348 1551 Size: 1293 55 203 Fragments arranged by size: 1293 203 55 Enzymes that do cut: AcuI AfeI AflIII AleI AlwNI ApaI AvaI BamHI BanI BanII BbeI BbsI BbvCI BfrBI BglI Bme1580I BmgBI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI BstEII BstXI BstYI BtgI BtsI EaeI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PfoI PmlI PpuMI PspOMI PvuII RsrII SacI SacII SanDI ScaI SfcI SfoI SgrAI SmaI SmlI StyI TaqII TatI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BglII BlpI BmrI BmtI BplI BpuEI BsaBI BsaWI BsiWI BspEI BspHI BsrDI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI PacI PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI SalI SapI SbfI SexAI SfiI SgfI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI ZraI