Restriction map for
pF1KE2188
(Linear) (Six-base) MAPSORT of: KIEE2188.seq Check: 8635 from: 1 to: 1269
KIEE2188.orf
With 182 enzymes: SgfI *
June 2, 2011 17:00 ..
AccI GT'mk_AC
Cuts at: 0 123 674 988 1269
Size: 123 551 314 281
Fragments arranged by size:
551 314 281 123
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 562 633 1269
Size: 237 325 71 636
Fragments arranged by size:
636 325 237 71
AleI CACnn'nnGTG
Cuts at: 0 778 1167 1269
Size: 778 389 102
Fragments arranged by size:
778 389 102
AlwNI CAG_nnn'CTG
Cuts at: 0 208 271 1269
Size: 208 63 998
Fragments arranged by size:
998 208 63
ApaI G_GGCC'C
Cuts at: 0 24 863 1269
Size: 24 839 406
Fragments arranged by size:
839 406 24
ApoI r'AATT_y
Cuts at: 0 1016 1269
Size: 1016 253
BanII G_rGCy'C
Cuts at: 0 24 46 311 863 1269
Size: 24 22 265 552 406
Fragments arranged by size:
552 406 265 24 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 21 1269
Size: 21 1248
BbvCI CC'TCA_GC
Cuts at: 0 359 1269
Size: 359 910
BglII A'GATC_T
Cuts at: 0 1157 1269
Size: 1157 112
Bme1580I G_kGCm'C
Cuts at: 0 24 863 1269
Size: 24 839 406
Fragments arranged by size:
839 406 24
BmgBI CAC'GTC
Cuts at: 0 27 1269
Size: 27 1242
BmrI ACTGGGnnnn_n'
Cuts at: 0 954 1099 1269
Size: 954 145 170
Fragments arranged by size:
954 170 145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 952 1099 1224 1269
Size: 367 585 147 125 45
Fragments arranged by size:
585 367 147 125 45
Bpu10I CC'TnA_GC
Cuts at: 0 359 941 1187 1269
Size: 359 582 246 82
Fragments arranged by size:
582 359 246 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 696 1269
Size: 505 191 573
Fragments arranged by size:
573 505 191
BsaI GGTCTCn'nnnn_
Cuts at: 0 615 1269
Size: 615 654
BsaAI yAC'GTr
Cuts at: 0 776 1269
Size: 776 493
BsaHI Gr'CG_yC
Cuts at: 0 975 1269
Size: 975 294
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 817 847 1197 1227 1269
Size: 817 30 350 30 42
Fragments arranged by size:
817 350 42 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 376 1269
Size: 151 225 893
Fragments arranged by size:
893 225 151
BseYI C'CCAG_C
Cuts at: 0 95 497 706 746 815 1083 1269
Size: 95 402 209 40 69 268 186
Fragments arranged by size:
402 268 209 186 95 69 40
BsiEI CG_ry'CG
Cuts at: 0 56 878 1269
Size: 56 822 391
Fragments arranged by size:
822 391 56
BsiHKAI G_wGCw'C
Cuts at: 0 764 1269
Size: 764 505
BsmBI CGTCTCn'nnnn_
Cuts at: 0 982 1269
Size: 982 287
Bsp1286I G_dGCh'C
Cuts at: 0 24 46 311 764 863 1269
Size: 24 22 265 453 99 406
Fragments arranged by size:
453 406 265 99 24 22
BsrBI CCG'CTC
Cuts at: 0 1238 1269
Size: 1238 31
BstEII G'GTnAC_C
Cuts at: 0 219 1269
Size: 219 1050
BstYI r'GATC_y
Cuts at: 0 1157 1269
Size: 1157 112
BtgI C'CryG_G
Cuts at: 0 401 1269
Size: 401 868
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 792 1269
Size: 792 477
BtsI GCAGTG_nn'
Cuts at: 0 471 579 1269
Size: 471 108 690
Fragments arranged by size:
690 471 108
EaeI y'GGCC_r
Cuts at: 0 193 437 617 1176 1269
Size: 193 244 180 559 93
Fragments arranged by size:
559 244 193 180 93
EarI CTCTTCn'nnn_
Cuts at: 0 436 769 1269
Size: 436 333 500
Fragments arranged by size:
500 436 333
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1057 1269
Size: 1057 212
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 367 562 633 952 1099 1224 1269
Size: 237 130 195 71 319 147 125 45
Fragments arranged by size:
319 237 195 147 130 125 71 45
EcoO109I rG'GnC_Cy
Cuts at: 0 20 819 859 1208 1269
Size: 20 799 40 349 61
Fragments arranged by size:
799 349 61 40 20
EcoRI G'AATT_C
Cuts at: 0 1016 1269
Size: 1016 253
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 105 137 229 261 352 384 817 849
Size: 105 32 92 32 91 32 433 32
Cuts at: 849 1269
Size: 420
Fragments arranged by size:
433 420 105 92 91 32 32 32
32
HincII GTy'rAC
Cuts at: 0 989 1269
Size: 989 280
MscI TGG'CCA
Cuts at: 0 195 439 1178 1269
Size: 195 244 739 91
Fragments arranged by size:
739 244 195 91
MslI CAynn'nnrTG
Cuts at: 0 778 919 1167 1178 1263 1269
Size: 778 141 248 11 85 6
Fragments arranged by size:
778 248 141 85 11 6
MspA1I CmG'CkG
Cuts at: 0 99 501 653 1269
Size: 99 402 152 616
Fragments arranged by size:
616 402 152 99
NspI r_CATG'y
Cuts at: 0 72 1269
Size: 72 1197
PasI CC'CwG_GG
Cuts at: 0 156 1269
Size: 156 1113
PflMI CCAn_nnn'nTGG
Cuts at: 0 1012 1269
Size: 1012 257
PmlI CAC'GTG
Cuts at: 0 776 1269
Size: 776 493
PpuMI rG'GwC_Cy
Cuts at: 0 819 1208 1269
Size: 819 389 61
Fragments arranged by size:
819 389 61
PspOMI G'GGCC_C
Cuts at: 0 20 859 1269
Size: 20 839 410
Fragments arranged by size:
839 410 20
PstI C_TGCA'G
Cuts at: 0 586 1269
Size: 586 683
PvuII CAG'CTG
Cuts at: 0 99 501 653 1269
Size: 99 402 152 616
Fragments arranged by size:
616 402 152 99
SalI G'TCGA_C
Cuts at: 0 987 1269
Size: 987 282
SapI GCTCTTCn'nnn_
Cuts at: 0 769 1269
Size: 769 500
SfcI C'TryA_G
Cuts at: 0 582 1269
Size: 582 687
SmlI C'TyrA_G
Cuts at: 0 520 711 1269
Size: 520 191 558
Fragments arranged by size:
558 520 191
SspI AAT'ATT
Cuts at: 0 906 1269
Size: 906 363
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 89 109 511 768 864 1269
Size: 89 20 402 257 96 405
Fragments arranged by size:
405 402 257 96 89 20
TatI w'GTAC_w
Cuts at: 0 286 1269
Size: 286 983
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 796 828 1197 1229 1269
Size: 796 32 369 32 40
Fragments arranged by size:
796 369 40 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 153 447 1269
Size: 153 294 822
Fragments arranged by size:
822 294 153
Enzymes that do cut:
AccI AcuI AleI AlwNI ApaI ApoI BanII BbsI
BbvCI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BsrBI BstEII BstYI BtgI BtgZI BtsI
EaeI EarI EciI Eco57MI EcoO109I EcoRI Hin4I HincII
MscI MslI MspA1I NspI PasI PflMI PmlI PpuMI
PspOMI PstI PvuII SalI SapI SfcI SmlI SspI
TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI
BfrBI BglI BlpI BmtI BplI BsaBI BsaWI BsgI
BsiWI BsmI BspEI BspHI BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
StyI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI