Restriction map for
pF1KE2142
(Linear) (Six-base) MAPSORT of: KIEE2142.seq Check: 775 from: 1 to: 2886
KIEE2142.orf
With 182 enzymes: SgfI *
May 26, 2015 10:14 ..
Acc65I G'GTAC_C
Cuts at: 0 1772 2509 2886
Size: 1772 737 377
Fragments arranged by size:
1772 737 377
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1450 2886
Size: 1450 1436
AfeI AGC'GCT
Cuts at: 0 757 2886
Size: 757 2129
AflIII A'CryG_T
Cuts at: 0 1562 2469 2886
Size: 1562 907 417
Fragments arranged by size:
1562 907 417
AhdI GACnn_n'nnGTC
Cuts at: 0 1695 2886
Size: 1695 1191
AleI CACnn'nnGTG
Cuts at: 0 2326 2886
Size: 2326 560
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1399 1431 2886
Size: 1399 32 1455
Fragments arranged by size:
1455 1399 32
AlwNI CAG_nnn'CTG
Cuts at: 0 177 664 1152 1464 2557 2886
Size: 177 487 488 312 1093 329
Fragments arranged by size:
1093 488 487 329 312 177
ApaI G_GGCC'C
Cuts at: 0 427 1796 2692 2816 2886
Size: 427 1369 896 124 70
Fragments arranged by size:
1369 896 427 124 70
ApaLI G'TGCA_C
Cuts at: 0 16 1744 2886
Size: 16 1728 1142
Fragments arranged by size:
1728 1142 16
AvaI C'yCGr_G
Cuts at: 0 536 577 584 614 751 1364 1759 1789
Size: 536 41 7 30 137 613 395 30
Cuts at: 1789 1981 2809 2886
Size: 192 828 77
Fragments arranged by size:
828 613 536 395 192 137 77 41
30 30 7
BanI G'GyrC_C
Cuts at: 0 101 255 414 481 966 1103 1250 1396
Size: 101 154 159 67 485 137 147 146
Cuts at: 1396 1755 1772 2406 2509 2737 2886
Size: 359 17 634 103 228 149
Fragments arranged by size:
634 485 359 228 159 154 149 147
146 137 103 101 67 17
BanII G_rGCy'C
Cuts at: 0 220 427 584 1427 1511 1591 1790 1796
Size: 220 207 157 843 84 80 199 6
Cuts at: 1796 2692 2816 2886
Size: 896 124 70
Fragments arranged by size:
896 843 220 207 199 157 124 84
80 70 6
BbeI G_GCGC'C
Cuts at: 0 105 970 1254 2741 2886
Size: 105 865 284 1487 145
Fragments arranged by size:
1487 865 284 145 105
BbsI GAAGACnn'nnnn_
Cuts at: 0 1545 2324 2886
Size: 1545 779 562
Fragments arranged by size:
1545 779 562
BbvCI CC'TCA_GC
Cuts at: 0 547 1241 1354 1601 2864 2886
Size: 547 694 113 247 1263 22
Fragments arranged by size:
1263 694 547 247 113 22
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 928 962 2886
Size: 928 34 1924
Fragments arranged by size:
1924 928 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2692 2886
Size: 2692 194
BglI GCCn_nnn'nGGC
Cuts at: 0 238 653 1062 1132 1264 2642 2820 2886
Size: 238 415 409 70 132 1378 178 66
Fragments arranged by size:
1378 415 409 238 178 132 70 66
BlpI GC'TnA_GC
Cuts at: 0 2255 2858 2886
Size: 2255 603 28
Fragments arranged by size:
2255 603 28
Bme1580I G_kGCm'C
Cuts at: 0 20 87 132 419 427 486 1269 1487
Size: 20 67 45 287 8 59 783 218
Cuts at: 1487 1748 1760 1796 2692 2816 2886
Size: 261 12 36 896 124 70
Fragments arranged by size:
896 783 287 261 218 124 70 67
59 45 36 20 12 8
BmgBI CAC'GTC
Cuts at: 0 1565 2886
Size: 1565 1321
BmrI ACTGGGnnnn_n'
Cuts at: 0 514 2886
Size: 514 2372
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 562 2886
Size: 562 2324
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 142 190 289 853 2059 2332 2418
Size: 109 33 48 99 564 1206 273 86
Cuts at: 2418 2886
Size: 468
Fragments arranged by size:
1206 564 468 273 109 99 86 48
33
Bpu10I CC'TnA_GC
Cuts at: 0 547 719 827 1073 1241 1354 1601 2864
Size: 547 172 108 246 168 113 247 1263
Cuts at: 2864 2886
Size: 22
Fragments arranged by size:
1263 547 247 246 172 168 113 108
22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2659 2886
Size: 2659 227
BsaI GGTCTCn'nnnn_
Cuts at: 0 268 355 2155 2886
Size: 268 87 1800 731
Fragments arranged by size:
1800 731 268 87
BsaAI yAC'GTr
Cuts at: 0 640 1743 2096 2472 2886
Size: 640 1103 353 376 414
Fragments arranged by size:
1103 640 414 376 353
BsaBI GATnn'nnATC
Cuts at: 0 1438 2886
Size: 1438 1448
BsaHI Gr'CG_yC
Cuts at: 0 102 967 1251 1535 2464 2738 2843 2886
Size: 102 865 284 284 929 274 105 43
Fragments arranged by size:
929 865 284 284 274 105 102 43
BsaWI w'CCGG_w
Cuts at: 0 1910 2714 2886
Size: 1910 804 172
Fragments arranged by size:
1910 804 172
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1504 1534 2686 2716 2886
Size: 1504 30 1152 30 170
Fragments arranged by size:
1504 1152 170 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 286 304 876 1885 1999 2886
Size: 286 18 572 1009 114 887
Fragments arranged by size:
1009 887 572 286 114 18
BseYI C'CCAG_C
Cuts at: 0 131 595 621 977 1817 2685 2788 2815
Size: 131 464 26 356 840 868 103 27
Cuts at: 2815 2886
Size: 71
Fragments arranged by size:
868 840 464 356 131 103 71 27
26
BsiEI CG_ry'CG
Cuts at: 0 26 1136 2299 2886
Size: 26 1110 1163 587
Fragments arranged by size:
1163 1110 587 26
BsiHKAI G_wGCw'C
Cuts at: 0 20 1591 1748 2339 2586 2886
Size: 20 1571 157 591 247 300
Fragments arranged by size:
1571 591 300 247 157 20
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1999 2886
Size: 1999 887
Bsp1286I G_dGCh'C
Cuts at: 0 20 87 132 220 419 427 486 584
Size: 20 67 45 88 199 8 59 98
Cuts at: 584 1269 1427 1487 1511 1591 1748 1760 1790
Size: 685 158 60 24 80 157 12 30
Cuts at: 1790 1796 2339 2586 2692 2816 2886
Size: 6 543 247 106 124 70
Fragments arranged by size:
685 543 247 199 158 157 124 106
98 88 80 70 67 60 59 45
30 24 20 12 8 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1151 1940 2886
Size: 1151 789 946
Fragments arranged by size:
1151 946 789
BsrBI CCG'CTC
Cuts at: 0 99 743 942 2724 2886
Size: 99 644 199 1782 162
Fragments arranged by size:
1782 644 199 162 99
BsrDI GCAATG_nn'
Cuts at: 0 18 2283 2886
Size: 18 2265 603
Fragments arranged by size:
2265 603 18
BsrFI r'CCGG_y
Cuts at: 0 491 1010 2050 2074 2133 2216 2886
Size: 491 519 1040 24 59 83 670
Fragments arranged by size:
1040 670 519 491 83 59 24
BssHII G'CGCG_C
Cuts at: 0 2178 2380 2539 2886
Size: 2178 202 159 347
Fragments arranged by size:
2178 347 202 159
BssSI C'ACGA_G
Cuts at: 0 1471 2425 2886
Size: 1471 954 461
Fragments arranged by size:
1471 954 461
BstAPI GCAn_nnn'nTGC
Cuts at: 0 177 481 501 1659 2886
Size: 177 304 20 1158 1227
Fragments arranged by size:
1227 1158 304 177 20
BstEII G'GTnAC_C
Cuts at: 0 369 390 2886
Size: 369 21 2496
Fragments arranged by size:
2496 369 21
BstXI CCAn_nnnn'nTGG
Cuts at: 0 36 139 188 2886
Size: 36 103 49 2698
Fragments arranged by size:
2698 103 49 36
Bsu36I CC'TnA_GG
Cuts at: 0 1082 1576 2576 2886
Size: 1082 494 1000 310
Fragments arranged by size:
1082 1000 494 310
BtgI C'CryG_G
Cuts at: 0 419 1109 1234 1449 1528 2249 2324 2675
Size: 419 690 125 215 79 721 75 351
Cuts at: 2675 2705 2731 2825 2886
Size: 30 26 94 61
Fragments arranged by size:
721 690 419 351 215 125 94 79
75 61 30 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2175 2886
Size: 2175 711
BtsI GCAGTG_nn'
Cuts at: 0 171 558 682 1219 1267 1809 2886
Size: 171 387 124 537 48 542 1077
Fragments arranged by size:
1077 542 537 387 171 124 48
DraIII CAC_nnn'GTG
Cuts at: 0 736 1110 1403 1476 2886
Size: 736 374 293 73 1410
Fragments arranged by size:
1410 736 374 293 73
DrdI GACnn_nn'nnGTC
Cuts at: 0 1375 2886
Size: 1375 1511
EaeI y'GGCC_r
Cuts at: 0 23 239 524 690 738 803 851 1091
Size: 23 216 285 166 48 65 48 240
Cuts at: 1091 1133 1287 2886
Size: 42 154 1599
Fragments arranged by size:
1599 285 240 216 166 154 65 48
48 42 23
EagI C'GGCC_G
Cuts at: 0 23 1133 2886
Size: 23 1110 1753
Fragments arranged by size:
1753 1110 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 949 2279 2886
Size: 949 1330 607
Fragments arranged by size:
1330 949 607
EcoICRI GAG'CTC
Cuts at: 0 1589 2886
Size: 1589 1297
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 142 190 289 853 1450 2059 2332
Size: 109 33 48 99 564 597 609 273
Cuts at: 2332 2418 2886
Size: 86 468
Fragments arranged by size:
609 597 564 468 273 109 99 86
48 33
EcoO109I rG'GnC_Cy
Cuts at: 0 294 466 574 661 715 882 896 1068
Size: 294 172 108 87 54 167 14 172
Cuts at: 1068 1793 2303 2572 2799 2886
Size: 725 510 269 227 87
Fragments arranged by size:
725 510 294 269 227 172 172 167
108 87 87 54 14
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2559 2790 2886
Size: 2559 231 96
Fragments arranged by size:
2559 231 96
FspI TGC'GCA
Cuts at: 0 1273 2116 2200 2275 2886
Size: 1273 843 84 75 611
Fragments arranged by size:
1273 843 611 84 75
HaeII r_GCGC'y
Cuts at: 0 105 759 970 1026 1046 1254 1824 2057
Size: 105 654 211 56 20 208 570 233
Cuts at: 2057 2741 2886
Size: 684 145
Fragments arranged by size:
684 654 570 233 211 208 145 105
56 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1681 1713 2686 2718 2886
Size: 1681 32 973 32 168
Fragments arranged by size:
1681 973 168 32 32
KasI G'GCGC_C
Cuts at: 0 101 966 1250 2737 2886
Size: 101 865 284 1487 149
Fragments arranged by size:
1487 865 284 149 101
KpnI G_GTAC'C
Cuts at: 0 1776 2513 2886
Size: 1776 737 373
Fragments arranged by size:
1776 737 373
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2387 2509 2886
Size: 2387 122 377
Fragments arranged by size:
2387 377 122
MscI TGG'CCA
Cuts at: 0 241 526 692 1093 2886
Size: 241 285 166 401 1793
Fragments arranged by size:
1793 401 285 241 166
MslI CAynn'nnrTG
Cuts at: 0 2326 2886
Size: 2326 560
MspA1I CmG'CkG
Cuts at: 0 120 198 808 1209 1236 1844 2554 2827
Size: 120 78 610 401 27 608 710 273
Cuts at: 2827 2886
Size: 59
Fragments arranged by size:
710 610 608 401 273 120 78 59
27
NaeI GCC'GGC
Cuts at: 0 2052 2076 2886
Size: 2052 24 810
Fragments arranged by size:
2052 810 24
NarI GG'CG_CC
Cuts at: 0 102 967 1251 2738 2886
Size: 102 865 284 1487 148
Fragments arranged by size:
1487 865 284 148 102
NcoI C'CATG_G
Cuts at: 0 1449 2249 2675 2886
Size: 1449 800 426 211
Fragments arranged by size:
1449 800 426 211
NgoMIV G'CCGG_C
Cuts at: 0 2050 2074 2886
Size: 2050 24 812
Fragments arranged by size:
2050 812 24
NotI GC'GGCC_GC
Cuts at: 0 1133 2886
Size: 1133 1753
NspI r_CATG'y
Cuts at: 0 630 1657 2886
Size: 630 1027 1229
Fragments arranged by size:
1229 1027 630
PasI CC'CwG_GG
Cuts at: 0 1797 2886
Size: 1797 1089
PflMI CCAn_nnn'nTGG
Cuts at: 0 643 664 736 2886
Size: 643 21 72 2150
Fragments arranged by size:
2150 643 72 21
PfoI T'CCnGG_A
Cuts at: 0 206 1316 1619 2886
Size: 206 1110 303 1267
Fragments arranged by size:
1267 1110 303 206
PmlI CAC'GTG
Cuts at: 0 640 1743 2472 2886
Size: 640 1103 729 414
Fragments arranged by size:
1103 729 640 414
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1861 1893 2886
Size: 1861 32 993
Fragments arranged by size:
1861 993 32
PpuMI rG'GwC_Cy
Cuts at: 0 294 715 1068 2886
Size: 294 421 353 1818
Fragments arranged by size:
1818 421 353 294
PshAI GACnn'nnGTC
Cuts at: 0 367 1549 2886
Size: 367 1182 1337
Fragments arranged by size:
1337 1182 367
PspOMI G'GGCC_C
Cuts at: 0 423 1792 2688 2812 2886
Size: 423 1369 896 124 74
Fragments arranged by size:
1369 896 423 124 74
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1720 1752 2886
Size: 1720 32 1134
Fragments arranged by size:
1720 1134 32
PstI C_TGCA'G
Cuts at: 0 1148 1460 1664 2063 2156 2396 2480 2751
Size: 1148 312 204 399 93 240 84 271
Cuts at: 2751 2886
Size: 135
Fragments arranged by size:
1148 399 312 271 240 204 135 93
84
PvuII CAG'CTG
Cuts at: 0 120 198 1844 2554 2886
Size: 120 78 1646 710 332
Fragments arranged by size:
1646 710 332 120 78
SacI G_AGCT'C
Cuts at: 0 1591 2886
Size: 1591 1295
SacII CC_GC'GG
Cuts at: 0 1237 2828 2886
Size: 1237 1591 58
Fragments arranged by size:
1591 1237 58
SanDI GG'GwC_CC
Cuts at: 0 1068 2886
Size: 1068 1818
SbfI CC_TGCA'GG
Cuts at: 0 2396 2886
Size: 2396 490
SexAI A'CCwGG_T
Cuts at: 0 732 2886
Size: 732 2154
SfcI C'TryA_G
Cuts at: 0 115 1144 1456 1660 1808 2059 2152 2392
Size: 115 1029 312 204 148 251 93 240
Cuts at: 2392 2476 2747 2886
Size: 84 271 139
Fragments arranged by size:
1029 312 271 251 240 204 148 139
115 93 84
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 238 653 1132 2820 2886
Size: 238 415 479 1688 66
Fragments arranged by size:
1688 479 415 238 66
SfoI GGC'GCC
Cuts at: 0 103 968 1252 2739 2886
Size: 103 865 284 1487 147
Fragments arranged by size:
1487 865 284 147 103
SgrAI Cr'CCGG_yG
Cuts at: 0 2050 2886
Size: 2050 836
SmaI CCC'GGG
Cuts at: 0 586 1366 1761 1791 2811 2886
Size: 586 780 395 30 1020 75
Fragments arranged by size:
1020 780 586 395 75 30
SmlI C'TyrA_G
Cuts at: 0 536 2638 2886
Size: 536 2102 248
Fragments arranged by size:
2102 536 248
SphI G_CATG'C
Cuts at: 0 630 1657 2886
Size: 630 1027 1229
Fragments arranged by size:
1229 1027 630
SrfI GCCC'GGGC
Cuts at: 0 1791 2886
Size: 1791 1095
StuI AGG'CCT
Cuts at: 0 232 867 2758 2886
Size: 232 635 1891 128
Fragments arranged by size:
1891 635 232 128
StyI C'CwwG_G
Cuts at: 0 365 651 1449 1884 2249 2534 2567 2675
Size: 365 286 798 435 365 285 33 108
Cuts at: 2675 2886
Size: 211
Fragments arranged by size:
798 435 365 365 286 285 211 108
33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 321 2179 2886
Size: 321 1858 707
Fragments arranged by size:
1858 707 321
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1525 1557 2886
Size: 1525 32 1329
Fragments arranged by size:
1525 1329 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2682 2886
Size: 2682 204
XhoI C'TCGA_G
Cuts at: 0 536 2886
Size: 536 2350
XmaI C'CCGG_G
Cuts at: 0 584 1364 1759 1789 2809 2886
Size: 584 780 395 30 1020 77
Fragments arranged by size:
1020 780 584 395 77 30
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AhdI AleI AloI AlwNI
ApaI ApaLI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI
Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI
EciI EcoICRI Eco57MI EcoO109I FalI FspI HaeII Hin4I
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PasI PflMI PfoI PmlI
PpiI PpuMI PshAI PspOMI PsrI PstI PvuII SacI
SacII SanDI SbfI SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI SphI SrfI StuI StyI TaqII TstI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AlfI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BclI BfrBI
BglII BmtI BsgI BsiWI BsmI BspEI BspHI BsrGI
BstBI BstYI BstZ17I ClaI CspCI DraI EarI EcoNI
EcoRI EcoRV FseI FspAI HincII HindIII HpaI MfeI
MluI NdeI NheI NruI NsiI PacI PciI PmeI
PsiI PspXI PvuI RsrII SalI SapI ScaI SgfI
SnaBI SpeI SspI SwaI TatI Tth111I XbaI XmnI
ZraI