Restriction map for
pF1KE2133
(Linear) (Six-base) MAPSORT of: KIEE2133.seq Check: 3479 from: 1 to: 2979
KIEE2133.orf
With 182 enzymes: SgfI *
February 28, 2012 13:55 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2306 2979
Size: 2306 673
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1383 1415 2979
Size: 1383 32 1564
Fragments arranged by size:
1564 1383 32
ApaLI G'TGCA_C
Cuts at: 0 2040 2979
Size: 2040 939
ApoI r'AATT_y
Cuts at: 0 1824 2125 2199 2271 2398 2407 2979
Size: 1824 301 74 72 127 9 572
Fragments arranged by size:
1824 572 301 127 74 72 9
AvaI C'yCGr_G
Cuts at: 0 187 874 2498 2979
Size: 187 687 1624 481
Fragments arranged by size:
1624 687 481 187
BanII G_rGCy'C
Cuts at: 0 214 848 2979
Size: 214 634 2131
Fragments arranged by size:
2131 634 214
BbsI GAAGACnn'nnnn_
Cuts at: 0 188 513 2979
Size: 188 325 2466
Fragments arranged by size:
2466 325 188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1316 1350 2979
Size: 1316 34 1629
Fragments arranged by size:
1629 1316 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 970 1192 1360 2979
Size: 970 222 168 1619
Fragments arranged by size:
1619 970 222 168
BclI T'GATC_A
Cuts at: 0 95 2979
Size: 95 2884
BglII A'GATC_T
Cuts at: 0 84 741 2358 2979
Size: 84 657 1617 621
Fragments arranged by size:
1617 657 621 84
Bme1580I G_kGCm'C
Cuts at: 0 2044 2979
Size: 2044 935
BmrI ACTGGGnnnn_n'
Cuts at: 0 718 1001 2979
Size: 718 283 1978
Fragments arranged by size:
1978 718 283
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1514 1987 2979
Size: 1514 473 992
Fragments arranged by size:
1514 992 473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 1577 2979
Size: 434 1143 1402
Fragments arranged by size:
1402 1143 434
Bpu10I CC'TnA_GC
Cuts at: 0 213 2963 2979
Size: 213 2750 16
Fragments arranged by size:
2750 213 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1301 1969 2772 2979
Size: 1301 668 803 207
Fragments arranged by size:
1301 803 668 207
BsaI GGTCTCn'nnnn_
Cuts at: 0 195 2423 2979
Size: 195 2228 556
Fragments arranged by size:
2228 556 195
BsaAI yAC'GTr
Cuts at: 0 1145 2979
Size: 1145 1834
BsaBI GATnn'nnATC
Cuts at: 0 746 2979
Size: 746 2233
BsaHI Gr'CG_yC
Cuts at: 0 539 1348 2979
Size: 539 809 1631
Fragments arranged by size:
1631 809 539
BsaWI w'CCGG_w
Cuts at: 0 973 2676 2979
Size: 973 1703 303
Fragments arranged by size:
1703 973 303
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 383 746 892 1645 1740 2344 2979
Size: 383 363 146 753 95 604 635
Fragments arranged by size:
753 635 604 383 363 146 95
BseYI C'CCAG_C
Cuts at: 0 1997 2001 2031 2774 2979
Size: 1997 4 30 743 205
Fragments arranged by size:
1997 743 205 30 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1404 2979
Size: 1404 1575
BsiEI CG_ry'CG
Cuts at: 0 578 2979
Size: 578 2401
BsiHKAI G_wGCw'C
Cuts at: 0 214 1325 2044 2979
Size: 214 1111 719 935
Fragments arranged by size:
1111 935 719 214
BsmI GAATG_Cn'
Cuts at: 0 724 2672 2979
Size: 724 1948 307
Fragments arranged by size:
1948 724 307
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1567 2979
Size: 1567 1412
Bsp1286I G_dGCh'C
Cuts at: 0 214 848 1325 2044 2979
Size: 214 634 477 719 935
Fragments arranged by size:
935 719 634 477 214
BspHI T'CATG_A
Cuts at: 0 663 2507 2979
Size: 663 1844 472
Fragments arranged by size:
1844 663 472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2036 2979
Size: 2036 943
BsrDI GCAATG_nn'
Cuts at: 0 62 766 2544 2979
Size: 62 704 1778 435
Fragments arranged by size:
1778 704 435 62
BsrFI r'CCGG_y
Cuts at: 0 3 1744 2979
Size: 3 1741 1235
Fragments arranged by size:
1741 1235 3
BssHII G'CGCG_C
Cuts at: 0 13 2979
Size: 13 2966
BssSI C'ACGA_G
Cuts at: 0 2011 2979
Size: 2011 968
BstAPI GCAn_nnn'nTGC
Cuts at: 0 719 2979
Size: 719 2260
BstEII G'GTnAC_C
Cuts at: 0 1023 1740 2979
Size: 1023 717 1239
Fragments arranged by size:
1239 1023 717
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2394 2979
Size: 2394 585
BstYI r'GATC_y
Cuts at: 0 84 741 2358 2979
Size: 84 657 1617 621
Fragments arranged by size:
1617 657 621 84
BtgI C'CryG_G
Cuts at: 0 161 380 834 2460 2979
Size: 161 219 454 1626 519
Fragments arranged by size:
1626 519 454 219 161
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2872 2979
Size: 2872 107
BtsI GCAGTG_nn'
Cuts at: 0 268 2979
Size: 268 2711
DraI TTT'AAA
Cuts at: 0 1119 1586 2718 2979
Size: 1119 467 1132 261
Fragments arranged by size:
1132 1119 467 261
DraIII CAC_nnn'GTG
Cuts at: 0 2653 2979
Size: 2653 326
DrdI GACnn_nn'nnGTC
Cuts at: 0 544 2979
Size: 544 2435
EaeI y'GGCC_r
Cuts at: 0 24 2435 2797 2877 2979
Size: 24 2411 362 80 102
Fragments arranged by size:
2411 362 102 80 24
EarI CTCTTCn'nnn_
Cuts at: 0 999 1088 1421 1663 2321 2979
Size: 999 89 333 242 658 658
Fragments arranged by size:
999 658 658 333 242 89
EcoICRI GAG'CTC
Cuts at: 0 212 2979
Size: 212 2767
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 1577 2306 2979
Size: 434 1143 729 673
Fragments arranged by size:
1143 729 673 434
EcoO109I rG'GnC_Cy
Cuts at: 0 1597 2979
Size: 1597 1382
EcoRI G'AATT_C
Cuts at: 0 2271 2979
Size: 2271 708
EcoRV GAT'ATC
Cuts at: 0 2505 2979
Size: 2505 474
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 527 2380 2979
Size: 527 1853 599
Fragments arranged by size:
1853 599 527
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 830 862 1140 1172 1609 1641 1898 1930
Size: 830 32 278 32 437 32 257 32
Cuts at: 1930 2239 2271 2599 2631 2979
Size: 309 32 328 32 348
Fragments arranged by size:
830 437 348 328 309 278 257 32
32 32 32 32 32
MfeI C'AATT_G
Cuts at: 0 1640 2979
Size: 1640 1339
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2543 2979
Size: 2543 436
MslI CAynn'nnrTG
Cuts at: 0 2761 2979
Size: 2761 218
MspA1I CmG'CkG
Cuts at: 0 30 36 238 634 2001 2838 2979
Size: 30 6 202 396 1367 837 141
Fragments arranged by size:
1367 837 396 202 141 30 6
NaeI GCC'GGC
Cuts at: 0 5 2979
Size: 5 2974
NcoI C'CATG_G
Cuts at: 0 161 380 834 2979
Size: 161 219 454 2145
Fragments arranged by size:
2145 454 219 161
NdeI CA'TA_TG
Cuts at: 0 2619 2979
Size: 2619 360
NgoMIV G'CCGG_C
Cuts at: 0 3 2979
Size: 3 2976
NspI r_CATG'y
Cuts at: 0 2211 2979
Size: 2211 768
PasI CC'CwG_GG
Cuts at: 0 292 2979
Size: 292 2687
PsiI TTA'TAA
Cuts at: 0 1048 2761 2979
Size: 1048 1713 218
Fragments arranged by size:
1713 1048 218
PspXI vC'TCGA_Gb
Cuts at: 0 874 2979
Size: 874 2105
PvuI CG_AT'CG
Cuts at: 0 578 2979
Size: 578 2401
PvuII CAG'CTG
Cuts at: 0 30 634 2001 2838 2979
Size: 30 604 1367 837 141
Fragments arranged by size:
1367 837 604 141 30
SacI G_AGCT'C
Cuts at: 0 214 2979
Size: 214 2765
ScaI AGT'ACT
Cuts at: 0 1497 1765 2979
Size: 1497 268 1214
Fragments arranged by size:
1497 1214 268
SfcI C'TryA_G
Cuts at: 0 1726 2741 2979
Size: 1726 1015 238
Fragments arranged by size:
1726 1015 238
SmlI C'TyrA_G
Cuts at: 0 874 1316 1984 2498 2787 2979
Size: 874 442 668 514 289 192
Fragments arranged by size:
874 668 514 442 289 192
SphI G_CATG'C
Cuts at: 0 2211 2979
Size: 2211 768
SspI AAT'ATT
Cuts at: 0 461 2019 2627 2979
Size: 461 1558 608 352
Fragments arranged by size:
1558 608 461 352
StyI C'CwwG_G
Cuts at: 0 161 380 834 1557 2979
Size: 161 219 454 723 1422
Fragments arranged by size:
1422 723 454 219 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 576 1473 2473 2979
Size: 576 897 1000 506
Fragments arranged by size:
1000 897 576 506
TatI w'GTAC_w
Cuts at: 0 1495 1694 1763 2979
Size: 1495 199 69 1216
Fragments arranged by size:
1495 1216 199 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 673 705 1469 1501 2979
Size: 673 32 764 32 1478
Fragments arranged by size:
1478 764 673 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 705 1379 1385 2979
Size: 705 674 6 1594
Fragments arranged by size:
1594 705 674 6
XhoI C'TCGA_G
Cuts at: 0 874 2498 2979
Size: 874 1624 481
Fragments arranged by size:
1624 874 481
XmnI GAAnn'nnTTC
Cuts at: 0 2126 2360 2979
Size: 2126 234 619
Fragments arranged by size:
2126 619 234
Enzymes that do cut:
AcuI AloI ApaLI ApoI AvaI BanII BbsI BcgI
BciVI BclI BglII Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I MfeI
MmeI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI
PasI PsiI PspXI PvuI PvuII SacI ScaI SfcI
SmlI SphI SspI StyI TaqII TatI TstI XcmI
XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BfrBI
BglI BlpI BmgBI BmtI BsaXI BsiWI BspEI BsrBI
BsrGI BstBI BstZ17I Bsu36I ClaI CspCI EagI EciI
EcoNI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MluI MscI NarI NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PsrI PstI RsrII SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI StuI SwaI Tth111I XbaI
XmaI ZraI