Restriction map for
pF1KE2129
(Linear) (Six-base) MAPSORT of: KIEE2129.seq Check: 6628 from: 1 to: 2793
KIEE2129.orf
With 182 enzymes: SgfI *
March 14, 2016 17:24 ..
AclI AA'CG_TT
Cuts at: 0 374 2793
Size: 374 2419
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 1037 1805 2078 2353 2671 2793
Size: 581 456 768 273 275 318 122
Fragments arranged by size:
768 581 456 318 275 273 122
AflIII A'CryG_T
Cuts at: 0 257 2793
Size: 257 2536
AhdI GACnn_n'nnGTC
Cuts at: 0 1369 2793
Size: 1369 1424
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 21 53 2793
Size: 21 32 2740
Fragments arranged by size:
2740 32 21
ApaI G_GGCC'C
Cuts at: 0 26 1552 2793
Size: 26 1526 1241
Fragments arranged by size:
1526 1241 26
ApoI r'AATT_y
Cuts at: 0 1126 2092 2227 2432 2793
Size: 1126 966 135 205 361
Fragments arranged by size:
1126 966 361 205 135
AseI AT'TA_AT
Cuts at: 0 821 2793
Size: 821 1972
AvaI C'yCGr_G
Cuts at: 0 44 476 2793
Size: 44 432 2317
Fragments arranged by size:
2317 432 44
BanI G'GyrC_C
Cuts at: 0 2298 2793
Size: 2298 495
BanII G_rGCy'C
Cuts at: 0 13 26 1552 2518 2793
Size: 13 13 1526 966 275
Fragments arranged by size:
1526 966 275 13 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 125 2456 2726 2793
Size: 125 2331 270 67
Fragments arranged by size:
2331 270 125 67
BclI T'GATC_A
Cuts at: 0 1068 2177 2793
Size: 1068 1109 616
Fragments arranged by size:
1109 1068 616
BglII A'GATC_T
Cuts at: 0 811 2793
Size: 811 1982
Bme1580I G_kGCm'C
Cuts at: 0 26 1552 2492 2793
Size: 26 1526 940 301
Fragments arranged by size:
1526 940 301 26
BmrI ACTGGGnnnn_n'
Cuts at: 0 328 2793
Size: 328 2465
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 792 1042 2793
Size: 586 206 250 1751
Fragments arranged by size:
1751 586 250 206
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 2658 2793
Size: 442 2216 135
Fragments arranged by size:
2216 442 135
BsaBI GATnn'nnATC
Cuts at: 0 2528 2793
Size: 2528 265
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 21 51 1327 1357 1709 1739 2793
Size: 21 30 1276 30 352 30 1054
Fragments arranged by size:
1276 1054 352 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 307 2278 2793
Size: 307 1971 515
Fragments arranged by size:
1971 515 307
BseYI C'CCAG_C
Cuts at: 0 111 2793
Size: 111 2682
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1026 2793
Size: 1026 1767
BsiEI CG_ry'CG
Cuts at: 0 1077 2793
Size: 1077 1716
BsiHKAI G_wGCw'C
Cuts at: 0 2518 2793
Size: 2518 275
BsmI GAATG_Cn'
Cuts at: 0 364 408 1383 2793
Size: 364 44 975 1410
Fragments arranged by size:
1410 975 364 44
Bsp1286I G_dGCh'C
Cuts at: 0 13 26 1552 2492 2518 2793
Size: 13 13 1526 940 26 275
Fragments arranged by size:
1526 940 275 26 13 13
BspHI T'CATG_A
Cuts at: 0 744 881 1319 1373 2445 2793
Size: 744 137 438 54 1072 348
Fragments arranged by size:
1072 744 438 348 137 54
BsrDI GCAATG_nn'
Cuts at: 0 771 909 1622 2793
Size: 771 138 713 1171
Fragments arranged by size:
1171 771 713 138
BsrFI r'CCGG_y
Cuts at: 0 197 2793
Size: 197 2596
BstAPI GCAn_nnn'nTGC
Cuts at: 0 770 2793
Size: 770 2023
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1359 2793
Size: 1359 1434
BstYI r'GATC_y
Cuts at: 0 175 811 873 2793
Size: 175 636 62 1920
Fragments arranged by size:
1920 636 175 62
BtgI C'CryG_G
Cuts at: 0 2762 2793
Size: 2762 31
BtsI GCAGTG_nn'
Cuts at: 0 425 681 976 2793
Size: 425 256 295 1817
Fragments arranged by size:
1817 425 295 256
DraI TTT'AAA
Cuts at: 0 1097 2793
Size: 1097 1696
EaeI y'GGCC_r
Cuts at: 0 422 920 1742 2793
Size: 422 498 822 1051
Fragments arranged by size:
1051 822 498 422
EarI CTCTTCn'nnn_
Cuts at: 0 2009 2793
Size: 2009 784
EcoICRI GAG'CTC
Cuts at: 0 2516 2793
Size: 2516 277
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 586 792 1037 1042 1805 2078 2353
Size: 581 5 206 245 5 763 273 275
Cuts at: 2353 2671 2793
Size: 318 122
Fragments arranged by size:
763 581 318 275 273 245 206 122
5 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 718 2793
Size: 718 2075
EcoO109I rG'GnC_Cy
Cuts at: 0 23 1548 1549 1758 2793
Size: 23 1525 1 209 1035
Fragments arranged by size:
1525 1035 209 23 1
EcoRV GAT'ATC
Cuts at: 0 1874 2793
Size: 1874 919
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 866 2793
Size: 866 1927
HaeII r_GCGC'y
Cuts at: 0 53 2793
Size: 53 2740
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1053 1085 1355 1387 2743 2775 2793
Size: 1053 32 270 32 1356 32 18
Fragments arranged by size:
1356 1053 270 32 32 32 18
HincII GTy'rAC
Cuts at: 0 378 2793
Size: 378 2415
HindIII A'AGCT_T
Cuts at: 0 1854 2335 2793
Size: 1854 481 458
Fragments arranged by size:
1854 481 458
HpaI GTT'AAC
Cuts at: 0 378 2793
Size: 378 2415
MfeI C'AATT_G
Cuts at: 0 1799 2793
Size: 1799 994
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 969 2793
Size: 969 1824
MscI TGG'CCA
Cuts at: 0 424 922 1744 2793
Size: 424 498 822 1049
Fragments arranged by size:
1049 822 498 424
MslI CAynn'nnrTG
Cuts at: 0 334 1927 2793
Size: 334 1593 866
Fragments arranged by size:
1593 866 334
MspA1I CmG'CkG
Cuts at: 0 64 141 316 1384 1446 2793
Size: 64 77 175 1068 62 1347
Fragments arranged by size:
1347 1068 175 77 64 62
NcoI C'CATG_G
Cuts at: 0 2762 2793
Size: 2762 31
NspI r_CATG'y
Cuts at: 0 261 1584 1651 2793
Size: 261 1323 67 1142
Fragments arranged by size:
1323 1142 261 67
PciI A'CATG_T
Cuts at: 0 257 2793
Size: 257 2536
PflMI CCAn_nnn'nTGG
Cuts at: 0 2246 2793
Size: 2246 547
PfoI T'CCnGG_A
Cuts at: 0 2192 2384 2793
Size: 2192 192 409
Fragments arranged by size:
2192 409 192
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 21 53 440 472 922 954 2793
Size: 21 32 387 32 450 32 1839
Fragments arranged by size:
1839 450 387 32 32 32 21
PpuMI rG'GwC_Cy
Cuts at: 0 1758 2793
Size: 1758 1035
PspOMI G'GGCC_C
Cuts at: 0 22 1548 2793
Size: 22 1526 1245
Fragments arranged by size:
1526 1245 22
PstI C_TGCA'G
Cuts at: 0 1871 2606 2793
Size: 1871 735 187
Fragments arranged by size:
1871 735 187
PvuII CAG'CTG
Cuts at: 0 316 1384 1446 2793
Size: 316 1068 62 1347
Fragments arranged by size:
1347 1068 316 62
SacI G_AGCT'C
Cuts at: 0 2518 2793
Size: 2518 275
SanDI GG'GwC_CC
Cuts at: 0 1758 2793
Size: 1758 1035
SfcI C'TryA_G
Cuts at: 0 287 409 1805 1867 2602 2793
Size: 287 122 1396 62 735 191
Fragments arranged by size:
1396 735 287 191 122 62
SgrAI Cr'CCGG_yG
Cuts at: 0 197 2793
Size: 197 2596
SmaI CCC'GGG
Cuts at: 0 46 2793
Size: 46 2747
SmlI C'TyrA_G
Cuts at: 0 457 476 2673 2793
Size: 457 19 2197 120
Fragments arranged by size:
2197 457 120 19
SphI G_CATG'C
Cuts at: 0 1651 2793
Size: 1651 1142
SspI AAT'ATT
Cuts at: 0 927 1561 2793
Size: 927 634 1232
Fragments arranged by size:
1232 927 634
StuI AGG'CCT
Cuts at: 0 836 2793
Size: 836 1957
StyI C'CwwG_G
Cuts at: 0 1553 2762 2793
Size: 1553 1209 31
Fragments arranged by size:
1553 1209 31
TatI w'GTAC_w
Cuts at: 0 2598 2793
Size: 2598 195
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1327 1359 1709 1741 2793
Size: 1327 32 350 32 1052
Fragments arranged by size:
1327 1052 350 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 432 2793
Size: 432 2361
XhoI C'TCGA_G
Cuts at: 0 476 2793
Size: 476 2317
XmaI C'CCGG_G
Cuts at: 0 44 2793
Size: 44 2749
XmnI GAAnn'nnTTC
Cuts at: 0 452 536 937 2525 2793
Size: 452 84 401 1588 268
Fragments arranged by size:
1588 452 401 268 84
Enzymes that do cut:
AclI AcuI AflIII AhdI AloI ApaI ApoI AseI
AvaI BanI BanII BbsI BclI BglII Bme1580I BmrI
BpmI BpuEI BsaBI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrFI BstAPI BstXI
BstYI BtgI BtsI DraI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII
HpaI MfeI MmeI MscI MslI MspA1I NcoI NspI
PciI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII
SacI SanDI SfcI SgrAI SmaI SmlI SphI SspI
StuI StyI TatI TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AleI
AlfI AlwNI ApaLI AscI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BfrBI BglI BlpI BmgBI
BmtI BplI Bpu10I BsaI BsaAI BsaHI BsaWI BsiWI
BsmBI BspEI BspMI BsrBI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI
EagI EciI EcoRI FseI FspI FspAI KasI KpnI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SnaBI SpeI SrfI SwaI
TaqII Tth111I XbaI ZraI