Restriction map for
pF1KE2112
(Linear) (Six-base) MAPSORT of: KIEE2112.seq Check: 6796 from: 1 to: 2763
KIEE2112.orf
With 182 enzymes: SgfI *
February 10, 2017 14:54 ..
AccI GT'mk_AC
Cuts at: 0 2594 2763
Size: 2594 169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 825 1482 1678 1853 2126 2404 2763
Size: 825 657 196 175 273 278 359
Fragments arranged by size:
825 657 359 278 273 196 175
AfeI AGC'GCT
Cuts at: 0 343 2763
Size: 343 2420
AflIII A'CryG_T
Cuts at: 0 2536 2763
Size: 2536 227
AlwNI CAG_nnn'CTG
Cuts at: 0 642 1477 2763
Size: 642 835 1286
Fragments arranged by size:
1286 835 642
ApaI G_GGCC'C
Cuts at: 0 292 2763
Size: 292 2471
ApaLI G'TGCA_C
Cuts at: 0 745 2763
Size: 745 2018
ApoI r'AATT_y
Cuts at: 0 1349 2140 2763
Size: 1349 791 623
Fragments arranged by size:
1349 791 623
AseI AT'TA_AT
Cuts at: 0 2228 2763
Size: 2228 535
AvaI C'yCGr_G
Cuts at: 0 855 2763
Size: 855 1908
AvrII C'CTAG_G
Cuts at: 0 1600 2763
Size: 1600 1163
BanI G'GyrC_C
Cuts at: 0 51 153 272 316 766 2763
Size: 51 102 119 44 450 1997
Fragments arranged by size:
1997 450 119 102 51 44
BanII G_rGCy'C
Cuts at: 0 292 628 941 1586 2763
Size: 292 336 313 645 1177
Fragments arranged by size:
1177 645 336 313 292
BbeI G_GCGC'C
Cuts at: 0 55 157 276 2763
Size: 55 102 119 2487
Fragments arranged by size:
2487 119 102 55
BbsI GAAGACnn'nnnn_
Cuts at: 0 74 2684 2763
Size: 74 2610 79
Fragments arranged by size:
2610 79 74
BciVI GTATCCnnnnn_n'
Cuts at: 0 2152 2763
Size: 2152 611
BclI T'GATC_A
Cuts at: 0 2133 2763
Size: 2133 630
BglI GCCn_nnn'nGGC
Cuts at: 0 113 889 961 2763
Size: 113 776 72 1802
Fragments arranged by size:
1802 776 113 72
BglII A'GATC_T
Cuts at: 0 1086 1493 1919 2763
Size: 1086 407 426 844
Fragments arranged by size:
1086 844 426 407
BlpI GC'TnA_GC
Cuts at: 0 940 1861 2763
Size: 940 921 902
Fragments arranged by size:
940 921 902
Bme1580I G_kGCm'C
Cuts at: 0 292 749 2763
Size: 292 457 2014
Fragments arranged by size:
2014 457 292
BmrI ACTGGGnnnn_n'
Cuts at: 0 370 1509 2763
Size: 370 1139 1254
Fragments arranged by size:
1254 1139 370
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 165 1248 2763
Size: 165 1083 1515
Fragments arranged by size:
1515 1083 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 160 415 499 1129 2637 2763
Size: 138 22 255 84 630 1508 126
Fragments arranged by size:
1508 630 255 138 126 84 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 2472 2763
Size: 484 1988 291
Fragments arranged by size:
1988 484 291
BsaAI yAC'GTr
Cuts at: 0 561 2763
Size: 561 2202
BsaHI Gr'CG_yC
Cuts at: 0 52 125 154 273 353 530 2763
Size: 52 73 29 119 80 177 2233
Fragments arranged by size:
2233 177 119 80 73 52 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2313 2343 2763
Size: 2313 30 420
Fragments arranged by size:
2313 420 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 76 349 352 412 2763
Size: 73 3 273 3 60 2351
Fragments arranged by size:
2351 273 73 60 3 3
BseYI C'CCAG_C
Cuts at: 0 1341 2743 2763
Size: 1341 1402 20
Fragments arranged by size:
1402 1341 20
BsiEI CG_ry'CG
Cuts at: 0 784 2763
Size: 784 1979
BsiHKAI G_wGCw'C
Cuts at: 0 476 749 941 1586 1862 2126 2763
Size: 476 273 192 645 276 264 637
Fragments arranged by size:
645 637 476 276 273 264 192
BsmI GAATG_Cn'
Cuts at: 0 2519 2763
Size: 2519 244
BsmBI CGTCTCn'nnnn_
Cuts at: 0 234 619 2763
Size: 234 385 2144
Fragments arranged by size:
2144 385 234
Bsp1286I G_dGCh'C
Cuts at: 0 292 476 628 749 941 1586 1862 2126
Size: 292 184 152 121 192 645 276 264
Cuts at: 2126 2763
Size: 637
Fragments arranged by size:
645 637 292 276 264 192 184 152
121
BsrBI CCG'CTC
Cuts at: 0 780 2763
Size: 780 1983
BsrDI GCAATG_nn'
Cuts at: 0 1018 1658 2763
Size: 1018 640 1105
Fragments arranged by size:
1105 1018 640
BsrFI r'CCGG_y
Cuts at: 0 269 2763
Size: 269 2494
BssHII G'CGCG_C
Cuts at: 0 517 2763
Size: 517 2246
BstAPI GCAn_nnn'nTGC
Cuts at: 0 642 753 2763
Size: 642 111 2010
Fragments arranged by size:
2010 642 111
BstBI TT'CG_AA
Cuts at: 0 1442 2763
Size: 1442 1321
BstEII G'GTnAC_C
Cuts at: 0 39 483 2763
Size: 39 444 2280
Fragments arranged by size:
2280 444 39
BstXI CCAn_nnnn'nTGG
Cuts at: 0 714 2763
Size: 714 2049
BstYI r'GATC_y
Cuts at: 0 1041 1086 1493 1919 2763
Size: 1041 45 407 426 844
Fragments arranged by size:
1041 844 426 407 45
Bsu36I CC'TnA_GG
Cuts at: 0 1240 2763
Size: 1240 1523
BtgI C'CryG_G
Cuts at: 0 201 218 312 2763
Size: 201 17 94 2451
Fragments arranged by size:
2451 201 94 17
BtsI GCAGTG_nn'
Cuts at: 0 723 2763
Size: 723 2040
DrdI GACnn_nn'nnGTC
Cuts at: 0 1729 2763
Size: 1729 1034
EaeI y'GGCC_r
Cuts at: 0 602 781 881 953 962 1307 2763
Size: 602 179 100 72 9 345 1456
Fragments arranged by size:
1456 602 345 179 100 72 9
EagI C'GGCC_G
Cuts at: 0 781 2763
Size: 781 1982
EarI CTCTTCn'nnn_
Cuts at: 0 1055 2476 2763
Size: 1055 1421 287
Fragments arranged by size:
1421 1055 287
EcoICRI GAG'CTC
Cuts at: 0 939 1584 2763
Size: 939 645 1179
Fragments arranged by size:
1179 939 645
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 160 415 499 825 1129 1482 1678
Size: 138 22 255 84 326 304 353 196
Cuts at: 1678 1853 2126 2404 2637 2763
Size: 175 273 278 233 126
Fragments arranged by size:
353 326 304 278 273 255 233 196
175 138 126 84 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1434 2763
Size: 1434 1329
EcoO109I rG'GnC_Cy
Cuts at: 0 288 915 1597 2763
Size: 288 627 682 1166
Fragments arranged by size:
1166 682 627 288
EcoRV GAT'ATC
Cuts at: 0 2649 2763
Size: 2649 114
FspI TGC'GCA
Cuts at: 0 385 565 2763
Size: 385 180 2198
Fragments arranged by size:
2198 385 180
FspAI rTGC'GCAy
Cuts at: 0 565 2763
Size: 565 2198
HaeII r_GCGC'y
Cuts at: 0 55 105 157 276 345 2763
Size: 55 50 52 119 69 2418
Fragments arranged by size:
2418 119 69 55 52 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1078 1110 2763
Size: 1078 32 1653
Fragments arranged by size:
1653 1078 32
HincII GTy'rAC
Cuts at: 0 129 420 2391 2763
Size: 129 291 1971 372
Fragments arranged by size:
1971 372 291 129
HindIII A'AGCT_T
Cuts at: 0 1127 2763
Size: 1127 1636
HpaI GTT'AAC
Cuts at: 0 2391 2763
Size: 2391 372
KasI G'GCGC_C
Cuts at: 0 51 153 272 2763
Size: 51 102 119 2491
Fragments arranged by size:
2491 119 102 51
MscI TGG'CCA
Cuts at: 0 883 955 964 1309 2763
Size: 883 72 9 345 1454
Fragments arranged by size:
1454 883 345 72 9
MslI CAynn'nnrTG
Cuts at: 0 250 376 2763
Size: 250 126 2387
Fragments arranged by size:
2387 250 126
MspA1I CmG'CkG
Cuts at: 0 165 314 456 2763
Size: 165 149 142 2307
Fragments arranged by size:
2307 165 149 142
NaeI GCC'GGC
Cuts at: 0 271 2763
Size: 271 2492
NarI GG'CG_CC
Cuts at: 0 52 154 273 2763
Size: 52 102 119 2490
Fragments arranged by size:
2490 119 102 52
NcoI C'CATG_G
Cuts at: 0 218 2763
Size: 218 2545
NgoMIV G'CCGG_C
Cuts at: 0 269 2763
Size: 269 2494
NotI GC'GGCC_GC
Cuts at: 0 781 2763
Size: 781 1982
NspI r_CATG'y
Cuts at: 0 753 1387 2004 2214 2751 2763
Size: 753 634 617 210 537 12
Fragments arranged by size:
753 634 617 537 210 12
PasI CC'CwG_GG
Cuts at: 0 1290 2763
Size: 1290 1473
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 964 996 2763
Size: 964 32 1767
Fragments arranged by size:
1767 964 32
PpuMI rG'GwC_Cy
Cuts at: 0 915 1597 2763
Size: 915 682 1166
Fragments arranged by size:
1166 915 682
PsiI TTA'TAA
Cuts at: 0 2566 2763
Size: 2566 197
PspOMI G'GGCC_C
Cuts at: 0 288 2763
Size: 288 2475
PspXI vC'TCGA_Gb
Cuts at: 0 855 2763
Size: 855 1908
PstI C_TGCA'G
Cuts at: 0 455 647 1919 2763
Size: 455 192 1272 844
Fragments arranged by size:
1272 844 455 192
PvuII CAG'CTG
Cuts at: 0 456 2763
Size: 456 2307
SacI G_AGCT'C
Cuts at: 0 941 1586 2763
Size: 941 645 1177
Fragments arranged by size:
1177 941 645
SacII CC_GC'GG
Cuts at: 0 315 2763
Size: 315 2448
SfcI C'TryA_G
Cuts at: 0 451 643 1915 2057 2763
Size: 451 192 1272 142 706
Fragments arranged by size:
1272 706 451 192 142
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 961 2763
Size: 961 1802
SfoI GGC'GCC
Cuts at: 0 53 155 274 2763
Size: 53 102 119 2489
Fragments arranged by size:
2489 119 102 53
SmlI C'TyrA_G
Cuts at: 0 499 855 2487 2763
Size: 499 356 1632 276
Fragments arranged by size:
1632 499 356 276
SphI G_CATG'C
Cuts at: 0 2751 2763
Size: 2751 12
SspI AAT'ATT
Cuts at: 0 2093 2154 2763
Size: 2093 61 609
Fragments arranged by size:
2093 609 61
StuI AGG'CCT
Cuts at: 0 1238 1838 2763
Size: 1238 600 925
Fragments arranged by size:
1238 925 600
StyI C'CwwG_G
Cuts at: 0 8 218 1600 2025 2763
Size: 8 210 1382 425 738
Fragments arranged by size:
1382 738 425 210 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 502 1021 1528 2763
Size: 502 519 507 1235
Fragments arranged by size:
1235 519 507 502
XhoI C'TCGA_G
Cuts at: 0 855 2763
Size: 855 1908
XmnI GAAnn'nnTTC
Cuts at: 0 979 1073 2763
Size: 979 94 1690
Fragments arranged by size:
1690 979 94
Enzymes that do cut:
AccI AcuI AfeI AflIII AlwNI ApaI ApaLI ApoI
AseI AvaI AvrII BanI BanII BbeI BbsI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BsrBI BsrDI BsrFI BssHII BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI DrdI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NspI PasI PpiI PpuMI PsiI PspOMI PspXI PstI
PvuII SacI SacII SfcI SfiI SfoI SmlI SphI
SspI StuI StyI TaqII XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI AscI AsiSI BaeI BamHI BbvCI BcgI
BfrBI BmgBI BmtI Bpu10I BsaI BsaBI BsaWI BsgI
BsiWI BspEI BspHI BspMI BsrGI BssSI BstZ17I BtgZI
ClaI CspCI DraI DraIII EciI EcoRI FalI FseI
KpnI MfeI MluI MmeI NdeI NheI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PshAI PsrI
PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI
SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI TatI
TstI Tth111I XbaI XcmI XmaI ZraI