(Linear) (Six-base) MAPSORT of: KIEE2008.seq  Check: 7196  from: 1  to: 1749



KIEE2008.orf

 With 182 enzymes: SgfI * 

                        April 23, 2019 13:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     664     732    1749 
   Size:        664      68    1017

  Fragments arranged by size: 

                1017     664      68
 
AccI GT'mk_AC

Cuts at:      0      85    1637    1749 
   Size:         85    1552     112

  Fragments arranged by size: 

                1552     112      85
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     192    1451    1749 
   Size:         48     144    1259     298

  Fragments arranged by size: 

                1259     298     144      48
 
AflIII A'CryG_T

Cuts at:      0     359    1677    1749 
   Size:        359    1318      72

  Fragments arranged by size: 

                1318     359      72
 
AlwNI CAG_nnn'CTG

Cuts at:      0      23    1749 
   Size:         23    1726
 
ApoI r'AATT_y

Cuts at:      0      93     838    1749 
   Size:         93     745     911

  Fragments arranged by size: 

                 911     745      93
 
AseI AT'TA_AT

Cuts at:      0    1394    1749 
   Size:       1394     355
 
BanI G'GyrC_C

Cuts at:      0     369    1749 
   Size:        369    1380
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      54     485    1135    1749 
   Size:         54     431     650     614

  Fragments arranged by size: 

                 650     614     431      54
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     187     574    1271    1749 
   Size:        187     387     697     478

  Fragments arranged by size: 

                 697     478     387     187
 
BmtI G_CTAG'C

Cuts at:      0    1076    1749 
   Size:       1076     673
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     634    1492    1749 
   Size:        634     858     257

  Fragments arranged by size: 

                 858     634     257
 
Bpu10I CC'TnA_GC

Cuts at:      0     810    1749 
   Size:        810     939
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1609    1749 
   Size:       1609     140
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     536     566    1749 
   Size:        536      30    1183

  Fragments arranged by size: 

                1183     536      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1597    1749 
   Size:       1597     152
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253    1749 
   Size:        253    1496
 
BsmI GAATG_Cn'

Cuts at:      0    1086    1749 
   Size:       1086     663
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     664     732    1391    1749 
   Size:        664      68     659     358

  Fragments arranged by size: 

                 664     659     358      68
 
BsrBI CCG'CTC

Cuts at:      0     547    1749 
   Size:        547    1202
 
BsrDI GCAATG_nn'

Cuts at:      0     921    1749 
   Size:        921     828
 
BsrGI T'GTAC_A

Cuts at:      0    1646    1749 
   Size:       1646     103
 
BstYI r'GATC_y

Cuts at:      0     211    1749 
   Size:        211    1538
 
BstZ17I GTA'TAC

Cuts at:      0      86    1749 
   Size:         86    1663
 
BtsI GCAGTG_nn'

Cuts at:      0    1359    1749 
   Size:       1359     390
 
DraI TTT'AAA

Cuts at:      0     564    1749 
   Size:        564    1185
 
EaeI y'GGCC_r

Cuts at:      0     684    1749 
   Size:        684    1065
 
EarI CTCTTCn'nnn_

Cuts at:      0      40    1427    1749 
   Size:         40    1387     322

  Fragments arranged by size: 

                1387     322      40
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      42    1749 
   Size:         42    1707
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     192     634    1451    1492    1749 
   Size:         48     144     442     817      41     257

  Fragments arranged by size: 

                 817     442     257     144      48      41
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1689    1749 
   Size:       1689      60
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     257     289     536     568    1749 
   Size:        257      32     247      32    1181

  Fragments arranged by size: 

                1181     257     247      32      32
 
MscI TGG'CCA

Cuts at:      0     686    1749 
   Size:        686    1063
 
MslI CAynn'nnrTG

Cuts at:      0     643    1749 
   Size:        643    1106
 
NdeI CA'TA_TG

Cuts at:      0     102    1749 
   Size:        102    1647
 
NheI G'CTAG_C

Cuts at:      0    1072    1749 
   Size:       1072     677
 
NspI r_CATG'y

Cuts at:      0     342    1681    1749 
   Size:        342    1339      68

  Fragments arranged by size: 

                1339     342      68
 
PciI A'CATG_T

Cuts at:      0    1677    1749 
   Size:       1677      72
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      76    1337    1749 
   Size:         76    1261     412

  Fragments arranged by size: 

                1261     412      76
 
PpuMI rG'GwC_Cy

Cuts at:      0    1689    1749 
   Size:       1689      60
 
PstI C_TGCA'G

Cuts at:      0    1190    1749 
   Size:       1190     559
 
SapI GCTCTTCn'nnn_

Cuts at:      0      40    1749 
   Size:         40    1709
 
SfcI C'TryA_G

Cuts at:      0     734    1186    1749 
   Size:        734     452     563

  Fragments arranged by size: 

                 734     563     452
 
SphI G_CATG'C

Cuts at:      0     342    1749 
   Size:        342    1407
 
StuI AGG'CCT

Cuts at:      0      74    1749 
   Size:         74    1675
 
StyI C'CwwG_G

Cuts at:      0       8      75     629    1749 
   Size:          8      67     554    1120

  Fragments arranged by size: 

                1120     554      67       8
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     451    1749 
   Size:        451    1298
 
TatI w'GTAC_w

Cuts at:      0     617    1646    1749 
   Size:        617    1029     103

  Fragments arranged by size: 

                1029     617     103
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      77    1334    1749 
   Size:         77    1257     415

  Fragments arranged by size: 

                1257     415      77
 
XmnI GAAnn'nnTTC

Cuts at:      0    1316    1749 
   Size:       1316     433
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII    AlwNI     ApoI     AseI     BanI
     BbsI     BmrI     BmtI     BpmI   Bpu10I     BsaI    BsaXI    BseRI
     BsgI     BsmI    BspMI    BsrBI    BsrDI    BsrGI    BstYI  BstZ17I
     BtsI     DraI     EaeI     EarI     EciI  Eco57MI EcoO109I    Hin4I
     MscI     MslI     NdeI     NheI     NspI     PciI    PflMI    PpuMI
     PstI     SapI     SfcI     SphI     StuI     StyI    TaqII     TatI
     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BplI    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspEI    BspHI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI   Bsu36I     BtgI    BtgZI     ClaI    CspCI
   DraIII     DrdI     EagI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NotI     NruI     NsiI     PacI     PasI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SrfI     SspI     SwaI     TstI  Tth111I     XbaI     XhoI
     XmaI     ZraI