Restriction map for
pF1KE1913
(Linear) (Six-base) MAPSORT of: KIEE1913.seq Check: 9646 from: 1 to: 1350
KIEE1913.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 0 146 1350
Size: 146 1204
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 483 1350
Size: 237 246 867
Fragments arranged by size:
867 246 237
AhdI GACnn_n'nnGTC
Cuts at: 0 1254 1350
Size: 1254 96
AleI CACnn'nnGTG
Cuts at: 0 535 1350
Size: 535 815
AlwNI CAG_nnn'CTG
Cuts at: 0 990 1128 1350
Size: 990 138 222
Fragments arranged by size:
990 222 138
ApaI G_GGCC'C
Cuts at: 0 849 1345 1350
Size: 849 496 5
Fragments arranged by size:
849 496 5
ApaLI G'TGCA_C
Cuts at: 0 13 1350
Size: 13 1337
AvaI C'yCGr_G
Cuts at: 0 182 726 824 859 1350
Size: 182 544 98 35 491
Fragments arranged by size:
544 491 182 98 35
BamHI G'GATC_C
Cuts at: 0 1031 1350
Size: 1031 319
BanI G'GyrC_C
Cuts at: 0 771 1350
Size: 771 579
BanII G_rGCy'C
Cuts at: 0 433 545 849 866 1345 1350
Size: 433 112 304 17 479 5
Fragments arranged by size:
479 433 304 112 17 5
BbsI GAAGACnn'nnnn_
Cuts at: 0 1319 1350
Size: 1319 31
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 27 61 1002 1036 1350
Size: 27 34 941 34 314
Fragments arranged by size:
941 314 34 34 27
BciVI GTATCCnnnnn_n'
Cuts at: 0 485 1350
Size: 485 865
BglI GCCn_nnn'nGGC
Cuts at: 0 35 392 1350
Size: 35 357 958
Fragments arranged by size:
958 357 35
Bme1580I G_kGCm'C
Cuts at: 0 17 445 512 776 849 860 1345 1350
Size: 17 428 67 264 73 11 485 5
Fragments arranged by size:
485 428 264 73 67 17 11 5
BmrI ACTGGGnnnn_n'
Cuts at: 0 309 1350
Size: 309 1041
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 1350
Size: 151 1199
Bpu10I CC'TnA_GC
Cuts at: 0 231 1350
Size: 231 1119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 1066 1350
Size: 628 438 284
Fragments arranged by size:
628 438 284
BsaI GGTCTCn'nnnn_
Cuts at: 0 586 1350
Size: 586 764
BsaAI yAC'GTr
Cuts at: 0 108 177 1350
Size: 108 69 1173
Fragments arranged by size:
1173 108 69
BsaHI Gr'CG_yC
Cuts at: 0 1280 1350
Size: 1280 70
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 484 1336 1339 1350
Size: 484 852 3 11
Fragments arranged by size:
852 484 11 3
BseYI C'CCAG_C
Cuts at: 0 94 414 869 1350
Size: 94 320 455 481
Fragments arranged by size:
481 455 320 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 1168 1350
Size: 772 396 182
Fragments arranged by size:
772 396 182
BsiEI CG_ry'CG
Cuts at: 0 483 924 1289 1350
Size: 483 441 365 61
Fragments arranged by size:
483 441 365 61
BsiHKAI G_wGCw'C
Cuts at: 0 17 866 1350
Size: 17 849 484
Fragments arranged by size:
849 484 17
Bsp1286I G_dGCh'C
Cuts at: 0 17 433 445 512 545 776 849 860
Size: 17 416 12 67 33 231 73 11
Cuts at: 860 866 1345 1350
Size: 6 479 5
Fragments arranged by size:
479 416 231 73 67 33 17 12
11 6 5
BspHI T'CATG_A
Cuts at: 0 488 1350
Size: 488 862
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 755 1350
Size: 755 595
BsrBI CCG'CTC
Cuts at: 0 135 230 701 1350
Size: 135 95 471 649
Fragments arranged by size:
649 471 135 95
BsrFI r'CCGG_y
Cuts at: 0 25 289 1350
Size: 25 264 1061
Fragments arranged by size:
1061 264 25
BstXI CCAn_nnnn'nTGG
Cuts at: 0 195 1350
Size: 195 1155
BstYI r'GATC_y
Cuts at: 0 1031 1350
Size: 1031 319
BtgI C'CryG_G
Cuts at: 0 215 918 1074 1283 1350
Size: 215 703 156 209 67
Fragments arranged by size:
703 215 209 156 67
EaeI y'GGCC_r
Cuts at: 0 27 650 689 758 899 921 938 992
Size: 27 623 39 69 141 22 17 54
Cuts at: 992 1052 1115 1157 1286 1350
Size: 60 63 42 129 64
Fragments arranged by size:
623 141 129 69 64 63 60 54
42 39 27 22 17
EagI C'GGCC_G
Cuts at: 0 921 1286 1350
Size: 921 365 64
Fragments arranged by size:
921 365 64
EarI CTCTTCn'nnn_
Cuts at: 0 168 256 1289 1350
Size: 168 88 1033 61
Fragments arranged by size:
1033 168 88 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 343 1350
Size: 343 1007
EcoICRI GAG'CTC
Cuts at: 0 864 1350
Size: 864 486
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 237 483 1350
Size: 151 86 246 867
Fragments arranged by size:
867 246 151 86
EcoO109I rG'GnC_Cy
Cuts at: 0 236 669 846 1341 1342 1350
Size: 236 433 177 495 1 8
Fragments arranged by size:
495 433 236 177 8 1
FseI GG_CCGG'CC
Cuts at: 0 29 1350
Size: 29 1321
FspI TGC'GCA
Cuts at: 0 751 1350
Size: 751 599
HaeII r_GCGC'y
Cuts at: 0 619 1350
Size: 619 731
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1240 1272 1312 1344 1350
Size: 1240 32 40 32 6
Fragments arranged by size:
1240 40 32 32 6
HincII GTy'rAC
Cuts at: 0 765 1350
Size: 765 585
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1310 1350
Size: 1310 40
MscI TGG'CCA
Cuts at: 0 652 940 994 1350
Size: 652 288 54 356
Fragments arranged by size:
652 356 288 54
MslI CAynn'nnrTG
Cuts at: 0 193 535 964 1159 1350
Size: 193 342 429 195 191
Fragments arranged by size:
429 342 195 193 191
MspA1I CmG'CkG
Cuts at: 0 98 129 402 573 1076 1350
Size: 98 31 273 171 503 274
Fragments arranged by size:
503 274 273 171 98 31
NaeI GCC'GGC
Cuts at: 0 27 291 1350
Size: 27 264 1059
Fragments arranged by size:
1059 264 27
NcoI C'CATG_G
Cuts at: 0 215 1350
Size: 215 1135
NgoMIV G'CCGG_C
Cuts at: 0 25 289 1350
Size: 25 264 1061
Fragments arranged by size:
1061 264 25
NspI r_CATG'y
Cuts at: 0 963 1350
Size: 963 387
PasI CC'CwG_GG
Cuts at: 0 1338 1350
Size: 1338 12
PflMI CCAn_nnn'nTGG
Cuts at: 0 59 414 573 576 1350
Size: 59 355 159 3 774
Fragments arranged by size:
774 355 159 59 3
PfoI T'CCnGG_A
Cuts at: 0 347 1121 1350
Size: 347 774 229
Fragments arranged by size:
774 347 229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1204 1236 1350
Size: 1204 32 114
Fragments arranged by size:
1204 114 32
PpuMI rG'GwC_Cy
Cuts at: 0 669 1350
Size: 669 681
PspOMI G'GGCC_C
Cuts at: 0 845 1341 1350
Size: 845 496 9
Fragments arranged by size:
845 496 9
PspXI vC'TCGA_Gb
Cuts at: 0 182 1350
Size: 182 1168
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1000 1032 1350
Size: 1000 32 318
Fragments arranged by size:
1000 318 32
PstI C_TGCA'G
Cuts at: 0 392 1350
Size: 392 958
PvuII CAG'CTG
Cuts at: 0 129 402 573 1350
Size: 129 273 171 777
Fragments arranged by size:
777 273 171 129
SacI G_AGCT'C
Cuts at: 0 866 1350
Size: 866 484
SacII CC_GC'GG
Cuts at: 0 1077 1350
Size: 1077 273
SbfI CC_TGCA'GG
Cuts at: 0 392 1350
Size: 392 958
SexAI A'CCwGG_T
Cuts at: 0 680 1250 1350
Size: 680 570 100
Fragments arranged by size:
680 570 100
SfcI C'TryA_G
Cuts at: 0 388 1350
Size: 388 962
SmaI CCC'GGG
Cuts at: 0 728 826 1350
Size: 728 98 524
Fragments arranged by size:
728 524 98
SmlI C'TyrA_G
Cuts at: 0 182 643 1081 1350
Size: 182 461 438 269
Fragments arranged by size:
461 438 269 182
StuI AGG'CCT
Cuts at: 0 160 258 1177 1350
Size: 160 98 919 173
Fragments arranged by size:
919 173 160 98
StyI C'CwwG_G
Cuts at: 0 215 305 518 1350
Size: 215 90 213 832
Fragments arranged by size:
832 215 213 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 333 531 883 1350
Size: 333 198 352 467
Fragments arranged by size:
467 352 333 198
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 354 386 1350
Size: 354 32 964
Fragments arranged by size:
964 354 32
Tth111I GACn'n_nGTC
Cuts at: 0 223 1350
Size: 223 1127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 570 894 972 1350
Size: 570 324 78 378
Fragments arranged by size:
570 378 324 78
XhoI C'TCGA_G
Cuts at: 0 182 1350
Size: 182 1168
XmaI C'CCGG_G
Cuts at: 0 726 824 1350
Size: 726 98 526
Fragments arranged by size:
726 526 98
XmnI GAAnn'nnTTC
Cuts at: 0 496 1350
Size: 496 854
Enzymes that do cut:
AccI AcuI AhdI AleI AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbsI BcgI BciVI BglI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrFI BstXI BstYI BtgI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I FseI FspI HaeII Hin4I HincII
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PflMI PfoI PpiI PpuMI PspOMI PspXI PsrI
PstI PvuII SacI SacII SbfI SexAI SfcI SmaI
SmlI StuI StyI TaqII TstI Tth111I XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BclI BfrBI BglII BlpI BmgBI BmtI
BplI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EcoNI EcoRI EcoRV FalI FspAI HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PshAI PsiI PvuI
RsrII SalI SanDI SapI ScaI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI
XbaI ZraI