Restriction map for
pF1KE1861
(Linear) (Six-base) MAPSORT of: KIEE1861.seq Check: 9090 from: 1 to: 1140
KIEE1861.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 490 1140
Size: 490 650
AatII G_ACGT'C
Cuts at: 0 985 1140
Size: 985 155
AccI GT'mk_AC
Cuts at: 0 899 1140
Size: 899 241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 817 1140
Size: 766 51 323
Fragments arranged by size:
766 323 51
AfeI AGC'GCT
Cuts at: 0 1060 1140
Size: 1060 80
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 397 1140
Size: 397 743
ApaLI G'TGCA_C
Cuts at: 0 1107 1140
Size: 1107 33
BanII G_rGCy'C
Cuts at: 0 628 1010 1140
Size: 628 382 130
Fragments arranged by size:
628 382 130
BbsI GAAGACnn'nnnn_
Cuts at: 0 56 298 754 1140
Size: 56 242 456 386
Fragments arranged by size:
456 386 242 56
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1098 1132 1140
Size: 1098 34 8
Fragments arranged by size:
1098 34 8
BglI GCCn_nnn'nGGC
Cuts at: 0 467 1140
Size: 467 673
Bme1580I G_kGCm'C
Cuts at: 0 958 1111 1140
Size: 958 153 29
Fragments arranged by size:
958 153 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 1140
Size: 212 928
BsaHI Gr'CG_yC
Cuts at: 0 593 982 1140
Size: 593 389 158
Fragments arranged by size:
593 389 158
BseYI C'CCAG_C
Cuts at: 0 910 951 1140
Size: 910 41 189
Fragments arranged by size:
910 189 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 1069 1140
Size: 643 426 71
Fragments arranged by size:
643 426 71
BsiEI CG_ry'CG
Cuts at: 0 678 1023 1140
Size: 678 345 117
Fragments arranged by size:
678 345 117
BsiHKAI G_wGCw'C
Cuts at: 0 195 642 943 1010 1111 1140
Size: 195 447 301 67 101 29
Fragments arranged by size:
447 301 195 101 67 29
BsmI GAATG_Cn'
Cuts at: 0 313 1140
Size: 313 827
BsmBI CGTCTCn'nnnn_
Cuts at: 0 394 1140
Size: 394 746
Bsp1286I G_dGCh'C
Cuts at: 0 195 628 642 943 958 1010 1111 1140
Size: 195 433 14 301 15 52 101 29
Fragments arranged by size:
433 301 195 101 52 29 15 14
BspHI T'CATG_A
Cuts at: 0 650 1140
Size: 650 490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 490 524 665 1140
Size: 490 34 141 475
Fragments arranged by size:
490 475 141 34
BsrBI CCG'CTC
Cuts at: 0 603 1140
Size: 603 537
BsrDI GCAATG_nn'
Cuts at: 0 65 939 1140
Size: 65 874 201
Fragments arranged by size:
874 201 65
BsrFI r'CCGG_y
Cuts at: 0 673 943 1140
Size: 673 270 197
Fragments arranged by size:
673 270 197
BsrGI T'GTAC_A
Cuts at: 0 689 1140
Size: 689 451
BssHII G'CGCG_C
Cuts at: 0 502 1140
Size: 502 638
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1106 1140
Size: 1106 34
BtgI C'CryG_G
Cuts at: 0 520 720 1023 1140
Size: 520 200 303 117
Fragments arranged by size:
520 303 200 117
BtsI GCAGTG_nn'
Cuts at: 0 1065 1140
Size: 1065 75
EaeI y'GGCC_r
Cuts at: 0 89 675 821 1020 1140
Size: 89 586 146 199 120
Fragments arranged by size:
586 199 146 120 89
EagI C'GGCC_G
Cuts at: 0 675 1020 1140
Size: 675 345 120
Fragments arranged by size:
675 345 120
EcoICRI GAG'CTC
Cuts at: 0 1008 1140
Size: 1008 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 817 1140
Size: 766 51 323
Fragments arranged by size:
766 323 51
EcoO109I rG'GnC_Cy
Cuts at: 0 513 1140
Size: 513 627
FseI GG_CCGG'CC
Cuts at: 0 677 1140
Size: 677 463
FspI TGC'GCA
Cuts at: 0 931 1140
Size: 931 209
HaeII r_GCGC'y
Cuts at: 0 1062 1140
Size: 1062 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 346 378 1140
Size: 346 32 762
Fragments arranged by size:
762 346 32
HincII GTy'rAC
Cuts at: 0 861 1140
Size: 861 279
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 965 1140
Size: 348 617 175
Fragments arranged by size:
617 348 175
MscI TGG'CCA
Cuts at: 0 91 1140
Size: 91 1049
MslI CAynn'nnrTG
Cuts at: 0 649 1140
Size: 649 491
MspA1I CmG'CkG
Cuts at: 0 522 765 1140
Size: 522 243 375
Fragments arranged by size:
522 375 243
NaeI GCC'GGC
Cuts at: 0 675 1140
Size: 675 465
NcoI C'CATG_G
Cuts at: 0 720 1140
Size: 720 420
NgoMIV G'CCGG_C
Cuts at: 0 673 1140
Size: 673 467
NspI r_CATG'y
Cuts at: 0 270 755 1140
Size: 270 485 385
Fragments arranged by size:
485 385 270
PflMI CCAn_nnn'nTGG
Cuts at: 0 134 1140
Size: 134 1006
PpuMI rG'GwC_Cy
Cuts at: 0 513 1140
Size: 513 627
PstI C_TGCA'G
Cuts at: 0 208 346 493 764 1140
Size: 208 138 147 271 376
Fragments arranged by size:
376 271 208 147 138
PvuII CAG'CTG
Cuts at: 0 765 1140
Size: 765 375
SacI G_AGCT'C
Cuts at: 0 1010 1140
Size: 1010 130
SacII CC_GC'GG
Cuts at: 0 523 1140
Size: 523 617
SexAI A'CCwGG_T
Cuts at: 0 893 1140
Size: 893 247
SfcI C'TryA_G
Cuts at: 0 204 342 489 760 1140
Size: 204 138 147 271 380
Fragments arranged by size:
380 271 204 147 138
SmlI C'TyrA_G
Cuts at: 0 227 1140
Size: 227 913
SspI AAT'ATT
Cuts at: 0 20 1140
Size: 20 1120
StyI C'CwwG_G
Cuts at: 0 509 578 620 720 1140
Size: 509 69 42 100 420
Fragments arranged by size:
509 420 100 69 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 775 1140
Size: 775 365
TatI w'GTAC_w
Cuts at: 0 272 689 1140
Size: 272 417 451
Fragments arranged by size:
451 417 272
Tth111I GACn'n_nGTC
Cuts at: 0 895 1140
Size: 895 245
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 585 891 1140
Size: 585 306 249
Fragments arranged by size:
585 306 249
ZraI GAC'GTC
Cuts at: 0 983 1140
Size: 983 157
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AlfI ApaLI BanII
BbsI BcgI BglI Bme1580I BpuEI BsaHI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BtgI BtsI EaeI
EagI EcoICRI Eco57MI EcoO109I FseI FspI HaeII Hin4I
HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PflMI PpuMI PstI PvuII SacI SacII SexAI
SfcI SmlI SspI StyI TaqII TatI Tth111I XcmI
ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AhdI AleI AloI
AlwNI ApaI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I
BsaI BsaAI BsaBI BsaWI BsaXI BseRI BsiWI BspEI
BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EarI EciI EcoNI
EcoRI EcoRV FalI FspAI HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TstI XbaI
XhoI XmaI XmnI