Restriction map for
pF1KE1860
(Linear) (Six-base) MAPSORT of: KIEE1860.seq Check: 3773 from: 1 to: 1140
KIEE1860.orf
With 182 enzymes: SgfI *
March 23, 2015 09:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 165 495 1140
Size: 165 330 645
Fragments arranged by size:
645 330 165
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 1140
Size: 81 1059
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 964 1140
Size: 964 176
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 382 414 1116 1140
Size: 382 32 702 24
Fragments arranged by size:
702 382 32 24
AlwNI CAG_nnn'CTG
Cuts at: 0 1119 1140
Size: 1119 21
ApaI G_GGCC'C
Cuts at: 0 353 646 669 743 1140
Size: 353 293 23 74 397
Fragments arranged by size:
397 353 293 74 23
BanII G_rGCy'C
Cuts at: 0 353 646 669 743 1140
Size: 353 293 23 74 397
Fragments arranged by size:
397 353 293 74 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 959 1140
Size: 959 181
BbvCI CC'TCA_GC
Cuts at: 0 1120 1140
Size: 1120 20
BglI GCCn_nnn'nGGC
Cuts at: 0 316 331 811 1140
Size: 316 15 480 329
Fragments arranged by size:
480 329 316 15
BglII A'GATC_T
Cuts at: 0 110 1140
Size: 110 1030
Bme1580I G_kGCm'C
Cuts at: 0 327 353 646 669 743 1140
Size: 327 26 293 23 74 397
Fragments arranged by size:
397 327 293 74 26 23
BmrI ACTGGGnnnn_n'
Cuts at: 0 294 1140
Size: 294 846
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 718 747 1140
Size: 718 29 393
Fragments arranged by size:
718 393 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 713 996 1140
Size: 521 192 283 144
Fragments arranged by size:
521 283 192 144
Bpu10I CC'TnA_GC
Cuts at: 0 1120 1140
Size: 1120 20
BsaI GGTCTCn'nnnn_
Cuts at: 0 471 915 982 1140
Size: 471 444 67 158
Fragments arranged by size:
471 444 158 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 382 412 611 641 1140
Size: 382 30 199 30 499
Fragments arranged by size:
499 382 199 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 421 524 739 892 1140
Size: 421 103 215 153 248
Fragments arranged by size:
421 248 215 153 103
BseYI C'CCAG_C
Cuts at: 0 363 499 579 661 1006 1140
Size: 363 136 80 82 345 134
Fragments arranged by size:
363 345 136 134 82 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 984 1140
Size: 602 382 156
Fragments arranged by size:
602 382 156
BsiHKAI G_wGCw'C
Cuts at: 0 452 1140
Size: 452 688
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1 1140
Size: 1 1139
Bsp1286I G_dGCh'C
Cuts at: 0 327 353 452 646 669 743 1140
Size: 327 26 99 194 23 74 397
Fragments arranged by size:
397 327 194 99 74 26 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 165 495 1140
Size: 165 330 645
Fragments arranged by size:
645 330 165
BsrFI r'CCGG_y
Cuts at: 0 310 1140
Size: 310 830
BstAPI GCAn_nnn'nTGC
Cuts at: 0 688 1140
Size: 688 452
BstEII G'GTnAC_C
Cuts at: 0 975 1140
Size: 975 165
BstYI r'GATC_y
Cuts at: 0 110 1140
Size: 110 1030
Bsu36I CC'TnA_GG
Cuts at: 0 732 1140
Size: 732 408
BtgI C'CryG_G
Cuts at: 0 125 1140
Size: 125 1015
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 78 1140
Size: 78 1062
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 819 854 1140
Size: 819 35 286
Fragments arranged by size:
819 286 35
EaeI y'GGCC_r
Cuts at: 0 128 317 677 1140
Size: 128 189 360 463
Fragments arranged by size:
463 360 189 128
EarI CTCTTCn'nnn_
Cuts at: 0 788 1052 1140
Size: 788 264 88
Fragments arranged by size:
788 264 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 521 713 996 1140
Size: 81 440 192 283 144
Fragments arranged by size:
440 283 192 144 81
EcoNI CCTnn'n_nnAGG
Cuts at: 0 536 951 1140
Size: 536 415 189
Fragments arranged by size:
536 415 189
EcoO109I rG'GnC_Cy
Cuts at: 0 85 267 350 523 541 576 642 643
Size: 85 182 83 173 18 35 66 1
Cuts at: 643 665 666 739 1140
Size: 22 1 73 401
Fragments arranged by size:
401 182 173 85 83 73 66 35
22 18 1 1
HaeII r_GCGC'y
Cuts at: 0 462 1140
Size: 462 678
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 425 1140
Size: 317 108 715
Fragments arranged by size:
715 317 108
MscI TGG'CCA
Cuts at: 0 319 1140
Size: 319 821
MslI CAynn'nnrTG
Cuts at: 0 37 1140
Size: 37 1103
MspA1I CmG'CkG
Cuts at: 0 499 583 1140
Size: 499 84 557
Fragments arranged by size:
557 499 84
NaeI GCC'GGC
Cuts at: 0 312 1140
Size: 312 828
NgoMIV G'CCGG_C
Cuts at: 0 310 1140
Size: 310 830
PasI CC'CwG_GG
Cuts at: 0 926 1140
Size: 926 214
PflMI CCAn_nnn'nTGG
Cuts at: 0 177 1140
Size: 177 963
PfoI T'CCnGG_A
Cuts at: 0 1112 1140
Size: 1112 28
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 382 414 1140
Size: 382 32 726
Fragments arranged by size:
726 382 32
PpuMI rG'GwC_Cy
Cuts at: 0 85 523 541 576 1140
Size: 85 438 18 35 564
Fragments arranged by size:
564 438 85 35 18
PspOMI G'GGCC_C
Cuts at: 0 349 642 665 739 1140
Size: 349 293 23 74 401
Fragments arranged by size:
401 349 293 74 23
PstI C_TGCA'G
Cuts at: 0 588 1140
Size: 588 552
PvuII CAG'CTG
Cuts at: 0 583 1140
Size: 583 557
SanDI GG'GwC_CC
Cuts at: 0 85 576 1140
Size: 85 491 564
Fragments arranged by size:
564 491 85
SexAI A'CCwGG_T
Cuts at: 0 376 1140
Size: 376 764
SfcI C'TryA_G
Cuts at: 0 584 1030 1140
Size: 584 446 110
Fragments arranged by size:
584 446 110
StuI AGG'CCT
Cuts at: 0 94 674 730 1140
Size: 94 580 56 410
Fragments arranged by size:
580 410 94 56
StyI C'CwwG_G
Cuts at: 0 854 952 1140
Size: 854 98 188
Fragments arranged by size:
854 188 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 372 1140
Size: 372 768
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 611 643 1140
Size: 611 32 497
Fragments arranged by size:
611 497 32
Enzymes that do cut:
AarI AcuI AlfI AloI AlwNI ApaI BanII BbsI
BbvCI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I
BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspMI BsrFI BstAPI BstEII BstYI Bsu36I BtgI BtgZI
CspCI EaeI EarI Eco57MI EcoNI EcoO109I HaeII MmeI
MscI MslI MspA1I NaeI NgoMIV PasI PflMI PfoI
PpiI PpuMI PspOMI PstI PvuII SanDI SexAI SfcI
StuI StyI TaqII TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI
BfrBI BlpI BmgBI BmtI BpuEI BsaAI BsaBI BsaHI
BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstBI BstXI BstZ17I BtsI ClaI
DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI