Restriction map for
pF1KE1842
(Linear) (Six-base) MAPSORT of: KIEE1842.seq Check: 7145 from: 1 to: 1074
KIEE1842.orf
With 182 enzymes: SgfI *
January 9, 2015 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 62 144 623 665 1074
Size: 62 82 479 42 409
Fragments arranged by size:
479 409 82 62 42
AatII G_ACGT'C
Cuts at: 0 181 1074
Size: 181 893
AleI CACnn'nnGTG
Cuts at: 0 691 1074
Size: 691 383
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 70 1074
Size: 70 1004
ApaI G_GGCC'C
Cuts at: 0 115 289 343 380 745 1074
Size: 115 174 54 37 365 329
Fragments arranged by size:
365 329 174 115 54 37
AscI GG'CGCG_CC
Cuts at: 0 329 1074
Size: 329 745
AvaI C'yCGr_G
Cuts at: 0 101 324 361 1074
Size: 101 223 37 713
Fragments arranged by size:
713 223 101 37
BanI G'GyrC_C
Cuts at: 0 349 453 724 769 1074
Size: 349 104 271 45 305
Fragments arranged by size:
349 305 271 104 45
BanII G_rGCy'C
Cuts at: 0 64 115 289 343 380 434 491 745
Size: 64 51 174 54 37 54 57 254
Cuts at: 745 1045 1074
Size: 300 29
Fragments arranged by size:
300 254 174 64 57 54 54 51
37 29
BbeI G_GCGC'C
Cuts at: 0 353 457 773 1074
Size: 353 104 316 301
Fragments arranged by size:
353 316 301 104
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 861 895 1074
Size: 861 34 179
Fragments arranged by size:
861 179 34
BclI T'GATC_A
Cuts at: 0 476 1074
Size: 476 598
BglI GCCn_nnn'nGGC
Cuts at: 0 277 293 347 740 1074
Size: 277 16 54 393 334
Fragments arranged by size:
393 334 277 54 16
BlpI GC'TnA_GC
Cuts at: 0 991 1074
Size: 991 83
Bme1580I G_kGCm'C
Cuts at: 0 115 289 343 380 698 745 1074
Size: 115 174 54 37 318 47 329
Fragments arranged by size:
329 318 174 115 54 47 37
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 964 1074
Size: 448 516 110
Fragments arranged by size:
516 448 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 742 1074
Size: 742 332
BsaHI Gr'CG_yC
Cuts at: 0 178 350 454 770 1074
Size: 178 172 104 316 304
Fragments arranged by size:
316 304 178 172 104
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 421 451 1074
Size: 421 30 623
Fragments arranged by size:
623 421 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 511 1074
Size: 511 563
BseYI C'CCAG_C
Cuts at: 0 76 153 697 765 1074
Size: 76 77 544 68 309
Fragments arranged by size:
544 309 77 76 68
BsiEI CG_ry'CG
Cuts at: 0 11 212 281 404 469 1074
Size: 11 201 69 123 65 605
Fragments arranged by size:
605 201 123 69 65 11
BsiHKAI G_wGCw'C
Cuts at: 0 173 1045 1074
Size: 173 872 29
Fragments arranged by size:
872 173 29
BsmBI CGTCTCn'nnnn_
Cuts at: 0 169 919 1074
Size: 169 750 155
Fragments arranged by size:
750 169 155
Bsp1286I G_dGCh'C
Cuts at: 0 64 115 173 289 343 380 434 491
Size: 64 51 58 116 54 37 54 57
Cuts at: 491 698 745 1045 1074
Size: 207 47 300 29
Fragments arranged by size:
300 207 116 64 58 57 54 54
51 47 37 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 62 144 623 665 1074
Size: 62 82 479 42 409
Fragments arranged by size:
479 409 82 62 42
BsrFI r'CCGG_y
Cuts at: 0 254 406 456 526 730 1074
Size: 254 152 50 70 204 344
Fragments arranged by size:
344 254 204 152 70 50
BssHII G'CGCG_C
Cuts at: 0 329 752 1074
Size: 329 423 322
Fragments arranged by size:
423 329 322
BssSI C'ACGA_G
Cuts at: 0 172 1074
Size: 172 902
BstAPI GCAn_nnn'nTGC
Cuts at: 0 939 1074
Size: 939 135
BstEII G'GTnAC_C
Cuts at: 0 667 1074
Size: 667 407
BstXI CCAn_nnnn'nTGG
Cuts at: 0 516 1074
Size: 516 558
BtgI C'CryG_G
Cuts at: 0 31 281 290 335 1058 1074
Size: 31 250 9 45 723 16
Fragments arranged by size:
723 250 45 31 16 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 864 1074
Size: 864 210
BtsI GCAGTG_nn'
Cuts at: 0 633 1074
Size: 633 441
EaeI y'GGCC_r
Cuts at: 0 8 209 233 278 401 466 524 602
Size: 8 201 24 45 123 65 58 78
Cuts at: 602 639 1074
Size: 37 435
Fragments arranged by size:
435 201 123 78 65 58 45 37
24 8
EagI C'GGCC_G
Cuts at: 0 8 209 278 401 466 1074
Size: 8 201 69 123 65 608
Fragments arranged by size:
608 201 123 69 65 8
EarI CTCTTCn'nnn_
Cuts at: 0 538 1074
Size: 538 536
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 40 1015 1074
Size: 40 975 59
Fragments arranged by size:
975 59 40
EcoICRI GAG'CTC
Cuts at: 0 1043 1074
Size: 1043 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 964 1074
Size: 448 516 110
Fragments arranged by size:
516 448 110
EcoO109I rG'GnC_Cy
Cuts at: 0 112 286 340 377 742 1074
Size: 112 174 54 37 365 332
Fragments arranged by size:
365 332 174 112 54 37
FseI GG_CCGG'CC
Cuts at: 0 530 1074
Size: 530 544
FspI TGC'GCA
Cuts at: 0 889 973 1074
Size: 889 84 101
Fragments arranged by size:
889 101 84
HaeII r_GCGC'y
Cuts at: 0 86 197 353 457 463 523 773 1074
Size: 86 111 156 104 6 60 250 301
Fragments arranged by size:
301 250 156 111 104 86 60 6
KasI G'GCGC_C
Cuts at: 0 349 453 769 1074
Size: 349 104 316 305
Fragments arranged by size:
349 316 305 104
MslI CAynn'nnrTG
Cuts at: 0 691 883 1074
Size: 691 192 191
Fragments arranged by size:
691 192 191
MspA1I CmG'CkG
Cuts at: 0 33 153 337 471 516 806 990 1032
Size: 33 120 184 134 45 290 184 42
Cuts at: 1032 1074
Size: 42
Fragments arranged by size:
290 184 184 134 120 45 42 42
33
NaeI GCC'GGC
Cuts at: 0 256 408 458 528 732 1074
Size: 256 152 50 70 204 342
Fragments arranged by size:
342 256 204 152 70 50
NarI GG'CG_CC
Cuts at: 0 350 454 770 1074
Size: 350 104 316 304
Fragments arranged by size:
350 316 304 104
NcoI C'CATG_G
Cuts at: 0 1058 1074
Size: 1058 16
NgoMIV G'CCGG_C
Cuts at: 0 254 406 456 526 730 1074
Size: 254 152 50 70 204 344
Fragments arranged by size:
344 254 204 152 70 50
NotI GC'GGCC_GC
Cuts at: 0 401 466 1074
Size: 401 65 608
Fragments arranged by size:
608 401 65
PspOMI G'GGCC_C
Cuts at: 0 111 285 339 376 741 1074
Size: 111 174 54 37 365 333
Fragments arranged by size:
365 333 174 111 54 37
PstI C_TGCA'G
Cuts at: 0 59 620 662 1074
Size: 59 561 42 412
Fragments arranged by size:
561 412 59 42
PvuII CAG'CTG
Cuts at: 0 516 990 1032 1074
Size: 516 474 42 42
Fragments arranged by size:
516 474 42 42
SacI G_AGCT'C
Cuts at: 0 1045 1074
Size: 1045 29
SacII CC_GC'GG
Cuts at: 0 34 338 1074
Size: 34 304 736
Fragments arranged by size:
736 304 34
SfcI C'TryA_G
Cuts at: 0 55 616 658 1074
Size: 55 561 42 416
Fragments arranged by size:
561 416 55 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 277 740 1074
Size: 277 463 334
Fragments arranged by size:
463 334 277
SfoI GGC'GCC
Cuts at: 0 351 455 771 1074
Size: 351 104 316 303
Fragments arranged by size:
351 316 303 104
SgrAI Cr'CCGG_yG
Cuts at: 0 456 1074
Size: 456 618
SmaI CCC'GGG
Cuts at: 0 103 326 363 1074
Size: 103 223 37 711
Fragments arranged by size:
711 223 103 37
SmlI C'TyrA_G
Cuts at: 0 757 1074
Size: 757 317
StyI C'CwwG_G
Cuts at: 0 272 1046 1058 1074
Size: 272 774 12 16
Fragments arranged by size:
774 272 16 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 163 195 1074
Size: 163 32 879
Fragments arranged by size:
879 163 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 279 828 1074
Size: 279 549 246
Fragments arranged by size:
549 279 246
XmaI C'CCGG_G
Cuts at: 0 101 324 361 1074
Size: 101 223 37 713
Fragments arranged by size:
713 223 101 37
ZraI GAC'GTC
Cuts at: 0 179 1074
Size: 179 895
Enzymes that do cut:
AarI AatII AleI AlfI ApaI AscI AvaI BanI
BanII BbeI BcgI BclI BglI BlpI Bme1580I BpmI
BpuEI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrFI BssHII BssSI BstAPI BstEII BstXI
BtgI BtgZI BtsI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I FseI FspI HaeII KasI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI PspOMI PstI PvuII
SacI SacII SfcI SfiI SfoI SgrAI SmaI SmlI
StyI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApaLI ApoI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BciVI BfrBI BglII BmgBI
BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaWI
BsgI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI
BstBI BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EcoNI EcoRI EcoRV FalI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI NdeI
NheI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI
SexAI SgfI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI Tth111I XbaI XhoI XmnI