Restriction map for
pF1KE1803
(Linear) (Six-base) MAPSORT of: KIEE1803.seq Check: 6862 from: 1 to: 951
KIEE1803.orf
With 182 enzymes: SgfI *
May 20, 2016 17:05 ..
Acc65I G'GTAC_C
Cuts at: 0 560 951
Size: 560 391
AclI AA'CG_TT
Cuts at: 0 827 951
Size: 827 124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 951
Size: 665 286
ApaI G_GGCC'C
Cuts at: 0 85 951
Size: 85 866
ApoI r'AATT_y
Cuts at: 0 266 798 805 951
Size: 266 532 7 146
Fragments arranged by size:
532 266 146 7
AseI AT'TA_AT
Cuts at: 0 331 509 951
Size: 331 178 442
Fragments arranged by size:
442 331 178
BamHI G'GATC_C
Cuts at: 0 222 897 951
Size: 222 675 54
Fragments arranged by size:
675 222 54
BanI G'GyrC_C
Cuts at: 0 560 951
Size: 560 391
BanII G_rGCy'C
Cuts at: 0 72 85 951
Size: 72 13 866
Fragments arranged by size:
866 72 13
BglII A'GATC_T
Cuts at: 0 456 584 951
Size: 456 128 367
Fragments arranged by size:
456 367 128
BlpI GC'TnA_GC
Cuts at: 0 485 951
Size: 485 466
Bme1580I G_kGCm'C
Cuts at: 0 85 951
Size: 85 866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 875 951
Size: 331 544 76
Fragments arranged by size:
544 331 76
BsaI GGTCTCn'nnnn_
Cuts at: 0 680 882 951
Size: 680 202 69
Fragments arranged by size:
680 202 69
BsaBI GATnn'nnATC
Cuts at: 0 902 951
Size: 902 49
BsaWI w'CCGG_w
Cuts at: 0 900 951
Size: 900 51
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 483 951
Size: 61 422 468
Fragments arranged by size:
468 422 61
BseYI C'CCAG_C
Cuts at: 0 162 168 951
Size: 162 6 783
Fragments arranged by size:
783 162 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 951
Size: 73 878
Bsp1286I G_dGCh'C
Cuts at: 0 72 85 951
Size: 72 13 866
Fragments arranged by size:
866 72 13
BspEI T'CCGG_A
Cuts at: 0 900 951
Size: 900 51
BspHI T'CATG_A
Cuts at: 0 672 951
Size: 672 279
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 41 951
Size: 41 910
BsrBI CCG'CTC
Cuts at: 0 137 951
Size: 137 814
BsrDI GCAATG_nn'
Cuts at: 0 612 951
Size: 612 339
BssSI C'ACGA_G
Cuts at: 0 76 951
Size: 76 875
BstAPI GCAn_nnn'nTGC
Cuts at: 0 254 951
Size: 254 697
BstXI CCAn_nnnn'nTGG
Cuts at: 0 147 951
Size: 147 804
BstYI r'GATC_y
Cuts at: 0 222 456 584 897 951
Size: 222 234 128 313 54
Fragments arranged by size:
313 234 222 128 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 448 951
Size: 448 503
BtsI GCAGTG_nn'
Cuts at: 0 252 951
Size: 252 699
DraI TTT'AAA
Cuts at: 0 933 951
Size: 933 18
EaeI y'GGCC_r
Cuts at: 0 461 951
Size: 461 490
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 665 875 951
Size: 331 334 210 76
Fragments arranged by size:
334 331 210 76
EcoO109I rG'GnC_Cy
Cuts at: 0 81 82 951
Size: 81 1 869
Fragments arranged by size:
869 81 1
EcoRI G'AATT_C
Cuts at: 0 798 805 951
Size: 798 7 146
Fragments arranged by size:
798 146 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 511 543 848 880 951
Size: 511 32 305 32 71
Fragments arranged by size:
511 305 71 32 32
HindIII A'AGCT_T
Cuts at: 0 475 951
Size: 475 476
KpnI G_GTAC'C
Cuts at: 0 564 951
Size: 564 387
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 614 905 951
Size: 384 230 291 46
Fragments arranged by size:
384 291 230 46
MscI TGG'CCA
Cuts at: 0 463 951
Size: 463 488
MslI CAynn'nnrTG
Cuts at: 0 145 284 951
Size: 145 139 667
Fragments arranged by size:
667 145 139
PflMI CCAn_nnn'nTGG
Cuts at: 0 788 951
Size: 788 163
PspOMI G'GGCC_C
Cuts at: 0 81 951
Size: 81 870
PstI C_TGCA'G
Cuts at: 0 187 951
Size: 187 764
SfcI C'TryA_G
Cuts at: 0 183 695 951
Size: 183 512 256
Fragments arranged by size:
512 256 183
StuI AGG'CCT
Cuts at: 0 364 951
Size: 364 587
Enzymes that do cut:
Acc65I AclI AcuI ApaI ApoI AseI BamHI BanI
BanII BglII BlpI Bme1580I BpmI BsaI BsaBI BsaWI
BseRI BseYI BsgI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BssSI BstAPI BstXI BstYI BtgZI BtsI DraI
EaeI Eco57MI EcoO109I EcoRI Hin4I HindIII KpnI MmeI
MscI MslI PflMI PspOMI PstI SfcI StuI
Enzymes that do not cut:
AarI AatII AccI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AsiSI AvaI
AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaAI BsaHI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI
BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgI
ClaI CspCI DraIII DrdI EagI EarI EciI EcoICRI
EcoNI EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI MfeI MluI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI