Restriction map for
pF1KE1794
(Linear) (Six-base) MAPSORT of: KIEE1794.seq Check: 1811 from: 1 to: 927
KIEE1794.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 0 260 927
Size: 260 667
AclI AA'CG_TT
Cuts at: 0 422 927
Size: 422 505
ApaI G_GGCC'C
Cuts at: 0 580 927
Size: 580 347
ApaLI G'TGCA_C
Cuts at: 0 782 927
Size: 782 145
ApoI r'AATT_y
Cuts at: 0 110 927
Size: 110 817
BanII G_rGCy'C
Cuts at: 0 546 580 927
Size: 546 34 347
Fragments arranged by size:
546 347 34
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 124 158 927
Size: 124 34 769
Fragments arranged by size:
769 124 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 222 733 927
Size: 222 511 194
Fragments arranged by size:
511 222 194
BglII A'GATC_T
Cuts at: 0 441 927
Size: 441 486
Bme1580I G_kGCm'C
Cuts at: 0 580 720 786 927
Size: 580 140 66 141
Fragments arranged by size:
580 141 140 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 703 927
Size: 703 224
BsaAI yAC'GTr
Cuts at: 0 640 927
Size: 640 287
BsaWI w'CCGG_w
Cuts at: 0 225 927
Size: 225 702
BseYI C'CCAG_C
Cuts at: 0 870 927
Size: 870 57
BsiHKAI G_wGCw'C
Cuts at: 0 786 927
Size: 786 141
BsmI GAATG_Cn'
Cuts at: 0 420 927
Size: 420 507
Bsp1286I G_dGCh'C
Cuts at: 0 546 580 720 786 927
Size: 546 34 140 66 141
Fragments arranged by size:
546 141 140 66 34
BspHI T'CATG_A
Cuts at: 0 186 753 927
Size: 186 567 174
Fragments arranged by size:
567 186 174
BssSI C'ACGA_G
Cuts at: 0 713 927
Size: 713 214
BstEII G'GTnAC_C
Cuts at: 0 768 927
Size: 768 159
BstXI CCAn_nnnn'nTGG
Cuts at: 0 27 379 694 927
Size: 27 352 315 233
Fragments arranged by size:
352 315 233 27
BstYI r'GATC_y
Cuts at: 0 441 603 927
Size: 441 162 324
Fragments arranged by size:
441 324 162
BtgI C'CryG_G
Cuts at: 0 498 927
Size: 498 429
DraI TTT'AAA
Cuts at: 0 845 927
Size: 845 82
DraIII CAC_nnn'GTG
Cuts at: 0 640 643 927
Size: 640 3 284
Fragments arranged by size:
640 284 3
DrdI GACnn_nn'nnGTC
Cuts at: 0 37 927
Size: 37 890
EarI CTCTTCn'nnn_
Cuts at: 0 299 927
Size: 299 628
EcoO109I rG'GnC_Cy
Cuts at: 0 576 927
Size: 576 351
HaeII r_GCGC'y
Cuts at: 0 877 927
Size: 877 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 276 308 333 365 927
Size: 276 32 25 32 562
Fragments arranged by size:
562 276 32 32 25
MfeI C'AATT_G
Cuts at: 0 744 927
Size: 744 183
MslI CAynn'nnrTG
Cuts at: 0 692 927
Size: 692 235
MspA1I CmG'CkG
Cuts at: 0 48 66 927
Size: 48 18 861
Fragments arranged by size:
861 48 18
NcoI C'CATG_G
Cuts at: 0 498 927
Size: 498 429
PflMI CCAn_nnn'nTGG
Cuts at: 0 425 927
Size: 425 502
PmlI CAC'GTG
Cuts at: 0 640 927
Size: 640 287
PspOMI G'GGCC_C
Cuts at: 0 576 927
Size: 576 351
PvuII CAG'CTG
Cuts at: 0 48 66 927
Size: 48 18 861
Fragments arranged by size:
861 48 18
SspI AAT'ATT
Cuts at: 0 338 433 850 927
Size: 338 95 417 77
Fragments arranged by size:
417 338 95 77
StyI C'CwwG_G
Cuts at: 0 20 498 927
Size: 20 478 429
Fragments arranged by size:
478 429 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 238 801 927
Size: 238 563 126
Fragments arranged by size:
563 238 126
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 525 927
Size: 525 402
Enzymes that do cut:
AccI AclI ApaI ApaLI ApoI BanII BcgI BciVI
BglII Bme1580I BsaI BsaAI BsaWI BseYI BsiHKAI BsmI
Bsp1286I BspHI BssSI BstEII BstXI BstYI BtgI DraI
DraIII DrdI EarI EcoO109I HaeII Hin4I MfeI MslI
MspA1I NcoI PflMI PmlI PspOMI PvuII SspI StyI
TaqII XcmI
Enzymes that do not cut:
AarI AatII Acc65I AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BclI BfrBI BglI BlpI BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaBI BsaHI BsaXI BseRI BsgI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI EaeI EagI EciI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MluI MmeI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI